(Circular) (Six-base) MAPSORT of: pFN21AB9900.seq Check: 7643 from: 1 to: 5827 pFN21AB9900 5827 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 2955 3090 3148 2955 Size: 135 58 5634 Fragments arranged by size: 5634 135 58 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3019 3890 5274 5542 1632 Size: 1387 871 1384 268 1917 Fragments arranged by size: 1917 1387 1384 871 268 AfeI AGC'GCT Cuts at: 2435 2435 Size: 5827 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 2703 4726 5703 1203 Size: 1500 2023 977 1327 Fragments arranged by size: 2023 1500 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 2267 1166 Size: 1101 4726 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 2038 2077 2469 2680 2968 3570 5142 2038 Size: 39 392 211 288 602 1572 2723 Fragments arranged by size: 2723 1572 602 392 288 211 39 ApaI G_GGCC'C Cuts at: 2021 2870 2021 Size: 849 4978 ApaLI G'TGCA_C Cuts at: 2366 3884 5040 2366 Size: 1518 1156 3153 Fragments arranged by size: 3153 1518 1156 ApoI r'AATT_y Cuts at: 1493 3136 3362 3514 5623 1493 Size: 1643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2115 3152 1870 Size: 73 172 1037 4545 Fragments arranged by size: 4545 1037 172 73 AvrII C'CTAG_G Cuts at: 2138 2138 Size: 5827 BamHI G'GATC_C Cuts at: 2008 2132 3157 3524 2008 Size: 124 1025 367 4311 Fragments arranged by size: 4311 1025 367 124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2043 2296 2475 2955 3090 Size: 325 217 627 256 253 179 480 135 Cuts at: 3090 3148 4603 618 Size: 58 1455 1842 Fragments arranged by size: 1842 1455 627 480 325 256 253 217 179 135 58 BanII G_rGCy'C Cuts at: 1391 2021 2179 2870 2939 3146 1391 Size: 630 158 691 69 207 4072 Fragments arranged by size: 4072 691 630 207 158 69 BbeI G_GCGC'C Cuts at: 2047 2047 Size: 5827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4856 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4018 4052 1903 Size: 34 2081 34 3678 Fragments arranged by size: 3678 2081 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2489 3719 4929 2489 Size: 1230 1210 3387 Fragments arranged by size: 3387 1230 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 2428 2840 Size: 107 122 71 631 605 363 393 412 Cuts at: 2840 4438 136 Size: 1598 1525 Fragments arranged by size: 1598 1525 631 605 412 393 363 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2021 2301 2370 2856 2870 3046 3888 5044 Size: 231 280 69 486 14 176 842 1156 Cuts at: 5044 1790 Size: 2573 Fragments arranged by size: 2573 1156 842 486 280 231 176 69 14 BmgBI CAC'GTC Cuts at: 1318 2250 2704 1318 Size: 932 454 4441 Fragments arranged by size: 4441 932 454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 2388 3558 Size: 416 53 83 399 24 966 5 1170 Cuts at: 3558 4516 442 Size: 958 1753 Fragments arranged by size: 1753 1170 966 958 416 399 83 53 24 5 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2317 2579 2858 4487 1239 Size: 1078 262 279 1629 2579 Fragments arranged by size: 2579 1629 1078 279 262 Bpu10I CC'TnA_GC Cuts at: 2993 2993 Size: 5827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2678 3952 4817 5115 2678 Size: 1274 865 298 3390 Fragments arranged by size: 3390 1274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2920 3618 493 Size: 711 1716 698 2702 Fragments arranged by size: 2702 1716 711 698 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2044 2907 3634 4016 Size: 53 83 186 936 511 863 727 382 Cuts at: 4016 4651 275 Size: 635 1451 Fragments arranged by size: 1451 936 863 727 635 511 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4260 4658 4932 5079 5490 4260 Size: 398 274 147 411 4597 Fragments arranged by size: 4597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2191 2552 2582 2699 Size: 30 165 30 758 30 361 30 117 Cuts at: 2699 2729 2741 2771 1178 Size: 30 12 30 4234 Fragments arranged by size: 4234 758 361 165 117 30 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5827 BseYI C'CCAG_C Cuts at: 2462 2863 2870 3039 5030 5526 2462 Size: 401 7 169 1991 496 2763 Fragments arranged by size: 2763 1991 496 401 169 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2310 3277 4038 5066 1540 Size: 275 186 309 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 309 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 3888 3973 5044 2370 Size: 1518 85 1071 3153 Fragments arranged by size: 3153 1518 1071 85 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 BsmBI CGTCTCn'nnnn_ Cuts at: 2112 2744 3008 2112 Size: 632 264 4931 Fragments arranged by size: 4931 632 264 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2179 2301 2370 2856 2870 2939 Size: 399 231 158 122 69 486 14 69 Cuts at: 2939 3046 3146 3888 3973 5044 1391 Size: 107 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 399 231 158 122 107 100 85 69 69 14 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 2967 3182 844 Size: 1268 855 215 3489 Fragments arranged by size: 3489 1268 855 215 BsrBI CCG'CTC Cuts at: 1394 1823 2593 3714 1394 Size: 429 770 1121 3507 Fragments arranged by size: 3507 1121 770 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2472 4471 4658 1700 Size: 110 662 1999 187 2869 Fragments arranged by size: 2869 1999 662 187 110 BsrGI T'GTAC_A Cuts at: 96 2385 96 Size: 2289 3538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 3063 794 Size: 2269 3558 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstXI CCAn_nnnn'nTGG Cuts at: 2947 2947 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2008 2132 2439 3157 3524 3909 3926 5378 Size: 36 124 307 718 367 385 17 1452 Cuts at: 5378 5464 5476 5822 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 718 385 367 346 307 124 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2268 2564 2856 513 Size: 552 939 264 296 292 3484 Fragments arranged by size: 3484 939 552 296 292 264 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2163 2322 3343 4137 4157 2163 Size: 159 1021 794 20 3833 Fragments arranged by size: 3833 1021 794 159 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2055 2307 Size: 54 1425 50 275 18 192 33 252 Cuts at: 2307 2572 2604 2655 3274 4163 5792 8 Size: 265 32 51 619 889 1629 43 Fragments arranged by size: 1629 1425 889 619 275 265 252 192 54 51 50 43 33 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2307 3274 1537 Size: 275 495 967 4090 Fragments arranged by size: 4090 967 495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3757 1353 Size: 2404 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4414 4788 4934 387 Size: 1064 138 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2579 2858 3019 3890 4487 5274 Size: 393 685 262 279 161 871 597 787 Cuts at: 5274 5542 1239 Size: 268 1524 Fragments arranged by size: 1524 871 787 685 597 393 279 268 262 161 EcoNI CCTnn'n_nnAGG Cuts at: 2142 2732 2142 Size: 590 5237 EcoO109I rG'GnC_Cy Cuts at: 2187 2867 2944 2187 Size: 680 77 5070 Fragments arranged by size: 5070 680 77 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 EcoRV GAT'ATC Cuts at: 2380 2380 Size: 5827 FspI TGC'GCA Cuts at: 3104 4333 3104 Size: 1229 4598 HaeII r_GCGC'y Cuts at: 1678 2047 2437 4974 1678 Size: 369 390 2537 2531 Fragments arranged by size: 2537 2531 390 369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 2741 2773 4468 Size: 32 135 32 980 32 157 32 1695 Cuts at: 4468 4500 4542 4574 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 980 157 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 2043 2043 Size: 5827 KpnI G_GTAC'C Cuts at: 2959 3094 3152 2959 Size: 135 58 5634 Fragments arranged by size: 5634 135 58 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 2661 4941 5125 1305 Size: 1251 105 2280 184 2007 Fragments arranged by size: 2280 2007 1251 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4005 Fragments arranged by size: 4005 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2918 2922 3785 4144 4303 518 Size: 648 1101 651 4 863 359 159 2042 Fragments arranged by size: 2042 1101 863 651 648 359 159 4 MspA1I CmG'CkG Cuts at: 1606 2049 2074 2193 2283 3039 3243 3920 5068 Size: 443 25 119 90 756 204 677 1148 Cuts at: 5068 5313 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 756 677 443 245 204 119 90 25 NaeI GCC'GGC Cuts at: 2474 2474 Size: 5827 NarI GG'CG_CC Cuts at: 2044 2044 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2472 2472 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 3185 4730 1132 Size: 97 1956 1545 2229 Fragments arranged by size: 2229 1956 1545 97 PasI CC'CwG_GG Cuts at: 1348 2014 1348 Size: 666 5161 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5827 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PmlI CAC'GTG Cuts at: 2920 2920 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2187 2944 2187 Size: 757 5070 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2017 2866 2017 Size: 849 4978 PstI C_TGCA'G Cuts at: 830 2685 2964 3078 3179 830 Size: 1855 279 114 101 3478 Fragments arranged by size: 3478 1855 279 114 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2074 3039 2074 Size: 965 4862 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SanDI GG'GwC_CC Cuts at: 2187 2187 Size: 5827 SbfI CC_TGCA'GG Cuts at: 2685 2964 3179 2685 Size: 279 215 5333 Fragments arranged by size: 5333 279 215 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SfcI C'TryA_G Cuts at: 826 1045 2681 2960 3074 3175 4310 4991 5182 Size: 219 1636 279 114 101 1135 681 191 Cuts at: 5182 826 Size: 1471 Fragments arranged by size: 1636 1471 1135 681 279 219 191 114 101 SfoI GGC'GCC Cuts at: 2045 2045 Size: 5827 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2693 3931 4832 5094 820 Size: 197 926 750 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 750 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 2843 3062 1517 Size: 1326 219 4282 Fragments arranged by size: 4282 1326 219 StyI C'CwwG_G Cuts at: 513 1065 2004 2138 2733 2940 3266 513 Size: 552 939 134 595 207 326 3074 Fragments arranged by size: 3074 939 595 552 326 207 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2601 2627 3854 3871 4024 4209 4654 Size: 1255 1021 26 1227 17 153 185 445 Cuts at: 4654 325 Size: 1498 Fragments arranged by size: 1498 1255 1227 1021 445 185 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 4073 5785 Size: 274 80 33 51 1443 408 1688 1712 Cuts at: 5785 96 Size: 138 Fragments arranged by size: 1712 1688 1443 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 5787 5819 2161 Size: 32 3594 32 2169 Fragments arranged by size: 3594 2169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2357 2755 1316 Size: 1041 398 4388 Fragments arranged by size: 4388 1041 398 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2037 2614 2011 Size: 26 577 5224 Fragments arranged by size: 5224 577 26 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI