################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccI 420 GTATAC GT^[AC][GT]AC AcsI 1120 GAATTC [GA]^AATT[CT] AfaI 1440 GTAC GT^AC AflIII 686 ACATGT A^C[GA][CT]GT Alw44I 1378 GTGCAC G^TGCAC AlwNI 468 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1044 CCTCAGG CC^T[ACGT]AGG ApaLI 1378 GTGCAC G^TGCAC ApoI 1120 GAATTC [GA]^AATT[CT] AspI 586 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 937 CTCGAG C^[CT]CG[GA]G BbsI 607 GTCTTC GAAGAC(2/6) BbvCI 1431 CCTCAGC CCTCAGC(-5/-2) BciVI 944 GTATCC GTATCC(6/5) BclI 1397 TGATCA T^GATCA BfaI 1003 CTAG C^TAG BpuAI 607 GTCTTC GAAGAC(2/6) BsaWI 752 ACCGGA [AT]^CCGG[AT] BsiEI 630 CGACCG CG[GA][CT]^CG BsoBI 937 CTCGAG C^[CT]CG[GA]G BspLU11I 686 ACATGT A^CATGT Bst1107I 420 GTATAC GTA^TAC BstEII 1138 GGTGACC G^GT[ACGT]ACC BstPI 1138 GGTGACC G^GT[ACGT]ACC BstXI 1097 CCATGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1415 AGATCC [GA]^GATC[CT] BstZ17I 420 GTATAC GTA^TAC Bsu36I 1044 CCTCAGG CC^T[ACGT]AGG DraI 1210 TTTAAA TTT^AAA DraIII 1468 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1471 GACGTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1044 CCTCAGG CC^T[ACGT]AGG EcoO65I 1138 GGTGACC G^GT[ACGT]ACC EcoRI 1120 GAATTC G^AATTC FbaI 1397 TGATCA T^GATCA HaeII 552 GGCGCT [GA]GCGC^[CT] HincII 1342 GTCAAC GT[CT]^[GA]AC HindII 1342 GTCAAC GT[CT]^[GA]AC KspI 927 CCGCGG CCGC^GG MaeI 1003 CTAG C^TAG MflI 1415 AGATCC [GA]^GATC[CT] MseI 1211 TTAA T^TAA NaeI 523 GCCGGC GCC^GGC NcoI 1103 CCATGG C^CATGG NgoMIV 523 GCCGGC G^CCGGC PaeR7I 937 CTCGAG C^TCGAG PflFI 586 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 864 GGGTCCC [GA]G^G[AT]CC[CT] PstI 835 CTGCAG CTGCA^G RsaI 1440 GTAC GT^AC SacII 927 CCGCGG CCGC^GG SapI 888 GCTCTTC GCTCTTC(1/4) SexAI 78 ACCTGGT A^CC[AT]GGT SfiI 738 GGCCAGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SwaI 1209 ATTTAAAT ATTT^AAAT Tru9I 1211 TTAA T^TAA Tth111I 586 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1002 TCTAGA T^CTAGA XcmI 299 CCATCTTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 937 CTCGAG C^TCGAG XhoII 1415 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 1416 GATCC GGATC(4/5) 1240 GGATC ApaI 746 GGGCCC GGGCC^C 824 GGGCCC BalI 737 TGGCCA TGG^CCA 1094 TGGCCA BcgI 282 CGACTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 272 GCACCAGGCTCG BglI 730 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 739 GCCAGGCGGGC BsaI 1178 GAGACC GGTCTC(1/5) 487 GGTCTC BsmFI 674 GGGAC GGGAC(10/14) 866 GTCCC BsrFI 523 GCCGGC [GA]^CCGG[CT] 632 ACCGGC BssSI 162 CACGAG CACGAG(-5/-1) 478 CACGAG CfoI 13 GCGC GCG^C 553 GCGC Cfr10I 523 GCCGGC [GA]^CCGG[CT] 632 ACCGGC DraII 824 GGGCCCT [GA]G^G[ACGT]CC[CT] 864 GGGTCCC EcoO109I 824 GGGCCCT [GA]G^G[ACGT]CC[CT] 864 GGGTCCC EcoT14I 128 CCTTGG C^C[AT][AT]GG 1103 CCATGG HgaI 250 GACGC GACGC(5/10) 590 GCGTC HhaI 13 GCGC GCG^C 553 GCGC HinP1I 13 GCGC G^CGC 553 GCGC MaeII 1134 ACGT A^CGT 1472 ACGT MluNI 737 TGGCCA TGG^CCA 1094 TGGCCA MscI 737 TGGCCA TGG^CCA 1094 TGGCCA NspI 686 ACATGT [GA]CATG^[CT] 1292 ACATGC PleI 378 GACTC GAGTC(4/5) 534 GACTC SacI 394 GAGCTC GAGCT^C 934 GAGCTC SmlI 898 CTCAAG C^T[CT][GA]AG 937 CTCGAG StyI 128 CCTTGG C^C[AT][AT]GG 1103 CCATGG TfiI 903 GAATC G^A[AT]TC 1223 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AluI AspHI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I