(Linear) (Six-base) MAPSORT of: KIBB9428.seq Check: 9439 from: 1 to: 1554 KIBB9428.orf With 182 enzymes: SgfI * July 3, 2012 16:37 .. AatII G_ACGT'C Cuts at: 0 611 1554 Size: 611 943 AccI GT'mk_AC Cuts at: 0 611 1554 Size: 611 943 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 1148 1529 1554 Size: 793 355 381 25 Fragments arranged by size: 793 381 355 25 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1236 1268 1554 Size: 1236 32 286 Fragments arranged by size: 1236 286 32 ApoI r'AATT_y Cuts at: 0 208 283 1554 Size: 208 75 1271 Fragments arranged by size: 1271 208 75 AvaI C'yCGr_G Cuts at: 0 1095 1262 1554 Size: 1095 167 292 Fragments arranged by size: 1095 292 167 BamHI G'GATC_C Cuts at: 0 623 1554 Size: 623 931 BanI G'GyrC_C Cuts at: 0 28 541 1437 1554 Size: 28 513 896 117 Fragments arranged by size: 896 513 117 28 BanII G_rGCy'C Cuts at: 0 77 809 1554 Size: 77 732 745 Fragments arranged by size: 745 732 77 BbsI GAAGACnn'nnnn_ Cuts at: 0 849 1554 Size: 849 705 BlpI GC'TnA_GC Cuts at: 0 728 1554 Size: 728 826 Bme1580I G_kGCm'C Cuts at: 0 33 331 544 1122 1554 Size: 33 298 213 578 432 Fragments arranged by size: 578 432 298 213 33 BmgBI CAC'GTC Cuts at: 0 465 1554 Size: 465 1089 BmrI ACTGGGnnnn_n' Cuts at: 0 258 318 1554 Size: 258 60 1236 Fragments arranged by size: 1236 258 60 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 315 1554 Size: 315 1239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 816 1178 1536 1554 Size: 82 734 362 358 18 Fragments arranged by size: 734 362 358 82 18 Bpu10I CC'TnA_GC Cuts at: 0 922 1554 Size: 922 632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 1554 Size: 412 1142 BsaI GGTCTCn'nnnn_ Cuts at: 0 1192 1554 Size: 1192 362 BsaHI Gr'CG_yC Cuts at: 0 608 1554 Size: 608 946 BsaWI w'CCGG_w Cuts at: 0 584 1554 Size: 584 970 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 805 835 1554 Size: 805 30 719 Fragments arranged by size: 805 719 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1078 1125 1141 1554 Size: 1078 47 16 413 Fragments arranged by size: 1078 413 47 16 BseYI C'CCAG_C Cuts at: 0 22 1072 1225 1554 Size: 22 1050 153 329 Fragments arranged by size: 1050 329 153 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 400 445 1554 Size: 376 24 45 1109 Fragments arranged by size: 1109 376 45 24 BsiEI CG_ry'CG Cuts at: 0 1342 1554 Size: 1342 212 BsiHKAI G_wGCw'C Cuts at: 0 77 413 1554 Size: 77 336 1141 Fragments arranged by size: 1141 336 77 BsmI GAATG_Cn' Cuts at: 0 838 888 1554 Size: 838 50 666 Fragments arranged by size: 838 666 50 Bsp1286I G_dGCh'C Cuts at: 0 33 77 331 413 544 809 1122 1554 Size: 33 44 254 82 131 265 313 432 Fragments arranged by size: 432 313 265 254 131 82 44 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 398 1122 1554 Size: 398 724 432 Fragments arranged by size: 724 432 398 BsrBI CCG'CTC Cuts at: 0 345 1344 1554 Size: 345 999 210 Fragments arranged by size: 999 345 210 BsrFI r'CCGG_y Cuts at: 0 267 713 1554 Size: 267 446 841 Fragments arranged by size: 841 446 267 BssSI C'ACGA_G Cuts at: 0 682 1554 Size: 682 872 BstAPI GCAn_nnn'nTGC Cuts at: 0 643 1554 Size: 643 911 BstXI CCAn_nnnn'nTGG Cuts at: 0 1145 1532 1554 Size: 1145 387 22 Fragments arranged by size: 1145 387 22 BstYI r'GATC_y Cuts at: 0 258 623 1503 1554 Size: 258 365 880 51 Fragments arranged by size: 880 365 258 51 BtgI C'CryG_G Cuts at: 0 188 896 1554 Size: 188 708 658 Fragments arranged by size: 708 658 188 BtsI GCAGTG_nn' Cuts at: 0 644 701 807 1554 Size: 644 57 106 747 Fragments arranged by size: 747 644 106 57 EaeI y'GGCC_r Cuts at: 0 340 1554 Size: 340 1214 EarI CTCTTCn'nnn_ Cuts at: 0 645 1554 Size: 645 909 EcoICRI GAG'CTC Cuts at: 0 75 1554 Size: 75 1479 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 793 816 1148 1178 1529 1536 1554 Size: 82 711 23 332 30 351 7 18 Fragments arranged by size: 711 351 332 82 30 23 18 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 1518 1554 Size: 1518 36 EcoO109I rG'GnC_Cy Cuts at: 0 423 1310 1495 1554 Size: 423 887 185 59 Fragments arranged by size: 887 423 185 59 EcoRI G'AATT_C Cuts at: 0 208 1554 Size: 208 1346 EcoRV GAT'ATC Cuts at: 0 170 1554 Size: 170 1384 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 391 1554 Size: 391 1163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 805 837 898 930 932 964 1554 Size: 805 32 61 32 2 32 590 Fragments arranged by size: 805 590 61 32 32 32 2 HincII GTy'rAC Cuts at: 0 576 1350 1554 Size: 576 774 204 Fragments arranged by size: 774 576 204 HpaI GTT'AAC Cuts at: 0 576 1350 1554 Size: 576 774 204 Fragments arranged by size: 774 576 204 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 461 1359 1554 Size: 461 898 195 Fragments arranged by size: 898 461 195 MspA1I CmG'CkG Cuts at: 0 26 1554 Size: 26 1528 NaeI GCC'GGC Cuts at: 0 269 1554 Size: 269 1285 NcoI C'CATG_G Cuts at: 0 188 1554 Size: 188 1366 NgoMIV G'CCGG_C Cuts at: 0 267 1554 Size: 267 1287 PasI CC'CwG_GG Cuts at: 0 546 1554 Size: 546 1008 PflMI CCAn_nnn'nTGG Cuts at: 0 867 1255 1554 Size: 867 388 299 Fragments arranged by size: 867 388 299 PfoI T'CCnGG_A Cuts at: 0 827 1254 1554 Size: 827 427 300 Fragments arranged by size: 827 427 300 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 665 697 1554 Size: 665 32 857 Fragments arranged by size: 857 665 32 PpuMI rG'GwC_Cy Cuts at: 0 423 1310 1554 Size: 423 887 244 Fragments arranged by size: 887 423 244 PstI C_TGCA'G Cuts at: 0 320 1309 1554 Size: 320 989 245 Fragments arranged by size: 989 320 245 SacI G_AGCT'C Cuts at: 0 77 1554 Size: 77 1477 SbfI CC_TGCA'GG Cuts at: 0 1309 1554 Size: 1309 245 SfcI C'TryA_G Cuts at: 0 316 612 1305 1554 Size: 316 296 693 249 Fragments arranged by size: 693 316 296 249 SmlI C'TyrA_G Cuts at: 0 427 1554 Size: 427 1127 StuI AGG'CCT Cuts at: 0 592 1554 Size: 592 962 StyI C'CwwG_G Cuts at: 0 41 188 809 963 1554 Size: 41 147 621 154 591 Fragments arranged by size: 621 591 154 147 41 Tth111I GACn'n_nGTC Cuts at: 0 522 1554 Size: 522 1032 XbaI T'CTAG_A Cuts at: 0 1059 1554 Size: 1059 495 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 57 195 270 444 1431 1554 Size: 57 138 75 174 987 123 Fragments arranged by size: 987 174 138 123 75 57 ZraI GAC'GTC Cuts at: 0 609 1554 Size: 609 945 Enzymes that do cut: AatII AccI AcuI AloI ApoI AvaI BamHI BanI BanII BbsI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HpaI MmeI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI PstI SacI SbfI SfcI SmlI StuI StyI Tth111I XbaI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmtI BsaAI BsaBI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI MscI MslI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI XhoI XmaI XmnI