(Circular) (Six-base) MAPSORT of: pFN21AB7848.seq Check: 2525 from: 1 to: 6571 pFN21AB7848 6571 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2647 2647 Size: 6571 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3914 1937 Size: 1977 4594 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3134 4634 6018 6286 1632 Size: 1502 1500 1384 268 1917 Fragments arranged by size: 1917 1502 1500 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 2121 5470 6447 1203 Size: 918 3349 977 1327 Fragments arranged by size: 3349 1327 977 918 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 3074 5300 3074 Size: 2226 4345 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3590 1184 Size: 2406 4165 AlwNI CAG_nnn'CTG Cuts at: 2903 3796 4314 5886 2903 Size: 893 518 1572 3588 Fragments arranged by size: 3588 1572 893 518 ApaI G_GGCC'C Cuts at: 2218 2703 3552 3620 2218 Size: 485 849 68 5169 Fragments arranged by size: 5169 849 485 68 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 3880 4106 4258 6367 1493 Size: 2387 226 152 2109 1697 Fragments arranged by size: 2387 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2043 3410 3510 3620 3896 1870 Size: 73 100 1367 100 110 276 4545 Fragments arranged by size: 4545 1367 276 110 100 100 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2425 2440 3168 3174 3374 Size: 325 217 627 638 15 728 6 200 Cuts at: 3374 3601 3629 3671 3729 3892 5347 618 Size: 227 28 42 58 163 1455 1842 Fragments arranged by size: 1842 1455 728 638 627 325 227 217 200 163 58 42 28 15 6 BanII G_rGCy'C Cuts at: 1391 2218 2703 3141 3336 3509 3552 3620 3890 Size: 827 485 438 195 173 43 68 270 Cuts at: 3890 1391 Size: 4072 Fragments arranged by size: 4072 827 485 438 270 195 173 68 43 BbeI G_GCGC'C Cuts at: 3178 3675 3733 3178 Size: 497 58 6016 Fragments arranged by size: 6016 497 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2448 928 Size: 971 549 5051 Fragments arranged by size: 5051 971 549 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4463 5673 4463 Size: 1210 5361 BclI T'GATC_A Cuts at: 1548 1548 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3159 3495 3513 Size: 107 122 71 631 605 1487 336 18 Cuts at: 3513 3763 5182 136 Size: 250 1419 1525 Fragments arranged by size: 1525 1487 1419 631 605 336 250 122 107 71 18 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 2087 2816 3988 2087 Size: 729 1172 4670 Fragments arranged by size: 4670 1172 729 Bme1580I G_kGCm'C Cuts at: 1790 2218 2445 2578 2703 3552 3604 3620 4632 Size: 428 227 133 125 849 52 16 1012 Cuts at: 4632 5788 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1012 849 428 227 133 125 52 16 BmgBI CAC'GTC Cuts at: 1318 2122 1318 Size: 804 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2439 4302 5260 Size: 416 53 83 399 24 1022 1863 958 Cuts at: 5260 442 Size: 1753 Fragments arranged by size: 1863 1753 1022 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3238 3238 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2528 2654 2765 5231 1239 Size: 863 426 126 111 2466 2579 Fragments arranged by size: 2579 2466 863 426 126 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2415 2462 4696 5561 5859 2415 Size: 47 2234 865 298 3127 Fragments arranged by size: 3127 2234 865 298 47 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5234 882 Size: 617 3735 2219 Fragments arranged by size: 3735 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3787 4362 493 Size: 711 2583 575 2702 Fragments arranged by size: 2702 2583 711 575 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3175 3672 3730 4378 Size: 53 83 186 936 1642 497 58 648 Cuts at: 4378 4760 5395 275 Size: 382 635 1451 Fragments arranged by size: 1642 1451 936 648 635 497 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 5004 5402 5676 5823 6234 5004 Size: 398 274 147 411 5341 Fragments arranged by size: 5341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 2922 2952 1178 Size: 30 165 30 825 30 664 30 4797 Fragments arranged by size: 4797 825 664 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6571 BseYI C'CCAG_C Cuts at: 3242 3710 5774 6270 3242 Size: 468 2064 496 3543 Fragments arranged by size: 3543 2064 496 468 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 3255 3372 3809 1163 Size: 573 891 628 117 437 3925 Fragments arranged by size: 3925 891 628 573 437 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2912 3407 3811 4021 4782 5810 Size: 275 186 911 495 404 210 761 1028 Cuts at: 5810 1540 Size: 2301 Fragments arranged by size: 2301 1028 911 761 495 404 275 210 186 BsiHKAI G_wGCw'C Cuts at: 2075 3336 4632 4717 5788 2075 Size: 1261 1296 85 1071 2858 Fragments arranged by size: 2858 1296 1261 1071 85 BsiWI C'GTAC_G Cuts at: 3759 3759 Size: 6571 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2218 2445 2578 2703 3141 3336 Size: 399 285 143 227 133 125 438 195 Cuts at: 3336 3509 3552 3604 3620 3890 4632 4717 5788 Size: 173 43 52 16 270 742 85 1071 Cuts at: 5788 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 438 399 285 270 227 195 173 143 133 125 85 52 43 16 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 2166 4460 6190 2166 Size: 2294 1730 2547 Fragments arranged by size: 2547 2294 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2647 3195 3926 844 Size: 1803 548 731 3489 Fragments arranged by size: 3489 1803 731 548 BsrBI CCG'CTC Cuts at: 1394 1823 2023 2914 3868 4458 1394 Size: 429 200 891 954 590 3507 Fragments arranged by size: 3507 954 891 590 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2646 3171 3377 3427 3626 3831 5215 Size: 110 836 525 206 50 199 205 1384 Cuts at: 5215 5402 1700 Size: 187 2869 Fragments arranged by size: 2869 1384 836 525 206 205 199 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6571 BssHII G'CGCG_C Cuts at: 1925 3593 1925 Size: 1668 4903 BssSI C'ACGA_G Cuts at: 2499 4631 5643 2499 Size: 2132 1012 3427 Fragments arranged by size: 3427 2132 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2650 2872 3022 3168 3796 794 Size: 1856 222 150 146 628 3569 Fragments arranged by size: 3569 1856 628 222 150 146 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 3307 1184 Size: 2123 4448 BstXI CCAn_nnnn'nTGG Cuts at: 3094 3094 Size: 6571 BstYI r'GATC_y Cuts at: 1972 2037 2889 2899 3901 4268 4653 4670 6122 Size: 65 852 10 1002 367 385 17 1452 Cuts at: 6122 6208 6220 6566 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1002 852 385 367 346 86 65 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2310 2310 Size: 6571 BtgI C'CryG_G Cuts at: 513 1065 2934 3191 3393 3401 3724 513 Size: 552 1869 257 202 8 323 3360 Fragments arranged by size: 3360 1869 552 323 257 202 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2106 487 Size: 683 56 880 4952 Fragments arranged by size: 4952 880 683 56 BtsI GCAGTG_nn' Cuts at: 2267 2731 4087 4881 4901 2267 Size: 464 1356 794 20 3937 Fragments arranged by size: 3937 1356 794 464 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3130 3165 3352 3387 529 Size: 35 2566 35 187 35 3713 Fragments arranged by size: 3713 2566 187 35 35 35 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DraIII CAC_nnn'GTG Cuts at: 2494 2494 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2909 3315 3360 Size: 54 1425 50 275 18 1079 406 45 Cuts at: 3360 3379 3404 3808 4018 4907 6536 8 Size: 19 25 404 210 889 1629 43 Fragments arranged by size: 1629 1425 1079 889 406 404 275 210 54 50 45 43 25 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2909 3404 3808 4018 1537 Size: 275 1097 495 404 210 4090 Fragments arranged by size: 4090 1097 495 404 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 3644 4501 1353 Size: 660 1631 857 3423 Fragments arranged by size: 3423 1631 857 660 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 3537 5158 5532 5678 387 Size: 1064 138 492 1456 1621 374 146 1280 Fragments arranged by size: 1621 1456 1280 1064 492 374 146 138 EcoICRI GAG'CTC Cuts at: 3334 3334 Size: 6571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2528 2654 2765 3134 4634 5231 Size: 393 470 426 126 111 369 1500 597 Cuts at: 5231 6018 6286 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1500 787 597 470 426 393 369 268 126 111 EcoO109I rG'GnC_Cy Cuts at: 2214 2215 2699 2700 2842 3292 3570 3616 3617 Size: 1 484 1 142 450 278 46 1 Cuts at: 3617 2214 Size: 5168 Fragments arranged by size: 5168 484 450 278 142 46 1 1 1 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 2108 3178 3480 3675 3721 3733 3842 5718 Size: 430 1070 302 195 46 12 109 1876 Cuts at: 5718 1678 Size: 2531 Fragments arranged by size: 2531 1876 1070 430 302 195 109 46 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2379 2411 3060 3092 5212 Size: 32 135 32 807 32 649 32 2120 Cuts at: 5212 5244 5286 5318 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2120 807 649 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3915 4161 669 Size: 988 281 1977 246 3079 Fragments arranged by size: 3079 1977 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KasI G'GCGC_C Cuts at: 3174 3671 3729 3174 Size: 497 58 6016 Fragments arranged by size: 6016 497 58 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5685 5869 1305 Size: 4380 184 2007 Fragments arranged by size: 4380 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3362 10 Size: 54 1425 343 1530 3219 Fragments arranged by size: 3219 1530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3092 4529 4888 5047 518 Size: 648 1926 1437 359 159 2042 Fragments arranged by size: 2042 1926 1437 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2381 2712 3265 3403 3466 3519 3726 3987 Size: 775 331 553 138 63 53 207 261 Cuts at: 3987 4664 5812 6057 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 677 553 331 261 245 207 138 63 53 NaeI GCC'GGC Cuts at: 3173 3429 3173 Size: 256 6315 NarI GG'CG_CC Cuts at: 3175 3672 3730 3175 Size: 497 58 6016 Fragments arranged by size: 6016 497 58 NcoI C'CATG_G Cuts at: 513 1065 3191 513 Size: 552 2126 3893 Fragments arranged by size: 3893 2126 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NgoMIV G'CCGG_C Cuts at: 3171 3427 3171 Size: 256 6315 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 2909 3404 4018 2909 Size: 495 614 5462 Fragments arranged by size: 5462 614 495 NspI r_CATG'y Cuts at: 1132 1229 2951 3929 5474 1132 Size: 97 1722 978 1545 2229 Fragments arranged by size: 2229 1722 1545 978 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 3003 1108 Size: 1524 371 4676 Fragments arranged by size: 4676 1524 371 PfoI T'CCnGG_A Cuts at: 2544 2742 2544 Size: 198 6373 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2842 3292 2842 Size: 450 6121 PshAI GACnn'nnGTC Cuts at: 1613 2381 1613 Size: 768 5803 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspOMI G'GGCC_C Cuts at: 2214 2699 3548 3616 2214 Size: 485 849 68 5169 Fragments arranged by size: 5169 849 485 68 PstI C_TGCA'G Cuts at: 830 2658 2886 3923 830 Size: 1828 228 1037 3478 Fragments arranged by size: 3478 1828 1037 228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2381 3466 2381 Size: 1085 5486 SacI G_AGCT'C Cuts at: 3336 3336 Size: 6571 SacII CC_GC'GG Cuts at: 3404 3727 3404 Size: 323 6248 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SapI GCTCTTCn'nnn_ Cuts at: 2013 3644 2013 Size: 1631 4940 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 2483 4819 6531 2483 Size: 2336 1712 2523 Fragments arranged by size: 2523 2336 1712 SexAI A'CCwGG_T Cuts at: 2328 2490 2328 Size: 162 6409 SfcI C'TryA_G Cuts at: 826 1045 2654 2882 3919 5054 5735 5926 826 Size: 219 1609 228 1037 1135 681 191 1471 Fragments arranged by size: 1609 1471 1135 1037 681 228 219 191 SfoI GGC'GCC Cuts at: 3176 3673 3731 3176 Size: 497 58 6016 Fragments arranged by size: 6016 497 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 3831 1700 Size: 2131 4440 SmaI CCC'GGG Cuts at: 1872 3412 3512 3622 3898 1872 Size: 1540 100 110 276 4545 Fragments arranged by size: 4545 1540 276 110 100 SmlI C'TyrA_G Cuts at: 820 1017 1943 2430 2477 4675 5576 5838 820 Size: 197 926 487 47 2198 901 262 1553 Fragments arranged by size: 2198 1553 926 901 487 262 197 47 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 2951 3929 1132 Size: 1819 978 3774 Fragments arranged by size: 3774 1819 978 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6571 StyI C'CwwG_G Cuts at: 513 1065 3191 4010 513 Size: 552 2126 819 3074 Fragments arranged by size: 3074 2126 819 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3406 4598 4615 4768 4953 5398 325 Size: 1255 1826 1192 17 153 185 445 1498 Fragments arranged by size: 1826 1498 1255 1192 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 3858 4817 Size: 274 80 33 51 1443 504 1377 959 Cuts at: 4817 6529 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1377 959 504 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 2922 2954 6531 6563 2228 Size: 32 662 32 3577 32 2236 Fragments arranged by size: 3577 2236 662 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6571 XmaI C'CCGG_G Cuts at: 1870 3410 3510 3620 3896 1870 Size: 1540 100 110 276 4545 Fragments arranged by size: 4545 1540 276 110 100 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI