(Circular) (Six-base) MAPSORT of: pFN21AE1191.seq Check: 4015 from: 1 to: 7177 pFN21AE1191 7177 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3807 3961 3807 Size: 154 7023 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4987 278 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4520 1937 Size: 2583 4594 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 5304 5677 1563 Size: 3741 373 3063 Fragments arranged by size: 3741 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3652 3985 5240 6624 6892 1632 Size: 2020 333 1255 1384 268 1917 Fragments arranged by size: 2020 1917 1384 1255 333 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6980 AflIII A'CryG_T Cuts at: 1203 6076 7053 1203 Size: 4873 977 1327 Fragments arranged by size: 4873 1327 977 AgeI A'CCGG_T Cuts at: 2946 6008 2946 Size: 3062 4115 AhdI GACnn_n'nnGTC Cuts at: 2961 4109 5906 2961 Size: 1148 1797 4232 Fragments arranged by size: 4232 1797 1148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7177 AlwNI CAG_nnn'CTG Cuts at: 2911 3094 3442 3799 4920 6492 2911 Size: 183 348 357 1121 1572 3596 Fragments arranged by size: 3596 1572 1121 357 348 183 ApaI G_GGCC'C Cuts at: 3752 3752 Size: 7177 ApaLI G'TGCA_C Cuts at: 3040 5234 6390 3040 Size: 2194 1156 3827 Fragments arranged by size: 3827 2194 1156 ApoI r'AATT_y Cuts at: 1493 2255 2386 2395 2546 2570 2581 2753 2783 Size: 762 131 9 151 24 11 172 30 Cuts at: 2783 4486 4712 4864 6973 1493 Size: 1703 226 152 2109 1697 Fragments arranged by size: 2109 1703 1697 762 226 172 152 151 131 30 24 11 9 AseI AT'TA_AT Cuts at: 160 1032 5731 160 Size: 872 4699 1606 Fragments arranged by size: 4699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 3026 3202 3368 3740 4058 4473 4502 Size: 73 1083 176 166 372 318 415 29 Cuts at: 4502 1870 Size: 4545 Fragments arranged by size: 4545 1083 415 372 318 176 166 73 29 BamHI G'GATC_C Cuts at: 3546 4507 4874 3546 Size: 961 367 5849 Fragments arranged by size: 5849 961 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2656 3197 4303 4498 5953 Size: 325 217 627 869 541 1106 195 1455 Cuts at: 5953 618 Size: 1842 Fragments arranged by size: 1842 1455 1106 869 627 541 325 217 195 BanII G_rGCy'C Cuts at: 1391 2100 2889 3155 3375 3585 3752 4496 1391 Size: 709 789 266 220 210 167 744 4072 Fragments arranged by size: 4072 789 744 709 266 220 210 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 928 Size: 971 1212 4994 Fragments arranged by size: 4994 1212 971 BbvCI CC'TCA_GC Cuts at: 3133 3133 Size: 7177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3179 3213 3471 3505 5368 5402 1903 Size: 34 1242 34 258 34 1863 34 3678 Fragments arranged by size: 3678 1863 1242 258 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2816 3667 5069 6279 2816 Size: 851 1402 1210 3714 Fragments arranged by size: 3714 1402 1210 851 BclI T'GATC_A Cuts at: 1548 1548 Size: 7177 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5788 136 Size: 107 122 71 631 605 4116 1525 Fragments arranged by size: 4116 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3768 7172 3768 Size: 3404 3773 BlpI GC'TnA_GC Cuts at: 3374 3693 4031 4594 3374 Size: 319 338 563 5957 Fragments arranged by size: 5957 563 338 319 Bme1580I G_kGCm'C Cuts at: 1790 3044 3752 5238 6394 1790 Size: 1254 708 1486 1156 2573 Fragments arranged by size: 2573 1486 1254 1156 708 BmgBI CAC'GTC Cuts at: 1318 3193 1318 Size: 1875 5302 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4908 5866 442 Size: 416 53 83 399 24 3491 958 1753 Fragments arranged by size: 3491 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2058 2058 Size: 7177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2903 4445 5837 1239 Size: 1664 1542 1392 2579 Fragments arranged by size: 2579 1664 1542 1392 Bpu10I CC'TnA_GC Cuts at: 3133 3662 4019 3133 Size: 529 357 6291 Fragments arranged by size: 6291 529 357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3139 3223 5302 6167 6465 3139 Size: 84 2079 865 298 3851 Fragments arranged by size: 3851 2079 865 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5840 882 Size: 617 4341 2219 Fragments arranged by size: 4341 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4968 493 Size: 711 3764 2702 Fragments arranged by size: 3764 2702 711 BsaBI GATnn'nnATC Cuts at: 7171 7171 Size: 7177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3233 4984 5366 6001 Size: 53 83 186 936 1700 1751 382 635 Cuts at: 6001 275 Size: 1451 Fragments arranged by size: 1751 1700 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2946 3564 5610 6008 6282 6429 6840 2946 Size: 618 2046 398 274 147 411 3283 Fragments arranged by size: 3283 2046 618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3274 3304 3423 3453 3457 Size: 30 165 30 1871 30 119 30 4 Cuts at: 3457 3487 1178 Size: 30 4868 Fragments arranged by size: 4868 1871 165 119 30 30 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3121 3763 1186 Size: 1935 642 4600 Fragments arranged by size: 4600 1935 642 BseYI C'CCAG_C Cuts at: 2262 2842 3678 3735 3989 4220 4396 4407 6380 Size: 580 836 57 254 231 176 11 1973 Cuts at: 6380 6876 2262 Size: 496 2563 Fragments arranged by size: 2563 1973 836 580 496 254 231 176 57 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4242 1163 Size: 573 2506 4098 Fragments arranged by size: 4098 2506 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4627 5388 6416 1540 Size: 275 186 1876 750 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 750 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2809 2889 3044 3467 3585 4215 5238 5323 Size: 709 80 155 423 118 630 1023 85 Cuts at: 5323 6394 2100 Size: 1071 2883 Fragments arranged by size: 2883 1071 1023 709 630 423 155 118 85 80 BsmI GAATG_Cn' Cuts at: 4688 4781 4688 Size: 93 7084 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2809 2889 3044 3155 3375 3467 Size: 399 310 709 80 155 111 220 92 Cuts at: 3467 3585 3752 4215 4496 5238 5323 6394 1391 Size: 118 167 463 281 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 709 463 399 310 281 220 167 155 118 111 92 85 80 BspEI T'CCGG_A Cuts at: 6840 6840 Size: 7177 BspHI T'CATG_A Cuts at: 5066 6796 5066 Size: 1730 5447 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2835 3807 3961 4285 4299 4532 844 Size: 1991 972 154 324 14 233 3489 Fragments arranged by size: 3489 1991 972 324 233 154 14 BsrBI CCG'CTC Cuts at: 1394 1823 3473 3879 5064 1394 Size: 429 1650 406 1185 3507 Fragments arranged by size: 3507 1650 1185 429 406 BsrDI GCAATG_nn' Cuts at: 65 1248 2682 5672 5846 65 Size: 1183 1434 2990 174 1396 Fragments arranged by size: 2990 1434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2946 3394 5821 6008 1700 Size: 110 1136 448 2427 187 2869 Fragments arranged by size: 2869 2427 1136 448 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7177 BssSI C'ACGA_G Cuts at: 5237 6249 5237 Size: 1012 6165 BstAPI GCAn_nnn'nTGC Cuts at: 794 2914 3442 4210 4291 794 Size: 2120 528 768 81 3680 Fragments arranged by size: 3680 2120 768 528 81 BstBI TT'CG_AA Cuts at: 5981 5981 Size: 7177 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7177 BstXI CCAn_nnnn'nTGG Cuts at: 2149 3304 2149 Size: 1155 6022 BstYI r'GATC_y Cuts at: 1972 2973 3546 3591 3768 4507 4874 5259 5276 Size: 1001 573 45 177 739 367 385 17 Cuts at: 5276 6728 6814 6826 7172 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1001 739 573 385 367 346 177 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2851 3687 2851 Size: 836 6341 BtgI C'CryG_G Cuts at: 513 1065 2004 3477 513 Size: 552 939 1473 4213 Fragments arranged by size: 4213 1473 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4227 487 Size: 683 56 3001 3437 Fragments arranged by size: 3437 3001 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2975 3729 4693 5487 5507 2307 Size: 668 754 964 794 20 3977 Fragments arranged by size: 3977 964 794 754 668 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7142 DraI TTT'AAA Cuts at: 4481 4481 Size: 7177 DraIII CAC_nnn'GTG Cuts at: 3052 3052 Size: 7177 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3626 6184 809 Size: 507 2310 2558 1802 Fragments arranged by size: 2558 2310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2892 3874 4624 Size: 54 1425 50 275 18 1062 982 750 Cuts at: 4624 5513 7142 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1062 982 889 750 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 4624 1537 Size: 275 2062 750 4090 Fragments arranged by size: 4090 2062 750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3573 5107 1353 Size: 2220 1534 3423 Fragments arranged by size: 3423 2220 1534 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5764 6138 6284 387 Size: 1064 138 4175 374 146 1280 Fragments arranged by size: 4175 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2098 2887 3583 2098 Size: 789 696 5692 Fragments arranged by size: 5692 789 696 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2903 3652 3985 4445 5240 5837 6624 Size: 393 1271 749 333 460 795 597 787 Cuts at: 6624 6892 1239 Size: 268 1524 Fragments arranged by size: 1524 1271 795 787 749 597 460 393 333 268 EcoNI CCTnn'n_nnAGG Cuts at: 3614 3614 Size: 7177 EcoO109I rG'GnC_Cy Cuts at: 3121 3749 4062 4371 3121 Size: 628 313 309 5927 Fragments arranged by size: 5927 628 313 309 EcoRI G'AATT_C Cuts at: 2255 2753 4486 4864 2255 Size: 498 1733 378 4568 Fragments arranged by size: 4568 1733 498 378 EcoRV GAT'ATC Cuts at: 3822 3822 Size: 7177 FspI TGC'GCA Cuts at: 5683 5683 Size: 7177 HaeII r_GCGC'y Cuts at: 1678 3915 4179 6324 1678 Size: 2237 264 2145 2531 Fragments arranged by size: 2531 2237 2145 264 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2947 2979 3098 Size: 32 135 32 881 32 462 32 119 Cuts at: 3098 3130 3137 3169 4095 4127 5818 5850 5892 Size: 32 7 32 926 32 1691 32 42 Cuts at: 5892 5924 1373 Size: 32 2626 Fragments arranged by size: 2626 1691 926 881 462 135 119 42 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2737 4521 4767 669 Size: 988 281 799 1784 246 3079 Fragments arranged by size: 3079 1784 988 799 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7177 HpaI GTT'AAC Cuts at: 4767 4767 Size: 7177 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 4776 4776 Size: 7177 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2253 2349 2576 3648 6291 6475 1305 Size: 825 123 96 227 1072 2643 184 2007 Fragments arranged by size: 2643 2007 1072 825 227 184 123 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2894 10 Size: 54 1425 343 1062 4293 Fragments arranged by size: 4293 1425 1062 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2874 3269 5135 5494 5653 518 Size: 648 1708 395 1866 359 159 2042 Fragments arranged by size: 2042 1866 1708 648 395 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 3538 3653 3719 3873 3920 4593 5270 Size: 660 1272 115 66 154 47 673 677 Cuts at: 5270 6418 6663 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1272 1148 677 673 660 245 154 115 66 47 NaeI GCC'GGC Cuts at: 3396 3396 Size: 7177 NcoI C'CATG_G Cuts at: 513 1065 2004 3477 513 Size: 552 939 1473 4213 Fragments arranged by size: 4213 1473 939 552 NdeI CA'TA_TG Cuts at: 387 2448 387 Size: 2061 5116 NgoMIV G'CCGG_C Cuts at: 3394 3394 Size: 7177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7177 NotI GC'GGCC_GC Cuts at: 3874 4624 3874 Size: 750 6427 NspI r_CATG'y Cuts at: 1132 1229 3508 4535 6080 1132 Size: 97 2279 1027 1545 2229 Fragments arranged by size: 2279 2229 1545 1027 97 PasI CC'CwG_GG Cuts at: 1348 3829 4375 1348 Size: 2481 546 4150 Fragments arranged by size: 4150 2481 546 PciI A'CATG_T Cuts at: 6076 6076 Size: 7177 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7177 PfoI T'CCnGG_A Cuts at: 3594 3615 3594 Size: 21 7156 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5246 5278 6759 6791 5246 Size: 32 1481 32 5632 Fragments arranged by size: 5632 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4062 4371 4062 Size: 309 6868 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7177 PsiI TTA'TAA Cuts at: 2430 4747 2430 Size: 2317 4860 PspOMI G'GGCC_C Cuts at: 3748 3748 Size: 7177 PstI C_TGCA'G Cuts at: 830 2985 3462 3555 3645 3724 4254 4296 4529 Size: 2155 477 93 90 79 530 42 233 Cuts at: 4529 830 Size: 3478 Fragments arranged by size: 3478 2155 530 477 233 93 90 79 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 2266 3653 3719 3920 2266 Size: 1387 66 201 5523 Fragments arranged by size: 5523 1387 201 66 SacI G_AGCT'C Cuts at: 2100 2889 3585 2100 Size: 789 696 5692 Fragments arranged by size: 5692 789 696 SalI G'TCGA_C Cuts at: 1936 4519 1936 Size: 2583 4594 SanDI GG'GwC_CC Cuts at: 4371 4371 Size: 7177 SapI GCTCTTCn'nnn_ Cuts at: 3573 3573 Size: 7177 SbfI CC_TGCA'GG Cuts at: 3555 4296 4529 3555 Size: 741 233 6203 Fragments arranged by size: 6203 741 233 ScaI AGT'ACT Cuts at: 5425 7137 5425 Size: 1712 5465 SfcI C'TryA_G Cuts at: 826 1045 2981 3458 3551 3641 3704 3720 4250 Size: 219 1936 477 93 90 63 16 530 Cuts at: 4250 4292 4525 5660 6341 6532 826 Size: 42 233 1135 681 191 1471 Fragments arranged by size: 1936 1471 1135 681 530 477 233 219 191 93 90 63 42 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7177 SmaI CCC'GGG Cuts at: 1872 3370 4060 4504 1872 Size: 1498 690 444 4545 Fragments arranged by size: 4545 1498 690 444 SmlI C'TyrA_G Cuts at: 820 1017 1943 3154 3238 5281 6182 6444 820 Size: 197 926 1211 84 2043 901 262 1553 Fragments arranged by size: 2043 1553 1211 926 901 262 197 84 SnaBI TAC'GTA Cuts at: 493 4968 493 Size: 4475 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7177 SphI G_CATG'C Cuts at: 1132 4535 1132 Size: 3403 3774 SspI AAT'ATT Cuts at: 5 52 5101 5 Size: 47 5049 2081 Fragments arranged by size: 5049 2081 47 StuI AGG'CCT Cuts at: 1517 4458 1517 Size: 2941 4236 StyI C'CwwG_G Cuts at: 513 1065 2004 2353 3477 4616 513 Size: 552 939 349 1124 1139 3074 Fragments arranged by size: 3074 1139 1124 939 552 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2276 3185 3692 5204 5221 5374 5559 Size: 1255 696 909 507 1512 17 153 185 Cuts at: 5559 6004 325 Size: 445 1498 Fragments arranged by size: 1512 1498 1255 909 696 507 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5423 7135 96 Size: 274 80 33 51 1443 3446 1712 138 Fragments arranged by size: 3446 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3274 3306 3423 3455 3457 3489 7137 7169 3274 Size: 32 117 32 2 32 3648 32 3282 Fragments arranged by size: 3648 3282 117 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7177 XbaI T'CTAG_A Cuts at: 4513 4513 Size: 7177 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2695 3301 2695 Size: 606 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7177 XmaI C'CCGG_G Cuts at: 1870 3368 4058 4502 1870 Size: 1498 690 444 4545 Fragments arranged by size: 4545 1498 690 444 XmnI GAAnn'nnTTC Cuts at: 5306 5306 Size: 7177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4985 276 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI