################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrDI BssHII BssSI BstBI BstEII BstPI BstUI BstXI CfoI ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI MspA1I MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI Sse8387I SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 2325 GTATAC GT^[AC][GT]AC AocI 765 CCTGAGG CC^T[ACGT]AGG AseI 1975 ATTAAT AT^TAAT AsnI 1975 ATTAAT AT^TAAT AspI 1291 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 458 CCCGAG C^[CT]CG[GA]G AvrII 447 CCTAGG C^CTAGG BalI 596 TGGCCA TGG^CCA BcnI 2043 CCCGG CC^[GC]GG BlnI 447 CCTAGG C^CTAGG BlpI 723 GCTGAGC GC^T[ACGT]AGC BpmI 887 CTGGAG CTGGAG(16/14) Bpu1102I 723 GCTGAGC GC^T[ACGT]AGC BsaI 1006 GGTCTC GGTCTC(1/5) BsmBI 1775 CGTCTC CGTCTC(1/5) BsoBI 458 CCCGAG C^[CT]CG[GA]G Bsp1407I 336 TGTACA T^GTACA BspHI 906 TCATGA T^CATGA BsrBI 136 GAGCGG CCGCTC(-3/-3) BsrFI 74 ACCGGC [GA]^CCGG[CT] BsrGI 336 TGTACA T^GTACA Bst1107I 2325 GTATAC GTA^TAC BstAPI 300 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2325 GTATAC GTA^TAC Bsu36I 765 CCTGAGG CC^T[ACGT]AGG CelII 723 GCTGAGC GC^T[ACGT]AGC Cfr10I 74 ACCGGC [GA]^CCGG[CT] DraI 2143 TTTAAA TTT^AAA DrdI 1849 GACTGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 347 CCATGG C^C[GA][CT]GG Eco81I 765 CCTGAGG CC^T[ACGT]AGG EcoNI 1111 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG MfeI 1990 CAATTG C^AATTG MluNI 596 TGGCCA TGG^CCA MscI 596 TGGCCA TGG^CCA MunI 1990 CAATTG C^AATTG NciI 2043 CCCGG CC^[GC]GG NcoI 347 CCATGG C^CATGG NheI 487 GCTAGC G^CTAGC NspI 609 GCATGC [GA]CATG^[CT] PflFI 1291 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 443 GGGACCT [GA]G^G[AT]CC[CT] PshBI 1975 ATTAAT AT^TAAT RcaI 906 TCATGA T^CATGA SgrAI 73 CACCGGCG C[GA]^CCGG[CT]G SpeI 2193 ACTAGT A^CTAGT SphI 609 GCATGC GCATG^C SspBI 336 TGTACA T^GTACA SspI 1096 AATATT AAT^ATT StuI 733 AGGCCT AGG^CCT Tth111I 1291 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 1368 GGATC GGATC(4/5) 1689 GGATC AspHI 1495 GAGCAC G[AT]GC[AT]^C 2308 GAGCAC BbsI 1813 GAAGAC GAAGAC(2/6) 409 GTCTTC BglII 390 AGATCT A^GATCT 1527 AGATCT BpuAI 1813 GAAGAC GAAGAC(2/6) 409 GTCTTC BsiHKAI 1495 GAGCAC G[AT]GC[AT]^C 2308 GAGCAC BstYI 390 AGATCT [GA]^GATC[CT] 1527 AGATCT EaeI 8 TGGCCG [CT]^GGCC[GA] 596 TGGCCA EcoRI 633 GAATTC G^AATTC 2065 GAATTC HapII 75 CCGG C^CGG 2044 CCGG HpaII 75 CCGG C^CGG 2044 CCGG MaeII 1629 ACGT A^CGT 2298 ACGT MflI 390 AGATCT [GA]^GATC[CT] 1527 AGATCT MslI 240 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2311 CACATGGGTG MspI 75 CCGG C^CGG 2044 CCGG NlaIV 443 GGGACC GG[ACGT]^[ACGT]CC 943 GGCTCC XhoII 390 AGATCT [GA]^GATC[CT] 1527 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AvaII BanII BbvI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I