################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccIII AflIII AgeI AlwNI AscI AseI AsnI Asp700I Asp718I AviII BalI BanI BbeI BcgI BciVI BlpI Bpu1102I BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BssHII BstAPI BstBI BstEII BstPI BstXI CelII ClaI CpoI DraI DraIII DrdI EarI EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI Ksp632I MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 73 enzymes) # ################################### AccI 297 GTATAC GT^[AC][GT]AC AclI 1294 AACGTT AA^CGTT AcsI 363 AAATTC [GA]^AATT[CT] AcyI 13 GACGCC G[GA]^CG[CT]C AflII 1132 CTTAAG C^TTAAG AhdI 1624 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 736 GTGCAC G^TGCAC AocI 36 CCTAAGG CC^T[ACGT]AGG Aor51HI 324 AGCGCT AGC^GCT ApaI 1070 GGGCCC GGGCC^C ApaLI 736 GTGCAC G^TGCAC ApoI 363 AAATTC [GA]^AATT[CT] AspEI 1624 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 736 GTGCAC G[AT]GC[AT]^C AspI 1522 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1067 CCCGGG C^[CT]CG[GA]G AvrII 1542 CCTAGG C^CTAGG BanII 1070 GGGCCC G[GA]GC[CT]^C BbrPI 118 CACGTG CAC^GTG BclI 1005 TGATCA T^GATCA BfrI 1132 CTTAAG C^TTAAG BglI 1759 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 834 AGATCT A^GATCT BlnI 1542 CCTAGG C^CTAGG BsaAI 118 CACGTG [CT]AC^GT[GA] BsaBI 901 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 13 GACGCC G[GA]^CG[CT]C BsaI 348 GAGACC GGTCTC(1/5) BsgI 145 CTGCAC GTGCAG(16/14) BsiEI 134 CGGCCG CG[GA][CT]^CG BsiHKAI 736 GTGCAC G[AT]GC[AT]^C BsmI 282 GAATGC GAATGC(1/-1) BsoBI 1067 CCCGGG C^[CT]CG[GA]G Bsp1407I 533 TGTACA T^GTACA BspHI 264 TCATGA T^CATGA BsrFI 50 ACCGGC [GA]^CCGG[CT] BsrGI 533 TGTACA T^GTACA BssSI 947 CACGAG CACGAG(-5/-1) Bst1107I 297 GTATAC GTA^TAC BstZ17I 297 GTATAC GTA^TAC Bsu36I 36 CCTAAGG CC^T[ACGT]AGG Cfr10I 50 ACCGGC [GA]^CCGG[CT] EaeI 134 CGGCCG [CT]^GGCC[GA] EagI 134 CGGCCG C^GGCCG Eam1105I 1624 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 134 CGGCCG C^GGCCG Eco47III 324 AGCGCT AGC^GCT Eco52I 134 CGGCCG C^GGCCG Eco81I 36 CCTAAGG CC^T[ACGT]AGG FbaI 1005 TGATCA T^GATCA HgaI 13 GACGC GACGC(5/10) Hin1I 13 GACGCC G[GA]^CG[CT]C HindIII 825 AAGCTT A^AGCTT MamI 901 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 302 CCATGG C^CATGG NotI 133 GCGGCCGC GC^GGCCGC PflFI 1522 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 231 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 118 CACGTG CAC^GTG PmlI 118 CACGTG CAC^GTG PpuMI 1730 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1294 AACGTT AA^CGTT PvuII 1099 CAGCTG CAG^CTG RcaI 264 TCATGA T^CATGA SfcI 1050 CTATAG C^T[GA][CT]AG SmaI 1067 CCCGGG CCC^GGG SspBI 533 TGTACA T^GTACA TfiI 253 GATTC G^A[AT]TC Tth111I 1522 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 231 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1635 TCTAGA T^CTAGA XmaCI 1067 CCCGGG C^CCGGG XmaI 1067 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 84 CGCG CG^CG 138 CGCG BpmI 851 CTGGAG CTGGAG(16/14) 1735 CTGGAG BstNI 1734 CCTGG CC^[AT]GG 1773 CCAGG BstUI 84 CGCG CG^CG 138 CGCG CfoI 325 GCGC GCG^C 555 GCGC DraII 1070 GGGCCCT [GA]G^G[ACGT]CC[CT] 1730 GGGACCT EcoO109I 1070 GGGCCCT [GA]G^G[ACGT]CC[CT] 1730 GGGACCT EcoRII 1734 CCTGG ^CC[AT]GG 1773 CCAGG HaeII 324 AGCGCT [GA]GCGC^[CT] 554 AGCGCC HhaI 325 GCGC GCG^C 555 GCGC HinP1I 325 GCGC G^CGC 555 GCGC KspI 83 CCGCGG CCGC^GG 137 CCGCGG MseI 1133 TTAA T^TAA 1427 TTAA MslI 663 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 739 CACACTGGTG MvaI 1734 CCTGG CC^[AT]GG 1773 CCAGG MvnI 84 CGCG CG^CG 138 CGCG NspI 1011 GCATGC [GA]CATG^[CT] 1790 GCATGC SacII 83 CCGCGG CCGC^GG 137 CCGCGG SphI 1011 GCATGC GCATG^C 1790 GCATGC Tru9I 1133 TTAA T^TAA 1427 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BamHI BbsI BbvCI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII