Restriction map for
pFN21AE0973
(Linear) (Six-base) MAPSORT of: KIEE0973.seq Check: 2021 from: 1 to: 1635
KIEE0973.orf
With 182 enzymes: SgfI *
July 3, 2012 16:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 937 1635
Size: 743 194 698
Fragments arranged by size:
743 698 194
AflIII A'CryG_T
Cuts at: 0 182 261 764 1635
Size: 182 79 503 871
Fragments arranged by size:
871 503 182 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 258 1635
Size: 258 1377
ApaI G_GGCC'C
Cuts at: 0 1309 1635
Size: 1309 326
ApaLI G'TGCA_C
Cuts at: 0 25 1291 1635
Size: 25 1266 344
Fragments arranged by size:
1266 344 25
ApoI r'AATT_y
Cuts at: 0 326 1003 1635
Size: 326 677 632
Fragments arranged by size:
677 632 326
BanI G'GyrC_C
Cuts at: 0 700 1635
Size: 700 935
BanII G_rGCy'C
Cuts at: 0 1309 1457 1635
Size: 1309 148 178
Fragments arranged by size:
1309 178 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 943 1139 1331 1635
Size: 943 196 192 304
Fragments arranged by size:
943 304 196 192
BglI GCCn_nnn'nGGC
Cuts at: 0 85 148 1635
Size: 85 63 1487
Fragments arranged by size:
1487 85 63
Bme1580I G_kGCm'C
Cuts at: 0 29 1295 1309 1635
Size: 29 1266 14 326
Fragments arranged by size:
1266 326 29 14
BmgBI CAC'GTC
Cuts at: 0 30 1635
Size: 30 1605
BmrI ACTGGGnnnn_n'
Cuts at: 0 1152 1635
Size: 1152 483
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1476 1635
Size: 1476 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1365 1403 1635
Size: 1365 38 232
Fragments arranged by size:
1365 232 38
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 482 1635
Size: 482 1153
BseYI C'CCAG_C
Cuts at: 0 41 1635
Size: 41 1594
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1635
Size: 366 1269
BsiEI CG_ry'CG
Cuts at: 0 89 1635
Size: 89 1546
BsiHKAI G_wGCw'C
Cuts at: 0 29 1295 1457 1635
Size: 29 1266 162 178
Fragments arranged by size:
1266 178 162 29
BsiWI C'GTAC_G
Cuts at: 0 185 1635
Size: 185 1450
Bsp1286I G_dGCh'C
Cuts at: 0 29 1295 1309 1457 1635
Size: 29 1266 14 148 178
Fragments arranged by size:
1266 178 148 29 14
BspHI T'CATG_A
Cuts at: 0 410 1635
Size: 410 1225
BsrBI CCG'CTC
Cuts at: 0 14 1635
Size: 14 1621
BsrFI r'CCGG_y
Cuts at: 0 102 1635
Size: 102 1533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1252 1635
Size: 1252 383
BstEII G'GTnAC_C
Cuts at: 0 1114 1635
Size: 1114 521
BstYI r'GATC_y
Cuts at: 0 960 1635
Size: 960 675
BtgI C'CryG_G
Cuts at: 0 119 1635
Size: 119 1516
BtsI GCAGTG_nn'
Cuts at: 0 216 1253 1635
Size: 216 1037 382
Fragments arranged by size:
1037 382 216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 857 892 1635
Size: 857 35 743
Fragments arranged by size:
857 743 35
DraI TTT'AAA
Cuts at: 0 995 1541 1635
Size: 995 546 94
Fragments arranged by size:
995 546 94
EaeI y'GGCC_r
Cuts at: 0 86 1635
Size: 86 1549
EagI C'GGCC_G
Cuts at: 0 86 1635
Size: 86 1549
EarI CTCTTCn'nnn_
Cuts at: 0 488 1089 1168 1212 1419 1462 1635
Size: 488 601 79 44 207 43 173
Fragments arranged by size:
601 488 207 173 79 44 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1635
Size: 19 1616
EcoICRI GAG'CTC
Cuts at: 0 1455 1635
Size: 1455 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 937 1365 1403 1635
Size: 743 194 428 38 232
Fragments arranged by size:
743 428 232 194 38
EcoO109I rG'GnC_Cy
Cuts at: 0 1305 1635
Size: 1305 330
EcoRV GAT'ATC
Cuts at: 0 444 1635
Size: 444 1191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 415 1635
Size: 415 1220
HaeII r_GCGC'y
Cuts at: 0 103 814 1635
Size: 103 711 821
Fragments arranged by size:
821 711 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 1635
Size: 1429 32 174
Fragments arranged by size:
1429 174 32
HincII GTy'rAC
Cuts at: 0 462 851 1635
Size: 462 389 784
Fragments arranged by size:
784 462 389
HindIII A'AGCT_T
Cuts at: 0 1337 1535 1635
Size: 1337 198 100
Fragments arranged by size:
1337 198 100
MluI A'CGCG_T
Cuts at: 0 182 1635
Size: 182 1453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 691 1562 1635
Size: 419 272 871 73
Fragments arranged by size:
871 419 272 73
MslI CAynn'nnrTG
Cuts at: 0 479 838 1635
Size: 479 359 797
Fragments arranged by size:
797 479 359
MspA1I CmG'CkG
Cuts at: 0 64 121 1635
Size: 64 57 1514
Fragments arranged by size:
1514 64 57
NaeI GCC'GGC
Cuts at: 0 104 1635
Size: 104 1531
NgoMIV G'CCGG_C
Cuts at: 0 102 1635
Size: 102 1533
NotI GC'GGCC_GC
Cuts at: 0 86 1635
Size: 86 1549
NspI r_CATG'y
Cuts at: 0 768 1635
Size: 768 867
PciI A'CATG_T
Cuts at: 0 764 1635
Size: 764 871
PfoI T'CCnGG_A
Cuts at: 0 1419 1635
Size: 1419 216
PspOMI G'GGCC_C
Cuts at: 0 1305 1635
Size: 1305 330
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 1082 1114 1635
Size: 1015 32 35 32 521
Fragments arranged by size:
1015 521 35 32 32
PstI C_TGCA'G
Cuts at: 0 178 197 1635
Size: 178 19 1438
Fragments arranged by size:
1438 178 19
SacI G_AGCT'C
Cuts at: 0 1457 1635
Size: 1457 178
SacII CC_GC'GG
Cuts at: 0 122 1635
Size: 122 1513
SapI GCTCTTCn'nnn_
Cuts at: 0 1168 1462 1635
Size: 1168 294 173
Fragments arranged by size:
1168 294 173
SfcI C'TryA_G
Cuts at: 0 174 193 1329 1635
Size: 174 19 1136 306
Fragments arranged by size:
1136 306 174 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 85 1635
Size: 85 1550
SspI AAT'ATT
Cuts at: 0 591 1635
Size: 591 1044
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 885 1326 1635
Size: 589 296 441 309
Fragments arranged by size:
589 441 309 296
TatI w'GTAC_w
Cuts at: 0 615 1635
Size: 615 1020
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1635
Size: 1132 32 471
Fragments arranged by size:
1132 471 32
Enzymes that do cut:
AcuI AflIII AlfI ApaI ApaLI ApoI BanI BanII
BbsI BglI Bme1580I BmgBI BmrI BplI BpmI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BsrBI
BsrFI BstAPI BstEII BstYI BtgI BtsI CspCI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII MluI MmeI MslI
MspA1I NaeI NgoMIV NotI NspI PciI PfoI PspOMI
PsrI PstI SacI SacII SapI SfcI SfiI SspI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsmI BsmBI BspEI BspMI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI DraIII DrdI EcoNI EcoRI FseI FspI
FspAI HpaI KasI KpnI MfeI MscI NarI NcoI
NdeI NheI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PvuI PvuII
RsrII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI