(Circular) (Six-base) MAPSORT of: pFN21AE3325.seq Check: 6411 from: 1 to: 7468 pFN21AE3325 7468 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3494 4488 5278 278 Size: 53 83 186 936 1958 994 790 2468 Fragments arranged by size: 2468 1958 994 936 790 186 83 53 AccI GT'mk_AC Cuts at: 1937 4811 1937 Size: 2874 4594 Acc65I G'GTAC_C Cuts at: 4789 4789 Size: 7468 AclI AA'CG_TT Cuts at: 1563 5595 5968 1563 Size: 4032 373 3063 Fragments arranged by size: 4032 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2101 2792 3869 4321 5531 6915 7183 1632 Size: 469 691 1077 452 1210 1384 268 1917 Fragments arranged by size: 1917 1384 1210 1077 691 469 452 268 AfeI AGC'GCT Cuts at: 2984 3005 2984 Size: 21 7447 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7271 AflIII A'CryG_T Cuts at: 1203 2382 6367 7344 1203 Size: 1179 3985 977 1327 Fragments arranged by size: 3985 1327 1179 977 AgeI A'CCGG_T Cuts at: 6299 6299 Size: 7468 AhdI GACnn_n'nnGTC Cuts at: 4279 6197 4279 Size: 1918 5550 AleI CACnn'nnGTG Cuts at: 1166 2585 2726 1166 Size: 1419 141 5908 Fragments arranged by size: 5908 1419 141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7468 AlwNI CAG_nnn'CTG Cuts at: 4140 5211 6783 4140 Size: 1071 1572 4825 Fragments arranged by size: 4825 1572 1071 ApaI G_GGCC'C Cuts at: 2572 2572 Size: 7468 ApaLI G'TGCA_C Cuts at: 3190 3571 5525 6681 3190 Size: 381 1954 1156 3977 Fragments arranged by size: 3977 1954 1156 381 ApoI r'AATT_y Cuts at: 1493 4777 5003 5155 7264 1493 Size: 3284 226 152 2109 1697 Fragments arranged by size: 3284 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6022 160 Size: 872 4990 1606 Fragments arranged by size: 4990 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7468 AvaI C'yCGr_G Cuts at: 1870 1943 2564 2614 2779 2824 3675 4793 1870 Size: 73 621 50 165 45 851 1118 4545 Fragments arranged by size: 4545 1118 851 621 165 73 50 45 BamHI G'GATC_C Cuts at: 2487 4798 5165 2487 Size: 2311 367 4790 Fragments arranged by size: 4790 2311 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2874 3652 3658 3679 4097 Size: 325 217 627 1087 778 6 21 418 Cuts at: 4097 4414 4789 6244 618 Size: 317 375 1455 1842 Fragments arranged by size: 1842 1455 1087 778 627 418 375 325 317 217 21 6 BanII G_rGCy'C Cuts at: 1391 2478 2513 2572 3930 4112 4787 1391 Size: 1087 35 59 1358 182 675 4072 Fragments arranged by size: 4072 1358 1087 675 182 59 35 BbeI G_GCGC'C Cuts at: 3683 3683 Size: 7468 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4285 928 Size: 971 2386 4111 Fragments arranged by size: 4111 2386 971 BbvCI CC'TCA_GC Cuts at: 4598 4598 Size: 7468 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3107 3141 5659 5693 1903 Size: 34 1170 34 2518 34 3678 Fragments arranged by size: 3678 2518 1170 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5360 6570 5360 Size: 1210 6258 BclI T'GATC_A Cuts at: 1548 3294 1548 Size: 1746 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4608 6079 136 Size: 107 122 71 631 605 2936 1471 1525 Fragments arranged by size: 2936 1525 1471 631 605 122 107 71 BglII A'GATC_T Cuts at: 3133 7463 3133 Size: 4330 3138 BlpI GC'TnA_GC Cuts at: 2258 4885 2258 Size: 2627 4841 Bme1580I G_kGCm'C Cuts at: 1790 2344 2468 2572 2627 2744 2877 3194 3575 Size: 554 124 104 55 117 133 317 381 Cuts at: 3575 4580 5529 6685 1790 Size: 1005 949 1156 2573 Fragments arranged by size: 2573 1156 1005 949 554 381 317 133 124 117 104 55 BmgBI CAC'GTC Cuts at: 1318 3189 1318 Size: 1871 5597 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5199 6157 442 Size: 416 53 83 399 24 3782 958 1753 Fragments arranged by size: 3782 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7468 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2260 4131 2260 Size: 1871 5597 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2255 2285 2660 3451 3935 4721 6128 Size: 875 141 30 375 791 484 786 1407 Cuts at: 6128 1239 Size: 2579 Fragments arranged by size: 2579 1407 875 791 786 484 375 141 30 Bpu10I CC'TnA_GC Cuts at: 2039 4598 2039 Size: 2559 4909 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2035 2918 3209 4013 4203 4262 5593 6458 6756 Size: 883 291 804 190 59 1331 865 298 Cuts at: 6756 2035 Size: 2747 Fragments arranged by size: 2747 1331 883 865 804 298 291 190 59 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4663 6131 882 Size: 617 3164 1468 2219 Fragments arranged by size: 3164 2219 1468 617 BsaAI yAC'GTr Cuts at: 493 1204 2587 5259 493 Size: 711 1383 2672 2702 Fragments arranged by size: 2702 2672 1383 711 BsaBI GATnn'nnATC Cuts at: 7462 7462 Size: 7468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2607 3491 3668 3680 Size: 53 83 186 936 1074 884 177 12 Cuts at: 3680 4485 5275 5657 6292 275 Size: 805 790 382 635 1451 Fragments arranged by size: 1451 1074 936 884 805 790 635 382 186 177 83 53 12 BsaWI w'CCGG_w Cuts at: 2085 3327 3700 5901 6299 6573 6720 7131 2085 Size: 1242 373 2201 398 274 147 411 2422 Fragments arranged by size: 2422 2201 1242 411 398 373 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3467 3497 3507 3537 3746 Size: 30 165 30 2064 30 10 30 209 Cuts at: 3746 3776 1178 Size: 30 4870 Fragments arranged by size: 4870 2064 209 165 30 30 30 30 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2788 3321 3472 3496 3824 3830 4466 Size: 1162 440 533 151 24 328 6 636 Cuts at: 4466 1186 Size: 4188 Fragments arranged by size: 4188 1162 636 533 440 328 151 24 6 BseYI C'CCAG_C Cuts at: 2187 2869 4647 6671 7167 2187 Size: 682 1778 2024 496 2488 Fragments arranged by size: 2488 2024 1778 682 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3377 1163 Size: 573 1641 5254 Fragments arranged by size: 5254 1641 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4918 5679 6707 1540 Size: 275 186 1966 951 761 1028 2301 Fragments arranged by size: 2301 1966 1028 951 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3194 3575 3930 5529 5614 6685 3194 Size: 381 355 1599 85 1071 3977 Fragments arranged by size: 3977 1599 1071 381 355 85 BsmI GAATG_Cn' Cuts at: 4979 5072 4979 Size: 93 7375 BsmBI CGTCTCn'nnnn_ Cuts at: 2432 3659 2432 Size: 1227 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2344 2468 2478 2513 2572 2627 2744 Size: 399 554 124 10 35 59 55 117 Cuts at: 2744 2877 3194 3575 3930 4112 4580 4787 5529 Size: 133 317 381 355 182 468 207 742 Cuts at: 5529 5614 6685 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 468 399 381 355 317 207 182 133 124 117 85 59 55 35 10 BspEI T'CCGG_A Cuts at: 2085 3327 7131 2085 Size: 1242 3804 2422 Fragments arranged by size: 3804 2422 1242 BspHI T'CATG_A Cuts at: 5357 7087 5357 Size: 1730 5738 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3399 3606 4823 844 Size: 2555 207 1217 3489 Fragments arranged by size: 3489 2555 1217 207 BsrBI CCG'CTC Cuts at: 1394 1823 3015 5355 1394 Size: 429 1192 2340 3507 Fragments arranged by size: 3507 2340 1192 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5963 6137 65 Size: 1183 4715 174 1396 Fragments arranged by size: 4715 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 3655 6112 6299 1700 Size: 110 311 1534 2457 187 2869 Fragments arranged by size: 2869 2457 1534 311 187 110 BsrGI T'GTAC_A Cuts at: 96 2577 96 Size: 2481 4987 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7468 BssSI C'ACGA_G Cuts at: 2858 5528 6540 2858 Size: 2670 1012 3786 Fragments arranged by size: 3786 2670 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7468 BstBI TT'CG_AA Cuts at: 6272 6272 Size: 7468 BstEII G'GTnAC_C Cuts at: 1184 3703 1184 Size: 2519 4949 BstXI CCAn_nnnn'nTGG Cuts at: 4690 4690 Size: 7468 BstYI r'GATC_y Cuts at: 1972 2487 2937 3120 3133 3310 4798 5165 5550 Size: 515 450 183 13 177 1488 367 385 Cuts at: 5550 5567 7019 7105 7117 7463 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1488 1452 515 450 385 367 346 183 177 86 17 13 12 Bsu36I CC'TnA_GG Cuts at: 2369 2369 Size: 7468 BtgI C'CryG_G Cuts at: 513 1065 2483 2601 3961 513 Size: 552 1418 118 1360 4020 Fragments arranged by size: 4020 1418 1360 552 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2652 487 Size: 683 56 1426 5303 Fragments arranged by size: 5303 1426 683 56 BtsI GCAGTG_nn' Cuts at: 4984 5778 5798 4984 Size: 794 20 6654 Fragments arranged by size: 6654 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7468 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7433 DraI TTT'AAA Cuts at: 4772 4772 Size: 7468 DraIII CAC_nnn'GTG Cuts at: 2484 2881 2484 Size: 397 7071 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6475 809 Size: 507 5159 1802 Fragments arranged by size: 5159 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 3964 3984 Size: 54 1425 50 275 18 421 1713 20 Cuts at: 3984 4122 4915 5804 7433 8 Size: 138 793 889 1629 43 Fragments arranged by size: 1713 1629 1425 889 793 421 275 138 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 4915 1537 Size: 275 2152 951 4090 Fragments arranged by size: 4090 2152 951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2909 3768 3789 4340 4646 4659 5398 1353 Size: 1556 859 21 551 306 13 739 3423 Fragments arranged by size: 3423 1556 859 739 551 306 21 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4418 6055 6429 6575 387 Size: 1064 138 2829 1637 374 146 1280 Fragments arranged by size: 2829 1637 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3928 3928 Size: 7468 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2114 2255 2285 2660 2792 3451 Size: 393 469 13 141 30 375 132 659 Cuts at: 3451 3869 3935 4321 4721 5531 6128 6915 7183 Size: 418 66 386 400 810 597 787 268 Cuts at: 7183 1239 Size: 1524 Fragments arranged by size: 1524 810 787 659 597 469 418 400 393 386 375 268 141 132 66 30 13 EcoNI CCTnn'n_nnAGG Cuts at: 2368 4142 4268 2368 Size: 1774 126 5568 Fragments arranged by size: 5568 1774 126 EcoO109I rG'GnC_Cy Cuts at: 2230 2269 2518 2568 2569 2784 4273 2230 Size: 39 249 50 1 215 1489 5425 Fragments arranged by size: 5425 1489 249 215 50 39 1 EcoRI G'AATT_C Cuts at: 4777 5155 4777 Size: 378 7090 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 3230 3961 4309 4483 2167 Size: 1063 731 348 174 5152 Fragments arranged by size: 5152 1063 731 348 174 FspI TGC'GCA Cuts at: 2222 4385 5974 2222 Size: 2163 1589 3716 Fragments arranged by size: 3716 2163 1589 HaeII r_GCGC'y Cuts at: 1678 2986 3007 3683 6615 1678 Size: 1308 21 676 2932 2531 Fragments arranged by size: 2932 2531 1308 676 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3697 3729 3746 3778 4140 Size: 32 135 32 2125 32 17 32 362 Cuts at: 4140 4172 4265 4297 6109 6141 6183 6215 1373 Size: 32 93 32 1812 32 42 32 2626 Fragments arranged by size: 2626 2125 1812 362 135 93 42 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 4198 4812 5058 669 Size: 988 281 2260 614 246 3079 Fragments arranged by size: 3079 2260 988 614 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7468 HpaI GTT'AAC Cuts at: 5058 5058 Size: 7468 KasI G'GCGC_C Cuts at: 3679 3679 Size: 7468 KpnI G_GTAC'C Cuts at: 4793 4793 Size: 7468 MfeI C'AATT_G Cuts at: 5067 5067 Size: 7468 MluI A'CGCG_T Cuts at: 7344 7344 Size: 7468 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 2085 3744 6582 6766 1305 Size: 744 36 1659 2838 184 2007 Fragments arranged by size: 2838 2007 1659 744 184 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 3986 4124 10 Size: 54 1425 343 2154 138 3354 Fragments arranged by size: 3354 2154 1425 343 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 2726 4583 4694 5426 5785 5944 Size: 648 1419 141 1857 111 732 359 159 Cuts at: 5944 518 Size: 2042 Fragments arranged by size: 2042 1857 1419 732 648 359 159 141 111 MspA1I CmG'CkG Cuts at: 1606 2128 2257 2680 2869 2958 2992 3913 3969 Size: 522 129 423 189 89 34 921 56 Cuts at: 3969 4435 4884 5561 6709 6954 1606 Size: 466 449 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 522 466 449 423 245 189 129 89 56 34 NaeI GCC'GGC Cuts at: 2123 2123 Size: 7468 NarI GG'CG_CC Cuts at: 3680 3680 Size: 7468 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6916 NdeI CA'TA_TG Cuts at: 387 387 Size: 7468 NgoMIV G'CCGG_C Cuts at: 2121 2121 Size: 7468 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7468 NotI GC'GGCC_GC Cuts at: 4915 4915 Size: 7468 NspI r_CATG'y Cuts at: 1132 1229 2386 4699 4826 6371 1132 Size: 97 1157 2313 127 1545 2229 Fragments arranged by size: 2313 2229 1545 1157 127 97 PasI CC'CwG_GG Cuts at: 1348 2514 1348 Size: 1166 6302 PciI A'CATG_T Cuts at: 2382 6367 2382 Size: 3985 3483 PflMI CCAn_nnn'nTGG Cuts at: 1108 2424 2484 3174 4530 1108 Size: 1316 60 690 1356 4046 Fragments arranged by size: 4046 1356 1316 690 60 PfoI T'CCnGG_A Cuts at: 2232 2232 Size: 7468 PmeI GTTT'AAAC Cuts at: 4772 4772 Size: 7468 PmlI CAC'GTG Cuts at: 2587 2587 Size: 7468 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 2989 5537 5569 7050 7082 2957 Size: 32 2548 32 1481 32 3343 Fragments arranged by size: 3343 2548 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2269 2518 2784 4273 2230 Size: 39 249 266 1489 5425 Fragments arranged by size: 5425 1489 266 249 39 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7468 PsiI TTA'TAA Cuts at: 5038 5038 Size: 7468 PspOMI G'GGCC_C Cuts at: 2568 2568 Size: 7468 PstI C_TGCA'G Cuts at: 830 4359 4762 4820 830 Size: 3529 403 58 3478 Fragments arranged by size: 3529 3478 403 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7468 PvuII CAG'CTG Cuts at: 2128 2257 2869 2958 3913 4435 2128 Size: 129 612 89 955 522 5161 Fragments arranged by size: 5161 955 612 522 129 89 RsrII CG'GwC_CG Cuts at: 2689 2689 Size: 7468 SacI G_AGCT'C Cuts at: 3930 3930 Size: 7468 SalI G'TCGA_C Cuts at: 1936 4810 1936 Size: 2874 4594 SanDI GG'GwC_CC Cuts at: 2518 2784 2518 Size: 266 7202 SapI GCTCTTCn'nnn_ Cuts at: 2909 2909 Size: 7468 SbfI CC_TGCA'GG Cuts at: 4820 4820 Size: 7468 ScaI AGT'ACT Cuts at: 2114 3173 5716 7428 2114 Size: 1059 2543 1712 2154 Fragments arranged by size: 2543 2154 1712 1059 SfcI C'TryA_G Cuts at: 826 1045 4057 4355 4758 4816 5951 6632 6823 Size: 219 3012 298 403 58 1135 681 191 Cuts at: 6823 826 Size: 1471 Fragments arranged by size: 3012 1471 1135 681 403 298 219 191 58 SfoI GGC'GCC Cuts at: 3681 3681 Size: 7468 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7468 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7468 SmaI CCC'GGG Cuts at: 1872 2566 2616 2781 2826 3677 4795 1872 Size: 694 50 165 45 851 1118 4545 Fragments arranged by size: 4545 1118 851 694 165 50 45 SmlI C'TyrA_G Cuts at: 820 1017 1943 2014 2933 3224 4028 4218 4277 Size: 197 926 71 919 291 804 190 59 Cuts at: 4277 5572 6473 6735 820 Size: 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 919 901 804 291 262 197 190 71 59 SnaBI TAC'GTA Cuts at: 493 5259 493 Size: 4766 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7468 SphI G_CATG'C Cuts at: 1132 4826 1132 Size: 3694 3774 SspI AAT'ATT Cuts at: 5 52 5392 5 Size: 47 5340 2081 Fragments arranged by size: 5340 2081 47 StuI AGG'CCT Cuts at: 1517 4034 1517 Size: 2517 4951 StyI C'CwwG_G Cuts at: 513 1065 4254 4495 4907 513 Size: 552 3189 241 412 3074 Fragments arranged by size: 3189 3074 552 412 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2510 2597 4085 5495 5512 5665 5850 Size: 1255 930 87 1488 1410 17 153 185 Cuts at: 5850 6295 325 Size: 445 1498 Fragments arranged by size: 1498 1488 1410 1255 930 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 2577 3171 Size: 274 80 33 51 1443 135 465 594 Cuts at: 3171 5714 7426 96 Size: 2543 1712 138 Fragments arranged by size: 2543 1712 1443 594 465 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2035 2067 3146 3178 3507 3539 7428 7460 2035 Size: 32 1079 32 329 32 3889 32 2043 Fragments arranged by size: 3889 2043 1079 329 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3334 1316 Size: 2018 5450 XbaI T'CTAG_A Cuts at: 4804 4804 Size: 7468 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4186 4186 Size: 7468 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7468 XmaI C'CCGG_G Cuts at: 1870 2564 2614 2779 2824 3675 4793 1870 Size: 694 50 165 45 851 1118 4545 Fragments arranged by size: 4545 1118 851 694 165 50 45 XmnI GAAnn'nnTTC Cuts at: 2114 3590 4292 5597 2114 Size: 1476 702 1305 3985 Fragments arranged by size: 3985 1476 1305 702 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3492 4486 5276 276 Size: 53 83 186 936 1958 994 790 2468 Fragments arranged by size: 2468 1958 994 936 790 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI SacII SexAI SfiI SrfI SwaI