(Linear) (Six-base) MAPSORT of: KIEE3325.seq Check: 2636 from: 1 to: 2763 KIEE3325.orf With 182 enzymes: SgfI * July 3, 2012 17:39 .. AatII G_ACGT'C Cuts at: 0 1489 2483 2763 Size: 1489 994 280 Fragments arranged by size: 1489 994 280 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 787 1864 2316 2763 Size: 96 691 1077 452 447 Fragments arranged by size: 1077 691 452 447 96 AfeI AGC'GCT Cuts at: 0 979 1000 2763 Size: 979 21 1763 Fragments arranged by size: 1763 979 21 AflIII A'CryG_T Cuts at: 0 377 2763 Size: 377 2386 AhdI GACnn_n'nnGTC Cuts at: 0 2274 2763 Size: 2274 489 AleI CACnn'nnGTG Cuts at: 0 580 721 2763 Size: 580 141 2042 Fragments arranged by size: 2042 580 141 AlwNI CAG_nnn'CTG Cuts at: 0 2135 2763 Size: 2135 628 ApaI G_GGCC'C Cuts at: 0 567 2763 Size: 567 2196 ApaLI G'TGCA_C Cuts at: 0 1185 1566 2763 Size: 1185 381 1197 Fragments arranged by size: 1197 1185 381 AvaI C'yCGr_G Cuts at: 0 559 609 774 819 1670 2763 Size: 559 50 165 45 851 1093 Fragments arranged by size: 1093 851 559 165 50 45 BamHI G'GATC_C Cuts at: 0 482 2763 Size: 482 2281 BanI G'GyrC_C Cuts at: 0 869 1647 1653 1674 2092 2409 2763 Size: 869 778 6 21 418 317 354 Fragments arranged by size: 869 778 418 354 317 21 6 BanII G_rGCy'C Cuts at: 0 473 508 567 1925 2107 2763 Size: 473 35 59 1358 182 656 Fragments arranged by size: 1358 656 473 182 59 35 BbeI G_GCGC'C Cuts at: 0 1678 2763 Size: 1678 1085 BbsI GAAGACnn'nnnn_ Cuts at: 0 2280 2763 Size: 2280 483 BbvCI CC'TCA_GC Cuts at: 0 2593 2763 Size: 2593 170 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1102 1136 2763 Size: 1102 34 1627 Fragments arranged by size: 1627 1102 34 BclI T'GATC_A Cuts at: 0 1289 2763 Size: 1289 1474 BglI GCCn_nnn'nGGC Cuts at: 0 2603 2763 Size: 2603 160 BglII A'GATC_T Cuts at: 0 1128 2763 Size: 1128 1635 BlpI GC'TnA_GC Cuts at: 0 253 2763 Size: 253 2510 Bme1580I G_kGCm'C Cuts at: 0 339 463 567 622 739 872 1189 1570 Size: 339 124 104 55 117 133 317 381 Cuts at: 1570 2575 2763 Size: 1005 188 Fragments arranged by size: 1005 381 339 317 188 133 124 117 104 55 BmgBI CAC'GTC Cuts at: 0 1184 2763 Size: 1184 1579 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 255 2126 2763 Size: 255 1871 637 Fragments arranged by size: 1871 637 255 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 250 280 655 1446 1930 2716 2763 Size: 109 141 30 375 791 484 786 47 Fragments arranged by size: 791 786 484 375 141 109 47 30 Bpu10I CC'TnA_GC Cuts at: 0 34 2593 2763 Size: 34 2559 170 Fragments arranged by size: 2559 170 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 913 1204 2008 2198 2257 2763 Size: 30 883 291 804 190 59 506 Fragments arranged by size: 883 804 506 291 190 59 30 BsaI GGTCTCn'nnnn_ Cuts at: 0 2658 2763 Size: 2658 105 BsaAI yAC'GTr Cuts at: 0 582 2763 Size: 582 2181 BsaHI Gr'CG_yC Cuts at: 0 602 1486 1663 1675 2480 2763 Size: 602 884 177 12 805 283 Fragments arranged by size: 884 805 602 283 177 12 BsaWI w'CCGG_w Cuts at: 0 80 1322 1695 2763 Size: 80 1242 373 1068 Fragments arranged by size: 1242 1068 373 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1462 1492 1502 1532 1741 1771 2763 Size: 1462 30 10 30 209 30 992 Fragments arranged by size: 1462 992 209 30 30 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 343 783 1316 1467 1491 1819 1825 2461 Size: 343 440 533 151 24 328 6 636 Cuts at: 2461 2763 Size: 302 Fragments arranged by size: 636 533 440 343 328 302 151 24 6 BseYI C'CCAG_C Cuts at: 0 182 864 2642 2763 Size: 182 682 1778 121 Fragments arranged by size: 1778 682 182 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1372 2763 Size: 1372 1391 BsiEI CG_ry'CG Cuts at: 0 1962 2763 Size: 1962 801 BsiHKAI G_wGCw'C Cuts at: 0 1189 1570 1925 2763 Size: 1189 381 355 838 Fragments arranged by size: 1189 838 381 355 BsmBI CGTCTCn'nnnn_ Cuts at: 0 427 1654 2763 Size: 427 1227 1109 Fragments arranged by size: 1227 1109 427 Bsp1286I G_dGCh'C Cuts at: 0 339 463 473 508 567 622 739 872 Size: 339 124 10 35 59 55 117 133 Cuts at: 872 1189 1570 1925 2107 2575 2763 Size: 317 381 355 182 468 188 Fragments arranged by size: 468 381 355 339 317 188 182 133 124 117 59 55 35 10 BspEI T'CCGG_A Cuts at: 0 80 1322 2763 Size: 80 1242 1441 Fragments arranged by size: 1441 1242 80 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1394 1601 2763 Size: 1394 207 1162 Fragments arranged by size: 1394 1162 207 BsrBI CCG'CTC Cuts at: 0 1010 2763 Size: 1010 1753 BsrFI r'CCGG_y Cuts at: 0 116 1650 2763 Size: 116 1534 1113 Fragments arranged by size: 1534 1113 116 BsrGI T'GTAC_A Cuts at: 0 572 2763 Size: 572 2191 BssSI C'ACGA_G Cuts at: 0 853 2763 Size: 853 1910 BstEII G'GTnAC_C Cuts at: 0 1698 2763 Size: 1698 1065 BstXI CCAn_nnnn'nTGG Cuts at: 0 2685 2763 Size: 2685 78 BstYI r'GATC_y Cuts at: 0 482 932 1115 1128 1305 2763 Size: 482 450 183 13 177 1458 Fragments arranged by size: 1458 482 450 183 177 13 Bsu36I CC'TnA_GG Cuts at: 0 364 2763 Size: 364 2399 BtgI C'CryG_G Cuts at: 0 478 596 1956 2763 Size: 478 118 1360 807 Fragments arranged by size: 1360 807 478 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 647 2763 Size: 647 2116 DraIII CAC_nnn'GTG Cuts at: 0 479 876 2763 Size: 479 397 1887 Fragments arranged by size: 1887 479 397 EaeI y'GGCC_r Cuts at: 0 246 1959 1979 2117 2763 Size: 246 1713 20 138 646 Fragments arranged by size: 1713 646 246 138 20 EagI C'GGCC_G Cuts at: 0 1959 2763 Size: 1959 804 EarI CTCTTCn'nnn_ Cuts at: 0 904 1763 1784 2335 2641 2654 2763 Size: 904 859 21 551 306 13 109 Fragments arranged by size: 904 859 551 306 109 21 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2413 2763 Size: 2413 350 EcoICRI GAG'CTC Cuts at: 0 1923 2763 Size: 1923 840 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 109 250 280 655 787 1446 1864 Size: 96 13 141 30 375 132 659 418 Cuts at: 1864 1930 2316 2716 2763 Size: 66 386 400 47 Fragments arranged by size: 659 418 400 386 375 141 132 96 66 47 30 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 363 2137 2263 2763 Size: 363 1774 126 500 Fragments arranged by size: 1774 500 363 126 EcoO109I rG'GnC_Cy Cuts at: 0 225 264 513 563 564 779 2268 2763 Size: 225 39 249 50 1 215 1489 495 Fragments arranged by size: 1489 495 249 225 215 50 39 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 162 1225 1956 2304 2478 2763 Size: 162 1063 731 348 174 285 Fragments arranged by size: 1063 731 348 285 174 162 FspI TGC'GCA Cuts at: 0 217 2380 2763 Size: 217 2163 383 Fragments arranged by size: 2163 383 217 HaeII r_GCGC'y Cuts at: 0 981 1002 1678 2763 Size: 981 21 676 1085 Fragments arranged by size: 1085 981 676 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1692 1724 1741 1773 2135 2167 2260 2292 Size: 1692 32 17 32 362 32 93 32 Cuts at: 2292 2763 Size: 471 Fragments arranged by size: 1692 471 362 93 32 32 32 32 17 HincII GTy'rAC Cuts at: 0 2193 2763 Size: 2193 570 KasI G'GCGC_C Cuts at: 0 1674 2763 Size: 1674 1089 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 44 80 1739 2763 Size: 44 36 1659 1024 Fragments arranged by size: 1659 1024 44 36 MscI TGG'CCA Cuts at: 0 1981 2119 2763 Size: 1981 138 644 Fragments arranged by size: 1981 644 138 MslI CAynn'nnrTG Cuts at: 0 580 721 2578 2689 2763 Size: 580 141 1857 111 74 Fragments arranged by size: 1857 580 141 111 74 MspA1I CmG'CkG Cuts at: 0 123 252 675 864 953 987 1908 1964 Size: 123 129 423 189 89 34 921 56 Cuts at: 1964 2430 2763 Size: 466 333 Fragments arranged by size: 921 466 423 333 189 129 123 89 56 34 NaeI GCC'GGC Cuts at: 0 118 2763 Size: 118 2645 NarI GG'CG_CC Cuts at: 0 1675 2763 Size: 1675 1088 NgoMIV G'CCGG_C Cuts at: 0 116 2763 Size: 116 2647 NspI r_CATG'y Cuts at: 0 381 2694 2763 Size: 381 2313 69 Fragments arranged by size: 2313 381 69 PasI CC'CwG_GG Cuts at: 0 509 2763 Size: 509 2254 PciI A'CATG_T Cuts at: 0 377 2763 Size: 377 2386 PflMI CCAn_nnn'nTGG Cuts at: 0 419 479 1169 2525 2763 Size: 419 60 690 1356 238 Fragments arranged by size: 1356 690 419 238 60 PfoI T'CCnGG_A Cuts at: 0 227 2763 Size: 227 2536 PmlI CAC'GTG Cuts at: 0 582 2763 Size: 582 2181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 952 984 2763 Size: 952 32 1779 Fragments arranged by size: 1779 952 32 PpuMI rG'GwC_Cy Cuts at: 0 225 264 513 779 2268 2763 Size: 225 39 249 266 1489 495 Fragments arranged by size: 1489 495 266 249 225 39 PspOMI G'GGCC_C Cuts at: 0 563 2763 Size: 563 2200 PstI C_TGCA'G Cuts at: 0 2354 2757 2763 Size: 2354 403 6 Fragments arranged by size: 2354 403 6 PvuII CAG'CTG Cuts at: 0 123 252 864 953 1908 2430 2763 Size: 123 129 612 89 955 522 333 Fragments arranged by size: 955 612 522 333 129 123 89 RsrII CG'GwC_CG Cuts at: 0 684 2763 Size: 684 2079 SacI G_AGCT'C Cuts at: 0 1925 2763 Size: 1925 838 SanDI GG'GwC_CC Cuts at: 0 513 779 2763 Size: 513 266 1984 Fragments arranged by size: 1984 513 266 SapI GCTCTTCn'nnn_ Cuts at: 0 904 2763 Size: 904 1859 ScaI AGT'ACT Cuts at: 0 109 1168 2763 Size: 109 1059 1595 Fragments arranged by size: 1595 1059 109 SfcI C'TryA_G Cuts at: 0 2052 2350 2753 2763 Size: 2052 298 403 10 Fragments arranged by size: 2052 403 298 10 SfoI GGC'GCC Cuts at: 0 1676 2763 Size: 1676 1087 SmaI CCC'GGG Cuts at: 0 561 611 776 821 1672 2763 Size: 561 50 165 45 851 1091 Fragments arranged by size: 1091 851 561 165 50 45 SmlI C'TyrA_G Cuts at: 0 9 928 1219 2023 2213 2272 2763 Size: 9 919 291 804 190 59 491 Fragments arranged by size: 919 804 491 291 190 59 9 StuI AGG'CCT Cuts at: 0 2029 2763 Size: 2029 734 StyI C'CwwG_G Cuts at: 0 2249 2490 2763 Size: 2249 241 273 Fragments arranged by size: 2249 273 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 505 592 2080 2763 Size: 505 87 1488 683 Fragments arranged by size: 1488 683 505 87 TatI w'GTAC_w Cuts at: 0 107 572 1166 2763 Size: 107 465 594 1597 Fragments arranged by size: 1597 594 465 107 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 30 62 1141 1173 1502 1534 2763 Size: 30 32 1079 32 329 32 1229 Fragments arranged by size: 1229 1079 329 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 0 1329 2763 Size: 1329 1434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2181 2763 Size: 2181 582 XmaI C'CCGG_G Cuts at: 0 559 609 774 819 1670 2763 Size: 559 50 165 45 851 1093 Fragments arranged by size: 1093 851 559 165 50 45 XmnI GAAnn'nnTTC Cuts at: 0 109 1585 2287 2763 Size: 109 1476 702 476 Fragments arranged by size: 1476 702 476 109 ZraI GAC'GTC Cuts at: 0 1487 2481 2763 Size: 1487 994 282 Fragments arranged by size: 1487 994 282 Enzymes that do cut: AatII AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SanDI SapI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BmrI BmtI BsaBI BsiWI BsmI BspHI BsrDI BssHII BstAPI BstBI BstZ17I BtsI ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI SacII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI