Restriction map for
pFN21AE0997
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI BspDI
BspLU11I BsrBI BsrDI BsrFI BsrI
BssHII BssSI BstAPI BstBI BstUI
BstXI BstYI Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoT22I FbaI FseI FspI HaeIII
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeI MamI MboI
MfeI MflI MluI MluNI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 113 GGTACC G^GTACC
AccI 88 GTATAC GT^[AC][GT]AC
AciI 418 CCGC CCGC(-3/-1)
AcsI 435 GAATTT [GA]^AATT[CT]
AflIII 359 ACGTGT A^C[GA][CT]GT
Aor51HI 29 AGCGCT AGC^GCT
ApoI 435 GAATTT [GA]^AATT[CT]
Asp718I 113 GGTACC G^GTACC
BmyI 459 GGGCAC G[AGT]GC[ACT]^C
Bsp1286I 459 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 50 TGTACA T^GTACA
BspHI 296 TCATGA T^CATGA
BspMI 479 ACCTGC ACCTGC(4/8)
BsrGI 50 TGTACA T^GTACA
Bst1107I 88 GTATAC GTA^TAC
BstEII 15 GGTTACC G^GT[ACGT]ACC
BstPI 15 GGTTACC G^GT[ACGT]ACC
BstZ17I 88 GTATAC GTA^TAC
Cac8I 394 GCCTGC GC[ACGT]^[ACGT]GC
DraII 77 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 247 GAAGAG CTCTTC(1/4)
Eco47III 29 AGCGCT AGC^GCT
EcoO109I 77 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 15 GGTTACC G^GT[ACGT]ACC
EcoRV 100 GATATC GAT^ATC
EcoT14I 208 CCTTGG C^C[AT][AT]GG
FauI 417 CCCGC CCCGC(4/6)
HaeII 29 AGCGCT [GA]GCGC^[CT]
HgaI 303 GACGC GACGC(5/10)
HphI 441 GGTGA GGTGA(8/7)
KpnI 113 GGTACC GGTAC^C
Ksp632I 247 GAAGAG CTCTTC(1/4)
MaeII 359 ACGT A^CGT
MseI 140 TTAA T^TAA
MspA1I 418 CCGCTG C[AC]G^C[GT]G
NlaIII 297 CATG CATG^
PflMI 268 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 296 TCATGA T^CATGA
SexAI 314 ACCAGGT A^CC[AT]GGT
SmlI 193 CTCAAG C^T[CT][GA]AG
SspBI 50 TGTACA T^GTACA
StyI 208 CCTTGG C^C[AT][AT]GG
TaqI 386 TCGA T^CGA
Tru9I 140 TTAA T^TAA
Tsp45I 311 GTGAC ^GT[GC]AC
TthHB8I 386 TCGA T^CGA
Van91I 268 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 123 TCCGGA T^CCGGA
509 TCCGGA
AfaI 51 GTAC GT^AC
114 GTAC
AluI 164 AGCT AG^CT
215 AGCT
AvaII 78 GGACC G^G[AT]CC
512 GGACC
BanI 113 GGTACC G^G[CT][GA]CC
406 GGTGCC
BbvI 165 GCTGC GCAGC(8/12)
420 GCTGC
BsaJI 208 CCTTGG C^C[ACGT][ACGT]GG
456 CCAGGG
BsaWI 123 TCCGGA [AT]^CCGG[AT]
509 TCCGGA
BseAI 123 TCCGGA T^CCGGA
509 TCCGGA
BsgI 166 CTGCAC GTGCAG(16/14)
396 CTGCAC
BsiYI 117 CCAGAATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
268 CCATCCTCTGG
BslI 117 CCAGAATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
268 CCATCCTCTGG
BsmFI 77 GGGAC GGGAC(10/14)
221 GGGAC
BspEI 123 TCCGGA T^CCGGA
509 TCCGGA
CfoI 30 GCGC GCG^C
348 GCGC
Cfr13I 78 GGACC G^G[ACGT]CC
512 GGACC
DdeI 8 CTCAG C^T[ACGT]AG
96 CTCAG
Fnu4HI 165 GCTGC GC^[ACGT]GC
420 GCTGC
HapII 124 CCGG C^CGG
510 CCGG
HhaI 30 GCGC GCG^C
348 GCGC
HinP1I 30 GCGC G^CGC
348 GCGC
HpaII 124 CCGG C^CGG
510 CCGG
ItaI 165 GCTGC GC^[ACGT]GC
420 GCTGC
MaeIII 16 GTTAC ^GT[ACGT]AC
311 GTGAC
MroI 123 TCCGGA T^CCGGA
509 TCCGGA
MslI 291 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
294 CATCATGATG
MspI 124 CCGG C^CGG
510 CCGG
MwoI 198 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
207 GCCTTGGGAGC
RsaI 51 GTAC GT^AC
114 GTAC
Sau96I 78 GGACC G^G[ACGT]CC
512 GGACC
TseI 165 GCTGC G^C[AT]GC
420 GCTGC
TspRI 497 CACTG CA[GC]TG[ACGT][ACGT]^
520 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
Asp700I BssKI BstF5I BstNI EcoRII
FokI HinfI MboII MnlI MvaI
NlaIV ScrFI SfaNI TfiI Tsp509I
XmnI