(Linear) (Six-base) MAPSORT of: KIEE3312.seq Check: 7269 from: 1 to: 2385 KIEE3312.orf With 182 enzymes: SgfI * July 3, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1029 2241 2385 Size: 1029 1212 144 Fragments arranged by size: 1212 1029 144 AccI GT'mk_AC Cuts at: 0 2326 2385 Size: 2326 59 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 758 1736 1745 2141 2385 Size: 758 978 9 396 244 Fragments arranged by size: 978 758 396 244 9 AleI CACnn'nnGTG Cuts at: 0 2315 2385 Size: 2315 70 AlwNI CAG_nnn'CTG Cuts at: 0 306 2007 2176 2385 Size: 306 1701 169 209 Fragments arranged by size: 1701 306 209 169 ApoI r'AATT_y Cuts at: 0 633 2065 2281 2359 2379 2385 Size: 633 1432 216 78 20 6 Fragments arranged by size: 1432 633 216 78 20 6 AseI AT'TA_AT Cuts at: 0 1976 2385 Size: 1976 409 AvaI C'yCGr_G Cuts at: 0 458 2385 Size: 458 1927 AvrII C'CTAG_G Cuts at: 0 447 2385 Size: 447 1938 BanII G_rGCy'C Cuts at: 0 523 573 946 2385 Size: 523 50 373 1439 Fragments arranged by size: 1439 523 373 50 BbsI GAAGACnn'nnnn_ Cuts at: 0 402 1820 2385 Size: 402 1418 565 Fragments arranged by size: 1418 565 402 BglII A'GATC_T Cuts at: 0 390 1527 2385 Size: 390 1137 858 Fragments arranged by size: 1137 858 390 BlpI GC'TnA_GC Cuts at: 0 724 2385 Size: 724 1661 Bme1580I G_kGCm'C Cuts at: 0 1257 2385 Size: 1257 1128 BmrI ACTGGGnnnn_n' Cuts at: 0 1310 1652 2050 2385 Size: 1310 342 398 335 Fragments arranged by size: 1310 398 342 335 BmtI G_CTAG'C Cuts at: 0 491 2385 Size: 491 1894 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 908 2385 Size: 908 1477 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 1077 1196 2226 2385 Size: 268 809 119 1030 159 Fragments arranged by size: 1030 809 268 159 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 1012 2385 Size: 1012 1373 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 98 128 2385 Size: 98 30 2257 Fragments arranged by size: 2257 98 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 236 771 1237 1494 1544 1728 1833 2284 Size: 236 535 466 257 50 184 105 451 Cuts at: 2284 2287 2385 Size: 3 98 Fragments arranged by size: 535 466 451 257 236 184 105 98 50 3 BseYI C'CCAG_C Cuts at: 0 161 1256 2385 Size: 161 1095 1129 Fragments arranged by size: 1129 1095 161 BsiHKAI G_wGCw'C Cuts at: 0 1499 2312 2385 Size: 1499 813 73 Fragments arranged by size: 1499 813 73 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1781 2385 Size: 1781 604 Bsp1286I G_dGCh'C Cuts at: 0 523 573 946 1257 1499 2312 2385 Size: 523 50 373 311 242 813 73 Fragments arranged by size: 813 523 373 311 242 73 50 BspHI T'CATG_A Cuts at: 0 906 2385 Size: 906 1479 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 32 1029 1051 1119 2241 2385 Size: 32 997 22 68 1122 144 Fragments arranged by size: 1122 997 144 68 32 22 BsrBI CCG'CTC Cuts at: 0 138 2385 Size: 138 2247 BsrFI r'CCGG_y Cuts at: 0 74 2385 Size: 74 2311 BsrGI T'GTAC_A Cuts at: 0 336 2385 Size: 336 2049 BstAPI GCAn_nnn'nTGC Cuts at: 0 306 2385 Size: 306 2079 BstYI r'GATC_y Cuts at: 0 390 1527 2385 Size: 390 1137 858 Fragments arranged by size: 1137 858 390 BstZ17I GTA'TAC Cuts at: 0 2327 2385 Size: 2327 58 Bsu36I CC'TnA_GG Cuts at: 0 766 2385 Size: 766 1619 BtgI C'CryG_G Cuts at: 0 347 2385 Size: 347 2038 BtsI GCAGTG_nn' Cuts at: 0 1674 2385 Size: 1674 711 DraI TTT'AAA Cuts at: 0 2145 2385 Size: 2145 240 DrdI GACnn_nn'nnGTC Cuts at: 0 1855 2385 Size: 1855 530 EaeI y'GGCC_r Cuts at: 0 8 596 2385 Size: 8 588 1789 Fragments arranged by size: 1789 588 8 EarI CTCTTCn'nnn_ Cuts at: 0 939 1214 1755 2117 2385 Size: 939 275 541 362 268 Fragments arranged by size: 939 541 362 275 268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 758 908 1736 1745 2141 2385 Size: 758 150 828 9 396 244 Fragments arranged by size: 828 758 396 244 150 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 1115 2385 Size: 1115 1270 EcoO109I rG'GnC_Cy Cuts at: 0 444 998 1756 2385 Size: 444 554 758 629 Fragments arranged by size: 758 629 554 444 EcoRI G'AATT_C Cuts at: 0 633 2065 2385 Size: 633 1432 320 Fragments arranged by size: 1432 633 320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 319 2385 Size: 319 2066 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 569 601 844 876 883 915 1585 1617 Size: 569 32 243 32 7 32 670 32 Cuts at: 1617 1663 1695 1869 1901 2385 Size: 46 32 174 32 484 Fragments arranged by size: 670 569 484 243 174 46 32 32 32 32 32 32 7 MfeI C'AATT_G Cuts at: 0 1990 2385 Size: 1990 395 MscI TGG'CCA Cuts at: 0 598 2385 Size: 598 1787 MslI CAynn'nnrTG Cuts at: 0 244 2315 2385 Size: 244 2071 70 Fragments arranged by size: 2071 244 70 NcoI C'CATG_G Cuts at: 0 347 2385 Size: 347 2038 NheI G'CTAG_C Cuts at: 0 487 2385 Size: 487 1898 NspI r_CATG'y Cuts at: 0 613 2385 Size: 613 1772 PasI CC'CwG_GG Cuts at: 0 995 2385 Size: 995 1390 PpuMI rG'GwC_Cy Cuts at: 0 444 2385 Size: 444 1941 PstI C_TGCA'G Cuts at: 0 1235 1334 1855 2385 Size: 1235 99 521 530 Fragments arranged by size: 1235 530 521 99 SfcI C'TryA_G Cuts at: 0 875 1231 1330 1851 2385 Size: 875 356 99 521 534 Fragments arranged by size: 875 534 521 356 99 SgrAI Cr'CCGG_yG Cuts at: 0 74 2385 Size: 74 2311 SmlI C'TyrA_G Cuts at: 0 283 1056 1211 2205 2385 Size: 283 773 155 994 180 Fragments arranged by size: 994 773 283 180 155 SpeI A'CTAG_T Cuts at: 0 2193 2385 Size: 2193 192 SphI G_CATG'C Cuts at: 0 613 2385 Size: 613 1772 SspI AAT'ATT Cuts at: 0 1098 2385 Size: 1098 1287 StuI AGG'CCT Cuts at: 0 735 2385 Size: 735 1650 StyI C'CwwG_G Cuts at: 0 347 447 1982 2385 Size: 347 100 1535 403 Fragments arranged by size: 1535 403 347 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 952 1430 2305 2385 Size: 952 478 875 80 Fragments arranged by size: 952 875 478 80 TatI w'GTAC_w Cuts at: 0 336 2385 Size: 336 2049 Tth111I GACn'n_nGTC Cuts at: 0 1294 2385 Size: 1294 1091 Enzymes that do cut: AarI AccI AcuI AleI AlwNI ApoI AseI AvaI AvrII BanII BbsI BglII BlpI Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MfeI MscI MslI NcoI NheI NspI PasI PpuMI PstI SfcI SgrAI SmlI SpeI SphI SspI StuI StyI TaqII TatI Tth111I Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BsrDI BssHII BssSI BstBI BstEII BstXI BtgZI ClaI CspCI DraIII EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SrfI SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI