(Circular) (Six-base) MAPSORT of: pFN21AE0973.seq Check: 8179 from: 1 to: 6340 pFN21AE0973 6340 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2748 2942 4403 5787 6055 1632 Size: 1116 194 1461 1384 268 1917 Fragments arranged by size: 1917 1461 1384 1116 268 194 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 2187 2266 2769 5239 6216 1203 Size: 984 79 503 2470 977 1327 Fragments arranged by size: 2470 1327 984 977 503 79 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2263 1184 Size: 1079 5261 AlwNI CAG_nnn'CTG Cuts at: 4083 5655 4083 Size: 1572 4768 ApaI G_GGCC'C Cuts at: 3314 3314 Size: 6340 ApaLI G'TGCA_C Cuts at: 2030 3296 4397 5553 2030 Size: 1266 1101 1156 2817 Fragments arranged by size: 2817 1266 1156 1101 ApoI r'AATT_y Cuts at: 1493 2331 3008 3649 3875 4027 6136 1493 Size: 838 677 641 226 152 2109 1697 Fragments arranged by size: 2109 1697 838 677 641 226 152 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 3665 1870 Size: 73 1722 4545 Fragments arranged by size: 4545 1722 73 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2705 3661 5116 618 Size: 325 217 627 918 956 1455 1842 Fragments arranged by size: 1842 1455 956 918 627 325 217 BanII G_rGCy'C Cuts at: 1391 3314 3462 3659 1391 Size: 1923 148 197 4072 Fragments arranged by size: 4072 1923 197 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2948 3144 3336 928 Size: 971 1049 196 192 3932 Fragments arranged by size: 3932 1049 971 196 192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4531 4565 1903 Size: 34 2594 34 3678 Fragments arranged by size: 3678 2594 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 2153 4951 Size: 107 122 71 631 605 418 63 2798 Cuts at: 4951 136 Size: 1525 Fragments arranged by size: 2798 1525 631 605 418 122 107 71 63 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 2034 3300 3314 4401 5557 1790 Size: 244 1266 14 1087 1156 2573 Fragments arranged by size: 2573 1266 1156 1087 244 14 BmgBI CAC'GTC Cuts at: 1318 2035 1318 Size: 717 5623 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3157 4071 5029 Size: 416 53 83 399 24 1740 914 958 Cuts at: 5029 442 Size: 1753 Fragments arranged by size: 1753 1740 958 914 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3481 3481 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3370 3408 5000 1239 Size: 2131 38 1592 2579 Fragments arranged by size: 2579 2131 1592 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4465 5330 5628 4465 Size: 865 298 5177 Fragments arranged by size: 5177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5003 882 Size: 617 3504 2219 Fragments arranged by size: 3504 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4131 493 Size: 711 2927 2702 Fragments arranged by size: 2927 2702 711 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4147 4529 5164 275 Size: 53 83 186 936 2614 382 635 1451 Fragments arranged by size: 2614 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6115 Fragments arranged by size: 6115 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2487 1186 Size: 1301 5039 BseYI C'CCAG_C Cuts at: 2046 5543 6039 2046 Size: 3497 496 2347 Fragments arranged by size: 3497 2347 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2371 1163 Size: 573 635 5132 Fragments arranged by size: 5132 635 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 3790 4551 5579 1540 Size: 275 186 93 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 93 BsiHKAI G_wGCw'C Cuts at: 2034 3300 3462 4401 4486 5557 2034 Size: 1266 162 939 85 1071 2817 Fragments arranged by size: 2817 1266 1071 939 162 85 BsiWI C'GTAC_G Cuts at: 2190 2190 Size: 6340 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 3300 3314 3462 3659 4401 4486 Size: 399 244 1266 14 148 197 742 85 Cuts at: 4486 5557 1391 Size: 1071 2174 Fragments arranged by size: 2174 1266 1071 742 399 244 197 148 85 14 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 2415 4229 5959 2415 Size: 1814 1730 2796 Fragments arranged by size: 2796 1814 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3695 844 Size: 2851 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2019 4227 1394 Size: 429 196 2208 3507 Fragments arranged by size: 3507 2208 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 4835 5009 65 Size: 1183 3587 174 1396 Fragments arranged by size: 3587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 4984 5171 1700 Size: 110 297 2877 187 2869 Fragments arranged by size: 2877 2869 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 3257 794 Size: 2463 3877 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 3119 1184 Size: 1935 4405 BstYI r'GATC_y Cuts at: 1972 2965 3670 4037 4422 4439 5891 5977 5989 Size: 993 705 367 385 17 1452 86 12 Cuts at: 5989 6335 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 993 705 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 513 Size: 552 939 120 4729 Fragments arranged by size: 4729 939 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3258 3856 4650 4670 2221 Size: 1037 598 794 20 3891 Fragments arranged by size: 3891 1037 794 598 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2862 2897 529 Size: 35 2298 35 3972 Fragments arranged by size: 3972 2298 35 35 DraI TTT'AAA Cuts at: 3000 3546 3644 3000 Size: 546 98 5696 Fragments arranged by size: 5696 546 98 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 3787 4676 Size: 54 1425 50 275 18 261 1696 889 Cuts at: 4676 6305 8 Size: 1629 43 Fragments arranged by size: 1696 1629 1425 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 3787 1537 Size: 275 279 1696 4090 Fragments arranged by size: 4090 1696 279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2493 3094 3173 3217 3424 3467 4270 1353 Size: 1140 601 79 44 207 43 803 3423 Fragments arranged by size: 3423 1140 803 601 207 79 44 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4927 5301 5447 387 Size: 1064 138 435 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3460 3460 Size: 6340 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2748 2942 3370 3408 4403 5000 5787 Size: 393 1116 194 428 38 995 597 787 Cuts at: 5787 6055 1239 Size: 268 1524 Fragments arranged by size: 1524 1116 995 787 597 428 393 268 194 38 EcoO109I rG'GnC_Cy Cuts at: 3310 3310 Size: 6340 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 6340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2420 2420 Size: 6340 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2108 2819 5487 1678 Size: 430 711 2668 2531 Fragments arranged by size: 2668 2531 711 430 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3434 3466 4981 5013 5055 Size: 32 135 32 1862 32 1515 32 42 Cuts at: 5055 5087 1373 Size: 32 2626 Fragments arranged by size: 2626 1862 1515 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2467 2856 3684 3930 669 Size: 988 281 529 389 828 246 3079 Fragments arranged by size: 3079 988 828 529 389 281 246 HindIII A'AGCT_T Cuts at: 748 3342 3540 748 Size: 2594 198 3548 Fragments arranged by size: 3548 2594 198 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 2187 6216 2187 Size: 4029 2311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 2696 3567 5454 5638 1305 Size: 1119 272 871 1887 184 2007 Fragments arranged by size: 2007 1887 1119 871 272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4518 Fragments arranged by size: 4518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2484 2843 4298 4657 4816 518 Size: 648 1318 359 1455 359 159 2042 Fragments arranged by size: 2042 1455 1318 648 359 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2126 3756 4433 5581 5826 1606 Size: 463 57 1630 677 1148 245 2120 Fragments arranged by size: 2120 1630 1148 677 463 245 57 NaeI GCC'GGC Cuts at: 2109 2109 Size: 6340 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 6340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 2091 3787 2091 Size: 1696 4644 NspI r_CATG'y Cuts at: 1132 1229 2773 3698 5243 1132 Size: 97 1544 925 1545 2229 Fragments arranged by size: 2229 1545 1544 925 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 2769 5239 2769 Size: 2470 3870 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6340 PfoI T'CCnGG_A Cuts at: 3424 3424 Size: 6340 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspOMI G'GGCC_C Cuts at: 3310 3310 Size: 6340 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3020 3052 3087 3119 3020 Size: 32 35 32 6241 Fragments arranged by size: 6241 35 32 32 PstI C_TGCA'G Cuts at: 830 2183 2202 3692 830 Size: 1353 19 1490 3478 Fragments arranged by size: 3478 1490 1353 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 SacI G_AGCT'C Cuts at: 3462 3462 Size: 6340 SacII CC_GC'GG Cuts at: 2127 2127 Size: 6340 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SapI GCTCTTCn'nnn_ Cuts at: 3173 3467 3173 Size: 294 6046 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SfcI C'TryA_G Cuts at: 826 1045 2179 2198 3334 3688 4823 5504 5695 Size: 219 1134 19 1136 354 1135 681 191 Cuts at: 5695 826 Size: 1471 Fragments arranged by size: 1471 1136 1135 1134 681 354 219 191 19 SfiI GGCCn_nnn'nGGCC Cuts at: 2090 2090 Size: 6340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4444 5345 5607 820 Size: 197 926 2501 901 262 1553 Fragments arranged by size: 2501 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 2596 4264 5 Size: 47 2544 1668 2081 Fragments arranged by size: 2544 2081 1668 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6340 StyI C'CwwG_G Cuts at: 513 1065 2004 3779 513 Size: 552 939 1775 3074 Fragments arranged by size: 3074 1775 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 2890 3331 4367 4384 4537 4722 Size: 1255 1014 296 441 1036 17 153 185 Cuts at: 4722 5167 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1036 1014 445 441 296 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 4586 6298 Size: 274 80 33 51 1443 643 1966 1712 Cuts at: 6298 96 Size: 138 Fragments arranged by size: 1966 1712 1443 643 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3137 3169 6300 6332 3137 Size: 32 3131 32 3145 Fragments arranged by size: 3145 3131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 4469 4469 Size: 6340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PvuII RsrII SanDI SexAI SfoI SrfI SwaI XcmI