Restriction map for
pFN21AB8222
(Linear) (Six-base) MAPSORT of: KIBB8222.seq Check: 683 from: 1 to: 975
KIBB8222.orf
With 182 enzymes: SgfI *
July 3, 2012 16:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 44 696 975
Size: 44 652 279
Fragments arranged by size:
652 279 44
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 184 975
Size: 169 15 791
Fragments arranged by size:
791 169 15
AflIII A'CryG_T
Cuts at: 0 506 975
Size: 506 469
AlwNI CAG_nnn'CTG
Cuts at: 0 417 723 774 975
Size: 417 306 51 201
Fragments arranged by size:
417 306 201 51
ApaI G_GGCC'C
Cuts at: 0 194 975
Size: 194 781
ApaLI G'TGCA_C
Cuts at: 0 176 502 975
Size: 176 326 473
Fragments arranged by size:
473 326 176
AvaI C'yCGr_G
Cuts at: 0 127 524 870 975
Size: 127 397 346 105
Fragments arranged by size:
397 346 127 105
BanI G'GyrC_C
Cuts at: 0 32 76 975
Size: 32 44 899
Fragments arranged by size:
899 44 32
BanII G_rGCy'C
Cuts at: 0 194 230 481 524 975
Size: 194 36 251 43 451
Fragments arranged by size:
451 251 194 43 36
BbeI G_GCGC'C
Cuts at: 0 80 975
Size: 80 895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 975
Size: 28 947
Bme1580I G_kGCm'C
Cuts at: 0 147 180 194 239 506 871 975
Size: 147 33 14 45 267 365 104
Fragments arranged by size:
365 267 147 104 45 33 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 425 439 538 802 975
Size: 239 186 14 99 264 173
Fragments arranged by size:
264 239 186 173 99 14
BsaHI Gr'CG_yC
Cuts at: 0 7 77 975
Size: 7 70 898
Fragments arranged by size:
898 70 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 356 386 805 835 975
Size: 356 30 419 30 140
Fragments arranged by size:
419 356 140 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 813 847 975
Size: 813 34 128
Fragments arranged by size:
813 128 34
BseYI C'CCAG_C
Cuts at: 0 272 423 952 975
Size: 272 151 529 23
Fragments arranged by size:
529 272 151 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 639 950 975
Size: 605 34 311 25
Fragments arranged by size:
605 311 34 25
BsiEI CG_ry'CG
Cuts at: 0 887 975
Size: 887 88
BsiHKAI G_wGCw'C
Cuts at: 0 180 506 975
Size: 180 326 469
Fragments arranged by size:
469 326 180
Bsp1286I G_dGCh'C
Cuts at: 0 147 180 194 230 239 481 506 524
Size: 147 33 14 36 9 242 25 18
Cuts at: 524 871 975
Size: 347 104
Fragments arranged by size:
347 242 147 104 36 33 25 18
14 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 696 975
Size: 44 652 279
Fragments arranged by size:
652 279 44
BsrBI CCG'CTC
Cuts at: 0 786 975
Size: 786 189
BsrFI r'CCGG_y
Cuts at: 0 100 248 266 580 945 975
Size: 100 148 18 314 365 30
Fragments arranged by size:
365 314 148 100 30 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 771 975
Size: 771 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 975
Size: 810 165
BstYI r'GATC_y
Cuts at: 0 635 701 975
Size: 635 66 274
Fragments arranged by size:
635 274 66
BtgI C'CryG_G
Cuts at: 0 28 975
Size: 28 947
BtsI GCAGTG_nn'
Cuts at: 0 665 967 975
Size: 665 302 8
Fragments arranged by size:
665 302 8
EaeI y'GGCC_r
Cuts at: 0 102 578 975
Size: 102 476 397
Fragments arranged by size:
476 397 102
EarI CTCTTCn'nnn_
Cuts at: 0 145 975
Size: 145 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 8 335 628 975
Size: 8 327 293 347
Fragments arranged by size:
347 327 293 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 184 239 425 439 538 802 975
Size: 169 15 55 186 14 99 264 173
Fragments arranged by size:
264 186 173 169 99 55 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 355 975
Size: 307 48 620
Fragments arranged by size:
620 307 48
EcoO109I rG'GnC_Cy
Cuts at: 0 191 675 975
Size: 191 484 300
Fragments arranged by size:
484 300 191
FseI GG_CCGG'CC
Cuts at: 0 270 975
Size: 270 705
FspI TGC'GCA
Cuts at: 0 904 975
Size: 904 71
HaeII r_GCGC'y
Cuts at: 0 80 975
Size: 80 895
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 356 388 805 837 975
Size: 356 32 417 32 138
Fragments arranged by size:
417 356 138 32 32
KasI G'GCGC_C
Cuts at: 0 76 975
Size: 76 899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 975
Size: 518 457
MslI CAynn'nnrTG
Cuts at: 0 110 185 975
Size: 110 75 790
Fragments arranged by size:
790 110 75
MspA1I CmG'CkG
Cuts at: 0 12 30 369 720 975
Size: 12 18 339 351 255
Fragments arranged by size:
351 339 255 18 12
NaeI GCC'GGC
Cuts at: 0 102 250 268 947 975
Size: 102 148 18 679 28
Fragments arranged by size:
679 148 102 28 18
NarI GG'CG_CC
Cuts at: 0 77 975
Size: 77 898
NgoMIV G'CCGG_C
Cuts at: 0 100 248 266 945 975
Size: 100 148 18 679 30
Fragments arranged by size:
679 148 100 30 18
NspI r_CATG'y
Cuts at: 0 184 510 975
Size: 184 326 465
Fragments arranged by size:
465 326 184
PasI CC'CwG_GG
Cuts at: 0 390 474 975
Size: 390 84 501
Fragments arranged by size:
501 390 84
PciI A'CATG_T
Cuts at: 0 506 975
Size: 506 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 723 975
Size: 723 252
PfoI T'CCnGG_A
Cuts at: 0 209 374 441 975
Size: 209 165 67 534
Fragments arranged by size:
534 209 165 67
PspOMI G'GGCC_C
Cuts at: 0 190 975
Size: 190 785
PstI C_TGCA'G
Cuts at: 0 629 693 975
Size: 629 64 282
Fragments arranged by size:
629 282 64
SacII CC_GC'GG
Cuts at: 0 31 975
Size: 31 944
SfcI C'TryA_G
Cuts at: 0 625 689 975
Size: 625 64 286
Fragments arranged by size:
625 286 64
SfoI GGC'GCC
Cuts at: 0 78 975
Size: 78 897
SmaI CCC'GGG
Cuts at: 0 129 526 872 975
Size: 129 397 346 103
Fragments arranged by size:
397 346 129 103
SrfI GCCC'GGGC
Cuts at: 0 872 975
Size: 872 103
StyI C'CwwG_G
Cuts at: 0 356 803 975
Size: 356 447 172
Fragments arranged by size:
447 356 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 377 816 975
Size: 377 439 159
Fragments arranged by size:
439 377 159
XmaI C'CCGG_G
Cuts at: 0 127 524 870 975
Size: 127 397 346 105
Fragments arranged by size:
397 346 127 105
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BcgI Bme1580I BpmI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstXI BstYI BtgI BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PspOMI PstI SacII SfcI SfoI
SmaI SrfI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI