Restriction map for
pFN21AB8222
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI MaeI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AflIII 506 ACATGT A^C[GA][CT]GT
ApaI 190 GGGCCC GGGCC^C
AviII 902 TGCGCA TGC^GCA
BbeI 76 GGCGCC GGCGC^C
BcgI 5 CGACGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 884 CGACCG CG[GA][CT]^CG
BsmAI 848 GAGAC GTCTC(1/5)
BspLU11I 506 ACATGT A^CATGT
BsrBI 784 GAGCGG CCGCTC(-3/-3)
BsrI 282 ACTGG ACTGG(1/-1)
BstAPI 765 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 803 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 28 CCGCGG C^C[GA][CT]GG
EarI 150 GAAGAG CTCTTC(1/4)
FseI 265 GGCCGGCC GGCCGG^CC
FspI 902 TGCGCA TGC^GCA
HaeII 76 GGCGCC [GA]GCGC^[CT]
HgaI 6 GACGC GACGC(5/10)
KasI 76 GGCGCC G^GCGCC
Ksp632I 150 GAAGAG CTCTTC(1/4)
KspI 28 CCGCGG CCGC^GG
NarI 76 GGCGCC GG^CGCC
PflMI 717 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 28 CCGCGG CCGC^GG
SfoI 76 GGCGCC GGC^GCC
TaqI 917 TCGA T^CGA
TthHB8I 917 TCGA T^CGA
Van91I 717 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 6 GACGCC G[GA]^CG[CT]C
76 GGCGCC
AfaI 744 GTAC GT^AC
861 GTAC
Alw44I 176 GTGCAC G^TGCAC
502 GTGCAC
ApaLI 176 GTGCAC G^TGCAC
502 GTGCAC
AspHI 176 GTGCAC G[AT]GC[AT]^C
502 GTGCAC
BanI 32 GGCACC G^G[CT][GA]CC
76 GGCGCC
BsaHI 6 GACGCC G[GA]^CG[CT]C
76 GGCGCC
BseRI 822 CTCCTC GAGGAG(10/8)
832 GAGGAG
BsiHKAI 176 GTGCAC G[AT]GC[AT]^C
502 GTGCAC
BsmFI 713 GGGAC GGGAC(10/14)
125 GTCCC
BspMI 35 ACCTGC ACCTGC(4/8)
687 ACCTGC
BstYI 635 AGATCC [GA]^GATC[CT]
701 GGATCT
DdeI 647 CTCAG C^T[ACGT]AG
825 CTCAG
DraII 190 GGGCCCT [GA]G^G[ACGT]CC[CT]
674 AGGCCCC
EaeI 102 CGGCCA [CT]^GGCC[GA]
578 TGGCCG
EcoNI 303 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
351 CCTACCCAAGG
EcoO109I 190 GGGCCCT [GA]G^G[ACGT]CC[CT]
674 AGGCCCC
EcoT14I 356 CCAAGG C^C[AT][AT]GG
803 CCAAGG
Hin1I 6 GACGCC G[GA]^CG[CT]C
76 GGCGCC
HinfI 328 GAGTC G^A[ACGT]TC
645 GACTC
HphI 685 TCACC GGTGA(8/7)
958 TCACC
MboII 150 GAAGA GAAGA(8/7)
286 GAAGA
MflI 635 AGATCC [GA]^GATC[CT]
701 GGATCT
MslI 106 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
181 CATGCCTGTG
NlaIII 181 CATG CATG^
507 CATG
NspI 180 ACATGC [GA]CATG^[CT]
506 ACATGT
PleI 645 GACTC GAGTC(4/5)
328 GAGTC
PstI 625 CTGCAG CTGCA^G
689 CTGCAG
RsaI 744 GTAC GT^AC
861 GTAC
SfaNI 243 GATGC GCATC(5/9)
771 GATGC
SfcI 625 CTGCAG C^T[GA][CT]AG
689 CTGCAG
StyI 356 CCAAGG C^C[AT][AT]GG
803 CCAAGG
XhoII 635 AGATCC [GA]^GATC[CT]
701 GGATCT
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIV
Sau3AI Sau96I ScrFI SmaI TseI
TspRI XmaCI XmaI