Restriction map for
pFN21AE1191
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BclI BfrI
BglI BlnI BsaAI BsaBI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
Eco47III EcoO65I EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 1227 GGCGTC G[GA]^CG[CT]C
AgeI 941 ACCGGT A^CCGGT
Alw44I 1035 GTGCAC G^TGCAC
ApaI 1743 GGGCCC GGGCC^C
ApaLI 1035 GTGCAC G^TGCAC
BalI 887 TGGCCA TGG^CCA
BamHI 1541 GGATCC G^GATCC
BbsI 1099 GAAGAC GAAGAC(2/6)
BbvCI 1127 CCTCAGC CCTCAGC(-5/-2)
BglII 1763 AGATCT A^GATCT
BpuAI 1099 GAAGAC GAAGAC(2/6)
BsaHI 1227 GGCGTC G[GA]^CG[CT]C
BsgI 2252 CTGCAC GTGCAG(16/14)
BsiEI 1869 CGGCCG CG[GA][CT]^CG
BsrDI 678 CATTGC GCAATG(2/0)
DraIII 1042 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1615 GACTCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1472 CCATGG C^C[GA][CT]GG
EagI 1869 CGGCCG C^GGCCG
EarI 1573 GAAGAG CTCTTC(1/4)
EclXI 1869 CGGCCG C^GGCCG
Eco52I 1869 CGGCCG C^GGCCG
EcoNI 1605 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1815 GATATC GAT^ATC
Hin1I 1227 GGCGTC G[GA]^CG[CT]C
HincII 730 GTTGAC GT[CT]^[GA]AC
HindII 730 GTTGAC GT[CT]^[GA]AC
Ksp632I 1573 GAAGAG CTCTTC(1/4)
MluNI 887 TGGCCA TGG^CCA
MscI 887 TGGCCA TGG^CCA
NaeI 1389 GCCGGC GCC^GGC
NcoI 1472 CCATGG C^CATGG
NdeI 442 CATATG CA^TATG
NgoMIV 1389 GCCGGC G^CCGGC
NotI 1868 GCGGCCGC GC^GGCCGC
NspI 1499 GCATGT [GA]CATG^[CT]
PinAI 941 ACCGGT A^CCGGT
SapI 1573 GAAGAGC GCTCTTC(1/4)
StuI 2451 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccII 119 CGCG CG^CG
1538 CGCG
AfaI 2112 GTAC GT^AC
2293 GTAC
AhdI 951 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2099 GACGGCAAGTC
AocI 845 CCTCAGG CC^T[ACGT]AGG
1681 CCTGAGG
AspEI 951 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2099 GACGGCAAGTC
BcgI 1185 CGACGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1477 GCACAGTTTTCG
BciVI 817 GGATAC GTATCC(6/5)
1651 GTATCC
BpmI 2455 CTCCAG CTGGAG(16/14)
877 CTGGAG
BsaWI 941 ACCGGT [AT]^CCGG[AT]
1559 TCCGGT
BseRI 1125 CTCCTC GAGGAG(10/8)
1767 CTCCTC
BsmAI 184 GTCTC GTCTC(1/5)
325 GTCTC
BsrBI 1466 CCGCTC CCGCTC(-3/-3)
1872 CCGCTC
BsrFI 941 ACCGGT [GA]^CCGG[CT]
1389 GCCGGC
BstUI 119 CGCG CG^CG
1538 CGCG
BstXI 137 CCACCGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1292 CCAAGCTGATGG
Bsu36I 845 CCTCAGG CC^T[ACGT]AGG
1681 CCTGAGG
Cfr10I 941 ACCGGT [GA]^CCGG[CT]
1389 GCCGGC
EaeI 887 TGGCCA [CT]^GGCC[GA]
1869 CGGCCG
Eam1105I 951 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2099 GACGGCAAGTC
Eco81I 845 CCTCAGG CC^T[ACGT]AGG
1681 CCTGAGG
EcoRI 250 GAATTC G^AATTC
748 GAATTC
EcoT14I 348 CCTTGG C^C[AT][AT]GG
1472 CCATGG
HaeII 1906 AGCGCC [GA]GCGC^[CT]
2170 AGCGCC
MslI 865 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1260 CACAGCCATG
MvnI 119 CGCG CG^CG
1538 CGCG
PpuMI 2056 GGGTCCT [GA]G^G[AT]CC[CT]
2365 GGGACCC
RsaI 2112 GTAC GT^AC
2293 GTAC
SbfI 1545 CCTGCAGG CCTGCA^GG
2286 CCTGCAGG
SmaI 1363 CCCGGG CCC^GGG
2053 CCCGGG
SmlI 1149 CTCAAG C^T[CT][GA]AG
1233 CTCAAG
Sse8387I 1545 CCTGCAGG CCTGCA^GG
2286 CCTGCAGG
StyI 348 CCTTGG C^C[AT][AT]GG
1472 CCATGG
TaqI 39 TCGA T^CGA
1184 TCGA
TthHB8I 39 TCGA T^CGA
1184 TCGA
XcmI 683 CCACCGTTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1289 CCACCAAGCTGATGG
XmaCI 1363 CCCGGG C^CCGGG
2053 CCCGGG
XmaI 1363 CCCGGG C^CCGGG
2053 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI Bpu1102I BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII