Restriction map for
pFN21AB8222
(Circular) (Six-base) MAPSORT of: pFN21AB8222.seq Check: 6525 from: 1 to: 5680
pFN21AB8222 5680 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2049 2701 2049
Size: 652 5028
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2189 3743 5127 5395 1632
Size: 542 15 1554 1384 268 1917
Fragments arranged by size:
1917 1554 1384 542 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2511 4579 5556 1203
Size: 1308 2068 977 1327
Fragments arranged by size:
2068 1327 1308 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2422 2728 2779 3423 4995 2422
Size: 306 51 644 1572 3107
Fragments arranged by size:
3107 1572 644 306 51
ApaI G_GGCC'C
Cuts at: 2199 2199
Size: 5680
ApaLI G'TGCA_C
Cuts at: 2181 2507 3737 4893 2181
Size: 326 1230 1156 2968
Fragments arranged by size:
2968 1230 1156 326
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2529 2875 3005 1870
Size: 73 189 397 346 130 4545
Fragments arranged by size:
4545 397 346 189 130 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2081 3001 4456 618
Size: 325 217 627 250 44 920 1455 1842
Fragments arranged by size:
1842 1455 920 627 325 250 217 44
BanII G_rGCy'C
Cuts at: 1391 2199 2235 2486 2529 2999 1391
Size: 808 36 251 43 470 4072
Fragments arranged by size:
4072 808 470 251 43 36
BbeI G_GCGC'C
Cuts at: 2085 2085
Size: 5680
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1999 2033 3871 3905 1903
Size: 34 62 34 1838 34 3678
Fragments arranged by size:
3678 1838 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2185 2199 2244 2511 2876 3741 4897
Size: 362 33 14 45 267 365 865 1156
Cuts at: 4897 1790
Size: 2573
Fragments arranged by size:
2573 1156 865 365 362 267 45 33
14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 2430 2444 2543 2807 4340 1239
Size: 1005 186 14 99 264 1533 2579
Fragments arranged by size:
2579 1533 1005 264 186 99 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2082 3487 3869
Size: 53 83 186 936 479 70 1405 382
Cuts at: 3869 4504 275
Size: 635 1451
Fragments arranged by size:
1451 1405 936 635 479 382 186 83
70 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2361 2391 2810 2840 1178
Size: 30 165 30 958 30 419 30 4018
Fragments arranged by size:
4018 958 419 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2818 2852 1186
Size: 1632 34 4014
Fragments arranged by size:
4014 1632 34
BseYI C'CCAG_C
Cuts at: 2277 2428 2957 4883 5379 2277
Size: 151 529 1926 496 2578
Fragments arranged by size:
2578 1926 529 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2610 2644 2955 1163
Size: 573 874 34 311 3888
Fragments arranged by size:
3888 874 573 311 34
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2892 3130 3891 4919 1540
Size: 275 186 891 238 761 1028 2301
Fragments arranged by size:
2301 1028 891 761 275 238 186
BsiHKAI G_wGCw'C
Cuts at: 2185 2511 3741 3826 4897 2185
Size: 326 1230 85 1071 2968
Fragments arranged by size:
2968 1230 1071 326 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2185 2199 2235 2244 2486 2511
Size: 399 362 33 14 36 9 242 25
Cuts at: 2511 2529 2876 2999 3741 3826 4897 1391
Size: 18 347 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 347 242 123
85 36 33 25 18 14 9
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2701 3035 844
Size: 1205 652 334 3489
Fragments arranged by size:
3489 1205 652 334
BsrBI CCG'CTC
Cuts at: 1394 1823 2791 3567 1394
Size: 429 968 776 3507
Fragments arranged by size:
3507 968 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 2253 2271 2585 2950 4324 4511
Size: 110 295 148 18 314 365 1374 187
Cuts at: 4511 1700
Size: 2869
Fragments arranged by size:
2869 1374 365 314 295 187 148 110
18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2776 794
Size: 1982 3698
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 2815
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2640 2706 3010 3377 3762 3779 5231 5317
Size: 668 66 304 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 668 385 367 346 304 86
66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2033 513
Size: 552 939 29 4160
Fragments arranged by size:
4160 939 552 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2670 2972 3196 3990 4010 2670
Size: 302 224 794 20 4340
Fragments arranged by size:
4340 794 302 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2583 3127
Size: 54 1425 50 275 18 277 476 544
Cuts at: 3127 4016 5645 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 544 476 277 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 3610 1353
Size: 797 1460 3423
Fragments arranged by size:
3423 1460 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2013 2340 2633 4267 4641 4787
Size: 1064 138 424 327 293 1634 374 146
Cuts at: 4787 387
Size: 1280
Fragments arranged by size:
1634 1280 1064 424 374 327 293 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2189 2244 2430 2444 2543 2807
Size: 393 542 15 55 186 14 99 264
Cuts at: 2807 3743 4340 5127 5395 1239
Size: 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 597 542 393 268 264
186 99 55 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2360 2312
Size: 48 5632
EcoO109I rG'GnC_Cy
Cuts at: 2196 2680 2196
Size: 484 5196
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FseI GG_CCGG'CC
Cuts at: 2275 2275
Size: 5680
FspI TGC'GCA
Cuts at: 2909 4186 2909
Size: 1277 4403
HaeII r_GCGC'y
Cuts at: 1678 2085 4827 1678
Size: 407 2742 2531
Fragments arranged by size:
2742 2531 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2361 2393 2810 2842 4321
Size: 32 135 32 789 32 417 32 1479
Cuts at: 4321 4353 4395 4427 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1479 789 417 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KasI G'GCGC_C
Cuts at: 2081 2081
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2523 4794 4978 1305
Size: 1218 2271 184 2007
Fragments arranged by size:
2271 2007 1218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2190 3638 3997 4156 518
Size: 648 949 75 1448 359 159 2042
Fragments arranged by size:
2042 1448 949 648 359 159 75
MspA1I CmG'CkG
Cuts at: 1606 2017 2035 2374 2725 3096 3773 4921 5166
Size: 411 18 339 351 371 677 1148 245
Cuts at: 5166 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 411 371 351 339 245
18
NaeI GCC'GGC
Cuts at: 2107 2255 2273 2952 2107
Size: 148 18 679 4835
Fragments arranged by size:
4835 679 148 18
NarI GG'CG_CC
Cuts at: 2082 2082
Size: 5680
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NgoMIV G'CCGG_C
Cuts at: 2105 2253 2271 2950 2105
Size: 148 18 679 4835
Fragments arranged by size:
4835 679 148 18
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2189 2515 3038 4583 1132
Size: 97 960 326 523 1545 2229
Fragments arranged by size:
2229 1545 960 523 326 97
PasI CC'CwG_GG
Cuts at: 1348 2395 2479 1348
Size: 1047 84 4549
Fragments arranged by size:
4549 1047 84
PciI A'CATG_T
Cuts at: 2511 4579 2511
Size: 2068 3612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2728 1108
Size: 1620 4060
PfoI T'CCnGG_A
Cuts at: 2214 2379 2446 2214
Size: 165 67 5448
Fragments arranged by size:
5448 165 67
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PspOMI G'GGCC_C
Cuts at: 2195 2195
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2634 2698 3032 830
Size: 1804 64 334 3478
Fragments arranged by size:
3478 1804 334 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SacII CC_GC'GG
Cuts at: 2036 2036
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2630 2694 3028 4163 4844 5035 826
Size: 219 1585 64 334 1135 681 191 1471
Fragments arranged by size:
1585 1471 1135 681 334 219 191 64
SfoI GGC'GCC
Cuts at: 2083 2083
Size: 5680
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2134 2531 2877 3007 1872
Size: 262 397 346 130 4545
Fragments arranged by size:
4545 397 346 262 130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SrfI GCCC'GGGC
Cuts at: 2877 2877
Size: 5680
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2808 3119 513
Size: 552 939 357 447 311 3074
Fragments arranged by size:
3074 939 552 447 357 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2382 2821 3707 3724 3877 4062 4507
Size: 1255 802 439 886 17 153 185 445
Cuts at: 4507 325
Size: 1498
Fragments arranged by size:
1498 1255 886 802 445 439 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 2132 2529 2875 3005 1870
Size: 262 397 346 130 4545
Fragments arranged by size:
4545 397 346 262 130
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SwaI XcmI