################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BseRI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflIII 116 ACGTGT A^C[GA][CT]GT AhdI 1064 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 304 CCTGAGG CC^T[ACGT]AGG AspEI 1064 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1355 TGGCCA TGG^CCA BbsI 450 GTCTTC GAAGAC(2/6) BpuAI 450 GTCTTC GAAGAC(2/6) BsaAI 1780 TACGTG [CT]AC^GT[GA] BsiWI 1754 CGTACG C^GTACG BspHI 161 TCATGA T^CATGA BssHII 1588 GCGCGC G^CGCGC BssSI 494 CTCGTG CACGAG(-5/-1) BstEII 1302 GGTGACC G^GT[ACGT]ACC BstPI 1302 GGTGACC G^GT[ACGT]ACC BstXI 1082 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 304 CCTGAGG CC^T[ACGT]AGG DraIII 484 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1064 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 304 CCTGAGG CC^T[ACGT]AGG EcoO65I 1302 GGTGACC G^GT[ACGT]ACC EcoT14I 1186 CCATGG C^C[AT][AT]GG HgaI 1531 GCGTC GACGC(5/10) MluNI 1355 TGGCCA TGG^CCA MscI 1355 TGGCCA TGG^CCA MslI 1083 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1186 CCATGG C^CATGG NspI 942 GCATGC [GA]CATG^[CT] PshAI 372 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 161 TCATGA T^CATGA SacI 1327 GAGCTC GAGCT^C ScaI 476 AGTACT AGT^ACT SgrAI 1825 CGCCGGTG C[GA]^CCGG[CT]G SphI 942 GCATGC GCATG^C StyI 1186 CCATGG C^C[AT][AT]GG TaqI 312 TCGA T^CGA TthHB8I 312 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 893 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 1786 CAGAAACTG AspHI 66 GAGCAC G[AT]GC[AT]^C 1327 GAGCTC BfaI 505 CTAG C^TAG 1763 CTAG BlpI 81 GCTGAGC GC^T[ACGT]AGC 810 GCTCAGC Bpu1102I 81 GCTGAGC GC^T[ACGT]AGC 810 GCTCAGC BsiHKAI 66 GAGCAC G[AT]GC[AT]^C 1327 GAGCTC BsmAI 370 GAGAC GTCTC(1/5) 391 GTCTC BspMI 1181 ACCTGC ACCTGC(4/8) 651 GCAGGT CelII 81 GCTGAGC GC^T[ACGT]AGC 810 GCTCAGC EarI 13 GAAGAG CTCTTC(1/4) 1644 GAAGAG Ksp632I 13 GAAGAG CTCTTC(1/4) 1644 GAAGAG KspI 1396 CCGCGG CCGC^GG 1719 CCGCGG MaeI 505 CTAG C^TAG 1763 CTAG NaeI 1166 GCCGGC GCC^GGC 1422 GCCGGC NgoMIV 1166 GCCGGC G^CCGGC 1422 GCCGGC NotI 903 GCGGCCGC GC^GGCCGC 1398 GCGGCCGC PflMI 621 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 992 CCACCTACTGG PpuMI 836 GGGACCT [GA]G^G[AT]CC[CT] 1286 AGGACCC PstI 649 CTGCAG CTGCA^G 877 CTGCAG PvuII 374 CAGCTG CAG^CTG 1459 CAGCTG SacII 1396 CCGCGG CCGC^GG 1719 CCGCGG SapI 13 GAAGAGC GCTCTTC(1/4) 1644 GAAGAGC SexAI 323 ACCTGGT A^CC[AT]GGT 485 ACCAGGT SfaNI 1452 GATGC GCATC(5/9) 1581 GCATC SfcI 649 CTGCAG C^T[GA][CT]AG 877 CTGCAG SmlI 425 CTCAAG C^T[CT][GA]AG 472 CTCAAG Van91I 621 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 992 CCACCTACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfoI SmaI TseI Tsp45I TspRI XhoII XmaCI XmaI