################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BanII BbeI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI NarI NcoI NheI NotI NruI NsiI NspV PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 856 CGCG CG^CG AcsI 844 AAATTT [GA]^AATT[CT] AfaI 1287 GTAC GT^AC AflIII 468 ACGTGT A^C[GA][CT]GT Alw44I 859 GTGCAC G^TGCAC AlwNI 578 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 859 GTGCAC G^TGCAC ApoI 844 AAATTT [GA]^AATT[CT] AspI 1330 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1329 GGACC G^G[AT]CC BalI 241 TGGCCA TGG^CCA BamHI 1534 GGATCC G^GATCC BanI 1484 GGCACC G^G[CT][GA]CC BbrPI 467 CACGTG CAC^GTG BbvCI 1417 GCTGAGG CCTCAGC(-5/-2) BcgI 536 CGAGGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 918 TGATCA T^GATCA BcnI 1509 CCGGG CC^[GC]GG BglII 768 AGATCT A^GATCT BsaAI 467 CACGTG [CT]AC^GT[GA] BsaI 1433 GAGACC GGTCTC(1/5) BseRI 596 GAGGAG GAGGAG(10/8) Bsp1407I 1286 TGTACA T^GTACA BsrFI 76 GCCGGC [GA]^CCGG[CT] BsrGI 1286 TGTACA T^GTACA BstAPI 1485 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 856 CGCG CG^CG Cfr10I 76 GCCGGC [GA]^CCGG[CT] Cfr13I 1329 GGACC G^G[ACGT]CC EarI 331 GAAGAG CTCTTC(1/4) FbaI 918 TGATCA T^GATCA HincII 655 GTTGAC GT[CT]^[GA]AC HindII 655 GTTGAC GT[CT]^[GA]AC HindIII 761 AAGCTT A^AGCTT Ksp632I 331 GAAGAG CTCTTC(1/4) MluNI 241 TGGCCA TGG^CCA MscI 241 TGGCCA TGG^CCA MvnI 856 CGCG CG^CG NaeI 76 GCCGGC GCC^GGC NciI 1509 CCGGG CC^[GC]GG NdeI 914 CATATG CA^TATG NgoMIV 76 GCCGGC G^CCGGC NspI 462 ACATGC [GA]CATG^[CT] PacI 454 TTAATTAA TTAAT^TAA PflFI 1330 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1043 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 658 GACTC GAGTC(4/5) PmaCI 467 CACGTG CAC^GTG PmlI 467 CACGTG CAC^GTG RsaI 1287 GTAC GT^AC Sau96I 1329 GGACC G^G[ACGT]CC SexAI 1331 ACCAGGT A^CC[AT]GGT SfcI 908 CTGTAG C^T[GA][CT]AG SspBI 1286 TGTACA T^GTACA TfiI 999 GATTC G^A[AT]TC Tth111I 1330 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1043 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 973 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Asp700I 892 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 994 GAAGTGATTC AvaI 102 CTCGGG C^[CT]CG[GA]G 116 CTCGGG BbsI 524 GAAGAC GAAGAC(2/6) 223 GTCTTC BbvI 128 GCAGC GCAGC(8/12) 143 GCAGC BfaI 742 CTAG C^TAG 974 CTAG BpuAI 524 GAAGAC GAAGAC(2/6) 223 GTCTTC BsmFI 119 GGGAC GGGAC(10/14) 1215 GTCCC BsoBI 102 CTCGGG C^[CT]CG[GA]G 116 CTCGGG BspMI 530 ACCTGC ACCTGC(4/8) 546 GCAGGT BsrDI 1347 CATTGC GCAATG(2/0) 1490 CATTGC DraI 441 TTTAAA TTT^AAA 873 TTTAAA FauI 506 CCCGC CCCGC(4/6) 854 CCCGC Fnu4HI 128 GCAGC GC^[ACGT]GC 143 GCAGC HapII 77 CCGG C^CGG 1509 CCGG HinfI 658 GACTC G^A[ACGT]TC 999 GATTC HpaII 77 CCGG C^CGG 1509 CCGG HphI 1093 GGTGA GGTGA(8/7) 59 TCACC ItaI 128 GCAGC GC^[ACGT]GC 143 GCAGC MaeI 742 CTAG C^TAG 974 CTAG MspI 77 CCGG C^CGG 1509 CCGG NlaIV 1484 GGCACC GG[ACGT]^[ACGT]CC 1534 GGATCC SfaNI 1414 GATGC GCATC(5/9) 107 GCATC TaqI 1233 TCGA T^CGA 1464 TCGA TseI 128 GCAGC G^C[AT]GC 143 GCAGC Tsp45I 58 GTCAC ^GT[GC]AC 265 GTGAC TthHB8I 1233 TCGA T^CGA 1464 TCGA XcmI 1346 CCATTGCGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1471 CCAATAACGCACTGG XmnI 892 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 994 GAAGTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AlwI AspHI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EaeI EcoRII FokI HaeIII MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI Tru9I Tsp509I TspRI XhoII