Restriction map for
pFN21AB6260
(Linear) (Six-base) MAPSORT of: KIBB6260.seq Check: 9066 from: 1 to: 2181
KIBB6260.orf
With 182 enzymes: SgfI *
July 3, 2012 16:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1667 2181
Size: 1667 514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1687 2018 2181
Size: 1687 331 163
Fragments arranged by size:
1687 331 163
AflIII A'CryG_T
Cuts at: 0 197 282 2181
Size: 197 85 1899
Fragments arranged by size:
1899 197 85
AhdI GACnn_n'nnGTC
Cuts at: 0 63 2181
Size: 63 2118
AlwNI CAG_nnn'CTG
Cuts at: 0 612 1632 1697 2170 2181
Size: 612 1020 65 473 11
Fragments arranged by size:
1020 612 473 65 11
ApaLI G'TGCA_C
Cuts at: 0 1771 2181
Size: 1771 410
ApoI r'AATT_y
Cuts at: 0 1554 1573 2181
Size: 1554 19 608
Fragments arranged by size:
1554 608 19
AvaI C'yCGr_G
Cuts at: 0 973 1262 1333 2181
Size: 973 289 71 848
Fragments arranged by size:
973 848 289 71
BanI G'GyrC_C
Cuts at: 0 1536 2181
Size: 1536 645
BanII G_rGCy'C
Cuts at: 0 824 1184 2181
Size: 824 360 997
Fragments arranged by size:
997 824 360
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 1502 2087 2181
Size: 80 1422 585 94
Fragments arranged by size:
1422 585 94 80
BbvCI CC'TCA_GC
Cuts at: 0 1352 2181
Size: 1352 829
BclI T'GATC_A
Cuts at: 0 930 2181
Size: 930 1251
BfrBI ATG'CAT
Cuts at: 0 98 2181
Size: 98 2083
BglI GCCn_nnn'nGGC
Cuts at: 0 1113 1267 2181
Size: 1113 154 914
Fragments arranged by size:
1113 914 154
BglII A'GATC_T
Cuts at: 0 1358 2181
Size: 1358 823
Bme1580I G_kGCm'C
Cuts at: 0 848 1539 1775 2181
Size: 848 691 236 406
Fragments arranged by size:
848 691 406 236
BmgBI CAC'GTC
Cuts at: 0 843 2181
Size: 843 1338
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 2181
Size: 171 2010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1817 1851 2181
Size: 1817 34 330
Fragments arranged by size:
1817 330 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1526 2121 2181
Size: 212 1314 595 60
Fragments arranged by size:
1314 595 212 60
Bpu10I CC'TnA_GC
Cuts at: 0 275 1178 1352 2181
Size: 275 903 174 829
Fragments arranged by size:
903 829 275 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 739 1261 2181
Size: 298 441 522 920
Fragments arranged by size:
920 522 441 298
BsaHI Gr'CG_yC
Cuts at: 0 911 2181
Size: 911 1270
BsaWI w'CCGG_w
Cuts at: 0 459 2181
Size: 459 1722
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1621 1651 2158 2181
Size: 1621 30 507 23
Fragments arranged by size:
1621 507 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 724 1003 1006 1159 1183 1369 1762 1936
Size: 724 279 3 153 24 186 393 174
Cuts at: 1936 1983 2004 2133 2136 2139 2151 2181
Size: 47 21 129 3 3 12 30
Fragments arranged by size:
724 393 279 186 174 153 129 47
30 24 21 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 605 847 2181
Size: 605 242 1334
Fragments arranged by size:
1334 605 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1835 2181
Size: 891 944 346
Fragments arranged by size:
944 891 346
BsiHKAI G_wGCw'C
Cuts at: 0 824 1184 1730 1775 2181
Size: 824 360 546 45 406
Fragments arranged by size:
824 546 406 360 45
BsmI GAATG_Cn'
Cuts at: 0 100 570 2181
Size: 100 470 1611
Fragments arranged by size:
1611 470 100
Bsp1286I G_dGCh'C
Cuts at: 0 824 848 1184 1539 1730 1775 2181
Size: 824 24 336 355 191 45 406
Fragments arranged by size:
824 406 355 336 191 45 24
BspEI T'CCGG_A
Cuts at: 0 459 2181
Size: 459 1722
BspHI T'CATG_A
Cuts at: 0 665 2181
Size: 665 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1667 2181
Size: 1667 514
BsrBI CCG'CTC
Cuts at: 0 1173 2181
Size: 1173 1008
BssSI C'ACGA_G
Cuts at: 0 625 2181
Size: 625 1556
BstEII G'GTnAC_C
Cuts at: 0 1609 2181
Size: 1609 572
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 2181
Size: 30 2151
BstYI r'GATC_y
Cuts at: 0 1358 2181
Size: 1358 823
Bsu36I CC'TnA_GG
Cuts at: 0 986 2151 2181
Size: 986 1165 30
Fragments arranged by size:
1165 986 30
BtgI C'CryG_G
Cuts at: 0 1430 2181
Size: 1430 751
BtsI GCAGTG_nn'
Cuts at: 0 886 2181
Size: 886 1295
DraI TTT'AAA
Cuts at: 0 180 2181
Size: 180 2001
EaeI y'GGCC_r
Cuts at: 0 38 652 827 1124 1742 2181
Size: 38 614 175 297 618 439
Fragments arranged by size:
618 614 439 297 175 38
EarI CTCTTCn'nnn_
Cuts at: 0 1010 1928 1961 2057 2181
Size: 1010 918 33 96 124
Fragments arranged by size:
1010 918 124 96 33
EcoICRI GAG'CTC
Cuts at: 0 822 1182 2181
Size: 822 360 999
Fragments arranged by size:
999 822 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1526 1687 2018 2121 2181
Size: 212 1314 161 331 103 60
Fragments arranged by size:
1314 331 212 161 103 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 299 736 985 2181
Size: 299 437 249 1196
Fragments arranged by size:
1196 437 299 249
FspI TGC'GCA
Cuts at: 0 816 2181
Size: 816 1365
HaeII r_GCGC'y
Cuts at: 0 1276 2181
Size: 1276 905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 662 694 1621 1653 1677 1709
Size: 49 32 581 32 927 32 24 32
Cuts at: 1709 2104 2136 2158 2181
Size: 395 32 22 23
Fragments arranged by size:
927 581 395 49 32 32 32 32
32 24 23 22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1892 2181
Size: 1268 624 289
Fragments arranged by size:
1268 624 289
MscI TGG'CCA
Cuts at: 0 829 2181
Size: 829 1352
MslI CAynn'nnrTG
Cuts at: 0 1435 2181
Size: 1435 746
MspA1I CmG'CkG
Cuts at: 0 612 1002 1161 1546 1665 2181
Size: 612 390 159 385 119 516
Fragments arranged by size:
612 516 390 385 159 119
NcoI C'CATG_G
Cuts at: 0 1430 2181
Size: 1430 751
NsiI A_TGCA'T
Cuts at: 0 100 2181
Size: 100 2081
NspI r_CATG'y
Cuts at: 0 81 201 286 2181
Size: 81 120 85 1895
Fragments arranged by size:
1895 120 85 81
PciI A'CATG_T
Cuts at: 0 197 282 2181
Size: 197 85 1899
Fragments arranged by size:
1899 197 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 737 769 787 819 2181
Size: 737 32 18 32 1362
Fragments arranged by size:
1362 737 32 32 18
PstI C_TGCA'G
Cuts at: 0 1166 1904 2181
Size: 1166 738 277
Fragments arranged by size:
1166 738 277
PvuII CAG'CTG
Cuts at: 0 612 1161 1546 2181
Size: 612 549 385 635
Fragments arranged by size:
635 612 549 385
SacI G_AGCT'C
Cuts at: 0 824 1184 2181
Size: 824 360 997
Fragments arranged by size:
997 824 360
SfcI C'TryA_G
Cuts at: 0 1162 1900 2181
Size: 1162 738 281
Fragments arranged by size:
1162 738 281
SmlI C'TyrA_G
Cuts at: 0 313 754 973 1276 2181
Size: 313 441 219 303 905
Fragments arranged by size:
905 441 313 303 219
StuI AGG'CCT
Cuts at: 0 1420 2181
Size: 1420 761
StyI C'CwwG_G
Cuts at: 0 737 1430 2181
Size: 737 693 751
Fragments arranged by size:
751 737 693
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 338 637 704 2181
Size: 204 134 299 67 1477
Fragments arranged by size:
1477 299 204 134 67
TatI w'GTAC_w
Cuts at: 0 809 1253 1763 1829 2181
Size: 809 444 510 66 352
Fragments arranged by size:
809 510 444 352 66
XhoI C'TCGA_G
Cuts at: 0 973 2181
Size: 973 1208
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BclI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI FspI HaeII Hin4I MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PpiI PstI PvuII SacI
SfcI SmlI StuI StyI TaqII TatI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BlpI BmtI BsaI BsaAI
BsaBI BsiEI BsiWI BsmBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI