(Circular) (Six-base) MAPSORT of: pFN21AE0999.seq Check: 6668 from: 1 to: 6274 pFN21AE0999 6274 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2544 2544 Size: 6274 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3595 3595 Size: 6274 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2355 3194 4337 5721 5989 1632 Size: 708 15 839 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1143 839 708 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 2473 5173 6150 1203 Size: 1270 2700 977 1327 Fragments arranged by size: 2700 1327 1270 977 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6274 AlwNI CAG_nnn'CTG Cuts at: 2588 4017 5589 2588 Size: 1429 1572 3273 Fragments arranged by size: 3273 1572 1429 ApaLI G'TGCA_C Cuts at: 2864 4331 5487 2864 Size: 1467 1156 3651 Fragments arranged by size: 3651 1467 1156 ApoI r'AATT_y Cuts at: 1493 2849 3583 3809 3961 6070 1493 Size: 1356 734 226 152 2109 1697 Fragments arranged by size: 2109 1697 1356 734 226 152 AseI AT'TA_AT Cuts at: 160 1032 4828 160 Size: 872 3796 1606 Fragments arranged by size: 3796 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2121 3599 1870 Size: 73 164 14 1478 4545 Fragments arranged by size: 4545 1478 164 73 14 BamHI G'GATC_C Cuts at: 3539 3604 3971 3539 Size: 65 367 5842 Fragments arranged by size: 5842 367 65 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3489 3595 5050 618 Size: 325 217 627 1702 106 1455 1842 Fragments arranged by size: 1842 1702 1455 627 325 217 106 BanII G_rGCy'C Cuts at: 1391 3593 1391 Size: 2202 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2221 2536 928 Size: 971 322 315 4666 Fragments arranged by size: 4666 971 322 315 BbvCI CC'TCA_GC Cuts at: 3423 3423 Size: 6274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2530 2564 4465 4499 1903 Size: 34 593 34 1901 34 3678 Fragments arranged by size: 3678 1901 593 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 2923 1548 Size: 1375 4899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4885 136 Size: 107 122 71 631 605 3213 1525 Fragments arranged by size: 3213 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2773 6269 2773 Size: 3496 2778 BlpI GC'TnA_GC Cuts at: 3691 3691 Size: 6274 Bme1580I G_kGCm'C Cuts at: 1790 2582 2868 4335 5491 1790 Size: 792 286 1467 1156 2573 Fragments arranged by size: 2573 1467 1156 792 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4005 4963 442 Size: 416 53 83 399 24 2588 958 1753 Fragments arranged by size: 2588 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6274 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2705 3022 3306 3521 4934 1239 Size: 1466 317 284 215 1413 2579 Fragments arranged by size: 2579 1466 1413 317 284 215 Bpu10I CC'TnA_GC Cuts at: 3423 3423 Size: 6274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4399 5264 5562 4399 Size: 865 298 5111 Fragments arranged by size: 5111 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3432 4937 882 Size: 617 1933 1505 2219 Fragments arranged by size: 2219 1933 1505 617 BsaAI yAC'GTr Cuts at: 493 1204 2474 4065 493 Size: 711 1270 1591 2702 Fragments arranged by size: 2702 1591 1270 711 BsaBI GATnn'nnATC Cuts at: 6268 6268 Size: 6274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4081 4463 5098 275 Size: 53 83 186 936 2548 382 635 1451 Fragments arranged by size: 2548 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6049 Fragments arranged by size: 6049 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2616 1186 Size: 1430 4844 BseYI C'CCAG_C Cuts at: 2425 3134 5477 5973 2425 Size: 709 2343 496 2726 Fragments arranged by size: 2726 2343 709 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 3184 3424 1163 Size: 573 303 1145 240 4013 Fragments arranged by size: 4013 1145 573 303 240 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2314 2868 4335 4420 5491 2108 Size: 206 554 1467 85 1071 2891 Fragments arranged by size: 2891 1467 1071 554 206 85 BsmI GAATG_Cn' Cuts at: 3785 3878 3785 Size: 93 6181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2314 2582 2868 3593 4335 4420 Size: 399 318 206 268 286 725 742 85 Cuts at: 4420 5491 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 725 399 318 286 268 206 85 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2542 2544 3629 844 Size: 1698 2 1085 3489 Fragments arranged by size: 3489 1698 1085 2 BsrBI CCG'CTC Cuts at: 1394 1823 4161 1394 Size: 429 2338 3507 Fragments arranged by size: 3507 2338 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3351 3494 4769 4943 65 Size: 1183 2103 143 1275 174 1396 Fragments arranged by size: 2103 1396 1275 1183 174 143 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 4918 5105 1700 Size: 110 271 2837 187 2869 Fragments arranged by size: 2869 2837 271 187 110 BsrGI T'GTAC_A Cuts at: 96 3291 96 Size: 3195 3079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 3496 794 Size: 2702 3572 BstBI TT'CG_AA Cuts at: 5078 5078 Size: 6274 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstYI r'GATC_y Cuts at: 1972 2773 3202 3539 3604 3971 4356 4373 5825 Size: 801 429 337 65 367 385 17 1452 Cuts at: 5825 5911 5923 6269 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 801 429 385 367 346 337 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5722 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3194 487 Size: 683 56 1968 3567 Fragments arranged by size: 3567 1968 683 56 BtsI GCAGTG_nn' Cuts at: 3790 4584 4604 3790 Size: 794 20 5460 Fragments arranged by size: 5460 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2680 2715 529 Size: 35 2116 35 4088 Fragments arranged by size: 4088 2116 35 35 DraI TTT'AAA Cuts at: 2448 2880 3578 2448 Size: 432 698 5144 Fragments arranged by size: 5144 698 432 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2246 3511 Size: 54 1425 50 275 18 253 163 1265 Cuts at: 3511 3721 4610 6239 8 Size: 210 889 1629 43 Fragments arranged by size: 1629 1425 1265 889 275 253 210 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 4204 1353 Size: 978 1873 3423 Fragments arranged by size: 3423 1873 978 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2157 4861 5235 5381 387 Size: 1064 138 568 2704 374 146 1280 Fragments arranged by size: 2704 1280 1064 568 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2355 2705 3022 3194 3306 3521 Size: 393 708 15 350 317 172 112 215 Cuts at: 3521 4337 4934 5721 5989 1239 Size: 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 708 597 393 350 317 268 215 172 112 15 EcoRI G'AATT_C Cuts at: 3583 3961 3583 Size: 378 5896 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3023 3023 Size: 6274 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 5421 1678 Size: 3743 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2486 2518 2866 2898 4915 Size: 32 135 32 914 32 348 32 2017 Cuts at: 4915 4947 4989 5021 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2017 914 348 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 3618 3864 669 Size: 988 281 724 956 246 3079 Fragments arranged by size: 3079 988 956 724 281 246 HindIII A'AGCT_T Cuts at: 748 2766 748 Size: 2018 4256 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3599 3599 Size: 6274 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 5388 5572 1305 Size: 996 3087 184 2007 Fragments arranged by size: 3087 2007 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2248 10 Size: 54 1425 343 416 4036 Fragments arranged by size: 4036 1425 416 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2472 2952 3398 3447 4232 4591 4750 Size: 648 1306 480 446 49 785 359 159 Cuts at: 4750 518 Size: 2042 Fragments arranged by size: 2042 1306 785 648 480 446 359 159 49 MspA1I CmG'CkG Cuts at: 1606 3690 4367 5515 5760 1606 Size: 2084 677 1148 245 2120 Fragments arranged by size: 2120 2084 1148 677 245 NaeI GCC'GGC Cuts at: 2083 2083 Size: 6274 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5722 NdeI CA'TA_TG Cuts at: 387 2920 387 Size: 2533 3741 NgoMIV G'CCGG_C Cuts at: 2081 2081 Size: 6274 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6274 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2471 3632 5177 1132 Size: 97 1242 1161 1545 2229 Fragments arranged by size: 2229 1545 1242 1161 97 PacI TTA_AT'TAA Cuts at: 2463 2463 Size: 6274 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6274 PciI A'CATG_T Cuts at: 5173 5173 Size: 6274 PflMI CCAn_nnn'nTGG Cuts at: 1108 3054 1108 Size: 1946 4328 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PmlI CAC'GTG Cuts at: 2474 2474 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2577 2609 2577 Size: 32 6242 PstI C_TGCA'G Cuts at: 830 3626 830 Size: 2796 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 4522 6234 4522 Size: 1712 4562 SexAI A'CCwGG_T Cuts at: 3336 3336 Size: 6274 SfcI C'TryA_G Cuts at: 826 1045 2913 3622 4757 5438 5629 826 Size: 219 1868 709 1135 681 191 1471 Fragments arranged by size: 1868 1471 1135 709 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3601 1872 Size: 1729 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4378 5279 5541 820 Size: 197 926 2435 901 262 1553 Fragments arranged by size: 2435 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 3632 1132 Size: 2500 3774 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6274 StyI C'CwwG_G Cuts at: 513 1065 3713 513 Size: 552 2648 3074 Fragments arranged by size: 3074 2648 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3086 3507 4301 4318 4471 4656 5101 Size: 1255 1506 421 794 17 153 185 445 Cuts at: 5101 325 Size: 1498 Fragments arranged by size: 1506 1498 1255 794 445 421 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3291 4520 6232 Size: 274 80 33 51 1443 1314 1229 1712 Cuts at: 6232 96 Size: 138 Fragments arranged by size: 1712 1443 1314 1229 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2275 2307 6234 6266 2275 Size: 32 3927 32 2283 Fragments arranged by size: 3927 2283 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3338 1316 Size: 2022 4252 XbaI T'CTAG_A Cuts at: 2978 3610 2978 Size: 632 5642 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3358 3483 3358 Size: 125 6149 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6274 XmaI C'CCGG_G Cuts at: 1870 3599 1870 Size: 1729 4545 XmnI GAAnn'nnTTC Cuts at: 2901 3003 4403 2901 Size: 102 1400 4772 Fragments arranged by size: 4772 1400 102 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PfoI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI