Restriction map for
pFN21AE2222
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MseI MslI
MspA1I MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tth111I
Van91I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 631 GAATTC [GA]^AATT[CT]
AcyI 465 GGCGCC G[GA]^CG[CT]C
AgeI 155 ACCGGT A^CCGGT
ApoI 631 GAATTC [GA]^AATT[CT]
AspHI 91 GAGCAC G[AT]GC[AT]^C
BalI 698 TGGCCA TGG^CCA
BbeI 465 GGCGCC GGCGC^C
BglII 333 AGATCT A^GATCT
BlpI 88 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 88 GCTGAGC GC^T[ACGT]AGC
BsaHI 465 GGCGCC G[GA]^CG[CT]C
BsaWI 155 ACCGGT [AT]^CCGG[AT]
BseRI 615 GAGGAG GAGGAG(10/8)
BsgI 271 GTGCAG GTGCAG(16/14)
BsiHKAI 91 GAGCAC G[AT]GC[AT]^C
BsmAI 618 GAGAC GTCTC(1/5)
BspHI 498 TCATGA T^CATGA
BsrFI 155 ACCGGT [GA]^CCGG[CT]
BstF5I 549 CATCC GGATG(2/0)
BstXI 51 CCACACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 88 GCTGAGC GC^T[ACGT]AGC
Cfr10I 155 ACCGGT [GA]^CCGG[CT]
DsaI 711 CCACGG C^C[GA][CT]GG
EaeI 698 TGGCCA [CT]^GGCC[GA]
EcoRI 631 GAATTC G^AATTC
FauI 4 GCGGG CCCGC(4/6)
FokI 549 CATCC GGATG(9/13)
HgaI 39 GCGTC GACGC(5/10)
Hin1I 465 GGCGCC G[GA]^CG[CT]C
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
HphI 186 GGTGA GGTGA(8/7)
KasI 465 GGCGCC G^GCGCC
MaeII 386 ACGT A^CGT
MluNI 698 TGGCCA TGG^CCA
MscI 698 TGGCCA TGG^CCA
NarI 465 GGCGCC GG^CGCC
PaeR7I 418 CTCGAG C^TCGAG
PinAI 155 ACCGGT A^CCGGT
RcaI 498 TCATGA T^CATGA
SfoI 465 GGCGCC GGC^GCC
SmlI 418 CTCGAG C^T[CT][GA]AG
Tsp509I 632 AATT ^AATT
XhoI 418 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 298 GATCC GGATC(4/5)
647 GGATC
AvaI 396 CCCGAG C^[CT]CG[GA]G
418 CTCGAG
AvaII 120 GGACC G^G[AT]CC
489 GGACC
BanI 197 GGCACC G^G[CT][GA]CC
465 GGCGCC
BcnI 214 CCCGG CC^[GC]GG
486 CCGGG
BpmI 181 CTGGAG CTGGAG(16/14)
255 CTGGAG
BsoBI 396 CCCGAG C^[CT]CG[GA]G
418 CTCGAG
BstYI 297 AGATCC [GA]^GATC[CT]
333 AGATCT
Cac8I 289 GCAGGC GC[ACGT]^[ACGT]GC
664 GCCAGC
MboII 336 TCTTC GAAGA(8/7)
339 TCTTC
MflI 297 AGATCC [GA]^GATC[CT]
333 AGATCT
NciI 214 CCCGG CC^[GC]GG
486 CCGGG
NlaIII 462 CATG CATG^
499 CATG
PleI 63 GAGTC GAGTC(4/5)
676 GAGTC
PpuMI 119 GGGACCC [GA]G^G[AT]CC[CT]
488 GGGACCT
Tsp45I 388 GTGAC ^GT[GC]AC
538 GTGAC
TspRI 54 CACTG CA[GC]TG[ACGT][ACGT]^
726 CACTG
XhoII 297 AGATCC [GA]^GATC[CT]
333 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MnlI MspI MvaI MwoI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TfiI TseI TthHB8I