Restriction map for
pFN21AE1945
(Circular) (Six-base) MAPSORT of: pFN21AE1945.seq Check: 6176 from: 1 to: 6205
pFN21AE1945 6205 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2426 3548 1937
Size: 489 1122 4594
Fragments arranged by size:
4594 1122 489
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3305 4268 5652 5920 1632
Size: 1302 371 963 1384 268 1917
Fragments arranged by size:
1917 1384 1302 963 371 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 2691 5104 6081 1203
Size: 1488 2413 977 1327
Fragments arranged by size:
2413 1488 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 3020 1166
Size: 1854 4351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3038 1184
Size: 1854 4351
AlwNI CAG_nnn'CTG
Cuts at: 2478 3948 5520 2478
Size: 1470 1572 3163
Fragments arranged by size:
3163 1572 1470
ApaI G_GGCC'C
Cuts at: 2755 2833 2755
Size: 78 6127
ApaLI G'TGCA_C
Cuts at: 3383 4262 5418 3383
Size: 879 1156 4170
Fragments arranged by size:
4170 1156 879
ApoI r'AATT_y
Cuts at: 1493 3125 3514 3740 3892 6001 1493
Size: 1632 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 1632 389 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2942 3530 1870
Size: 73 999 588 4545
Fragments arranged by size:
4545 999 588 73
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3526 4981 618
Size: 325 217 627 1739 1455 1842
Fragments arranged by size:
1842 1739 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 2755 2833 2943 3176 3524 1391
Size: 1012 352 78 110 233 348 4072
Fragments arranged by size:
4072 1012 352 348 233 110 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 928
Size: 971 706 4528
Fragments arranged by size:
4528 971 706
BbvCI CC'TCA_GC
Cuts at: 3437 3437
Size: 6205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2266 2276 2300 2310 4396 4430 1903
Size: 34 329 10 24 10 2086 34 3678
Fragments arranged by size:
3678 2086 329 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 2960 4097 5307 2960
Size: 1137 1210 3858
Fragments arranged by size:
3858 1210 1137
BclI T'GATC_A
Cuts at: 1548 3402 1548
Size: 1854 4351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2741 2750 4816
Size: 107 122 71 631 605 1069 9 2066
Cuts at: 4816 136
Size: 1525
Fragments arranged by size:
2066 1525 1069 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 2755 2833 3318 3387 3483 4266 5422 1790
Size: 965 78 485 69 96 783 1156 2573
Fragments arranged by size:
2573 1156 965 783 485 96 78 69
BmgBI CAC'GTC
Cuts at: 1318 3140 3478 1318
Size: 1822 338 4045
Fragments arranged by size:
4045 1822 338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 3497 3936
Size: 416 53 83 399 24 932 1148 439
Cuts at: 3936 4894 442
Size: 958 1753
Fragments arranged by size:
1753 1148 958 932 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2792 2813 4865 1239
Size: 1148 405 21 2052 2579
Fragments arranged by size:
2579 2052 1148 405 21
Bpu10I CC'TnA_GC
Cuts at: 3170 3437 3170
Size: 267 5938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2888 4330 5195 5493 2888
Size: 1442 865 298 3600
Fragments arranged by size:
3600 1442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2498 3177 4868 882
Size: 617 999 679 1691 2219
Fragments arranged by size:
2219 1691 999 679 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4012 4394 5029 275
Size: 53 83 186 936 2479 382 635 1451
Fragments arranged by size:
2479 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2757 4638 5036 5310 5457 5868 2757
Size: 1881 398 274 147 411 3094
Fragments arranged by size:
3094 1881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 1178
Size: 30 165 30 828 30 5122
Fragments arranged by size:
5122 828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2486 2810 2828 3143 3146 3349 1186
Size: 826 474 324 18 315 3 203 4042
Fragments arranged by size:
4042 826 474 324 315 203 18 3
BseYI C'CCAG_C
Cuts at: 2731 5408 5904 2731
Size: 2677 496 3032
Fragments arranged by size:
3032 2677 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2716 2771 1163
Size: 573 618 362 55 4597
Fragments arranged by size:
4597 618 573 362 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3655 4416 5444 1540
Size: 275 186 637 1017 761 1028 2301
Fragments arranged by size:
2301 1028 1017 761 637 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2943 3387 4266 4351 5422 2403
Size: 540 444 879 85 1071 3186
Fragments arranged by size:
3186 1071 879 540 444 85
BsmI GAATG_Cn'
Cuts at: 3716 3809 3716
Size: 93 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 2755 2833 2943 3176 3318 3387
Size: 399 613 352 78 110 233 142 69
Cuts at: 3387 3483 3524 4266 4351 5422 1391
Size: 96 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 352 233 142
110 96 85 78 69 41
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 2790 3141 3432 3560 844
Size: 1364 582 351 291 128 3489
Fragments arranged by size:
3489 1364 582 351 291 128
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4700 4874 65
Size: 1183 3452 174 1396
Fragments arranged by size:
3452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 2637 4849 5036 1700
Size: 110 718 109 2212 187 2869
Fragments arranged by size:
2869 2212 718 187 110 109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 2167 2483 4265 5277 2167
Size: 316 1782 1012 3095
Fragments arranged by size:
3095 1782 1012 316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 3143 1184
Size: 1959 4246
BstXI CCAn_nnnn'nTGG
Cuts at: 3109 3109
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 3420 3535 3902 4287 4304 5756 5842 5854
Size: 1448 115 367 385 17 1452 86 12
Cuts at: 5854 6200 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1448 385 367 346 115 86
17 12
BstZ17I GTA'TAC
Cuts at: 2427 2427
Size: 6205
Bsu36I CC'TnA_GG
Cuts at: 3050 3050
Size: 6205
BtgI C'CryG_G
Cuts at: 513 1065 2004 2659 2932 3108 513
Size: 552 939 655 273 176 3610
Fragments arranged by size:
3610 939 655 552 273 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2469 487
Size: 683 56 1243 4223
Fragments arranged by size:
4223 1243 683 56
BtsI GCAGTG_nn'
Cuts at: 2448 2479 2704 3322 3721 4515 4535 2448
Size: 31 225 618 399 794 20 4118
Fragments arranged by size:
4118 794 618 399 225 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3217 3509 3217
Size: 292 5913
DraIII CAC_nnn'GTG
Cuts at: 3478 3478
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3482 5212 809
Size: 507 2166 1730 1802
Fragments arranged by size:
2166 1802 1730 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2742 3099
Size: 54 1425 50 275 18 204 708 357
Cuts at: 3099 3652 4541 6170 8
Size: 553 889 1629 43
Fragments arranged by size:
1629 1425 889 708 553 357 275 204
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 2900 3164 4135 1353
Size: 1038 509 264 971 3423
Fragments arranged by size:
3423 1038 971 509 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3488 4792 5166 5312 387
Size: 1064 138 1899 1304 374 146 1280
Fragments arranged by size:
1899 1304 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2401 2941 2401
Size: 540 5665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2792 2813 2934 3305 4268 4865
Size: 393 755 405 21 121 371 963 597
Cuts at: 4865 5652 5920 1239
Size: 787 268 1524
Fragments arranged by size:
1524 963 787 755 597 405 393 371
268 121 21
EcoO109I rG'GnC_Cy
Cuts at: 2830 2870 2830
Size: 40 6165
EcoRI G'AATT_C
Cuts at: 3125 3514 3892 3125
Size: 389 378 5438
Fragments arranged by size:
5438 389 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2561 5352 1678
Size: 883 2791 2531
Fragments arranged by size:
2791 2531 883
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2974 3006 4846
Size: 32 135 32 659 32 711 32 1840
Cuts at: 4846 4878 4920 4952 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1840 711 659 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3349 3549 3795 669
Size: 988 281 1411 200 246 3079
Fragments arranged by size:
3079 1411 988 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5319 5503 1305
Size: 4014 184 2007
Fragments arranged by size:
4014 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2744 3101 10
Size: 54 1425 343 912 357 3114
Fragments arranged by size:
3114 1425 912 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2298 3020 3107 4163 4522 4681
Size: 648 1005 127 722 87 1056 359 159
Cuts at: 4681 518
Size: 2042
Fragments arranged by size:
2042 1056 1005 722 648 359 159 127
87
MspA1I CmG'CkG
Cuts at: 1606 2606 2845 2876 2934 3062 3621 4298 5446
Size: 1000 239 31 58 128 559 677 1148
Cuts at: 5446 5691 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1000 677 559 245 239 128
58 31
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 2004 3108 513
Size: 552 939 1104 3610
Fragments arranged by size:
3610 1104 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2695 3301 3563 5108 1132
Size: 97 1466 606 262 1545 2229
Fragments arranged by size:
2229 1545 1466 606 262 97
PasI CC'CwG_GG
Cuts at: 1348 3303 1348
Size: 1955 4250
PciI A'CATG_T
Cuts at: 2691 5104 2691
Size: 2413 3792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5303
PfoI T'CCnGG_A
Cuts at: 2235 2235
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2870 2870
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 2751 2829 2751
Size: 78 6127
PstI C_TGCA'G
Cuts at: 830 2844 3557 830
Size: 2014 713 3478
Fragments arranged by size:
3478 2014 713
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2606 2845 3062 2606
Size: 239 217 5749
Fragments arranged by size:
5749 239 217
SacI G_AGCT'C
Cuts at: 2403 2943 2403
Size: 540 5665
SacII CC_GC'GG
Cuts at: 2935 2935
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SanDI GG'GwC_CC
Cuts at: 2870 2870
Size: 6205
SapI GCTCTTCn'nnn_
Cuts at: 2900 2900
Size: 6205
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SexAI A'CCwGG_T
Cuts at: 2083 2083
Size: 6205
SfcI C'TryA_G
Cuts at: 826 1045 2460 2509 2840 3553 4688 5369 5560
Size: 219 1415 49 331 713 1135 681 191
Cuts at: 5560 826
Size: 1471
Fragments arranged by size:
1471 1415 1135 713 681 331 219 191
49
SfiI GGCCn_nnn'nGGCC
Cuts at: 2750 2750
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 2942 4309 5210 5472 820
Size: 197 926 960 39 1367 901 262 1553
Fragments arranged by size:
1553 1367 960 926 901 262 197 39
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 3108 3644 513
Size: 552 939 129 975 536 3074
Fragments arranged by size:
3074 975 939 552 536 129
SwaI ATTT'AAAT
Cuts at: 3217 3217
Size: 6205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 2792 3443 4232 4249 4402 4587
Size: 1255 1005 207 651 789 17 153 185
Cuts at: 4587 5032 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1005 789 651 445 207 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4451 6163 96
Size: 274 80 33 51 1443 2474 1712 138
Fragments arranged by size:
2474 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2678 2710 6165 6197 2678
Size: 32 3455 32 2686
Fragments arranged by size:
3455 2686 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2594 1316
Size: 1278 4927
XbaI T'CTAG_A
Cuts at: 3007 3541 3007
Size: 534 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2311
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 2942 1943
Size: 999 5206
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SfoI SrfI