Restriction map for
pFN21AE0998
(Circular) (Six-base) MAPSORT of: pFN21AE0998.seq Check: 4356 from: 1 to: 8203
pFN21AE0998 8203 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3658 5427 3658
Size: 1769 6434
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3138 6013 278
Size: 53 83 186 936 1602 2875 2468
Fragments arranged by size:
2875 2468 1602 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2367 3615 5546 1937
Size: 430 1248 1931 4594
Fragments arranged by size:
4594 1931 1248 430
Acc65I G'GTAC_C
Cuts at: 5524 5524
Size: 8203
AclI AA'CG_TT
Cuts at: 1563 6330 6703 1563
Size: 4767 373 3063
Fragments arranged by size:
4767 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2490 2981 3308 3399 5113 6266 7650
Size: 479 379 491 327 91 1714 1153 1384
Cuts at: 7650 7918 1632
Size: 268 1917
Fragments arranged by size:
1917 1714 1384 1153 491 479 379 327
268 91
AfeI AGC'GCT
Cuts at: 3767 3767
Size: 8203
AflII C'TTAA_G
Cuts at: 820 1017 4658 820
Size: 197 3641 4365
Fragments arranged by size:
4365 3641 197
AflIII A'CryG_T
Cuts at: 1203 7102 8079 1203
Size: 5899 977 1327
Fragments arranged by size:
5899 1327 977
AgeI A'CCGG_T
Cuts at: 7034 7034
Size: 8203
AhdI GACnn_n'nnGTC
Cuts at: 6932 6932
Size: 8203
AleI CACnn'nnGTG
Cuts at: 1166 2315 2898 1166
Size: 1149 583 6471
Fragments arranged by size:
6471 1149 583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4263 1184
Size: 3079 5124
AlwNI CAG_nnn'CTG
Cuts at: 2917 4342 5210 5946 7518 2917
Size: 1425 868 736 1572 3602
Fragments arranged by size:
3602 1572 1425 868 736
ApaI G_GGCC'C
Cuts at: 2233 4290 5313 2233
Size: 2057 1023 5123
Fragments arranged by size:
5123 2057 1023
ApaLI G'TGCA_C
Cuts at: 2035 6260 7416 2035
Size: 4225 1156 2822
Fragments arranged by size:
4225 2822 1156
ApoI r'AATT_y
Cuts at: 1493 4852 5512 5738 5890 7999 1493
Size: 3359 660 226 152 2109 1697
Fragments arranged by size:
3359 2109 1697 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6757 160
Size: 872 5725 1606
Fragments arranged by size:
5725 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8203
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2226 2357 2925 3059 3233 3278
Size: 73 244 39 131 568 134 174 45
Cuts at: 3278 3418 4102 4321 4381 4528 5528 1870
Size: 140 684 219 60 147 1000 4545
Fragments arranged by size:
4545 1000 684 568 244 219 174 147
140 134 131 73 60 45 39
AvrII C'CTAG_G
Cuts at: 4972 4972
Size: 8203
BamHI G'GATC_C
Cuts at: 5533 5900 5533
Size: 367 7836
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2068 2305 2435 2816
Size: 325 217 627 240 41 237 130 381
Cuts at: 2816 3117 3843 4136 4451 4469 4573 4757 5524
Size: 301 726 293 315 18 104 184 767
Cuts at: 5524 6979 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 767 726 627 381 325 315
301 293 240 237 217 184 130 104
41 18
BanII G_rGCy'C
Cuts at: 1391 2233 2421 2642 4290 4818 4949 4962 5019
Size: 842 188 221 1648 528 131 13 57
Cuts at: 5019 5313 5522 1391
Size: 294 209 4072
Fragments arranged by size:
4072 1648 842 528 294 221 209 188
131 57 13
BbeI G_GCGC'C
Cuts at: 2072 3121 3847 4140 4761 2072
Size: 1049 726 293 621 5514
Fragments arranged by size:
5514 1049 726 621 293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 2409 2969 3789 3827 928
Size: 971 354 156 560 820 38 5304
Fragments arranged by size:
5304 971 820 560 354 156 38
BbvCI CC'TCA_GC
Cuts at: 2022 2693 4495 2022
Size: 671 1802 5730
Fragments arranged by size:
5730 1802 671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6394 6428 1903
Size: 34 4457 34 3678
Fragments arranged by size:
4457 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2680 6095 7305 2680
Size: 3415 1210 3578
Fragments arranged by size:
3578 3415 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8203
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4280 4347 4553
Size: 107 122 71 631 605 2608 67 206
Cuts at: 4553 4643 4988 5219 6814 136
Size: 90 345 231 1595 1525
Fragments arranged by size:
2608 1595 1525 631 605 345 231 206
122 107 90 71 67
BglII A'GATC_T
Cuts at: 4317 8198 4317
Size: 3881 4322
BlpI GC'TnA_GC
Cuts at: 2117 5620 2117
Size: 3503 4700
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2039 2233 2440 2819 3216 3223 4290
Size: 240 9 194 207 379 397 7 1067
Cuts at: 4290 4454 5313 5337 6264 7420 1790
Size: 164 859 24 927 1156 2573
Fragments arranged by size:
2573 1156 1067 927 859 397 379 240
207 194 164 24 9 7
BmgBI CAC'GTC
Cuts at: 1318 3526 3699 3706 1318
Size: 2208 173 7 5815
Fragments arranged by size:
5815 2208 173 7
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4284 5934
Size: 416 53 83 399 24 1793 1074 1650
Cuts at: 5934 6892 442
Size: 958 1753
Fragments arranged by size:
1793 1753 1650 1074 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2536 2776 4618 2437
Size: 99 240 1842 6022
Fragments arranged by size:
6022 1842 240 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2462 2879 3047 4195 4557 5324 5471
Size: 1127 96 417 168 1148 362 767 147
Cuts at: 5471 6863 1239
Size: 1392 2579
Fragments arranged by size:
2579 1392 1148 1127 767 417 362 168
147 96
Bpu10I CC'TnA_GC
Cuts at: 2022 2693 4196 4217 4495 2022
Size: 671 1503 21 278 5730
Fragments arranged by size:
5730 1503 671 278 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2726 6328 7193 7491 2726
Size: 3602 865 298 3438
Fragments arranged by size:
3602 3438 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2855 5228 6866 882
Size: 617 1356 2373 1638 2219
Fragments arranged by size:
2373 2219 1638 1356 617
BsaAI yAC'GTr
Cuts at: 493 1204 5994 493
Size: 711 4790 2702
Fragments arranged by size:
4790 2702 711
BsaBI GATnn'nnATC
Cuts at: 8197 8197
Size: 8203
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2151 3118 3135
Size: 53 83 186 936 536 82 967 17
Cuts at: 3135 3844 4137 4758 6010 6392 7027 275
Size: 709 293 621 1252 382 635 1451
Fragments arranged by size:
1451 1252 967 936 709 635 621 536
382 293 186 83 82 53 17
BsaWI w'CCGG_w
Cuts at: 5172 5266 6636 7034 7308 7455 7866 5172
Size: 94 1370 398 274 147 411 5509
Fragments arranged by size:
5509 1370 411 398 274 147 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 2525 2555 2624
Size: 30 165 30 738 30 354 30 69
Cuts at: 2624 2654 3953 3983 4082 4112 4288 4318 4763
Size: 30 1299 30 99 30 176 30 445
Cuts at: 4763 4793 5227 5257 1178
Size: 30 434 30 4124
Fragments arranged by size:
4124 1299 738 445 434 354 176 165
99 69 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2429 2459 2509 2653 2738 2804 3974
Size: 1126 117 30 50 144 85 66 1170
Cuts at: 3974 4090 4172 5199 1186
Size: 116 82 1027 4190
Fragments arranged by size:
4190 1170 1126 1027 144 117 116 85
82 66 50 30
BseYI C'CCAG_C
Cuts at: 2489 2774 2840 3182 3224 3741 4111 4132 4283
Size: 285 66 342 42 517 370 21 151
Cuts at: 4283 4457 4705 4817 5318 5401 7406 7902 2489
Size: 174 248 112 501 83 2005 496 2790
Fragments arranged by size:
2790 2005 517 501 496 370 342 285
248 174 151 112 83 66 42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 2410 2615 3029 3838 1163
Size: 573 660 14 205 414 809 5528
Fragments arranged by size:
5528 809 660 573 414 205 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5229 5653 6414 7442 1540
Size: 275 186 3228 424 761 1028 2301
Fragments arranged by size:
3228 2301 1028 761 424 275 186
BsiHKAI G_wGCw'C
Cuts at: 2039 2999 3471 3650 3698 4155 4436 4754 6264
Size: 960 472 179 48 457 281 318 1510
Cuts at: 6264 6349 7420 2039
Size: 85 1071 2822
Fragments arranged by size:
2822 1510 1071 960 472 457 318 281
179 85 48
BsmI GAATG_Cn'
Cuts at: 5120 5714 5807 5120
Size: 594 93 7516
Fragments arranged by size:
7516 594 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2248 3337 2248
Size: 1089 7114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2039 2233 2421 2440 2642 2819
Size: 399 240 9 194 188 19 202 177
Cuts at: 2819 2999 3216 3223 3471 3650 3698 4155 4290
Size: 180 217 7 248 179 48 457 135
Cuts at: 4290 4436 4454 4754 4818 4949 4962 5019 5313
Size: 146 18 300 64 131 13 57 294
Cuts at: 5313 5337 5522 6264 6349 7420 1391
Size: 24 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 399 300 294 248
240 217 202 194 188 185 180 179
177 146 135 131 85 64 57 48
24 19 18 13 9 7
BspEI T'CCGG_A
Cuts at: 5172 7866 5172
Size: 2694 5509
BspHI T'CATG_A
Cuts at: 6092 7822 6092
Size: 1730 6473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3658 4687 5028 5040 5427 5558 844
Size: 2207 607 1029 341 12 387 131 3489
Fragments arranged by size:
3489 2207 1029 607 387 341 131 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2794 4098 5315 6090 1394
Size: 429 971 1304 1217 775 3507
Fragments arranged by size:
3507 1304 1217 971 775 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6698 6872 65
Size: 1183 5450 174 1396
Fragments arranged by size:
5450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2153 2302 2767 2966 4022 4086 4754
Size: 110 343 149 465 199 1056 64 668
Cuts at: 4754 6847 7034 1700
Size: 2093 187 2869
Fragments arranged by size:
2869 2093 1056 668 465 343 199 187
149 110 64
BsrGI T'GTAC_A
Cuts at: 96 3687 3984 96
Size: 3591 297 4315
Fragments arranged by size:
4315 3591 297
BssHII G'CGCG_C
Cuts at: 1925 4107 5121 1925
Size: 2182 1014 5007
Fragments arranged by size:
5007 2182 1014
BssSI C'ACGA_G
Cuts at: 3511 3691 3716 4154 6263 7275 3511
Size: 180 25 438 2109 1012 4439
Fragments arranged by size:
4439 2109 1012 438 180 25
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2034 794
Size: 1240 6963
BstBI TT'CG_AA
Cuts at: 7007 7007
Size: 8203
BstEII G'GTnAC_C
Cuts at: 1184 4699 1184
Size: 3515 4688
BstXI CCAn_nnnn'nTGG
Cuts at: 3434 3638 4465 3434
Size: 204 827 7172
Fragments arranged by size:
7172 827 204
BstYI r'GATC_y
Cuts at: 1972 2008 4317 4599 5397 5533 5900 6285 6302
Size: 36 2309 282 798 136 367 385 17
Cuts at: 6302 7754 7840 7852 8198 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2309 1977 1452 798 385 367 346 282
136 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 3321 3622 4426 4551 5247
Size: 552 939 60 1257 301 804 125 696
Cuts at: 5247 5283 5289 513
Size: 36 6 3427
Fragments arranged by size:
3427 1257 939 804 696 552 301 125
60 36 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2163 2409 3076 487
Size: 683 56 937 246 667 5614
Fragments arranged by size:
5614 937 683 667 246 56
BtsI GCAGTG_nn'
Cuts at: 2209 2268 2905 3475 3658 3835 4009 4266 5098
Size: 59 637 570 183 177 174 257 832
Cuts at: 5098 5719 6513 6533 2209
Size: 621 794 20 3879
Fragments arranged by size:
3879 832 794 637 621 570 257 183
177 174 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8168
DraI TTT'AAA
Cuts at: 5507 5507
Size: 8203
DraIII CAC_nnn'GTG
Cuts at: 2034 5104 2034
Size: 3070 5133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3611 7210 809
Size: 507 2295 3599 1802
Fragments arranged by size:
3599 2295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2604 2765 3436
Size: 54 1425 50 275 18 774 161 671
Cuts at: 3436 4423 4635 4834 5065 5226 5286 5650 6539
Size: 987 212 199 231 161 60 364 889
Cuts at: 6539 8168 8
Size: 1629 43
Fragments arranged by size:
1629 1425 987 889 774 671 364 275
231 212 199 161 161 60 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5226 5650 1537
Size: 275 3414 424 4090
Fragments arranged by size:
4090 3414 424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3056 3638 5132 6133 1353
Size: 1703 582 1494 1001 3423
Fragments arranged by size:
3423 1703 1494 1001 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2341 3162 3521 4180 4946 6790
Size: 1064 138 752 821 359 659 766 1844
Cuts at: 6790 7164 7310 387
Size: 374 146 1280
Fragments arranged by size:
1844 1280 1064 821 766 752 659 374
359 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2366 2462 2490 2879 2981 3047
Size: 393 479 255 96 28 389 102 66
Cuts at: 3047 3308 3399 4195 4557 5113 5324 5471 6266
Size: 261 91 796 362 556 211 147 795
Cuts at: 6266 6863 7650 7918 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 796 795 787 597 556 479 393
389 362 268 261 255 211 147 102
96 91 66 28
EcoNI CCTnn'n_nnAGG
Cuts at: 4976 5084 4976
Size: 108 8095
EcoO109I rG'GnC_Cy
Cuts at: 2230 2341 3158 4339 4615 4766 5309 2230
Size: 111 817 1181 276 151 543 5124
Fragments arranged by size:
5124 1181 817 543 276 151 111
EcoRI G'AATT_C
Cuts at: 5512 5890 5512
Size: 378 7825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2959 3313 3952 5110 2635
Size: 324 354 639 1158 5728
Fragments arranged by size:
5728 1158 639 354 324
FseI GG_CCGG'CC
Cuts at: 2771 2771
Size: 8203
FspI TGC'GCA
Cuts at: 5036 6709 5036
Size: 1673 6530
HaeII r_GCGC'y
Cuts at: 1678 2072 3121 3231 3769 3847 4087 4140 4224
Size: 394 1049 110 538 78 240 53 84
Cuts at: 4224 4761 4994 7350 1678
Size: 537 233 2356 2531
Fragments arranged by size:
2531 2356 1049 538 537 394 240 233
110 84 78 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 4048 4080 5227
Size: 32 135 32 569 32 1875 32 1147
Cuts at: 5227 5259 6844 6876 6918 6950 1373
Size: 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1875 1585 1147 569 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 4183 5547 5793 669
Size: 988 281 1356 889 1364 246 3079
Fragments arranged by size:
3079 1364 1356 988 889 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8203
HpaI GTT'AAC
Cuts at: 5793 5793
Size: 8203
KasI G'GCGC_C
Cuts at: 2068 3117 3843 4136 4757 2068
Size: 1049 726 293 621 5514
Fragments arranged by size:
5514 1049 726 621 293
KpnI G_GTAC'C
Cuts at: 5528 5528
Size: 8203
MfeI C'AATT_G
Cuts at: 5802 5802
Size: 8203
MluI A'CGCG_T
Cuts at: 8079 8079
Size: 8203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4401 7317 7501 1305
Size: 3096 2916 184 2007
Fragments arranged by size:
3096 2916 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2606 3438 4637 4836 10
Size: 54 1425 343 774 832 1199 199 3377
Fragments arranged by size:
3377 1425 1199 832 774 343 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2898 3636 3668 6161 6520 6679
Size: 648 1149 583 738 32 2493 359 159
Cuts at: 6679 518
Size: 2042
Fragments arranged by size:
2493 2042 1149 738 648 583 359 159
32
MspA1I CmG'CkG
Cuts at: 1606 2021 2484 3598 4652 4952 5368 5619 6296
Size: 415 463 1114 1054 300 416 251 677
Cuts at: 6296 7444 7689 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1114 1054 677 463 416 415
300 251 245
NaeI GCC'GGC
Cuts at: 2155 2304 2769 4024 4088 2155
Size: 149 465 1255 64 6270
Fragments arranged by size:
6270 1255 465 149 64
NarI GG'CG_CC
Cuts at: 2069 3118 3844 4137 4758 2069
Size: 1049 726 293 621 5514
Fragments arranged by size:
5514 1049 726 621 293
NcoI C'CATG_G
Cuts at: 513 1065 2004 2064 5247 513
Size: 552 939 60 3183 3469
Fragments arranged by size:
3469 3183 939 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8203
NgoMIV G'CCGG_C
Cuts at: 2153 2302 2767 4022 4086 2153
Size: 149 465 1255 64 6270
Fragments arranged by size:
6270 1255 465 149 64
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8203
NotI GC'GGCC_GC
Cuts at: 5650 5650
Size: 8203
NspI r_CATG'y
Cuts at: 1132 1229 2569 3547 3667 5561 7106 1132
Size: 97 1340 978 120 1894 1545 2229
Fragments arranged by size:
2229 1894 1545 1340 978 120 97
PasI CC'CwG_GG
Cuts at: 1348 2383 4336 1348
Size: 1035 1953 5215
Fragments arranged by size:
5215 1953 1035
PciI A'CATG_T
Cuts at: 7102 7102
Size: 8203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 5104 1108
Size: 1023 2973 4207
Fragments arranged by size:
4207 2973 1023
PfoI T'CCnGG_A
Cuts at: 5493 5493
Size: 8203
PmeI GTTT'AAAC
Cuts at: 5507 5507
Size: 8203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6272 6304 7785 7817 6272
Size: 32 1481 32 6658
Fragments arranged by size:
6658 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4791 1613
Size: 3178 5025
PsiI TTA'TAA
Cuts at: 5773 5773
Size: 8203
PspOMI G'GGCC_C
Cuts at: 2229 4286 5309 2229
Size: 2057 1023 5123
Fragments arranged by size:
5123 2057 1023
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2273 2305 2273
Size: 32 8171
PstI C_TGCA'G
Cuts at: 830 2053 3573 3611 3842 4651 5555 830
Size: 1223 1520 38 231 809 904 3478
Fragments arranged by size:
3478 1520 1223 904 809 231 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8203
PvuII CAG'CTG
Cuts at: 2021 4652 5368 2021
Size: 2631 716 4856
Fragments arranged by size:
4856 2631 716
SalI G'TCGA_C
Cuts at: 1936 5545 1936
Size: 3609 4594
SapI GCTCTTCn'nnn_
Cuts at: 3638 5132 3638
Size: 1494 6709
SbfI CC_TGCA'GG
Cuts at: 2053 5555 2053
Size: 3502 4701
ScaI AGT'ACT
Cuts at: 6451 8163 6451
Size: 1712 6491
SexAI A'CCwGG_T
Cuts at: 2916 2916
Size: 8203
SfcI C'TryA_G
Cuts at: 826 1045 2049 3569 3607 3838 4647 5551 6686
Size: 219 1004 1520 38 231 809 904 1135
Cuts at: 6686 7367 7558 826
Size: 681 191 1471
Fragments arranged by size:
1520 1471 1135 1004 904 809 681 231
219 191 38
SfoI GGC'GCC
Cuts at: 2070 3119 3845 4138 4759 2070
Size: 1049 726 293 621 5514
Fragments arranged by size:
5514 1049 726 621 293
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8203
SgrAI Cr'CCGG_yG
Cuts at: 1700 4754 1700
Size: 3054 5149
SmaI CCC'GGG
Cuts at: 1872 2189 2228 2927 3061 3235 5530 1872
Size: 317 39 699 134 174 2295 4545
Fragments arranged by size:
4545 2295 699 317 174 134 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2741 4658 6307 7208 7470 820
Size: 197 926 798 1917 1649 901 262 1553
Fragments arranged by size:
1917 1649 1553 926 901 798 262 197
SnaBI TAC'GTA
Cuts at: 493 5994 493
Size: 5501 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8203
SphI G_CATG'C
Cuts at: 1132 3667 5561 1132
Size: 2535 1894 3774
Fragments arranged by size:
3774 2535 1894
SrfI GCCC'GGGC
Cuts at: 2228 2927 2228
Size: 699 7504
SspI AAT'ATT
Cuts at: 5 52 6127 5
Size: 47 6075 2081
Fragments arranged by size:
6075 2081 47
StuI AGG'CCT
Cuts at: 1517 2516 1517
Size: 999 7204
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2424 2472 2865 3165 3927
Size: 552 939 60 360 48 393 300 762
Cuts at: 3927 4140 4972 5136 5247 5433 5642 513
Size: 213 832 164 111 186 209 3074
Fragments arranged by size:
3074 939 832 762 552 393 360 300
213 209 186 164 111 60 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 2323 2567 2801 3665 4457 4528
Size: 1255 686 57 244 234 864 792 71
Cuts at: 4528 6230 6247 6400 6585 7030 325
Size: 1702 17 153 185 445 1498
Fragments arranged by size:
1702 1498 1255 864 792 686 445 244
234 185 153 71 57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 2727 3687
Size: 274 80 33 51 1443 312 438 960
Cuts at: 3687 3984 6449 8161 96
Size: 297 2465 1712 138
Fragments arranged by size:
2465 1712 1443 960 438 312 297 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2624 2656 4082 4114 4145 4177 4288 4320 4763
Size: 32 1426 32 31 32 111 32 443
Cuts at: 4763 4795 8163 8195 2624
Size: 32 3368 32 2632
Fragments arranged by size:
3368 2632 1426 443 111 32 32 32
32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8203
XbaI T'CTAG_A
Cuts at: 4795 5449 5539 4795
Size: 654 90 7459
Fragments arranged by size:
7459 654 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2201 2317 3022 3184 4343 5245 2201
Size: 116 705 162 1159 902 5159
Fragments arranged by size:
5159 1159 902 705 162 116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8203
XmaI C'CCGG_G
Cuts at: 1870 2187 2226 2925 3059 3233 5528 1870
Size: 317 39 699 134 174 2295 4545
Fragments arranged by size:
4545 2295 699 317 174 134 39
XmnI GAAnn'nnTTC
Cuts at: 6332 6332
Size: 8203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3136 6011 276
Size: 53 83 186 936 1602 2875 2468
Fragments arranged by size:
2875 2468 1602 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI
EcoRV FspAI NruI NsiI PacI PmlI PpuMI PspXI
RsrII SacI SacII SanDI SfiI SwaI