(Linear) (Six-base) MAPSORT of: KIEE0997.seq  Check: 2697  from: 1  to: 525



KIEE0997.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:44  ..

AccI GT'mk_AC

Cuts at:      0      89     525 
   Size:         89     436
 
Acc65I G'GTAC_C

Cuts at:      0     113     525 
   Size:        113     412
 
AfeI AGC'GCT

Cuts at:      0      31     525 
   Size:         31     494
 
AflIII A'CryG_T

Cuts at:      0     359     525 
   Size:        359     166
 
ApoI r'AATT_y

Cuts at:      0     435     525 
   Size:        435      90
 
BanI G'GyrC_C

Cuts at:      0     113     406     525 
   Size:        113     293     119

  Fragments arranged by size: 

                 293     119     113
 
Bme1580I G_kGCm'C

Cuts at:      0     463     525 
   Size:        463      62
 
BmgBI CAC'GTC

Cuts at:      0     360     525 
   Size:        360     165
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     525 
   Size:        178     347
 
BsaWI w'CCGG_w

Cuts at:      0     123     509     525 
   Size:        123     386      16

  Fragments arranged by size: 

                 386     123      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     381     525 
   Size:        151     230     144

  Fragments arranged by size: 

                 230     151     144
 
Bsp1286I G_dGCh'C

Cuts at:      0     463     525 
   Size:        463      62
 
BspEI T'CCGG_A

Cuts at:      0     123     509     525 
   Size:        123     386      16

  Fragments arranged by size: 

                 386     123      16
 
BspHI T'CATG_A

Cuts at:      0     296     525 
   Size:        296     229
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     488     525 
   Size:        488      37
 
BsrGI T'GTAC_A

Cuts at:      0      50     525 
   Size:         50     475
 
BstEII G'GTnAC_C

Cuts at:      0      15     525 
   Size:         15     510
 
BstZ17I GTA'TAC

Cuts at:      0      90     525 
   Size:         90     435
 
BtsI GCAGTG_nn'

Cuts at:      0     496     519     525 
   Size:        496      23       6

  Fragments arranged by size: 

                 496      23       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     242     525 
   Size:        242     283
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     525 
   Size:         78     447
 
EcoRV GAT'ATC

Cuts at:      0     102     525 
   Size:        102     423
 
HaeII r_GCGC'y

Cuts at:      0      33     525 
   Size:         33     492
 
KpnI G_GTAC'C

Cuts at:      0     117     525 
   Size:        117     408
 
MslI CAynn'nnrTG

Cuts at:      0     295     298     525 
   Size:        295       3     227

  Fragments arranged by size: 

                 295     227       3
 
MspA1I CmG'CkG

Cuts at:      0     420     525 
   Size:        420     105
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     274     525 
   Size:        274     251
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     525 
   Size:         78     447
 
PsiI TTA'TAA

Cuts at:      0     131     525 
   Size:        131     394
 
SanDI GG'GwC_CC

Cuts at:      0      78     525 
   Size:         78     447
 
SexAI A'CCwGG_T

Cuts at:      0     314     525 
   Size:        314     211
 
SmlI C'TyrA_G

Cuts at:      0     193     525 
   Size:        193     332
 
StyI C'CwwG_G

Cuts at:      0     208     525 
   Size:        208     317
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     394     525 
   Size:        394     131
 
TatI w'GTAC_w

Cuts at:      0      50     525 
   Size:         50     475
 
XmnI GAAnn'nnTTC

Cuts at:      0      31     251     382     525 
   Size:         31     220     131     143

  Fragments arranged by size: 

                 220     143     131      31
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI   AflIII     ApoI     BanI Bme1580I    BmgBI
    BpuEI    BsaWI     BsgI Bsp1286I    BspEI    BspHI    BspMI    BsrGI
   BstEII  BstZ17I     BtsI     EarI EcoO109I    EcoRV    HaeII     KpnI
     MslI   MspA1I    PflMI    PpuMI     PsiI    SanDI    SexAI     SmlI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI