(Circular) (Six-base) MAPSORT of: pFN21AB6260.seq Check: 111 from: 1 to: 6886 pFN21AB6260 6886 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3672 3672 Size: 6886 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 4185 5013 5386 1563 Size: 2622 828 373 3063 Fragments arranged by size: 3063 2622 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3692 4023 4949 6333 6601 1632 Size: 2060 331 926 1384 268 1917 Fragments arranged by size: 2060 1917 1384 926 331 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 2202 2287 5785 6762 1203 Size: 999 85 3498 977 1327 Fragments arranged by size: 3498 1327 999 977 85 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 2068 5615 2068 Size: 3547 3339 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AlwNI CAG_nnn'CTG Cuts at: 2617 3637 3702 4175 4629 6201 2617 Size: 1020 65 473 454 1572 3302 Fragments arranged by size: 3302 1572 1020 473 454 65 ApaLI G'TGCA_C Cuts at: 3776 4943 6099 3776 Size: 1167 1156 4563 Fragments arranged by size: 4563 1167 1156 ApoI r'AATT_y Cuts at: 1493 3559 3578 4195 4421 4573 6682 1493 Size: 2066 19 617 226 152 2109 1697 Fragments arranged by size: 2109 2066 1697 617 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 2978 3267 3338 4211 1870 Size: 73 1035 289 71 873 4545 Fragments arranged by size: 4545 1035 873 289 73 71 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3541 4207 5662 618 Size: 325 217 627 1754 666 1455 1842 Fragments arranged by size: 1842 1754 1455 666 627 325 217 BanII G_rGCy'C Cuts at: 1391 2829 3189 4205 1391 Size: 1438 360 1016 4072 Fragments arranged by size: 4072 1438 1016 360 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 3507 4092 928 Size: 971 186 1422 585 3722 Fragments arranged by size: 3722 1422 971 585 186 BbvCI CC'TCA_GC Cuts at: 3357 3357 Size: 6886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5077 5111 1903 Size: 34 3140 34 3678 Fragments arranged by size: 3678 3140 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 2935 1548 Size: 1387 5499 BfrBI ATG'CAT Cuts at: 2103 2103 Size: 6886 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3118 3272 5497 Size: 107 122 71 631 605 1446 154 2225 Cuts at: 5497 136 Size: 1525 Fragments arranged by size: 2225 1525 1446 631 605 154 122 107 71 BglII A'GATC_T Cuts at: 3363 6881 3363 Size: 3518 3368 BlpI GC'TnA_GC Cuts at: 4303 4303 Size: 6886 Bme1580I G_kGCm'C Cuts at: 1790 2853 3544 3780 4947 6103 1790 Size: 1063 691 236 1167 1156 2573 Fragments arranged by size: 2573 1167 1156 1063 691 236 BmgBI CAC'GTC Cuts at: 1318 2848 1318 Size: 1530 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 4617 5575 Size: 416 53 83 399 24 759 2441 958 Cuts at: 5575 442 Size: 1753 Fragments arranged by size: 2441 1753 958 759 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3822 3856 3822 Size: 34 6852 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 3531 4126 5546 1239 Size: 978 1314 595 1420 2579 Fragments arranged by size: 2579 1420 1314 978 595 Bpu10I CC'TnA_GC Cuts at: 2280 3183 3357 2280 Size: 903 174 5809 Fragments arranged by size: 5809 903 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2303 2744 3266 5011 5876 6174 2303 Size: 441 522 1745 865 298 3015 Fragments arranged by size: 3015 1745 865 522 441 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5549 882 Size: 617 4050 2219 Fragments arranged by size: 4050 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4677 493 Size: 711 3473 2702 Fragments arranged by size: 3473 2702 711 BsaBI GATnn'nnATC Cuts at: 6880 6880 Size: 6886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2916 4693 5075 5710 Size: 53 83 186 936 1383 1777 382 635 Cuts at: 5710 275 Size: 1451 Fragments arranged by size: 1777 1451 1383 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2464 5319 5717 5991 6138 6549 2464 Size: 2855 398 274 147 411 2801 Fragments arranged by size: 2855 2801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3626 3656 4163 4193 1178 Size: 30 165 30 2223 30 507 30 3871 Fragments arranged by size: 3871 2223 507 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2729 3008 3011 3164 3188 3374 3767 3941 Size: 1543 279 3 153 24 186 393 174 Cuts at: 3941 3988 4009 4138 4141 4144 4156 1186 Size: 47 21 129 3 3 12 3916 Fragments arranged by size: 3916 1543 393 279 186 174 153 129 47 24 21 12 3 3 3 BseYI C'CCAG_C Cuts at: 2610 2852 6089 6585 2610 Size: 242 3237 496 2911 Fragments arranged by size: 3237 2911 496 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2896 3840 1163 Size: 573 1160 944 4209 Fragments arranged by size: 4209 1160 944 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2829 3189 3735 3780 4947 5032 6103 2829 Size: 360 546 45 1167 85 1071 3612 Fragments arranged by size: 3612 1167 1071 546 360 85 45 BsmI GAATG_Cn' Cuts at: 2105 2575 4397 4490 2105 Size: 470 1822 93 4501 Fragments arranged by size: 4501 1822 470 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2829 2853 3189 3544 3735 3780 4205 Size: 399 1039 24 336 355 191 45 425 Cuts at: 4205 4947 5032 6103 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1039 742 425 399 355 336 191 85 45 24 BspEI T'CCGG_A Cuts at: 2464 6549 2464 Size: 4085 2801 BspHI T'CATG_A Cuts at: 2670 4775 6505 2670 Size: 2105 1730 3051 Fragments arranged by size: 3051 2105 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3672 4241 844 Size: 2828 569 3489 Fragments arranged by size: 3489 2828 569 BsrBI CCG'CTC Cuts at: 1394 1823 3178 4773 1394 Size: 429 1355 1595 3507 Fragments arranged by size: 3507 1595 1355 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5381 5555 65 Size: 1183 4133 174 1396 Fragments arranged by size: 4133 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 2630 4946 5958 2630 Size: 2316 1012 3558 Fragments arranged by size: 3558 2316 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6886 BstBI TT'CG_AA Cuts at: 5690 5690 Size: 6886 BstEII G'GTnAC_C Cuts at: 1184 3614 1184 Size: 2430 4456 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 6886 BstYI r'GATC_y Cuts at: 1972 3363 4216 4583 4968 4985 6437 6523 6535 Size: 1391 853 367 385 17 1452 86 12 Cuts at: 6535 6881 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1391 853 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2991 4156 2991 Size: 1165 5721 BtgI C'CryG_G Cuts at: 513 1065 3435 513 Size: 552 2370 3964 Fragments arranged by size: 3964 2370 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 2891 4402 5196 5216 2891 Size: 1511 794 20 4561 Fragments arranged by size: 4561 1511 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 2185 4190 2185 Size: 2005 4881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2657 2832 Size: 54 1425 50 275 18 213 614 175 Cuts at: 2832 3129 3747 4333 5222 6851 8 Size: 297 618 586 889 1629 43 Fragments arranged by size: 1629 1425 889 618 614 586 297 275 213 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3015 3933 3966 4062 4816 1353 Size: 1662 918 33 96 754 3423 Fragments arranged by size: 3423 1662 918 754 96 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5473 5847 5993 387 Size: 1064 138 3884 374 146 1280 Fragments arranged by size: 3884 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2827 3187 2827 Size: 360 6526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 3531 3692 4023 4126 4949 5546 Size: 393 585 1314 161 331 103 823 597 Cuts at: 5546 6333 6601 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1314 823 787 597 585 393 331 268 161 103 EcoNI CCTnn'n_nnAGG Cuts at: 2304 2741 2990 2304 Size: 437 249 6200 Fragments arranged by size: 6200 437 249 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FspI TGC'GCA Cuts at: 2821 5392 2821 Size: 2571 4315 HaeII r_GCGC'y Cuts at: 1678 3281 6033 1678 Size: 1603 2752 2531 Fragments arranged by size: 2752 2531 1603 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2667 2699 3626 Size: 32 135 32 482 32 581 32 927 Cuts at: 3626 3658 3682 3714 4109 4141 4163 4195 5527 Size: 32 24 32 395 32 22 32 1332 Cuts at: 5527 5559 5601 5633 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1332 927 581 482 395 135 42 32 32 32 32 32 32 32 32 32 32 24 22 HincII GTy'rAC Cuts at: 669 1657 1938 4230 4476 669 Size: 988 281 2292 246 3079 Fragments arranged by size: 3079 2292 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6886 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 4485 4485 Size: 6886 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3273 3897 4206 6000 6184 1305 Size: 1968 624 309 1794 184 2007 Fragments arranged by size: 2007 1968 1794 624 309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2834 10 Size: 54 1425 343 1002 4062 Fragments arranged by size: 4062 1425 1002 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3440 4844 5203 5362 518 Size: 648 2274 1404 359 159 2042 Fragments arranged by size: 2274 2042 1404 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2617 3007 3166 3551 3670 4302 4979 6127 Size: 1011 390 159 385 119 632 677 1148 Cuts at: 6127 6372 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1011 677 632 390 385 245 159 119 NcoI C'CATG_G Cuts at: 513 1065 3435 513 Size: 552 2370 3964 Fragments arranged by size: 3964 2370 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NsiI A_TGCA'T Cuts at: 2105 2105 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 2086 2206 2291 4244 5789 1132 Size: 97 857 120 85 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 857 120 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 2202 2287 5785 2202 Size: 85 3498 3303 Fragments arranged by size: 3498 3303 85 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2742 2774 2792 2824 4955 4987 6468 6500 2742 Size: 32 18 32 2131 32 1481 32 3128 Fragments arranged by size: 3128 2131 1481 32 32 32 32 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PstI C_TGCA'G Cuts at: 830 3171 3909 4238 830 Size: 2341 738 329 3478 Fragments arranged by size: 3478 2341 738 329 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 2617 3166 3551 2617 Size: 549 385 5952 Fragments arranged by size: 5952 549 385 SacI G_AGCT'C Cuts at: 2829 3189 2829 Size: 360 6526 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 3167 3905 4234 5369 6050 6241 826 Size: 219 2122 738 329 1135 681 191 1471 Fragments arranged by size: 2122 1471 1135 738 681 329 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2318 2759 2978 3281 4990 5891 Size: 197 926 375 441 219 303 1709 901 Cuts at: 5891 6153 820 Size: 262 1553 Fragments arranged by size: 1709 1553 926 901 441 375 303 262 219 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 3425 1517 Size: 1908 4978 StyI C'CwwG_G Cuts at: 513 1065 2742 3435 4325 513 Size: 552 1677 693 890 3074 Fragments arranged by size: 3074 1677 890 693 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2209 2343 2642 2709 4913 4930 5083 Size: 1255 629 134 299 67 2204 17 153 Cuts at: 5083 5268 5713 325 Size: 185 445 1498 Fragments arranged by size: 2204 1498 1255 629 445 299 185 153 134 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 3258 3768 Size: 274 80 33 51 1443 837 444 510 Cuts at: 3768 3834 5132 6844 96 Size: 66 1298 1712 138 Fragments arranged by size: 1712 1443 1298 837 510 444 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6846 6878 6846 Size: 32 6854 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XhoI C'TCGA_G Cuts at: 1943 2978 1943 Size: 1035 5851 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 5015 5015 Size: 6886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI