Restriction map for
pFN21AB9428
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HindIII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 607 GACGTC GACGT^C
AccI 610 GTCTAC GT^[AC][GT]AC
AcyI 607 GACGTC G[GA]^CG[CT]C
AfaI 687 GTAC GT^AC
AspI 519 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 623 GGATCC G^GATCC
BbsI 856 GTCTTC GAAGAC(2/6)
BlpI 727 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 727 GCTGAGC GC^T[ACGT]AGC
BpuAI 856 GTCTTC GAAGAC(2/6)
BsaHI 607 GACGTC G[GA]^CG[CT]C
BsaI 1198 GAGACC GGTCTC(1/5)
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BsiEI 1339 CGACCG CG[GA][CT]^CG
BssSI 682 CTCGTG CACGAG(-5/-1)
BstAPI 637 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 727 GCTGAGC GC^T[ACGT]AGC
EaeI 340 TGGCCG [CT]^GGCC[GA]
EarI 650 GAAGAG CTCTTC(1/4)
EcoNI 1514 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 208 GAATTC G^AATTC
EcoRV 168 GATATC GAT^ATC
HgaI 8 GCGTC GACGC(5/10)
Hin1I 607 GACGTC G[GA]^CG[CT]C
Ksp632I 650 GAAGAG CTCTTC(1/4)
MspA1I 24 CAGCGG C[AC]G^C[GT]G
NaeI 267 GCCGGC GCC^GGC
NcoI 188 CCATGG C^CATGG
NgoMIV 267 GCCGGC G^CCGGC
PflFI 519 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 687 GTAC GT^AC
SacI 73 GAGCTC GAGCT^C
SbfI 1304 CCTGCAGG CCTGCA^GG
SfaNI 1221 GATGC GCATC(5/9)
SmlI 427 CTCAAG C^T[CT][GA]AG
Sse8387I 1304 CCTGCAGG CCTGCA^GG
StuI 590 AGGCCT AGG^CCT
Tth111I 519 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1059 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 208 GAATTC [GA]^AATT[CT]
283 AAATTC
ApoI 208 GAATTC [GA]^AATT[CT]
283 AAATTC
AspHI 73 GAGCTC G[AT]GC[AT]^C
409 GTGCTC
AvaI 1095 CCCGAG C^[CT]CG[GA]G
1262 CCCGAG
BanII 73 GAGCTC G[GA]GC[CT]^C
805 GAGCCC
BfaI 223 CTAG C^TAG
1060 CTAG
BsiHKAI 73 GAGCTC G[AT]GC[AT]^C
409 GTGCTC
BsmI 832 GAATGC GAATGC(1/-1)
882 GAATGC
BsoBI 1095 CCCGAG C^[CT]CG[GA]G
1262 CCCGAG
BspMI 389 ACCTGC ACCTGC(4/8)
1131 GCAGGT
BsrBI 343 CCGCTC CCGCTC(-3/-3)
1342 CCGCTC
BsrFI 267 GCCGGC [GA]^CCGG[CT]
713 ACCGGC
BstXI 1138 CCACAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1525 CCACAGGGTTGG
CfoI 112 GCGC GCG^C
219 GCGC
Cfr10I 267 GCCGGC [GA]^CCGG[CT]
713 ACCGGC
DsaI 188 CCATGG C^C[GA][CT]GG
896 CCACGG
HhaI 112 GCGC GCG^C
219 GCGC
HinP1I 112 GCGC G^CGC
219 GCGC
HincII 574 GTTAAC GT[CT]^[GA]AC
1348 GTTAAC
HindII 574 GTTAAC GT[CT]^[GA]AC
1348 GTTAAC
HpaI 574 GTTAAC GTT^AAC
1348 GTTAAC
HphI 243 GGTGA GGTGA(8/7)
276 GGTGA
MaeI 223 CTAG C^TAG
1060 CTAG
MseI 575 TTAA T^TAA
1349 TTAA
PflMI 861 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1249 CCAAGTCCTGG
PpuMI 422 GGGACCT [GA]G^G[AT]CC[CT]
1309 AGGACCC
PstI 316 CTGCAG CTGCA^G
1305 CTGCAG
Tru9I 575 TTAA T^TAA
1349 TTAA
Tsp45I 277 GTGAC ^GT[GC]AC
605 GTGAC
Tsp509I 209 AATT ^AATT
284 AATT
Van91I 861 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1249 CCAAGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TfiI TseI
TspRI TthHB8I XcmI XhoII