(Linear) (Six-base) MAPSORT of: KIEE0998.seq Check: 3157 from: 1 to: 3498 KIEE0998.orf With 182 enzymes: SgfI * July 3, 2012 16:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1653 3422 3498 Size: 1653 1769 76 Fragments arranged by size: 1769 1653 76 AatII G_ACGT'C Cuts at: 0 1133 3498 Size: 1133 2365 AccI GT'mk_AC Cuts at: 0 362 1610 3498 Size: 362 1248 1888 Fragments arranged by size: 1888 1248 362 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 106 485 976 1303 1394 3108 3498 Size: 106 379 491 327 91 1714 390 Fragments arranged by size: 1714 491 390 379 327 106 91 AfeI AGC'GCT Cuts at: 0 1762 3498 Size: 1762 1736 AflII C'TTAA_G Cuts at: 0 2653 3498 Size: 2653 845 AleI CACnn'nnGTG Cuts at: 0 310 893 3498 Size: 310 583 2605 Fragments arranged by size: 2605 583 310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2258 3498 Size: 2258 1240 AlwNI CAG_nnn'CTG Cuts at: 0 912 2337 3205 3498 Size: 912 1425 868 293 Fragments arranged by size: 1425 912 868 293 ApaI G_GGCC'C Cuts at: 0 228 2285 3308 3498 Size: 228 2057 1023 190 Fragments arranged by size: 2057 1023 228 190 ApaLI G'TGCA_C Cuts at: 0 30 3498 Size: 30 3468 ApoI r'AATT_y Cuts at: 0 2847 3498 Size: 2847 651 AvaI C'yCGr_G Cuts at: 0 182 221 352 920 1054 1228 1273 1413 Size: 182 39 131 568 134 174 45 140 Cuts at: 1413 2097 2316 2376 2523 3498 Size: 684 219 60 147 975 Fragments arranged by size: 975 684 568 219 182 174 147 140 134 131 60 45 39 AvrII C'CTAG_G Cuts at: 0 2967 3498 Size: 2967 531 BanI G'GyrC_C Cuts at: 0 22 63 300 430 811 1112 1838 2131 Size: 22 41 237 130 381 301 726 293 Cuts at: 2131 2446 2464 2568 2752 3498 Size: 315 18 104 184 746 Fragments arranged by size: 746 726 381 315 301 293 237 184 130 104 41 22 18 BanII G_rGCy'C Cuts at: 0 228 416 637 2285 2813 2944 2957 3014 Size: 228 188 221 1648 528 131 13 57 Cuts at: 3014 3308 3498 Size: 294 190 Fragments arranged by size: 1648 528 294 228 221 190 188 131 57 13 BbeI G_GCGC'C Cuts at: 0 67 1116 1842 2135 2756 3498 Size: 67 1049 726 293 621 742 Fragments arranged by size: 1049 742 726 621 293 67 BbsI GAAGACnn'nnnn_ Cuts at: 0 248 404 964 1784 1822 3498 Size: 248 156 560 820 38 1676 Fragments arranged by size: 1676 820 560 248 156 38 BbvCI CC'TCA_GC Cuts at: 0 17 688 2490 3498 Size: 17 671 1802 1008 Fragments arranged by size: 1802 1008 671 17 BciVI GTATCCnnnnn_n' Cuts at: 0 675 3498 Size: 675 2823 BglI GCCn_nnn'nGGC Cuts at: 0 2275 2342 2548 2638 2983 3214 3498 Size: 2275 67 206 90 345 231 284 Fragments arranged by size: 2275 345 284 231 206 90 67 BglII A'GATC_T Cuts at: 0 2312 3498 Size: 2312 1186 BlpI GC'TnA_GC Cuts at: 0 112 3498 Size: 112 3386 Bme1580I G_kGCm'C Cuts at: 0 25 34 228 435 814 1211 1218 2285 Size: 25 9 194 207 379 397 7 1067 Cuts at: 2285 2449 3308 3332 3498 Size: 164 859 24 166 Fragments arranged by size: 1067 859 397 379 207 194 166 164 25 24 9 7 BmgBI CAC'GTC Cuts at: 0 1521 1694 1701 3498 Size: 1521 173 7 1797 Fragments arranged by size: 1797 1521 173 7 BmrI ACTGGGnnnn_n' Cuts at: 0 1205 2279 3498 Size: 1205 1074 1219 Fragments arranged by size: 1219 1205 1074 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 432 531 771 2613 3498 Size: 432 99 240 1842 885 Fragments arranged by size: 1842 885 432 240 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 457 874 1042 2190 2552 3319 3466 Size: 361 96 417 168 1148 362 767 147 Cuts at: 3466 3498 Size: 32 Fragments arranged by size: 1148 767 417 362 361 168 147 96 32 Bpu10I CC'TnA_GC Cuts at: 0 17 688 2191 2212 2490 3498 Size: 17 671 1503 21 278 1008 Fragments arranged by size: 1503 1008 671 278 21 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 721 3498 Size: 721 2777 BsaI GGTCTCn'nnnn_ Cuts at: 0 850 3223 3498 Size: 850 2373 275 Fragments arranged by size: 2373 850 275 BsaHI Gr'CG_yC Cuts at: 0 64 146 1113 1130 1839 2132 2753 3498 Size: 64 82 967 17 709 293 621 745 Fragments arranged by size: 967 745 709 621 293 82 64 17 BsaWI w'CCGG_w Cuts at: 0 3167 3261 3498 Size: 3167 94 237 Fragments arranged by size: 3167 237 94 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 136 166 520 550 619 649 1948 1978 Size: 136 30 354 30 69 30 1299 30 Cuts at: 1978 2077 2107 2283 2313 2758 2788 3222 3252 Size: 99 30 176 30 445 30 434 30 Cuts at: 3252 3498 Size: 246 Fragments arranged by size: 1299 445 434 354 246 176 136 99 69 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 307 424 454 504 648 733 799 1969 Size: 307 117 30 50 144 85 66 1170 Cuts at: 1969 2085 2167 3194 3498 Size: 116 82 1027 304 Fragments arranged by size: 1170 1027 307 304 144 117 116 85 82 66 50 30 BseYI C'CCAG_C Cuts at: 0 484 769 835 1177 1219 1736 2106 2127 Size: 484 285 66 342 42 517 370 21 Cuts at: 2127 2278 2452 2700 2812 3313 3396 3498 Size: 151 174 248 112 501 83 102 Fragments arranged by size: 517 501 484 370 342 285 248 174 151 112 102 83 66 42 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 405 610 1024 1833 3498 Size: 391 14 205 414 809 1665 Fragments arranged by size: 1665 809 414 391 205 14 BsiEI CG_ry'CG Cuts at: 0 3224 3498 Size: 3224 274 BsiHKAI G_wGCw'C Cuts at: 0 34 994 1466 1645 1693 2150 2431 2749 Size: 34 960 472 179 48 457 281 318 Cuts at: 2749 3498 Size: 749 Fragments arranged by size: 960 749 472 457 318 281 179 48 34 BsmI GAATG_Cn' Cuts at: 0 3115 3498 Size: 3115 383 BsmBI CGTCTCn'nnnn_ Cuts at: 0 243 1332 3498 Size: 243 1089 2166 Fragments arranged by size: 2166 1089 243 Bsp1286I G_dGCh'C Cuts at: 0 25 34 228 416 435 637 814 994 Size: 25 9 194 188 19 202 177 180 Cuts at: 994 1211 1218 1466 1645 1693 2150 2285 2431 Size: 217 7 248 179 48 457 135 146 Cuts at: 2431 2449 2749 2813 2944 2957 3014 3308 3332 Size: 18 300 64 131 13 57 294 24 Cuts at: 3332 3498 Size: 166 Fragments arranged by size: 457 300 294 248 217 202 194 188 180 179 177 166 146 135 131 64 57 48 25 24 19 18 13 9 7 BspEI T'CCGG_A Cuts at: 0 3167 3498 Size: 3167 331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1046 1653 2682 3023 3035 3422 3498 Size: 1046 607 1029 341 12 387 76 Fragments arranged by size: 1046 1029 607 387 341 76 12 BsrBI CCG'CTC Cuts at: 0 789 2093 3310 3498 Size: 789 1304 1217 188 Fragments arranged by size: 1304 1217 789 188 BsrFI r'CCGG_y Cuts at: 0 148 297 762 961 2017 2081 2749 3498 Size: 148 149 465 199 1056 64 668 749 Fragments arranged by size: 1056 749 668 465 199 149 148 64 BsrGI T'GTAC_A Cuts at: 0 1682 1979 3498 Size: 1682 297 1519 Fragments arranged by size: 1682 1519 297 BssHII G'CGCG_C Cuts at: 0 2102 3116 3498 Size: 2102 1014 382 Fragments arranged by size: 2102 1014 382 BssSI C'ACGA_G Cuts at: 0 1506 1686 1711 2149 3498 Size: 1506 180 25 438 1349 Fragments arranged by size: 1506 1349 438 180 25 BstAPI GCAn_nnn'nTGC Cuts at: 0 29 3498 Size: 29 3469 BstEII G'GTnAC_C Cuts at: 0 2694 3498 Size: 2694 804 BstXI CCAn_nnnn'nTGG Cuts at: 0 1429 1633 2460 3498 Size: 1429 204 827 1038 Fragments arranged by size: 1429 1038 827 204 BstYI r'GATC_y Cuts at: 0 3 2312 2594 3392 3498 Size: 3 2309 282 798 106 Fragments arranged by size: 2309 798 282 106 3 BtgI C'CryG_G Cuts at: 0 59 1316 1617 2421 2546 3242 3278 3284 Size: 59 1257 301 804 125 696 36 6 Cuts at: 3284 3498 Size: 214 Fragments arranged by size: 1257 804 696 301 214 125 59 36 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 158 404 1071 3498 Size: 158 246 667 2427 Fragments arranged by size: 2427 667 246 158 BtsI GCAGTG_nn' Cuts at: 0 204 263 900 1470 1653 1830 2004 2261 Size: 204 59 637 570 183 177 174 257 Cuts at: 2261 3093 3498 Size: 832 405 Fragments arranged by size: 832 637 570 405 257 204 183 177 174 59 DraIII CAC_nnn'GTG Cuts at: 0 29 3099 3498 Size: 29 3070 399 Fragments arranged by size: 3070 399 29 DrdI GACnn_nn'nnGTC Cuts at: 0 1606 3498 Size: 1606 1892 EaeI y'GGCC_r Cuts at: 0 599 760 1431 2418 2630 2829 3060 3221 Size: 599 161 671 987 212 199 231 161 Cuts at: 3221 3281 3498 Size: 60 217 Fragments arranged by size: 987 671 599 231 217 212 199 161 161 60 EagI C'GGCC_G Cuts at: 0 3221 3498 Size: 3221 277 EarI CTCTTCn'nnn_ Cuts at: 0 1051 1633 3127 3498 Size: 1051 582 1494 371 Fragments arranged by size: 1494 1051 582 371 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 336 1157 1516 2175 2941 3498 Size: 336 821 359 659 766 557 Fragments arranged by size: 821 766 659 557 359 336 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 106 361 457 485 874 976 1042 1303 Size: 106 255 96 28 389 102 66 261 Cuts at: 1303 1394 2190 2552 3108 3319 3466 3498 Size: 91 796 362 556 211 147 32 Fragments arranged by size: 796 556 389 362 261 255 211 147 106 102 96 91 66 32 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 2971 3079 3498 Size: 2971 108 419 Fragments arranged by size: 2971 419 108 EcoO109I rG'GnC_Cy Cuts at: 0 225 336 1153 2334 2610 2761 3304 3498 Size: 225 111 817 1181 276 151 543 194 Fragments arranged by size: 1181 817 543 276 225 194 151 111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 630 954 1308 1947 3105 3498 Size: 630 324 354 639 1158 393 Fragments arranged by size: 1158 639 630 393 354 324 FseI GG_CCGG'CC Cuts at: 0 766 3498 Size: 766 2732 FspI TGC'GCA Cuts at: 0 3031 3498 Size: 3031 467 HaeII r_GCGC'y Cuts at: 0 67 1116 1226 1764 1842 2082 2135 2219 Size: 67 1049 110 538 78 240 53 84 Cuts at: 2219 2756 2989 3498 Size: 537 233 509 Fragments arranged by size: 1049 538 537 509 240 233 110 84 78 67 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 136 168 2043 2075 3222 3254 3498 Size: 136 32 1875 32 1147 32 244 Fragments arranged by size: 1875 1147 244 136 32 32 32 HincII GTy'rAC Cuts at: 0 1289 2178 3498 Size: 1289 889 1320 Fragments arranged by size: 1320 1289 889 KasI G'GCGC_C Cuts at: 0 63 1112 1838 2131 2752 3498 Size: 63 1049 726 293 621 746 Fragments arranged by size: 1049 746 726 621 293 63 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2396 3498 Size: 2396 1102 MscI TGG'CCA Cuts at: 0 601 1433 2632 2831 3498 Size: 601 832 1199 199 667 Fragments arranged by size: 1199 832 667 601 199 MslI CAynn'nnrTG Cuts at: 0 310 893 1631 1663 3498 Size: 310 583 738 32 1835 Fragments arranged by size: 1835 738 583 310 32 MspA1I CmG'CkG Cuts at: 0 16 479 1593 2647 2947 3363 3498 Size: 16 463 1114 1054 300 416 135 Fragments arranged by size: 1114 1054 463 416 300 135 16 NaeI GCC'GGC Cuts at: 0 150 299 764 2019 2083 3498 Size: 150 149 465 1255 64 1415 Fragments arranged by size: 1415 1255 465 150 149 64 NarI GG'CG_CC Cuts at: 0 64 1113 1839 2132 2753 3498 Size: 64 1049 726 293 621 745 Fragments arranged by size: 1049 745 726 621 293 64 NcoI C'CATG_G Cuts at: 0 59 3242 3498 Size: 59 3183 256 Fragments arranged by size: 3183 256 59 NgoMIV G'CCGG_C Cuts at: 0 148 297 762 2017 2081 3498 Size: 148 149 465 1255 64 1417 Fragments arranged by size: 1417 1255 465 149 148 64 NspI r_CATG'y Cuts at: 0 564 1542 1662 3498 Size: 564 978 120 1836 Fragments arranged by size: 1836 978 564 120 PasI CC'CwG_GG Cuts at: 0 378 2331 3498 Size: 378 1953 1167 Fragments arranged by size: 1953 1167 378 PflMI CCAn_nnn'nTGG Cuts at: 0 126 3099 3498 Size: 126 2973 399 Fragments arranged by size: 2973 399 126 PfoI T'CCnGG_A Cuts at: 0 3488 3498 Size: 3488 10 PshAI GACnn'nnGTC Cuts at: 0 2786 3498 Size: 2786 712 PspOMI G'GGCC_C Cuts at: 0 224 2281 3304 3498 Size: 224 2057 1023 194 Fragments arranged by size: 2057 1023 224 194 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 268 300 3498 Size: 268 32 3198 Fragments arranged by size: 3198 268 32 PstI C_TGCA'G Cuts at: 0 48 1568 1606 1837 2646 3498 Size: 48 1520 38 231 809 852 Fragments arranged by size: 1520 852 809 231 48 38 PvuII CAG'CTG Cuts at: 0 16 2647 3363 3498 Size: 16 2631 716 135 Fragments arranged by size: 2631 716 135 16 SapI GCTCTTCn'nnn_ Cuts at: 0 1633 3127 3498 Size: 1633 1494 371 Fragments arranged by size: 1633 1494 371 SbfI CC_TGCA'GG Cuts at: 0 48 3498 Size: 48 3450 SexAI A'CCwGG_T Cuts at: 0 911 3498 Size: 911 2587 SfcI C'TryA_G Cuts at: 0 44 1564 1602 1833 2642 3498 Size: 44 1520 38 231 809 856 Fragments arranged by size: 1520 856 809 231 44 38 SfoI GGC'GCC Cuts at: 0 65 1114 1840 2133 2754 3498 Size: 65 1049 726 293 621 744 Fragments arranged by size: 1049 744 726 621 293 65 SgrAI Cr'CCGG_yG Cuts at: 0 2749 3498 Size: 2749 749 SmaI CCC'GGG Cuts at: 0 184 223 922 1056 1230 3498 Size: 184 39 699 134 174 2268 Fragments arranged by size: 2268 699 184 174 134 39 SmlI C'TyrA_G Cuts at: 0 736 2653 3498 Size: 736 1917 845 Fragments arranged by size: 1917 845 736 SphI G_CATG'C Cuts at: 0 1662 3498 Size: 1662 1836 SrfI GCCC'GGGC Cuts at: 0 223 922 3498 Size: 223 699 2576 Fragments arranged by size: 2576 699 223 StuI AGG'CCT Cuts at: 0 511 3498 Size: 511 2987 StyI C'CwwG_G Cuts at: 0 59 419 467 860 1160 1922 2135 2967 Size: 59 360 48 393 300 762 213 832 Cuts at: 2967 3131 3242 3428 3498 Size: 164 111 186 70 Fragments arranged by size: 832 762 393 360 300 213 186 164 111 70 59 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 261 318 562 796 1660 2452 2523 3498 Size: 261 57 244 234 864 792 71 975 Fragments arranged by size: 975 864 792 261 244 234 71 57 TatI w'GTAC_w Cuts at: 0 284 722 1682 1979 3498 Size: 284 438 960 297 1519 Fragments arranged by size: 1519 960 438 297 284 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 619 651 2077 2109 2140 2172 2283 2315 Size: 619 32 1426 32 31 32 111 32 Cuts at: 2315 2758 2790 3498 Size: 443 32 708 Fragments arranged by size: 1426 708 619 443 111 32 32 32 32 32 31 XbaI T'CTAG_A Cuts at: 0 2790 3444 3498 Size: 2790 654 54 Fragments arranged by size: 2790 654 54 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 196 312 1017 1179 2338 3240 3498 Size: 196 116 705 162 1159 902 258 Fragments arranged by size: 1159 902 705 258 196 162 116 XmaI C'CCGG_G Cuts at: 0 182 221 920 1054 1228 3498 Size: 182 39 699 134 174 2270 Fragments arranged by size: 2270 699 182 174 134 39 ZraI GAC'GTC Cuts at: 0 1131 3498 Size: 1131 2367 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PshAI PspOMI PsrI PstI PvuII SapI SbfI SexAI SfcI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XbaI XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AflIII AgeI AhdI AloI AscI AseI AsiSI BaeI BamHI BcgI BclI BfrBI BmtI BsaAI BsaBI BsiWI BspHI BsrDI BstBI BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspXI PvuI RsrII SacI SacII SalI SanDI ScaI SfiI SgfI SnaBI SpeI SspI SwaI Tth111I XhoI XmnI