(Circular) (Six-base) MAPSORT of: pFN21AB9428.seq Check: 5520 from: 1 to: 6259 pFN21AB9428 6259 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2616 4069 278 Size: 53 83 186 936 1080 1453 2468 Fragments arranged by size: 2468 1453 1080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2616 3602 1937 Size: 679 986 4594 Fragments arranged by size: 4594 986 679 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2798 3153 3534 4322 5706 5974 1632 Size: 1166 355 381 788 1384 268 1917 Fragments arranged by size: 1917 1384 1166 788 381 355 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 5158 6135 1203 Size: 3955 977 1327 Fragments arranged by size: 3955 1327 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6259 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6259 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3241 3273 3241 Size: 32 6227 AlwNI CAG_nnn'CTG Cuts at: 4002 5574 4002 Size: 1572 4687 ApaLI G'TGCA_C Cuts at: 4316 5472 4316 Size: 1156 5103 ApoI r'AATT_y Cuts at: 1493 2213 2288 3568 3794 3946 6055 1493 Size: 720 75 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 720 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 3100 3267 3584 1870 Size: 73 1157 167 317 4545 Fragments arranged by size: 4545 1157 317 167 73 BamHI G'GATC_C Cuts at: 2628 3589 3956 2628 Size: 961 367 4931 Fragments arranged by size: 4931 961 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2546 3442 3580 5035 Size: 325 217 627 246 513 896 138 1455 Cuts at: 5035 618 Size: 1842 Fragments arranged by size: 1842 1455 896 627 513 325 246 217 138 BanII G_rGCy'C Cuts at: 1391 2082 2814 3578 1391 Size: 691 732 764 4072 Fragments arranged by size: 4072 764 732 691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2854 928 Size: 971 955 4333 Fragments arranged by size: 4333 971 955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4450 4484 1903 Size: 34 2513 34 3678 Fragments arranged by size: 3678 2513 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 1548 Size: 6259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4870 136 Size: 107 122 71 631 605 3198 1525 Fragments arranged by size: 3198 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6254 6254 Size: 6259 BlpI GC'TnA_GC Cuts at: 2733 3676 2733 Size: 943 5316 Bme1580I G_kGCm'C Cuts at: 1790 2038 2336 2549 3127 4320 5476 1790 Size: 248 298 213 578 1193 1156 2573 Fragments arranged by size: 2573 1193 1156 578 298 248 213 BmgBI CAC'GTC Cuts at: 1318 2470 1318 Size: 1152 5107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2263 2323 3990 Size: 416 53 83 399 24 846 60 1667 Cuts at: 3990 4948 442 Size: 958 1753 Fragments arranged by size: 1753 1667 958 846 416 399 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 2320 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2821 3183 3541 4919 1239 Size: 848 734 362 358 1378 2579 Fragments arranged by size: 2579 1378 848 734 362 358 Bpu10I CC'TnA_GC Cuts at: 2927 2927 Size: 6259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 4384 5249 5547 2417 Size: 1967 865 298 3129 Fragments arranged by size: 3129 1967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3197 4922 882 Size: 617 1698 1725 2219 Fragments arranged by size: 2219 1725 1698 617 BsaAI yAC'GTr Cuts at: 493 1204 4050 493 Size: 711 2846 2702 Fragments arranged by size: 2846 2702 711 BsaBI GATnn'nnATC Cuts at: 6253 6253 Size: 6259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2613 4066 4448 5083 Size: 53 83 186 936 1080 1453 382 635 Cuts at: 5083 275 Size: 1451 Fragments arranged by size: 1453 1451 1080 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2589 4692 5090 5364 5511 5922 2589 Size: 2103 398 274 147 411 2926 Fragments arranged by size: 2926 2103 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 1178 Size: 30 165 30 1407 30 4597 Fragments arranged by size: 4597 1407 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3083 3130 3146 1186 Size: 1897 47 16 4299 Fragments arranged by size: 4299 1897 47 16 BseYI C'CCAG_C Cuts at: 2027 3077 3230 5462 5958 2027 Size: 1050 153 2232 496 2328 Fragments arranged by size: 2328 2232 1050 496 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 2405 2450 1163 Size: 573 645 24 45 4972 Fragments arranged by size: 4972 645 573 45 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3347 3709 4470 5498 1540 Size: 275 186 1346 362 761 1028 2301 Fragments arranged by size: 2301 1346 1028 761 362 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2418 4320 4405 5476 2082 Size: 336 1902 85 1071 2865 Fragments arranged by size: 2865 1902 1071 336 85 BsmI GAATG_Cn' Cuts at: 2843 2893 3770 3863 2843 Size: 50 877 93 5239 Fragments arranged by size: 5239 877 93 50 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2082 2336 2418 2549 2814 3127 Size: 399 248 44 254 82 131 265 313 Cuts at: 3127 3578 4320 4405 5476 1391 Size: 451 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 399 313 265 254 248 131 85 82 44 BspEI T'CCGG_A Cuts at: 5922 5922 Size: 6259 BspHI T'CATG_A Cuts at: 4148 5878 4148 Size: 1730 4529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 3127 3614 844 Size: 1559 724 487 3489 Fragments arranged by size: 3489 1559 724 487 BsrBI CCG'CTC Cuts at: 1394 1823 2350 3349 4146 1394 Size: 429 527 999 797 3507 Fragments arranged by size: 3507 999 797 527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4754 4928 65 Size: 1183 3506 174 1396 Fragments arranged by size: 3506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2272 2718 4903 5090 1700 Size: 110 462 446 2185 187 2869 Fragments arranged by size: 2869 2185 462 446 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6259 BssSI C'ACGA_G Cuts at: 2687 4319 5331 2687 Size: 1632 1012 3615 Fragments arranged by size: 3615 1632 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2648 794 Size: 1854 4405 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6259 BstXI CCAn_nnnn'nTGG Cuts at: 3150 3537 3150 Size: 387 5872 BstYI r'GATC_y Cuts at: 1972 2263 2628 3508 3589 3956 4341 4358 5810 Size: 291 365 880 81 367 385 17 1452 Cuts at: 5810 5896 5908 6254 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 880 385 367 365 346 291 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 2901 513 Size: 552 1128 708 3871 Fragments arranged by size: 3871 1128 708 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5520 Fragments arranged by size: 5520 683 56 BtsI GCAGTG_nn' Cuts at: 2649 2706 2812 3775 4569 4589 2649 Size: 57 106 963 794 20 4319 Fragments arranged by size: 4319 963 794 106 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6224 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5266 809 Size: 507 3950 1802 Fragments arranged by size: 3950 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2345 3706 4595 Size: 54 1425 50 275 18 515 1361 889 Cuts at: 4595 6224 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1361 889 515 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3706 1537 Size: 275 1894 4090 Fragments arranged by size: 4090 1894 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2650 4189 1353 Size: 1297 1539 3423 Fragments arranged by size: 3423 1539 1297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4846 5220 5366 387 Size: 1064 138 3257 374 146 1280 Fragments arranged by size: 3257 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2080 2080 Size: 6259 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2798 2821 3153 3183 3534 3541 Size: 393 455 711 23 332 30 351 7 Cuts at: 3541 4322 4919 5706 5974 1239 Size: 781 597 787 268 1524 Fragments arranged by size: 1524 787 781 711 597 455 393 351 332 268 30 23 7 EcoNI CCTnn'n_nnAGG Cuts at: 3523 3523 Size: 6259 EcoO109I rG'GnC_Cy Cuts at: 2428 3315 3500 2428 Size: 887 185 5187 Fragments arranged by size: 5187 887 185 EcoRI G'AATT_C Cuts at: 2213 3568 3946 2213 Size: 1355 378 4526 Fragments arranged by size: 4526 1355 378 EcoRV GAT'ATC Cuts at: 2175 2175 Size: 6259 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2396 2396 Size: 6259 FspI TGC'GCA Cuts at: 4765 4765 Size: 6259 HaeII r_GCGC'y Cuts at: 1678 5406 1678 Size: 3728 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2810 2842 2903 2935 2937 Size: 32 135 32 1238 32 61 32 2 Cuts at: 2937 2969 4900 4932 4974 5006 1373 Size: 32 1931 32 42 32 2626 Fragments arranged by size: 2626 1931 1238 135 61 42 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3355 3603 3849 669 Size: 988 281 643 774 248 246 3079 Fragments arranged by size: 3079 988 774 643 281 248 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6259 HpaI GTT'AAC Cuts at: 2581 3355 3849 2581 Size: 774 494 4991 Fragments arranged by size: 4991 774 494 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 3364 5373 5557 1305 Size: 1161 898 2009 184 2007 Fragments arranged by size: 2009 2007 1161 898 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4437 Fragments arranged by size: 4437 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4217 4576 4735 518 Size: 648 3051 359 159 2042 Fragments arranged by size: 3051 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 3675 4352 5500 5745 1606 Size: 425 1644 677 1148 245 2120 Fragments arranged by size: 2120 1644 1148 677 425 245 NaeI GCC'GGC Cuts at: 2274 2274 Size: 6259 NcoI C'CATG_G Cuts at: 513 1065 2193 513 Size: 552 1128 4579 Fragments arranged by size: 4579 1128 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NgoMIV G'CCGG_C Cuts at: 2272 2272 Size: 6259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 3617 5162 1132 Size: 97 2388 1545 2229 Fragments arranged by size: 2388 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 5056 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 2872 3260 1108 Size: 1764 388 4107 Fragments arranged by size: 4107 1764 388 PfoI T'CCnGG_A Cuts at: 2832 3259 2832 Size: 427 5832 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2670 2702 4328 4360 5841 5873 2670 Size: 32 1626 32 1481 32 3056 Fragments arranged by size: 3056 1626 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 3315 2428 Size: 887 5372 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6259 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PstI C_TGCA'G Cuts at: 830 2325 3314 3611 830 Size: 1495 989 297 3478 Fragments arranged by size: 3478 1495 989 297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 SacI G_AGCT'C Cuts at: 2082 2082 Size: 6259 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SbfI CC_TGCA'GG Cuts at: 3314 3611 3314 Size: 297 5962 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SfcI C'TryA_G Cuts at: 826 1045 2321 2617 3310 3607 4742 5423 5614 Size: 219 1276 296 693 297 1135 681 191 Cuts at: 5614 826 Size: 1471 Fragments arranged by size: 1471 1276 1135 693 681 297 296 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 3586 1872 Size: 1714 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 4363 5264 5526 820 Size: 197 926 489 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 489 262 197 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 3617 1132 Size: 2485 3774 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 5179 StyI C'CwwG_G Cuts at: 513 1065 2046 2193 2814 2968 3698 513 Size: 552 981 147 621 154 730 3074 Fragments arranged by size: 3074 981 730 621 552 154 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4286 4303 4456 4641 5086 325 Size: 1255 2706 17 153 185 445 1498 Fragments arranged by size: 2706 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4505 6217 96 Size: 274 80 33 51 1443 2528 1712 138 Fragments arranged by size: 2528 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6219 6251 6219 Size: 32 6227 Tth111I GACn'n_nGTC Cuts at: 1316 2527 1316 Size: 1211 5048 XbaI T'CTAG_A Cuts at: 3064 3595 3064 Size: 531 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2200 2275 2449 3436 2062 Size: 138 75 174 987 4885 Fragments arranged by size: 4885 987 174 138 75 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6259 XmaI C'CCGG_G Cuts at: 1870 3584 1870 Size: 1714 4545 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2614 4067 276 Size: 53 83 186 936 1080 1453 2468 Fragments arranged by size: 2468 1453 1080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI