(Circular) (Six-base) MAPSORT of: pFN21AB6536.seq Check: 3307 from: 1 to: 5875 pFN21AB6536 5875 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2695 3218 1937 Size: 758 523 4594 Fragments arranged by size: 4594 758 523 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 3938 5322 5590 1632 Size: 1394 912 1384 268 1917 Fragments arranged by size: 1917 1394 1384 912 268 AfeI AGC'GCT Cuts at: 3095 3095 Size: 5875 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 2878 4774 5751 1203 Size: 1675 1896 977 1327 Fragments arranged by size: 1896 1675 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 2306 2855 3618 5190 2306 Size: 549 763 1572 2991 Fragments arranged by size: 2991 1572 763 549 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2549 3184 3410 3562 5671 1493 Size: 1056 635 226 152 2109 1697 Fragments arranged by size: 2109 1697 1056 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2631 3196 4651 618 Size: 325 217 627 844 565 1455 1842 Fragments arranged by size: 1842 1455 844 627 565 325 217 BanII G_rGCy'C Cuts at: 1391 2956 3194 1391 Size: 1565 238 4072 Fragments arranged by size: 4072 1565 238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BbvCI CC'TCA_GC Cuts at: 2995 2995 Size: 5875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4066 4100 1903 Size: 34 50 34 2045 34 3678 Fragments arranged by size: 3678 2045 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3087 3767 4977 3087 Size: 680 1210 3985 Fragments arranged by size: 3985 1210 680 BclI T'GATC_A Cuts at: 1548 2617 1548 Size: 1069 4806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2711 5870 2711 Size: 3159 2716 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 3936 5092 1790 Size: 2146 1156 2573 Fragments arranged by size: 2573 2146 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2394 2555 3606 Size: 416 53 83 399 24 977 161 1051 Cuts at: 3606 4564 442 Size: 958 1753 Fragments arranged by size: 1753 1051 977 958 416 399 161 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4535 1239 Size: 3296 2579 Bpu10I CC'TnA_GC Cuts at: 2995 2995 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2776 4000 4865 5163 2776 Size: 1224 865 298 3488 Fragments arranged by size: 3488 1224 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2758 2788 1178 Size: 30 165 30 1355 30 4265 Fragments arranged by size: 4265 1355 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2779 1186 Size: 1593 4282 BseYI C'CCAG_C Cuts at: 2949 5078 5574 2949 Size: 2129 496 3250 Fragments arranged by size: 3250 2129 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3936 4021 5092 3936 Size: 85 1071 4719 Fragments arranged by size: 4719 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2956 3194 3936 4021 5092 1391 Size: 399 1166 238 742 85 1071 2174 Fragments arranged by size: 2174 1166 1071 742 399 238 85 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 2217 2297 96 Size: 2121 80 3674 Fragments arranged by size: 3674 2121 80 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2046 3935 4947 2046 Size: 1889 1012 2974 Fragments arranged by size: 2974 1889 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 2238 4679 2238 Size: 2441 3434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2917 2917 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2711 3205 3572 3957 3974 5426 5512 5524 Size: 739 494 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 739 494 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5323 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 3391 4185 4205 3391 Size: 794 20 5061 Fragments arranged by size: 5061 794 20 ClaI AT'CG_AT Cuts at: 1554 2338 1554 Size: 784 5091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2656 2691 529 Size: 35 2092 35 3713 Fragments arranged by size: 3713 2092 35 35 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2415 2919 Size: 54 1425 50 275 18 345 240 504 Cuts at: 2919 3322 4211 5840 8 Size: 403 889 1629 43 Fragments arranged by size: 1629 1425 889 504 403 345 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2668 2868 3805 1353 Size: 1315 200 937 3423 Fragments arranged by size: 3423 1315 937 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3026 3938 4535 5322 5590 1239 Size: 393 1394 912 597 787 268 1524 Fragments arranged by size: 1524 1394 912 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3005 3005 Size: 5875 EcoRI G'AATT_C Cuts at: 2549 3184 3562 2549 Size: 635 378 4862 Fragments arranged by size: 4862 635 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3127 3127 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 3097 5022 1678 Size: 1419 1925 2531 Fragments arranged by size: 2531 1925 1419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2389 2421 4516 4548 4590 Size: 32 135 32 817 32 2095 32 42 Cuts at: 4590 4622 1373 Size: 32 2626 Fragments arranged by size: 2626 2095 817 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 2817 2973 3474 2817 Size: 156 501 5218 Fragments arranged by size: 5218 501 156 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2691 4989 5173 1305 Size: 1386 2298 184 2007 Fragments arranged by size: 2298 2007 1386 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 2921 10 Size: 54 1425 343 345 744 2964 Fragments arranged by size: 2964 1425 744 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3833 4192 4351 518 Size: 648 2667 359 159 2042 Fragments arranged by size: 2667 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3291 3968 5116 5361 1606 Size: 1685 677 1148 245 2120 Fragments arranged by size: 2120 1685 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5323 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2882 3233 4778 1132 Size: 97 1653 351 1545 2229 Fragments arranged by size: 2229 1653 1545 351 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 2878 4774 2878 Size: 1896 3979 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2644 2644 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3005 3005 Size: 5875 PshAI GACnn'nnGTC Cuts at: 1613 2657 1613 Size: 1044 4831 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2365 2397 2365 Size: 32 5843 PstI C_TGCA'G Cuts at: 830 3227 830 Size: 2397 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2868 2868 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2856 3223 4358 5039 5230 826 Size: 219 1811 367 1135 681 191 1471 Fragments arranged by size: 1811 1471 1135 681 367 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2791 3979 4880 5142 820 Size: 197 926 848 1188 901 262 1553 Fragments arranged by size: 1553 1188 926 901 848 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 2593 3120 3799 5 Size: 47 2541 527 679 2081 Fragments arranged by size: 2541 2081 679 527 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 3314 513 Size: 552 2249 3074 Fragments arranged by size: 3074 2249 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2413 3150 3902 3919 4072 4257 Size: 1255 606 227 737 752 17 153 185 Cuts at: 4257 4702 325 Size: 445 1498 Fragments arranged by size: 1498 1255 752 737 606 445 227 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 2297 4121 Size: 274 80 33 51 1443 240 80 1824 Cuts at: 4121 5833 96 Size: 1712 138 Fragments arranged by size: 1824 1712 1443 274 240 138 80 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2642 2642 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI