(Circular) (Six-base) MAPSORT of: pFN21AE2021.seq Check: 9466 from: 1 to: 6502 pFN21AE2021 6502 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2303 3845 1937 Size: 366 1542 4594 Fragments arranged by size: 4594 1542 366 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 3300 4629 5002 1563 Size: 1737 1329 373 3063 Fragments arranged by size: 3063 1737 1329 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 2833 4565 5949 6217 1632 Size: 918 283 1732 1384 268 1917 Fragments arranged by size: 1917 1732 1384 918 283 268 AfeI AGC'GCT Cuts at: 2331 2331 Size: 6502 AflII C'TTAA_G Cuts at: 820 1017 3137 820 Size: 197 2120 4185 Fragments arranged by size: 4185 2120 197 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 3634 5231 3634 Size: 1597 4905 AleI CACnn'nnGTG Cuts at: 1166 2748 1166 Size: 1582 4920 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6502 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3773 3805 3773 Size: 32 6470 AlwNI CAG_nnn'CTG Cuts at: 4245 5817 4245 Size: 1572 4930 ApaI G_GGCC'C Cuts at: 3079 3079 Size: 6502 ApaLI G'TGCA_C Cuts at: 2741 4559 5715 2741 Size: 1818 1156 3528 Fragments arranged by size: 3528 1818 1156 ApoI r'AATT_y Cuts at: 1493 2368 3811 4037 4189 6298 1493 Size: 875 1443 226 152 2109 1697 Fragments arranged by size: 2109 1697 1443 875 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 3072 3827 1870 Size: 73 1129 755 4545 Fragments arranged by size: 4545 1129 755 73 AvrII C'CTAG_G Cuts at: 3547 3547 Size: 6502 BamHI G'GATC_C Cuts at: 2046 2487 2905 3543 3832 4199 2046 Size: 441 418 638 289 367 4349 Fragments arranged by size: 4349 638 441 418 367 289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3823 5278 618 Size: 325 217 627 2036 1455 1842 Fragments arranged by size: 2036 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3079 3821 1391 Size: 1688 742 4072 Fragments arranged by size: 4072 1688 742 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2390 3369 928 Size: 971 129 362 979 4061 Fragments arranged by size: 4061 979 971 362 129 BbvCI CC'TCA_GC Cuts at: 2171 2591 3500 2171 Size: 420 909 5173 Fragments arranged by size: 5173 909 420 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4693 4727 1903 Size: 34 2756 34 3678 Fragments arranged by size: 3678 2756 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 3010 1548 Size: 1462 5040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3770 5113 136 Size: 107 122 71 631 605 2098 1343 1525 Fragments arranged by size: 2098 1525 1343 631 605 122 107 71 BglII A'GATC_T Cuts at: 2839 6497 2839 Size: 3658 2844 BlpI GC'TnA_GC Cuts at: 3919 3919 Size: 6502 Bme1580I G_kGCm'C Cuts at: 1790 2603 2745 3079 4563 5719 1790 Size: 813 142 334 1484 1156 2573 Fragments arranged by size: 2573 1484 1156 813 334 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3362 3760 4233 Size: 416 53 83 399 24 1945 398 473 Cuts at: 4233 5191 442 Size: 958 1753 Fragments arranged by size: 1945 1753 958 473 416 399 398 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 6502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2877 3761 5162 1239 Size: 1638 884 1401 2579 Fragments arranged by size: 2579 1638 1401 884 Bpu10I CC'TnA_GC Cuts at: 2171 2591 3500 2171 Size: 420 909 5173 Fragments arranged by size: 5173 909 420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2743 2864 4627 5492 5790 2743 Size: 121 1763 865 298 3455 Fragments arranged by size: 3455 1763 865 298 121 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2347 5165 882 Size: 617 848 2818 2219 Fragments arranged by size: 2818 2219 848 617 BsaAI yAC'GTr Cuts at: 493 1204 2125 4293 493 Size: 711 921 2168 2702 Fragments arranged by size: 2702 2168 921 711 BsaBI GATnn'nnATC Cuts at: 2910 6496 2910 Size: 3586 2916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 4309 4691 5326 Size: 53 83 186 936 486 2290 382 635 Cuts at: 5326 275 Size: 1451 Fragments arranged by size: 2290 1451 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4935 5333 5607 5754 6165 4935 Size: 398 274 147 411 5272 Fragments arranged by size: 5272 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3245 3275 3773 3803 1178 Size: 30 165 30 1842 30 498 30 3877 Fragments arranged by size: 3877 1842 498 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 3683 3710 1186 Size: 973 1524 27 3978 Fragments arranged by size: 3978 1524 973 27 BseYI C'CCAG_C Cuts at: 5705 6201 5705 Size: 496 6006 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 5530 Fragments arranged by size: 5530 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 3952 4713 5741 1540 Size: 275 186 141 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 141 BsiHKAI G_wGCw'C Cuts at: 2745 4563 4648 5719 2745 Size: 1818 85 1071 3528 Fragments arranged by size: 3528 1818 1071 85 BsmI GAATG_Cn' Cuts at: 2293 4013 4106 2293 Size: 1720 93 4689 Fragments arranged by size: 4689 1720 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2603 2745 3079 3821 4563 4648 5719 Size: 399 813 142 334 742 742 85 1071 Cuts at: 5719 1391 Size: 2174 Fragments arranged by size: 2174 1071 813 742 742 399 334 142 85 BspEI T'CCGG_A Cuts at: 6165 6165 Size: 6502 BspHI T'CATG_A Cuts at: 2269 4391 6121 2269 Size: 2122 1730 2650 Fragments arranged by size: 2650 2122 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3790 3857 844 Size: 2946 67 3489 Fragments arranged by size: 3489 2946 67 BsrBI CCG'CTC Cuts at: 1394 1823 4389 1394 Size: 429 2566 3507 Fragments arranged by size: 3507 2566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2469 3288 3594 4997 5171 65 Size: 1183 1221 819 306 1403 174 1396 Fragments arranged by size: 1403 1396 1221 1183 819 306 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2055 5146 5333 1700 Size: 110 245 3091 187 2869 Fragments arranged by size: 3091 2869 245 187 110 BsrGI T'GTAC_A Cuts at: 96 2538 96 Size: 2442 4060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 2952 4562 5574 2952 Size: 1610 1012 3880 Fragments arranged by size: 3880 1610 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6502 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2046 2487 2839 2905 3543 3832 4199 4584 Size: 74 441 352 66 638 289 367 385 Cuts at: 4584 4601 6053 6139 6151 6497 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 638 441 385 367 352 346 289 86 74 66 17 12 BstZ17I GTA'TAC Cuts at: 2304 2304 Size: 6502 Bsu36I CC'TnA_GG Cuts at: 2042 2042 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2142 2307 3026 3678 513 Size: 552 939 84 54 165 719 652 3337 Fragments arranged by size: 3337 939 719 652 552 165 84 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5763 Fragments arranged by size: 5763 683 56 BtsI GCAGTG_nn' Cuts at: 2603 3791 4018 4812 4832 2603 Size: 1188 227 794 20 4273 Fragments arranged by size: 4273 1188 794 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 3949 4838 Size: 54 1425 50 275 18 309 1810 889 Cuts at: 4838 6467 8 Size: 1629 43 Fragments arranged by size: 1810 1629 1425 889 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2139 3949 1537 Size: 275 327 1810 4090 Fragments arranged by size: 4090 1810 327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4432 1353 Size: 3079 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2550 2833 2877 3761 4565 5162 5949 Size: 393 918 283 44 884 804 597 787 Cuts at: 5949 6217 1239 Size: 268 1524 Fragments arranged by size: 1524 918 884 804 787 597 393 283 268 44 EcoO109I rG'GnC_Cy Cuts at: 3076 3736 3076 Size: 660 5842 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2358 2358 Size: 6502 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2333 2563 5649 1678 Size: 655 230 3086 2531 Fragments arranged by size: 3086 2531 655 230 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 2696 2728 2780 Size: 32 135 32 1076 32 16 32 52 Cuts at: 2780 2812 2890 2922 3620 3652 5143 5175 5217 Size: 32 78 32 698 32 1491 32 42 Cuts at: 5217 5249 1373 Size: 32 2626 Fragments arranged by size: 2626 1491 1076 698 135 78 52 42 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3846 4092 669 Size: 988 281 1908 246 3079 Fragments arranged by size: 3079 1908 988 281 246 HindIII A'AGCT_T Cuts at: 748 2830 748 Size: 2082 4420 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 2883 3288 5616 5800 1305 Size: 876 702 405 2328 184 2007 Fragments arranged by size: 2328 2007 876 702 405 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4680 Fragments arranged by size: 4680 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2672 2748 4460 4819 4978 518 Size: 648 1506 76 1712 359 159 2042 Fragments arranged by size: 2042 1712 1506 648 359 159 76 MspA1I CmG'CkG Cuts at: 1606 2083 2090 2144 3106 3918 4595 5743 5988 Size: 477 7 54 962 812 677 1148 245 Cuts at: 5988 1606 Size: 2120 Fragments arranged by size: 2120 1148 962 812 677 477 245 54 7 NcoI C'CATG_G Cuts at: 513 1065 2004 2307 513 Size: 552 939 303 4708 Fragments arranged by size: 4708 939 552 303 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 2139 3949 2139 Size: 1810 4692 NspI r_CATG'y Cuts at: 1132 1229 3020 3799 3860 5405 1132 Size: 97 1791 779 61 1545 2229 Fragments arranged by size: 2229 1791 1545 779 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6502 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 1108 Size: 1134 5368 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PmlI CAC'GTG Cuts at: 2125 2125 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 4571 4603 6084 6116 3773 Size: 32 766 32 1481 32 4159 Fragments arranged by size: 4159 1481 766 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3736 3736 Size: 6502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PspOMI G'GGCC_C Cuts at: 3075 3075 Size: 6502 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2764 2796 2764 Size: 32 6470 PstI C_TGCA'G Cuts at: 830 3854 830 Size: 3024 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 3106 3106 Size: 6502 SacII CC_GC'GG Cuts at: 2091 2145 2091 Size: 54 6448 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SfcI C'TryA_G Cuts at: 826 1045 3055 3850 4985 5666 5857 826 Size: 219 2010 795 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 795 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3074 3829 1872 Size: 1202 755 4545 Fragments arranged by size: 4545 1202 755 SmlI C'TyrA_G Cuts at: 820 1017 1943 2758 2879 3137 4606 5507 5769 Size: 197 926 815 121 258 1469 901 262 Cuts at: 5769 820 Size: 1553 Fragments arranged by size: 1553 1469 926 901 815 262 258 197 121 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3020 3799 3860 1132 Size: 1888 779 61 3774 Fragments arranged by size: 3774 1888 779 61 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6502 StyI C'CwwG_G Cuts at: 513 1065 2004 2307 2983 3093 3547 3941 513 Size: 552 939 303 676 110 454 394 3074 Fragments arranged by size: 3074 939 676 552 454 394 303 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3019 4529 4546 4699 4884 5329 325 Size: 1255 1439 1510 17 153 185 445 1498 Fragments arranged by size: 1510 1498 1439 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 2844 4748 Size: 274 80 33 51 1443 561 306 1904 Cuts at: 4748 6460 96 Size: 1712 138 Fragments arranged by size: 1904 1712 1443 561 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 3530 1316 Size: 2214 4288 XbaI T'CTAG_A Cuts at: 3640 3838 3640 Size: 198 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 3072 3827 1870 Size: 1202 755 4545 Fragments arranged by size: 4545 1202 755 XmnI GAAnn'nnTTC Cuts at: 4631 4631 Size: 6502 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI