(Circular) (Six-base) MAPSORT of: pFN21AE3312.seq Check: 8986 from: 1 to: 7090 pFN21AE3312 7090 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3034 4246 3034 Size: 1212 5878 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4900 278 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4331 4433 1937 Size: 2394 102 4594 Fragments arranged by size: 4594 2394 102 Acc65I G'GTAC_C Cuts at: 4411 4411 Size: 7090 AclI AA'CG_TT Cuts at: 1563 5217 5590 1563 Size: 3654 373 3063 Fragments arranged by size: 3654 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2763 3741 3750 4146 5153 6537 6805 1632 Size: 1131 978 9 396 1007 1384 268 1917 Fragments arranged by size: 1917 1384 1131 1007 978 396 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6893 AflIII A'CryG_T Cuts at: 1203 5989 6966 1203 Size: 4786 977 1327 Fragments arranged by size: 4786 1327 977 AgeI A'CCGG_T Cuts at: 5921 5921 Size: 7090 AhdI GACnn_n'nnGTC Cuts at: 5819 5819 Size: 7090 AleI CACnn'nnGTG Cuts at: 1166 4320 1166 Size: 3154 3936 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7090 AlwNI CAG_nnn'CTG Cuts at: 2311 4012 4181 4833 6405 2311 Size: 1701 169 652 1572 2996 Fragments arranged by size: 2996 1701 1572 652 169 ApaLI G'TGCA_C Cuts at: 5147 6303 5147 Size: 1156 5934 ApoI r'AATT_y Cuts at: 1493 2638 4070 4286 4364 4384 4399 4625 4777 Size: 1145 1432 216 78 20 15 226 152 Cuts at: 4777 6886 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1432 1145 226 216 152 78 20 15 AseI AT'TA_AT Cuts at: 160 1032 3981 5644 160 Size: 872 2949 1663 1606 Fragments arranged by size: 2949 1663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 AvaI C'yCGr_G Cuts at: 1870 1943 2463 4415 1870 Size: 73 520 1952 4545 Fragments arranged by size: 4545 1952 520 73 AvrII C'CTAG_G Cuts at: 2452 2452 Size: 7090 BamHI G'GATC_C Cuts at: 4420 4787 4420 Size: 367 6723 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4411 5866 618 Size: 325 217 627 2624 1455 1842 Fragments arranged by size: 2624 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2528 2578 2951 4409 1391 Size: 1137 50 373 1458 4072 Fragments arranged by size: 4072 1458 1137 373 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2407 3825 928 Size: 971 508 1418 4193 Fragments arranged by size: 4193 1418 971 508 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5281 5315 1903 Size: 34 50 34 3260 34 3678 Fragments arranged by size: 3678 3260 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4982 6192 4982 Size: 1210 5880 BclI T'GATC_A Cuts at: 1548 1548 Size: 7090 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5701 136 Size: 107 122 71 631 605 4029 1525 Fragments arranged by size: 4029 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2395 3532 7085 2395 Size: 1137 3553 2400 Fragments arranged by size: 3553 2400 1137 BlpI GC'TnA_GC Cuts at: 2729 4507 2729 Size: 1778 5312 Bme1580I G_kGCm'C Cuts at: 1790 3262 5151 6307 1790 Size: 1472 1889 1156 2573 Fragments arranged by size: 2573 1889 1472 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3315 3657 4055 Size: 416 53 83 399 24 1898 342 398 Cuts at: 4055 4821 5779 442 Size: 766 958 1753 Fragments arranged by size: 1898 1753 958 766 416 399 398 342 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2496 1056 Size: 1440 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2913 5750 1239 Size: 1674 2837 2579 Fragments arranged by size: 2837 2579 1674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2273 3082 3201 4231 5215 6080 6378 2273 Size: 809 119 1030 984 865 298 2985 Fragments arranged by size: 2985 1030 984 865 809 298 119 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3017 5753 882 Size: 617 1518 2736 2219 Fragments arranged by size: 2736 2219 1518 617 BsaAI yAC'GTr Cuts at: 493 1204 4881 493 Size: 711 3677 2702 Fragments arranged by size: 3677 2702 711 BsaBI GATnn'nnATC Cuts at: 7084 7084 Size: 7090 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4897 5279 5914 275 Size: 53 83 186 936 3364 382 635 1451 Fragments arranged by size: 3364 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5523 5921 6195 6342 6753 5523 Size: 398 274 147 411 5860 Fragments arranged by size: 5860 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2103 2133 1178 Size: 30 165 30 700 30 6135 Fragments arranged by size: 6135 700 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2241 2776 3242 3499 3549 3733 3838 4289 Size: 1055 535 466 257 50 184 105 451 Cuts at: 4289 4292 1186 Size: 3 3984 Fragments arranged by size: 3984 1055 535 466 451 257 184 105 50 3 BseYI C'CCAG_C Cuts at: 2166 3261 6293 6789 2166 Size: 1095 3032 496 2467 Fragments arranged by size: 3032 2467 1095 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6517 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4540 5301 6329 1540 Size: 275 186 2539 761 1028 2301 Fragments arranged by size: 2539 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3504 4317 5151 5236 6307 3504 Size: 813 834 85 1071 4287 Fragments arranged by size: 4287 1071 834 813 85 BsmI GAATG_Cn' Cuts at: 4601 4694 4601 Size: 93 6997 BsmBI CGTCTCn'nnnn_ Cuts at: 3786 3786 Size: 7090 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2528 2578 2951 3262 3504 4317 4409 Size: 399 738 50 373 311 242 813 92 Cuts at: 4409 5151 5236 6307 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 813 742 738 399 373 311 242 92 85 50 BspEI T'CCGG_A Cuts at: 6753 6753 Size: 7090 BspHI T'CATG_A Cuts at: 2911 4979 6709 2911 Size: 2068 1730 3292 Fragments arranged by size: 3292 2068 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 3034 3056 3124 4246 4445 844 Size: 1193 997 22 68 1122 199 3489 Fragments arranged by size: 3489 1193 1122 997 199 68 22 BsrBI CCG'CTC Cuts at: 1394 1823 2143 4977 1394 Size: 429 320 2834 3507 Fragments arranged by size: 3507 2834 429 320 BsrDI GCAATG_nn' Cuts at: 65 1248 5585 5759 65 Size: 1183 4337 174 1396 Fragments arranged by size: 4337 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2079 5734 5921 1700 Size: 110 269 3655 187 2869 Fragments arranged by size: 3655 2869 269 187 110 BsrGI T'GTAC_A Cuts at: 96 2341 96 Size: 2245 4845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7090 BssSI C'ACGA_G Cuts at: 5150 6162 5150 Size: 1012 6078 BstAPI GCAn_nnn'nTGC Cuts at: 794 2311 794 Size: 1517 5573 BstBI TT'CG_AA Cuts at: 5894 5894 Size: 7090 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7090 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 7090 BstYI r'GATC_y Cuts at: 1972 2395 3532 4420 4787 5172 5189 6641 6727 Size: 423 1137 888 367 385 17 1452 86 Cuts at: 6727 6739 7085 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1137 888 423 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4332 4332 Size: 7090 Bsu36I CC'TnA_GG Cuts at: 2771 2771 Size: 7090 BtgI C'CryG_G Cuts at: 513 1065 2352 513 Size: 552 1287 5251 Fragments arranged by size: 5251 1287 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6351 Fragments arranged by size: 6351 683 56 BtsI GCAGTG_nn' Cuts at: 3679 4606 5400 5420 3679 Size: 927 794 20 5349 Fragments arranged by size: 5349 927 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7090 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7055 DraI TTT'AAA Cuts at: 4150 4394 4150 Size: 244 6846 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3860 6097 809 Size: 507 2544 2237 1802 Fragments arranged by size: 2544 2237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2601 4537 Size: 54 1425 50 275 18 183 588 1936 Cuts at: 4537 5426 7055 8 Size: 889 1629 43 Fragments arranged by size: 1936 1629 1425 889 588 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4537 1537 Size: 275 2725 4090 Fragments arranged by size: 4090 2725 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2944 3219 3760 4122 5020 1353 Size: 1591 275 541 362 898 3423 Fragments arranged by size: 3423 1591 898 541 362 275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5677 6051 6197 387 Size: 1064 138 4088 374 146 1280 Fragments arranged by size: 4088 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2763 2913 3741 3750 4146 5153 5750 Size: 393 1131 150 828 9 396 1007 597 Cuts at: 5750 6537 6805 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1131 1007 828 787 597 396 393 268 150 9 EcoNI CCTnn'n_nnAGG Cuts at: 3120 3120 Size: 7090 EcoO109I rG'GnC_Cy Cuts at: 2449 3003 3761 2449 Size: 554 758 5778 Fragments arranged by size: 5778 758 554 EcoRI G'AATT_C Cuts at: 2638 4070 4399 4777 2638 Size: 1432 329 378 4951 Fragments arranged by size: 4951 1432 378 329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2324 2324 Size: 7090 FspI TGC'GCA Cuts at: 5596 5596 Size: 7090 HaeII r_GCGC'y Cuts at: 1678 6237 1678 Size: 4559 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2574 2606 2849 2881 2888 Size: 32 135 32 1002 32 243 32 7 Cuts at: 2888 2920 3590 3622 3668 3700 3874 3906 5731 Size: 32 670 32 46 32 174 32 1825 Cuts at: 5731 5763 5805 5837 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1825 1002 670 243 174 135 46 42 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 4434 4680 669 Size: 988 281 2496 246 3079 Fragments arranged by size: 3079 2496 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7090 HpaI GTT'AAC Cuts at: 4680 4680 Size: 7090 KpnI G_GTAC'C Cuts at: 4415 4415 Size: 7090 MfeI C'AATT_G Cuts at: 3995 4689 3995 Size: 694 6396 MluI A'CGCG_T Cuts at: 6966 6966 Size: 7090 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6204 6388 1305 Size: 4899 184 2007 Fragments arranged by size: 4899 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2603 10 Size: 54 1425 343 771 4497 Fragments arranged by size: 4497 1425 771 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 4320 5048 5407 5566 518 Size: 648 1083 2071 728 359 159 2042 Fragments arranged by size: 2071 2042 1083 728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4506 5183 6331 6576 1606 Size: 2900 677 1148 245 2120 Fragments arranged by size: 2900 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2352 513 Size: 552 1287 5251 Fragments arranged by size: 5251 1287 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7090 NheI G'CTAG_C Cuts at: 1052 2492 1052 Size: 1440 5650 NotI GC'GGCC_GC Cuts at: 4537 4537 Size: 7090 NspI r_CATG'y Cuts at: 1132 1229 2618 4448 5993 1132 Size: 97 1389 1830 1545 2229 Fragments arranged by size: 2229 1830 1545 1389 97 PasI CC'CwG_GG Cuts at: 1348 3000 1348 Size: 1652 5438 PciI A'CATG_T Cuts at: 5989 5989 Size: 7090 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7090 PmeI GTTT'AAAC Cuts at: 4394 4394 Size: 7090 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5159 5191 6672 6704 5159 Size: 32 1481 32 5545 Fragments arranged by size: 5545 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 2449 Size: 7090 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7090 PsiI TTA'TAA Cuts at: 4660 4660 Size: 7090 PstI C_TGCA'G Cuts at: 830 3240 3339 3860 4442 830 Size: 2410 99 521 582 3478 Fragments arranged by size: 3478 2410 582 521 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7090 SalI G'TCGA_C Cuts at: 1936 4432 1936 Size: 2496 4594 SbfI CC_TGCA'GG Cuts at: 4442 4442 Size: 7090 ScaI AGT'ACT Cuts at: 5338 7050 5338 Size: 1712 5378 SfcI C'TryA_G Cuts at: 826 1045 2880 3236 3335 3856 4438 5573 6254 Size: 219 1835 356 99 521 582 1135 681 Cuts at: 6254 6445 826 Size: 191 1471 Fragments arranged by size: 1835 1471 1135 681 582 521 356 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 SgrAI Cr'CCGG_yG Cuts at: 1700 2079 1700 Size: 379 6711 SmaI CCC'GGG Cuts at: 1872 4417 1872 Size: 2545 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 3061 3216 4210 5194 6095 Size: 197 926 345 773 155 994 984 901 Cuts at: 6095 6357 820 Size: 262 1553 Fragments arranged by size: 1553 994 984 926 901 773 345 262 197 155 SnaBI TAC'GTA Cuts at: 493 4881 493 Size: 4388 2702 SpeI A'CTAG_T Cuts at: 152 4198 152 Size: 4046 3044 SphI G_CATG'C Cuts at: 1132 2618 4448 1132 Size: 1486 1830 3774 Fragments arranged by size: 3774 1830 1486 SspI AAT'ATT Cuts at: 5 52 3103 5014 5 Size: 47 3051 1911 2081 Fragments arranged by size: 3051 2081 1911 47 StuI AGG'CCT Cuts at: 1517 2740 1517 Size: 1223 5867 StyI C'CwwG_G Cuts at: 513 1065 2352 2452 3987 4529 513 Size: 552 1287 100 1535 542 3074 Fragments arranged by size: 3074 1535 1287 552 542 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2957 3435 4310 5117 5134 5287 5472 Size: 1255 1377 478 875 807 17 153 185 Cuts at: 5472 5917 325 Size: 445 1498 Fragments arranged by size: 1498 1377 1255 875 807 478 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2341 5336 7048 Size: 274 80 33 51 1443 364 2995 1712 Cuts at: 7048 96 Size: 138 Fragments arranged by size: 2995 1712 1443 364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7050 7082 7050 Size: 32 7058 Tth111I GACn'n_nGTC Cuts at: 1316 3299 1316 Size: 1983 5107 XbaI T'CTAG_A Cuts at: 4426 4426 Size: 7090 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7090 XmaI C'CCGG_G Cuts at: 1870 4415 1870 Size: 2545 4545 XmnI GAAnn'nnTTC Cuts at: 5219 5219 Size: 7090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4898 276 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI