################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BbeI BbrPI BcgI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI EclXI Eco47III Eco52I EcoO109I EcoRI EcoRV FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 1312 CGCG CG^CG AccIII 459 TCCGGA T^CCGGA AcyI 910 GACGCC G[GA]^CG[CT]C AhdI 58 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1771 GTGCAC G^TGCAC ApaLI 1771 GTGCAC G^TGCAC AspEI 58 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 814 TGCGCA TGC^GCA BalI 827 TGGCCA TGG^CCA BanI 1536 GGCACC G^G[CT][GA]CC BbvCI 1351 GCTGAGG CCTCAGC(-5/-2) BclI 930 TGATCA T^GATCA BglII 1358 AGATCT A^GATCT BsaHI 910 GACGCC G[GA]^CG[CT]C BsaWI 459 TCCGGA [AT]^CCGG[AT] BseAI 459 TCCGGA T^CCGGA BspEI 459 TCCGGA T^CCGGA BspHI 665 TCATGA T^CATGA BspMI 1658 ACCTGC ACCTGC(4/8) BsrBI 1171 GAGCGG CCGCTC(-3/-3) BsrI 161 ACTGG ACTGG(1/-1) BssSI 625 CACGAG CACGAG(-5/-1) BstEII 1609 GGTTACC G^GT[ACGT]ACC BstPI 1609 GGTTACC G^GT[ACGT]ACC BstUI 1312 CGCG CG^CG BstXI 23 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1358 AGATCT [GA]^GATC[CT] DraI 178 TTTAAA TTT^AAA DsaI 1430 CCATGG C^C[GA][CT]GG Eam1105I 58 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1609 GGTTACC G^GT[ACGT]ACC EcoT22I 96 ATGCAT ATGCA^T FbaI 930 TGATCA T^GATCA FspI 814 TGCGCA TGC^GCA HaeII 1272 GGCGCT [GA]GCGC^[CT] HgaI 910 GACGC GACGC(5/10) Hin1I 910 GACGCC G[GA]^CG[CT]C MaeIII 1610 GTTAC ^GT[ACGT]AC MflI 1358 AGATCT [GA]^GATC[CT] MluNI 827 TGGCCA TGG^CCA MroI 459 TCCGGA T^CCGGA MscI 827 TGGCCA TGG^CCA MslI 1431 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1312 CGCG CG^CG NcoI 1430 CCATGG C^CATGG NsiI 96 ATGCAT ATGCA^T PaeR7I 973 CTCGAG C^TCGAG RcaI 665 TCATGA T^CATGA StuI 1418 AGGCCT AGG^CCT XhoI 973 CTCGAG C^TCGAG XhoII 1358 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 1554 AAATTC [GA]^AATT[CT] 1573 AAATTC AflIII 197 ACATGT A^C[GA][CT]GT 282 ACATGT AlwI 264 GATCC GGATC(4/5) 543 GGATC AocI 985 CCTGAGG CC^T[ACGT]AGG 2150 CCTCAGG ApoI 1554 AAATTC [GA]^AATT[CT] 1573 AAATTC BanII 820 GAGCTC G[GA]GC[CT]^C 1180 GAGCTC BcnI 1300 CCCGG CC^[GC]GG 1410 CCGGG BglI 1107 GCCCAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1261 GCCCGAGAGGC BsgI 870 GTGCAG GTGCAG(16/14) 1814 GTGCAG BsmI 94 GAATGC GAATGC(1/-1) 564 GAATGC BspLU11I 197 ACATGT A^CATGT 282 ACATGT Bsu36I 985 CCTGAGG CC^T[ACGT]AGG 2150 CCTCAGG Eco81I 985 CCTGAGG CC^T[ACGT]AGG 2150 CCTCAGG EcoT14I 737 CCAAGG C^C[AT][AT]GG 1430 CCATGG MaeII 842 ACGT A^CGT 1845 ACGT NciI 1300 CCCGG CC^[GC]GG 1410 CCGGG PstI 1162 CTGCAG CTGCA^G 1900 CTGCAG SacI 820 GAGCTC GAGCT^C 1180 GAGCTC SfcI 1162 CTGCAG C^T[GA][CT]AG 1900 CTGCAG StyI 737 CCAAGG C^C[AT][AT]GG 1430 CCATGG TspRI 705 CAGTG CA[GC]TG[ACGT][ACGT]^ 880 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoNI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TthHB8I