(Linear) (Six-base) MAPSORT of: KIEE1945.seq Check: 5779 from: 1 to: 1500 KIEE1945.orf With 182 enzymes: SgfI * July 3, 2012 17:04 .. AccI GT'mk_AC Cuts at: 0 421 1500 Size: 421 1079 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 1300 1500 Size: 929 371 200 Fragments arranged by size: 929 371 200 AflIII A'CryG_T Cuts at: 0 686 1500 Size: 686 814 AleI CACnn'nnGTG Cuts at: 0 1015 1500 Size: 1015 485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1033 1500 Size: 1033 467 AlwNI CAG_nnn'CTG Cuts at: 0 473 1500 Size: 473 1027 ApaI G_GGCC'C Cuts at: 0 750 828 1500 Size: 750 78 672 Fragments arranged by size: 750 672 78 ApaLI G'TGCA_C Cuts at: 0 1378 1500 Size: 1378 122 ApoI r'AATT_y Cuts at: 0 1120 1500 Size: 1120 380 AvaI C'yCGr_G Cuts at: 0 937 1500 Size: 937 563 BanII G_rGCy'C Cuts at: 0 398 750 828 938 1171 1500 Size: 398 352 78 110 233 329 Fragments arranged by size: 398 352 329 233 110 78 BbsI GAAGACnn'nnnn_ Cuts at: 0 600 1500 Size: 600 900 BbvCI CC'TCA_GC Cuts at: 0 1432 1500 Size: 1432 68 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 261 271 295 305 1500 Size: 261 10 24 10 1195 Fragments arranged by size: 1195 261 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 0 955 1500 Size: 955 545 BclI T'GATC_A Cuts at: 0 1397 1500 Size: 1397 103 BglI GCCn_nnn'nGGC Cuts at: 0 736 745 1500 Size: 736 9 755 Fragments arranged by size: 755 736 9 Bme1580I G_kGCm'C Cuts at: 0 750 828 1313 1382 1478 1500 Size: 750 78 485 69 96 22 Fragments arranged by size: 750 485 96 78 69 22 BmgBI CAC'GTC Cuts at: 0 1135 1473 1500 Size: 1135 338 27 Fragments arranged by size: 1135 338 27 BmrI ACTGGGnnnn_n' Cuts at: 0 344 1500 Size: 344 1156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 787 808 1500 Size: 382 405 21 692 Fragments arranged by size: 692 405 382 21 Bpu10I CC'TnA_GC Cuts at: 0 1165 1432 1500 Size: 1165 267 68 Fragments arranged by size: 1165 267 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 883 1500 Size: 883 617 BsaI GGTCTCn'nnnn_ Cuts at: 0 493 1172 1500 Size: 493 679 328 Fragments arranged by size: 679 493 328 BsaWI w'CCGG_w Cuts at: 0 752 1500 Size: 752 748 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 226 256 1500 Size: 226 30 1244 Fragments arranged by size: 1244 226 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 481 805 823 1138 1141 1344 1500 Size: 7 474 324 18 315 3 203 156 Fragments arranged by size: 474 324 315 203 156 18 7 3 BseYI C'CCAG_C Cuts at: 0 726 1500 Size: 726 774 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 711 766 1500 Size: 349 362 55 734 Fragments arranged by size: 734 362 349 55 BsiEI CG_ry'CG Cuts at: 0 633 1500 Size: 633 867 BsiHKAI G_wGCw'C Cuts at: 0 398 938 1382 1500 Size: 398 540 444 118 Fragments arranged by size: 540 444 398 118 Bsp1286I G_dGCh'C Cuts at: 0 398 750 828 938 1171 1313 1382 1478 Size: 398 352 78 110 233 142 69 96 Cuts at: 1478 1500 Size: 22 Fragments arranged by size: 398 352 233 142 110 96 78 69 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 203 785 1136 1427 1500 Size: 203 582 351 291 73 Fragments arranged by size: 582 351 291 203 73 BsrFI r'CCGG_y Cuts at: 0 523 632 1500 Size: 523 109 868 Fragments arranged by size: 868 523 109 BssSI C'ACGA_G Cuts at: 0 162 478 1500 Size: 162 316 1022 Fragments arranged by size: 1022 316 162 BstEII G'GTnAC_C Cuts at: 0 1138 1500 Size: 1138 362 BstXI CCAn_nnnn'nTGG Cuts at: 0 1104 1500 Size: 1104 396 BstYI r'GATC_y Cuts at: 0 1415 1500 Size: 1415 85 BstZ17I GTA'TAC Cuts at: 0 422 1500 Size: 422 1078 Bsu36I CC'TnA_GG Cuts at: 0 1045 1500 Size: 1045 455 BtgI C'CryG_G Cuts at: 0 654 927 1103 1500 Size: 654 273 176 397 Fragments arranged by size: 654 397 273 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 464 1500 Size: 464 1036 BtsI GCAGTG_nn' Cuts at: 0 443 474 699 1317 1500 Size: 443 31 225 618 183 Fragments arranged by size: 618 443 225 183 31 DraI TTT'AAA Cuts at: 0 1212 1500 Size: 1212 288 DraIII CAC_nnn'GTG Cuts at: 0 1473 1500 Size: 1473 27 DrdI GACnn_nn'nnGTC Cuts at: 0 1477 1500 Size: 1477 23 EaeI y'GGCC_r Cuts at: 0 29 737 1094 1500 Size: 29 708 357 406 Fragments arranged by size: 708 406 357 29 EarI CTCTTCn'nnn_ Cuts at: 0 386 895 1159 1500 Size: 386 509 264 341 Fragments arranged by size: 509 386 341 264 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1483 1500 Size: 1483 17 EcoICRI GAG'CTC Cuts at: 0 396 936 1500 Size: 396 540 564 Fragments arranged by size: 564 540 396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 787 808 929 1300 1500 Size: 382 405 21 121 371 200 Fragments arranged by size: 405 382 371 200 121 21 EcoO109I rG'GnC_Cy Cuts at: 0 825 865 1500 Size: 825 40 635 Fragments arranged by size: 825 635 40 EcoRI G'AATT_C Cuts at: 0 1120 1500 Size: 1120 380 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1272 1500 Size: 1272 228 HaeII r_GCGC'y Cuts at: 0 556 1500 Size: 556 944 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 226 258 969 1001 1500 Size: 226 32 711 32 499 Fragments arranged by size: 711 499 226 32 32 HincII GTy'rAC Cuts at: 0 1344 1500 Size: 1344 156 MscI TGG'CCA Cuts at: 0 739 1096 1500 Size: 739 357 404 Fragments arranged by size: 739 404 357 MslI CAynn'nnrTG Cuts at: 0 166 293 1015 1102 1500 Size: 166 127 722 87 398 Fragments arranged by size: 722 398 166 127 87 MspA1I CmG'CkG Cuts at: 0 601 840 871 929 1057 1500 Size: 601 239 31 58 128 443 Fragments arranged by size: 601 443 239 128 58 31 NaeI GCC'GGC Cuts at: 0 525 1500 Size: 525 975 NcoI C'CATG_G Cuts at: 0 1103 1500 Size: 1103 397 NgoMIV G'CCGG_C Cuts at: 0 523 1500 Size: 523 977 NspI r_CATG'y Cuts at: 0 690 1296 1500 Size: 690 606 204 Fragments arranged by size: 690 606 204 PasI CC'CwG_GG Cuts at: 0 1298 1500 Size: 1298 202 PciI A'CATG_T Cuts at: 0 686 1500 Size: 686 814 PfoI T'CCnGG_A Cuts at: 0 230 1500 Size: 230 1270 PpuMI rG'GwC_Cy Cuts at: 0 865 1500 Size: 865 635 PspOMI G'GGCC_C Cuts at: 0 746 824 1500 Size: 746 78 676 Fragments arranged by size: 746 676 78 PstI C_TGCA'G Cuts at: 0 839 1500 Size: 839 661 PvuII CAG'CTG Cuts at: 0 601 840 1057 1500 Size: 601 239 217 443 Fragments arranged by size: 601 443 239 217 SacI G_AGCT'C Cuts at: 0 398 938 1500 Size: 398 540 562 Fragments arranged by size: 562 540 398 SacII CC_GC'GG Cuts at: 0 930 1500 Size: 930 570 SanDI GG'GwC_CC Cuts at: 0 865 1500 Size: 865 635 SapI GCTCTTCn'nnn_ Cuts at: 0 895 1500 Size: 895 605 SexAI A'CCwGG_T Cuts at: 0 78 1500 Size: 78 1422 SfcI C'TryA_G Cuts at: 0 455 504 835 1500 Size: 455 49 331 665 Fragments arranged by size: 665 455 331 49 SfiI GGCCn_nnn'nGGCC Cuts at: 0 745 1500 Size: 745 755 SmlI C'TyrA_G Cuts at: 0 898 937 1500 Size: 898 39 563 Fragments arranged by size: 898 563 39 StyI C'CwwG_G Cuts at: 0 128 1103 1500 Size: 128 975 397 Fragments arranged by size: 975 397 128 SwaI ATTT'AAAT Cuts at: 0 1212 1500 Size: 1212 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 580 787 1438 1500 Size: 580 207 651 62 Fragments arranged by size: 651 580 207 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 673 705 1500 Size: 673 32 795 Fragments arranged by size: 795 673 32 Tth111I GACn'n_nGTC Cuts at: 0 589 1500 Size: 589 911 XbaI T'CTAG_A Cuts at: 0 1002 1500 Size: 1002 498 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 1500 Size: 306 1194 XhoI C'TCGA_G Cuts at: 0 937 1500 Size: 937 563 Enzymes that do cut: AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BciVI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SfiI SmlI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BfrBI BglII BlpI BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI ClaI CspCI EagI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI TatI XmaI XmnI ZraI