(Circular) (Six-base) MAPSORT of: pFN21AE2222.seq Check: 3663 from: 1 to: 5443 pFN21AE2222 5443 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3253 278 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 1937 Size: 849 4594 Acc65I G'GTAC_C Cuts at: 2764 2764 Size: 5443 AclI AA'CG_TT Cuts at: 1563 3570 3943 1563 Size: 2007 373 3063 Fragments arranged by size: 3063 2007 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2685 3506 4890 5158 1632 Size: 1053 821 1384 268 1917 Fragments arranged by size: 1917 1384 1053 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5246 AflIII A'CryG_T Cuts at: 1203 4342 5319 1203 Size: 3139 977 1327 Fragments arranged by size: 3139 1327 977 AgeI A'CCGG_T Cuts at: 2160 4274 2160 Size: 2114 3329 AhdI GACnn_n'nnGTC Cuts at: 4172 4172 Size: 5443 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5443 AlwNI CAG_nnn'CTG Cuts at: 2093 2212 2252 3186 4758 2093 Size: 119 40 934 1572 2778 Fragments arranged by size: 2778 1572 934 119 40 ApaLI G'TGCA_C Cuts at: 3500 4656 3500 Size: 1156 4287 ApoI r'AATT_y Cuts at: 1493 2636 2752 2978 3130 5239 1493 Size: 1143 116 226 152 2109 1697 Fragments arranged by size: 2109 1697 1143 226 152 116 AseI AT'TA_AT Cuts at: 160 1032 3997 160 Size: 872 2965 1606 Fragments arranged by size: 2965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 AvaI C'yCGr_G Cuts at: 1870 1943 2401 2423 2768 1870 Size: 73 458 22 345 4545 Fragments arranged by size: 4545 458 345 73 22 BamHI G'GATC_C Cuts at: 2773 3140 2773 Size: 367 5076 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2202 2470 2764 4219 618 Size: 325 217 627 415 268 294 1455 1842 Fragments arranged by size: 1842 1455 627 415 325 294 268 217 BanII G_rGCy'C Cuts at: 1391 2762 1391 Size: 1371 4072 BbeI G_GCGC'C Cuts at: 2474 2474 Size: 5443 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3634 3668 1903 Size: 34 1697 34 3678 Fragments arranged by size: 3678 1697 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 4545 3335 Size: 1210 4233 BclI T'GATC_A Cuts at: 1548 1548 Size: 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4054 136 Size: 107 122 71 631 605 2382 1525 Fragments arranged by size: 2382 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2338 5438 2338 Size: 3100 2343 BlpI GC'TnA_GC Cuts at: 2094 2860 2094 Size: 766 4677 Bme1580I G_kGCm'C Cuts at: 1790 2384 2732 3504 4660 1790 Size: 594 348 772 1156 2573 Fragments arranged by size: 2573 1156 772 594 348 BmgBI CAC'GTC Cuts at: 1318 2392 1318 Size: 1074 4369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4132 442 Size: 416 53 83 399 24 1757 958 1753 Fragments arranged by size: 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2281 4103 1239 Size: 968 74 1822 2579 Fragments arranged by size: 2579 1822 968 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3568 4433 4731 3568 Size: 865 298 4280 Fragments arranged by size: 4280 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4106 882 Size: 617 2607 2219 Fragments arranged by size: 2607 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3234 493 Size: 711 2030 2702 Fragments arranged by size: 2702 2030 711 BsaBI GATnn'nnATC Cuts at: 5437 5437 Size: 5443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2471 3250 3632 4267 Size: 53 83 186 936 938 779 382 635 Cuts at: 4267 275 Size: 1451 Fragments arranged by size: 1451 938 936 779 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2160 3876 4274 4548 4695 5106 2160 Size: 1716 398 274 147 411 2497 Fragments arranged by size: 2497 1716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5218 Fragments arranged by size: 5218 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2635 1186 Size: 1449 3994 BseYI C'CCAG_C Cuts at: 2306 4646 5142 2306 Size: 2340 496 2607 Fragments arranged by size: 2607 2340 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 1163 Size: 573 561 4309 Fragments arranged by size: 4309 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2893 3654 4682 1540 Size: 275 186 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 3504 3589 4660 2100 Size: 1404 85 1071 2883 Fragments arranged by size: 2883 1404 1071 85 BsmI GAATG_Cn' Cuts at: 2954 3047 2954 Size: 93 5350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2384 2732 2762 3504 3589 4660 Size: 399 310 284 348 30 742 85 1071 Cuts at: 4660 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 348 310 284 85 30 BspEI T'CCGG_A Cuts at: 5106 5106 Size: 5443 BspHI T'CATG_A Cuts at: 2503 3332 5062 2503 Size: 829 1730 2884 Fragments arranged by size: 2884 1730 829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2731 2798 844 Size: 1887 67 3489 Fragments arranged by size: 3489 1887 67 BsrBI CCG'CTC Cuts at: 1394 1823 3330 1394 Size: 429 1507 3507 Fragments arranged by size: 3507 1507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3938 4112 65 Size: 1183 2690 174 1396 Fragments arranged by size: 2690 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 4087 4274 1700 Size: 110 350 1927 187 2869 Fragments arranged by size: 2869 1927 350 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5443 BssSI C'ACGA_G Cuts at: 3503 4515 3503 Size: 1012 4431 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5443 BstBI TT'CG_AA Cuts at: 4247 4247 Size: 5443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5443 BstXI CCAn_nnnn'nTGG Cuts at: 2063 2063 Size: 5443 BstYI r'GATC_y Cuts at: 1972 2302 2338 2773 3140 3525 3542 4994 5080 Size: 330 36 435 367 385 17 1452 86 Cuts at: 5080 5092 5438 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 435 385 367 346 330 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2716 513 Size: 552 939 712 3240 Fragments arranged by size: 3240 939 712 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4704 Fragments arranged by size: 4704 683 56 BtsI GCAGTG_nn' Cuts at: 2959 3753 3773 2959 Size: 794 20 4629 Fragments arranged by size: 4629 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5408 DraI TTT'AAA Cuts at: 2747 2747 Size: 5443 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4450 809 Size: 507 3134 1802 Fragments arranged by size: 3134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2703 2890 3779 Size: 54 1425 50 275 18 873 187 889 Cuts at: 3779 5408 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 873 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2890 1537 Size: 275 1078 4090 Fragments arranged by size: 4090 1078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3373 1353 Size: 2020 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4030 4404 4550 387 Size: 1064 138 2441 374 146 1280 Fragments arranged by size: 2441 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2281 2685 3506 4103 4890 5158 Size: 393 575 74 404 821 597 787 268 Cuts at: 5158 1239 Size: 1524 Fragments arranged by size: 1524 821 787 597 575 404 393 268 74 EcoO109I rG'GnC_Cy Cuts at: 2125 2256 2494 2125 Size: 131 238 5074 Fragments arranged by size: 5074 238 131 EcoRI G'AATT_C Cuts at: 2636 2752 3130 2636 Size: 116 378 4949 Fragments arranged by size: 4949 378 116 FspI TGC'GCA Cuts at: 3949 3949 Size: 5443 HaeII r_GCGC'y Cuts at: 1678 2094 2175 2474 4590 1678 Size: 416 81 299 2116 2531 Fragments arranged by size: 2531 2116 416 299 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 2294 2326 4084 Size: 32 135 32 529 32 161 32 1758 Cuts at: 4084 4116 4158 4190 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1758 529 161 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 2787 3033 669 Size: 988 281 349 500 246 3079 Fragments arranged by size: 3079 988 500 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5443 HpaI GTT'AAC Cuts at: 3033 3033 Size: 5443 KasI G'GCGC_C Cuts at: 2470 2470 Size: 5443 KpnI G_GTAC'C Cuts at: 2768 2768 Size: 5443 MfeI C'AATT_G Cuts at: 3042 3042 Size: 5443 MluI A'CGCG_T Cuts at: 5319 5319 Size: 5443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4557 4741 1305 Size: 3252 184 2007 Fragments arranged by size: 3252 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2705 10 Size: 54 1425 343 873 2748 Fragments arranged by size: 2748 1425 873 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3401 3760 3919 518 Size: 648 2235 359 159 2042 Fragments arranged by size: 2235 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2859 3536 4684 4929 1606 Size: 1253 677 1148 245 2120 Fragments arranged by size: 2120 1253 1148 677 245 NarI GG'CG_CC Cuts at: 2471 2471 Size: 5443 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3952 Fragments arranged by size: 3952 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5443 NotI GC'GGCC_GC Cuts at: 2890 2890 Size: 5443 NspI r_CATG'y Cuts at: 1132 1229 2801 4346 1132 Size: 97 1572 1545 2229 Fragments arranged by size: 2229 1572 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5443 PciI A'CATG_T Cuts at: 4342 4342 Size: 5443 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5443 PfoI T'CCnGG_A Cuts at: 2184 2490 2184 Size: 306 5137 PmeI GTTT'AAAC Cuts at: 2747 2747 Size: 5443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 5025 5057 3512 Size: 32 1481 32 3898 Fragments arranged by size: 3898 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2494 2125 Size: 369 5074 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5443 PsiI TTA'TAA Cuts at: 3013 3013 Size: 5443 PspXI vC'TCGA_Gb Cuts at: 2423 2423 Size: 5443 PstI C_TGCA'G Cuts at: 830 2795 830 Size: 1965 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5443 SalI G'TCGA_C Cuts at: 1936 2785 1936 Size: 849 4594 SanDI GG'GwC_CC Cuts at: 2125 2125 Size: 5443 SbfI CC_TGCA'GG Cuts at: 2795 2795 Size: 5443 ScaI AGT'ACT Cuts at: 3691 5403 3691 Size: 1712 3731 SfcI C'TryA_G Cuts at: 826 1045 2791 3926 4607 4798 826 Size: 219 1746 1135 681 191 1471 Fragments arranged by size: 1746 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2472 2472 Size: 5443 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5443 SmaI CCC'GGG Cuts at: 1872 2770 1872 Size: 898 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 3547 4448 4710 820 Size: 197 926 480 1124 901 262 1553 Fragments arranged by size: 1553 1124 926 901 480 262 197 SnaBI TAC'GTA Cuts at: 493 3234 493 Size: 2741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5443 SphI G_CATG'C Cuts at: 1132 2801 1132 Size: 1669 3774 SspI AAT'ATT Cuts at: 5 52 3367 5 Size: 47 3315 2081 Fragments arranged by size: 3315 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5443 StyI C'CwwG_G Cuts at: 513 1065 2004 2882 513 Size: 552 939 878 3074 Fragments arranged by size: 3074 939 878 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3470 3487 3640 3825 4270 325 Size: 1255 1890 17 153 185 445 1498 Fragments arranged by size: 1890 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 3689 5401 Size: 274 80 33 51 1443 717 995 1712 Cuts at: 5401 96 Size: 138 Fragments arranged by size: 1712 1443 995 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 5403 5435 2403 Size: 32 2968 32 2411 Fragments arranged by size: 2968 2411 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5443 XbaI T'CTAG_A Cuts at: 2779 2779 Size: 5443 XhoI C'TCGA_G Cuts at: 1943 2423 1943 Size: 480 4963 XmaI C'CCGG_G Cuts at: 1870 2768 1870 Size: 898 4545 XmnI GAAnn'nnTTC Cuts at: 3572 3572 Size: 5443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3251 276 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI