################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AclI AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI BamHI BbrPI BcgI BclI BsaAI BsaBI BsiWI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI HindIII HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SfiI SfuI SnaBI SpeI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1129 GACGTC GACGT^C AccIII 3167 TCCGGA T^CCGGA AcsI 2847 AAATTC [GA]^AATT[CT] AflII 2653 CTTAAG C^TTAAG Alw44I 30 GTGCAC G^TGCAC Aor51HI 1760 AGCGCT AGC^GCT ApaLI 30 GTGCAC G^TGCAC ApoI 2847 AAATTC [GA]^AATT[CT] AviII 3029 TGCGCA TGC^GCA AvrII 2967 CCTAGG C^CTAGG BciVI 681 GGATAC GTATCC(6/5) BfrI 2653 CTTAAG C^TTAAG BglII 2312 AGATCT A^GATCT BlnI 2967 CCTAGG C^CTAGG BlpI 111 GCTGAGC GC^T[ACGT]AGC Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC BseAI 3167 TCCGGA T^CCGGA BsiEI 3221 CGGCCG CG[GA][CT]^CG BsmI 3109 GAATGC GAATGC(1/-1) BspEI 3167 TCCGGA T^CCGGA BstAPI 23 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2694 GGTCACC G^GT[ACGT]ACC BstPI 2694 GGTCACC G^GT[ACGT]ACC CelII 111 GCTGAGC GC^T[ACGT]AGC DrdI 1600 GACTGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3221 CGGCCG C^GGCCG EclXI 3221 CGGCCG C^GGCCG Eco47III 1760 AGCGCT AGC^GCT Eco52I 3221 CGGCCG C^GGCCG EcoO65I 2694 GGTCACC G^GT[ACGT]ACC FseI 761 GGCCGGCC GGCCGG^CC FspI 3029 TGCGCA TGC^GCA MroI 3167 TCCGGA T^CCGGA PshAI 2782 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 43 CCTGCAGG CCTGCA^GG SexAI 911 ACCTGGT A^CC[AT]GGT SgrAI 2748 CACCGGCG C[GA]^CCGG[CT]G SphI 1658 GCATGC GCATG^C Sse8387I 43 CCTGCAGG CCTGCA^GG StuI 509 AGGCCT AGG^CCT Tsp509I 2848 AATT ^AATT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 361 GTCTAC GT^[AC][GT]AC 1609 GTCTAC BsaI 856 GAGACC GGTCTC(1/5) 3229 GAGACC BsaWI 3167 TCCGGA [AT]^CCGG[AT] 3261 TCCGGT BsmBI 1326 CGTCTC CGTCTC(1/5) 249 GAGACG Bsp1407I 1682 TGTACA T^GTACA 1979 TGTACA BsrGI 1682 TGTACA T^GTACA 1979 TGTACA BssHII 2102 GCGCGC G^CGCGC 3116 GCGCGC DraIII 24 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG 3094 CACTGCGTG EcoNI 2967 CCTAGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3075 CCTCCACAAGG HincII 1287 GTCAAC GT[CT]^[GA]AC 2176 GTTGAC HindII 1287 GTCAAC GT[CT]^[GA]AC 2176 GTTGAC MseI 1745 TTAA T^TAA 2654 TTAA NcoI 59 CCATGG C^CATGG 3242 CCATGG PflMI 120 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3093 CCACTGCGTGG SapI 1638 GAAGAGC GCTCTTC(1/4) 3120 GCTCTTC SmlI 736 CTCAAG C^T[CT][GA]AG 2653 CTTAAG SspBI 1682 TGTACA T^GTACA 1979 TGTACA Tru9I 1745 TTAA T^TAA 2654 TTAA Van91I 120 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3093 CCACTGCGTGG XbaI 2790 TCTAGA T^CTAGA 3444 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI