(Linear) (Six-base) MAPSORT of: KIEE1191.seq Check: 2010 from: 1 to: 2472 KIEE1191.orf With 182 enzymes: SgfI * July 3, 2012 16:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1802 1956 2472 Size: 1802 154 516 Fragments arranged by size: 1802 516 154 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1647 1980 2472 Size: 1647 333 492 Fragments arranged by size: 1647 492 333 AgeI A'CCGG_T Cuts at: 0 941 2472 Size: 941 1531 AhdI GACnn_n'nnGTC Cuts at: 0 956 2104 2472 Size: 956 1148 368 Fragments arranged by size: 1148 956 368 AlwNI CAG_nnn'CTG Cuts at: 0 906 1089 1437 1794 2472 Size: 906 183 348 357 678 Fragments arranged by size: 906 678 357 348 183 ApaI G_GGCC'C Cuts at: 0 1747 2472 Size: 1747 725 ApaLI G'TGCA_C Cuts at: 0 1035 2472 Size: 1035 1437 ApoI r'AATT_y Cuts at: 0 250 381 390 541 565 576 748 778 Size: 250 131 9 151 24 11 172 30 Cuts at: 778 2472 Size: 1694 Fragments arranged by size: 1694 250 172 151 131 30 24 11 9 AvaI C'yCGr_G Cuts at: 0 1021 1197 1363 1735 2053 2472 Size: 1021 176 166 372 318 419 Fragments arranged by size: 1021 419 372 318 176 166 BamHI G'GATC_C Cuts at: 0 1541 2472 Size: 1541 931 BanI G'GyrC_C Cuts at: 0 651 1192 2298 2472 Size: 651 541 1106 174 Fragments arranged by size: 1106 651 541 174 BanII G_rGCy'C Cuts at: 0 95 884 1150 1370 1580 1747 2472 Size: 95 789 266 220 210 167 725 Fragments arranged by size: 789 725 266 220 210 167 95 BbsI GAAGACnn'nnnn_ Cuts at: 0 1106 2472 Size: 1106 1366 BbvCI CC'TCA_GC Cuts at: 0 1128 2472 Size: 1128 1344 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1174 1208 1466 1500 2472 Size: 1174 34 258 34 972 Fragments arranged by size: 1174 972 258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 811 1662 2472 Size: 811 851 810 Fragments arranged by size: 851 811 810 BglII A'GATC_T Cuts at: 0 1763 2472 Size: 1763 709 BlpI GC'TnA_GC Cuts at: 0 1369 1688 2026 2472 Size: 1369 319 338 446 Fragments arranged by size: 1369 446 338 319 Bme1580I G_kGCm'C Cuts at: 0 1039 1747 2472 Size: 1039 708 725 Fragments arranged by size: 1039 725 708 BmgBI CAC'GTC Cuts at: 0 1188 2472 Size: 1188 1284 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 53 2472 Size: 53 2419 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 898 2440 2472 Size: 898 1542 32 Fragments arranged by size: 1542 898 32 Bpu10I CC'TnA_GC Cuts at: 0 1128 1657 2014 2472 Size: 1128 529 357 458 Fragments arranged by size: 1128 529 458 357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1134 1218 2472 Size: 1134 84 1254 Fragments arranged by size: 1254 1134 84 BsaHI Gr'CG_yC Cuts at: 0 1228 2472 Size: 1228 1244 BsaWI w'CCGG_w Cuts at: 0 941 1559 2472 Size: 941 618 913 Fragments arranged by size: 941 913 618 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1269 1299 1418 1448 1452 1482 2472 Size: 1269 30 119 30 4 30 990 Fragments arranged by size: 1269 990 119 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1116 1758 2472 Size: 1116 642 714 Fragments arranged by size: 1116 714 642 BseYI C'CCAG_C Cuts at: 0 257 837 1673 1730 1984 2215 2391 2402 Size: 257 580 836 57 254 231 176 11 Cuts at: 2402 2472 Size: 70 Fragments arranged by size: 836 580 257 254 231 176 70 57 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2237 2472 Size: 2237 235 BsiEI CG_ry'CG Cuts at: 0 1872 2472 Size: 1872 600 BsiHKAI G_wGCw'C Cuts at: 0 95 804 884 1039 1462 1580 2210 2472 Size: 95 709 80 155 423 118 630 262 Fragments arranged by size: 709 630 423 262 155 118 95 80 Bsp1286I G_dGCh'C Cuts at: 0 95 804 884 1039 1150 1370 1462 1580 Size: 95 709 80 155 111 220 92 118 Cuts at: 1580 1747 2210 2472 Size: 167 463 262 Fragments arranged by size: 709 463 262 220 167 155 118 111 95 92 80 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 830 1802 1956 2280 2294 2472 Size: 830 972 154 324 14 178 Fragments arranged by size: 972 830 324 178 154 14 BsrBI CCG'CTC Cuts at: 0 1468 1874 2472 Size: 1468 406 598 Fragments arranged by size: 1468 598 406 BsrDI GCAATG_nn' Cuts at: 0 677 2472 Size: 677 1795 BsrFI r'CCGG_y Cuts at: 0 941 1389 2472 Size: 941 448 1083 Fragments arranged by size: 1083 941 448 BstAPI GCAn_nnn'nTGC Cuts at: 0 909 1437 2205 2286 2472 Size: 909 528 768 81 186 Fragments arranged by size: 909 768 528 186 81 BstXI CCAn_nnnn'nTGG Cuts at: 0 144 1299 2472 Size: 144 1155 1173 Fragments arranged by size: 1173 1155 144 BstYI r'GATC_y Cuts at: 0 968 1541 1586 1763 2472 Size: 968 573 45 177 709 Fragments arranged by size: 968 709 573 177 45 Bsu36I CC'TnA_GG Cuts at: 0 846 1682 2472 Size: 846 836 790 Fragments arranged by size: 846 836 790 BtgI C'CryG_G Cuts at: 0 1472 2472 Size: 1472 1000 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2222 2472 Size: 2222 250 BtsI GCAGTG_nn' Cuts at: 0 302 970 1724 2472 Size: 302 668 754 748 Fragments arranged by size: 754 748 668 302 DraIII CAC_nnn'GTG Cuts at: 0 1047 2472 Size: 1047 1425 DrdI GACnn_nn'nnGTC Cuts at: 0 1621 2472 Size: 1621 851 EaeI y'GGCC_r Cuts at: 0 887 1869 2472 Size: 887 982 603 Fragments arranged by size: 982 887 603 EagI C'GGCC_G Cuts at: 0 1869 2472 Size: 1869 603 EarI CTCTTCn'nnn_ Cuts at: 0 1568 2472 Size: 1568 904 EcoICRI GAG'CTC Cuts at: 0 93 882 1578 2472 Size: 93 789 696 894 Fragments arranged by size: 894 789 696 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 898 1647 1980 2440 2472 Size: 898 749 333 460 32 Fragments arranged by size: 898 749 460 333 32 EcoNI CCTnn'n_nnAGG Cuts at: 0 1609 2472 Size: 1609 863 EcoO109I rG'GnC_Cy Cuts at: 0 1116 1744 2057 2366 2472 Size: 1116 628 313 309 106 Fragments arranged by size: 1116 628 313 309 106 EcoRI G'AATT_C Cuts at: 0 250 748 2472 Size: 250 498 1724 Fragments arranged by size: 1724 498 250 EcoRV GAT'ATC Cuts at: 0 1817 2472 Size: 1817 655 HaeII r_GCGC'y Cuts at: 0 1910 2174 2472 Size: 1910 264 298 Fragments arranged by size: 1910 298 264 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 448 480 942 974 1093 1125 1132 1164 Size: 448 32 462 32 119 32 7 32 Cuts at: 1164 2090 2122 2472 Size: 926 32 350 Fragments arranged by size: 926 462 448 350 119 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 0 732 2472 Size: 732 1740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 125 248 344 571 1643 2472 Size: 125 123 96 227 1072 829 Fragments arranged by size: 1072 829 227 125 123 96 MscI TGG'CCA Cuts at: 0 889 2472 Size: 889 1583 MslI CAynn'nnrTG Cuts at: 0 869 1264 2472 Size: 869 395 1208 Fragments arranged by size: 1208 869 395 MspA1I CmG'CkG Cuts at: 0 261 1533 1648 1714 1868 1915 2472 Size: 261 1272 115 66 154 47 557 Fragments arranged by size: 1272 557 261 154 115 66 47 NaeI GCC'GGC Cuts at: 0 1391 2472 Size: 1391 1081 NcoI C'CATG_G Cuts at: 0 1472 2472 Size: 1472 1000 NdeI CA'TA_TG Cuts at: 0 443 2472 Size: 443 2029 NgoMIV G'CCGG_C Cuts at: 0 1389 2472 Size: 1389 1083 NotI GC'GGCC_GC Cuts at: 0 1869 2472 Size: 1869 603 NspI r_CATG'y Cuts at: 0 1503 2472 Size: 1503 969 PasI CC'CwG_GG Cuts at: 0 1824 2370 2472 Size: 1824 546 102 Fragments arranged by size: 1824 546 102 PfoI T'CCnGG_A Cuts at: 0 1589 1610 2472 Size: 1589 21 862 Fragments arranged by size: 1589 862 21 PpuMI rG'GwC_Cy Cuts at: 0 2057 2366 2472 Size: 2057 309 106 Fragments arranged by size: 2057 309 106 PsiI TTA'TAA Cuts at: 0 425 2472 Size: 425 2047 PspOMI G'GGCC_C Cuts at: 0 1743 2472 Size: 1743 729 PstI C_TGCA'G Cuts at: 0 980 1457 1550 1640 1719 2249 2291 2472 Size: 980 477 93 90 79 530 42 181 Fragments arranged by size: 980 530 477 181 93 90 79 42 PvuII CAG'CTG Cuts at: 0 261 1648 1714 1915 2472 Size: 261 1387 66 201 557 Fragments arranged by size: 1387 557 261 201 66 SacI G_AGCT'C Cuts at: 0 95 884 1580 2472 Size: 95 789 696 892 Fragments arranged by size: 892 789 696 95 SanDI GG'GwC_CC Cuts at: 0 2366 2472 Size: 2366 106 SapI GCTCTTCn'nnn_ Cuts at: 0 1568 2472 Size: 1568 904 SbfI CC_TGCA'GG Cuts at: 0 1550 2291 2472 Size: 1550 741 181 Fragments arranged by size: 1550 741 181 SfcI C'TryA_G Cuts at: 0 976 1453 1546 1636 1699 1715 2245 2287 Size: 976 477 93 90 63 16 530 42 Cuts at: 2287 2472 Size: 185 Fragments arranged by size: 976 530 477 185 93 90 63 42 16 SmaI CCC'GGG Cuts at: 0 1365 2055 2472 Size: 1365 690 417 Fragments arranged by size: 1365 690 417 SmlI C'TyrA_G Cuts at: 0 1149 1233 2472 Size: 1149 84 1239 Fragments arranged by size: 1239 1149 84 StuI AGG'CCT Cuts at: 0 2453 2472 Size: 2453 19 StyI C'CwwG_G Cuts at: 0 348 1472 2472 Size: 348 1124 1000 Fragments arranged by size: 1124 1000 348 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 271 1180 1687 2472 Size: 271 909 507 785 Fragments arranged by size: 909 785 507 271 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1269 1301 1418 1450 1452 1484 2472 Size: 1269 32 117 32 2 32 988 Fragments arranged by size: 1269 988 117 32 32 32 2 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 690 1296 2472 Size: 690 606 1176 Fragments arranged by size: 1176 690 606 XmaI C'CCGG_G Cuts at: 0 1363 2053 2472 Size: 1363 690 419 Fragments arranged by size: 1363 690 419 Enzymes that do cut: AarI AcuI AgeI AhdI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NotI NspI PasI PfoI PpuMI PsiI PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI BclI BfrBI BglI BmrI BmtI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI EciI FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NheI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI