################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp718I AvrII BciVI BfrI BlnI BsaBI BsiWI BsmI BspDI BspHI BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI DraI DrdI EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI KspI MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AflIII 377 ACATGT A^C[GA][CT]GT AhdI 2269 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 2130 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 363 CCTCAGG CC^T[ACGT]AGG ApaI 563 GGGCCC GGGCC^C AspEI 2269 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1326 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 482 GGATCC G^GATCC BbeI 1674 GGCGCC GGCGC^C BbrPI 580 CACGTG CAC^GTG BbsI 2287 GTCTTC GAAGAC(2/6) BbvCI 2592 CCTCAGC CCTCAGC(-5/-2) BcgI 1113 GCAGATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1289 TGATCA T^GATCA BfaI 1526 CTAG C^TAG BglI 2597 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1128 AGATCT A^GATCT BlpI 252 GCTGAGC GC^T[ACGT]AGC Bpu1102I 252 GCTGAGC GC^T[ACGT]AGC BpuAI 2287 GTCTTC GAAGAC(2/6) BsaAI 580 CACGTG [CT]AC^GT[GA] BsaI 2664 GAGACC GGTCTC(1/5) BsgI 1387 CTGCAC GTGCAG(16/14) BsiEI 1959 CGGCCG CG[GA][CT]^CG Bsp1407I 572 TGTACA T^GTACA BspLU11I 377 ACATGT A^CATGT BsrBI 1008 CCGCTC CCGCTC(-3/-3) BsrGI 572 TGTACA T^GTACA BssSI 853 CACGAG CACGAG(-5/-1) BstEII 1698 GGTGACC G^GT[ACGT]ACC BstPI 1698 GGTGACC G^GT[ACGT]ACC BstXI 2678 CCAAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 363 CCTCAGG CC^T[ACGT]AGG CelII 252 GCTGAGC GC^T[ACGT]AGC CpoI 683 CGGTCCG CG^G[AT]CCG EagI 1959 CGGCCG C^GGCCG Eam1105I 2269 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1959 CGGCCG C^GGCCG Eco52I 1959 CGGCCG C^GGCCG Eco81I 363 CCTCAGG CC^T[ACGT]AGG EcoO65I 1698 GGTGACC G^GT[ACGT]ACC FbaI 1289 TGATCA T^GATCA HincII 2191 GTCAAC GT[CT]^[GA]AC HindII 2191 GTCAAC GT[CT]^[GA]AC KasI 1674 GGCGCC G^GCGCC MaeI 1526 CTAG C^TAG MseI 1240 TTAA T^TAA NaeI 116 GCCGGC GCC^GGC NarI 1674 GGCGCC GG^CGCC NgoMIV 116 GCCGGC G^CCGGC PflFI 1326 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 580 CACGTG CAC^GTG PmlI 580 CACGTG CAC^GTG RsrII 683 CGGTCCG CG^G[AT]CCG SacI 1921 GAGCTC GAGCT^C SapI 909 GAAGAGC GCTCTTC(1/4) SfoI 1674 GGCGCC GGC^GCC SspBI 572 TGTACA T^GTACA StuI 2027 AGGCCT AGG^CCT Tru9I 1240 TTAA T^TAA Tth111I 1326 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2174 CCAAAATCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 1485 GACGTC GACGT^C 2479 GACGTC AccIII 80 TCCGGA T^CCGGA 1322 TCCGGA Alw44I 1185 GTGCAC G^TGCAC 1566 GTGCAC Aor51HI 977 AGCGCT AGC^GCT 998 AGCGCT ApaLI 1185 GTGCAC G^TGCAC 1566 GTGCAC AviII 215 TGCGCA TGC^GCA 2378 TGCGCA BalI 1979 TGGCCA TGG^CCA 2117 TGGCCA BseAI 80 TCCGGA T^CCGGA 1322 TCCGGA BsmBI 433 GAGACG CGTCTC(1/5) 1660 GAGACG BspEI 80 TCCGGA T^CCGGA 1322 TCCGGA BspMI 1385 ACCTGC ACCTGC(4/8) 1592 ACCTGC BsrFI 116 GCCGGC [GA]^CCGG[CT] 1650 GCCGGT Cfr10I 116 GCCGGC [GA]^CCGG[CT] 1650 GCCGGT DraIII 474 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 871 CACCGCGTG Eco47III 977 AGCGCT AGC^GCT 998 AGCGCT EcoT14I 2249 CCAAGG C^C[AT][AT]GG 2490 CCTTGG FspI 215 TGCGCA TGC^GCA 2378 TGCGCA MluNI 1979 TGGCCA TGG^CCA 2117 TGGCCA MroI 80 TCCGGA T^CCGGA 1322 TCCGGA MscI 1979 TGGCCA TGG^CCA 2117 TGGCCA NspI 377 ACATGT [GA]CATG^[CT] 2690 ACATGC PstI 2350 CTGCAG CTGCA^G 2753 CTGCAG ScaI 107 AGTACT AGT^ACT 1166 AGTACT StyI 2249 CCAAGG C^C[AT][AT]GG 2490 CCTTGG Tsp509I 1740 AATT ^AATT 2220 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI