(Linear) (Six-base) MAPSORT of: KIBB7848.seq Check: 7464 from: 1 to: 1866 KIBB7848.orf With 182 enzymes: SgfI * July 3, 2012 16:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 642 1866 Size: 642 1224 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1129 1866 Size: 1129 737 AflIII A'CryG_T Cuts at: 0 116 1866 Size: 116 1750 AhdI GACnn_n'nnGTC Cuts at: 0 1069 1866 Size: 1069 797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1585 1866 Size: 1585 281 AlwNI CAG_nnn'CTG Cuts at: 0 898 1791 1866 Size: 898 893 75 Fragments arranged by size: 898 893 75 ApaI G_GGCC'C Cuts at: 0 213 698 1547 1615 1866 Size: 213 485 849 68 251 Fragments arranged by size: 849 485 251 213 68 AvaI C'yCGr_G Cuts at: 0 38 1405 1505 1615 1866 Size: 38 1367 100 110 251 Fragments arranged by size: 1367 251 110 100 38 BanI G'GyrC_C Cuts at: 0 420 435 1163 1169 1369 1596 1624 1666 Size: 420 15 728 6 200 227 28 42 Cuts at: 1666 1724 1866 Size: 58 142 Fragments arranged by size: 728 420 227 200 142 58 42 28 15 6 BanII G_rGCy'C Cuts at: 0 213 698 1136 1331 1504 1547 1615 1866 Size: 213 485 438 195 173 43 68 251 Fragments arranged by size: 485 438 251 213 195 173 68 43 BbeI G_GCGC'C Cuts at: 0 1173 1670 1728 1866 Size: 1173 497 58 138 Fragments arranged by size: 1173 497 138 58 BbsI GAAGACnn'nnnn_ Cuts at: 0 443 1866 Size: 443 1423 BglI GCCn_nnn'nGGC Cuts at: 0 1154 1490 1508 1758 1866 Size: 1154 336 18 250 108 Fragments arranged by size: 1154 336 250 108 18 BlpI GC'TnA_GC Cuts at: 0 82 811 1866 Size: 82 729 1055 Fragments arranged by size: 1055 729 82 Bme1580I G_kGCm'C Cuts at: 0 213 440 573 698 1547 1599 1615 1866 Size: 213 227 133 125 849 52 16 251 Fragments arranged by size: 849 251 227 213 133 125 52 16 BmgBI CAC'GTC Cuts at: 0 117 1866 Size: 117 1749 BmrI ACTGGGnnnn_n' Cuts at: 0 434 1866 Size: 434 1432 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1233 1866 Size: 1233 633 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 523 649 760 1866 Size: 97 426 126 111 1106 Fragments arranged by size: 1106 426 126 111 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 457 1866 Size: 410 47 1409 Fragments arranged by size: 1409 410 47 BsaAI yAC'GTr Cuts at: 0 1782 1866 Size: 1782 84 BsaHI Gr'CG_yC Cuts at: 0 1170 1667 1725 1866 Size: 1170 497 58 141 Fragments arranged by size: 1170 497 141 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 223 253 917 947 1866 Size: 223 30 664 30 919 Fragments arranged by size: 919 664 223 30 30 BseYI C'CCAG_C Cuts at: 0 1237 1705 1866 Size: 1237 468 161 Fragments arranged by size: 1237 468 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 1250 1367 1804 1866 Size: 622 628 117 437 62 Fragments arranged by size: 628 622 437 117 62 BsiEI CG_ry'CG Cuts at: 0 907 1402 1806 1866 Size: 907 495 404 60 Fragments arranged by size: 907 495 404 60 BsiHKAI G_wGCw'C Cuts at: 0 70 1331 1866 Size: 70 1261 535 Fragments arranged by size: 1261 535 70 BsiWI C'GTAC_G Cuts at: 0 1754 1866 Size: 1754 112 Bsp1286I G_dGCh'C Cuts at: 0 70 213 440 573 698 1136 1331 1504 Size: 70 143 227 133 125 438 195 173 Cuts at: 1504 1547 1599 1615 1866 Size: 43 52 16 251 Fragments arranged by size: 438 251 227 195 173 143 133 125 70 52 43 16 BspHI T'CATG_A Cuts at: 0 161 1866 Size: 161 1705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 642 1190 1866 Size: 642 548 676 Fragments arranged by size: 676 642 548 BsrBI CCG'CTC Cuts at: 0 18 909 1863 1866 Size: 18 891 954 3 Fragments arranged by size: 954 891 18 3 BsrFI r'CCGG_y Cuts at: 0 641 1166 1372 1422 1621 1826 1866 Size: 641 525 206 50 199 205 40 Fragments arranged by size: 641 525 206 205 199 50 40 BssHII G'CGCG_C Cuts at: 0 1588 1866 Size: 1588 278 BssSI C'ACGA_G Cuts at: 0 494 1866 Size: 494 1372 BstAPI GCAn_nnn'nTGC Cuts at: 0 645 867 1017 1163 1791 1866 Size: 645 222 150 146 628 75 Fragments arranged by size: 645 628 222 150 146 75 BstEII G'GTnAC_C Cuts at: 0 1302 1866 Size: 1302 564 BstXI CCAn_nnnn'nTGG Cuts at: 0 1089 1866 Size: 1089 777 BstYI r'GATC_y Cuts at: 0 32 884 894 1866 Size: 32 852 10 972 Fragments arranged by size: 972 852 32 10 Bsu36I CC'TnA_GG Cuts at: 0 305 1866 Size: 305 1561 BtgI C'CryG_G Cuts at: 0 929 1186 1388 1396 1719 1866 Size: 929 257 202 8 323 147 Fragments arranged by size: 929 323 257 202 147 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 101 1866 Size: 101 1765 BtsI GCAGTG_nn' Cuts at: 0 262 726 1866 Size: 262 464 1140 Fragments arranged by size: 1140 464 262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1125 1160 1347 1382 1866 Size: 1125 35 187 35 484 Fragments arranged by size: 1125 484 187 35 35 DraIII CAC_nnn'GTG Cuts at: 0 489 1866 Size: 489 1377 EaeI y'GGCC_r Cuts at: 0 904 1310 1355 1374 1399 1803 1866 Size: 904 406 45 19 25 404 63 Fragments arranged by size: 904 406 404 63 45 25 19 EagI C'GGCC_G Cuts at: 0 904 1399 1803 1866 Size: 904 495 404 63 Fragments arranged by size: 904 495 404 63 EarI CTCTTCn'nnn_ Cuts at: 0 8 1639 1866 Size: 8 1631 227 Fragments arranged by size: 1631 227 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 76 1532 1866 Size: 76 1456 334 Fragments arranged by size: 1456 334 76 EcoICRI GAG'CTC Cuts at: 0 1329 1866 Size: 1329 537 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 523 649 760 1129 1866 Size: 97 426 126 111 369 737 Fragments arranged by size: 737 426 369 126 111 97 EcoO109I rG'GnC_Cy Cuts at: 0 209 210 694 695 837 1287 1565 1611 Size: 209 1 484 1 142 450 278 46 Cuts at: 1611 1612 1866 Size: 1 254 Fragments arranged by size: 484 450 278 254 209 142 46 1 1 1 HaeII r_GCGC'y Cuts at: 0 103 1173 1475 1670 1716 1728 1837 1866 Size: 103 1070 302 195 46 12 109 29 Fragments arranged by size: 1070 302 195 109 103 46 29 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 374 406 1055 1087 1866 Size: 374 32 649 32 779 Fragments arranged by size: 779 649 374 32 32 KasI G'GCGC_C Cuts at: 0 1169 1666 1724 1866 Size: 1169 497 58 142 Fragments arranged by size: 1169 497 142 58 MscI TGG'CCA Cuts at: 0 1357 1866 Size: 1357 509 MslI CAynn'nnrTG Cuts at: 0 1087 1866 Size: 1087 779 MspA1I CmG'CkG Cuts at: 0 376 707 1260 1398 1461 1514 1721 1866 Size: 376 331 553 138 63 53 207 145 Fragments arranged by size: 553 376 331 207 145 138 63 53 NaeI GCC'GGC Cuts at: 0 1168 1424 1866 Size: 1168 256 442 Fragments arranged by size: 1168 442 256 NarI GG'CG_CC Cuts at: 0 1170 1667 1725 1866 Size: 1170 497 58 141 Fragments arranged by size: 1170 497 141 58 NcoI C'CATG_G Cuts at: 0 1186 1866 Size: 1186 680 NgoMIV G'CCGG_C Cuts at: 0 1166 1422 1866 Size: 1166 256 444 Fragments arranged by size: 1166 444 256 NotI GC'GGCC_GC Cuts at: 0 904 1399 1866 Size: 904 495 467 Fragments arranged by size: 904 495 467 NspI r_CATG'y Cuts at: 0 946 1866 Size: 946 920 PflMI CCAn_nnn'nTGG Cuts at: 0 627 998 1866 Size: 627 371 868 Fragments arranged by size: 868 627 371 PfoI T'CCnGG_A Cuts at: 0 539 737 1866 Size: 539 198 1129 Fragments arranged by size: 1129 539 198 PpuMI rG'GwC_Cy Cuts at: 0 837 1287 1866 Size: 837 450 579 Fragments arranged by size: 837 579 450 PshAI GACnn'nnGTC Cuts at: 0 376 1866 Size: 376 1490 PspOMI G'GGCC_C Cuts at: 0 209 694 1543 1611 1866 Size: 209 485 849 68 255 Fragments arranged by size: 849 485 255 209 68 PstI C_TGCA'G Cuts at: 0 653 881 1866 Size: 653 228 985 Fragments arranged by size: 985 653 228 PvuII CAG'CTG Cuts at: 0 376 1461 1866 Size: 376 1085 405 Fragments arranged by size: 1085 405 376 SacI G_AGCT'C Cuts at: 0 1331 1866 Size: 1331 535 SacII CC_GC'GG Cuts at: 0 1399 1722 1866 Size: 1399 323 144 Fragments arranged by size: 1399 323 144 SapI GCTCTTCn'nnn_ Cuts at: 0 8 1639 1866 Size: 8 1631 227 Fragments arranged by size: 1631 227 8 ScaI AGT'ACT Cuts at: 0 478 1866 Size: 478 1388 SexAI A'CCwGG_T Cuts at: 0 323 485 1866 Size: 323 162 1381 Fragments arranged by size: 1381 323 162 SfcI C'TryA_G Cuts at: 0 649 877 1866 Size: 649 228 989 Fragments arranged by size: 989 649 228 SfoI GGC'GCC Cuts at: 0 1171 1668 1726 1866 Size: 1171 497 58 140 Fragments arranged by size: 1171 497 140 58 SgrAI Cr'CCGG_yG Cuts at: 0 1826 1866 Size: 1826 40 SmaI CCC'GGG Cuts at: 0 1407 1507 1617 1866 Size: 1407 100 110 249 Fragments arranged by size: 1407 249 110 100 SmlI C'TyrA_G Cuts at: 0 425 472 1866 Size: 425 47 1394 Fragments arranged by size: 1394 425 47 SphI G_CATG'C Cuts at: 0 946 1866 Size: 946 920 StyI C'CwwG_G Cuts at: 0 1186 1866 Size: 1186 680 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1401 1866 Size: 1401 465 TatI w'GTAC_w Cuts at: 0 476 1853 1866 Size: 476 1377 13 Fragments arranged by size: 1377 476 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 223 255 917 949 1866 Size: 223 32 662 32 917 Fragments arranged by size: 917 662 223 32 32 XmaI C'CCGG_G Cuts at: 0 1405 1505 1615 1866 Size: 1405 100 110 251 Fragments arranged by size: 1405 251 110 100 Enzymes that do cut: AarI AcuI AflIII AhdI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SphI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmtI Bpu10I BsaI BsaBI BsaWI BseRI BsmI BsmBI BspEI BsrDI BsrGI BstBI BstZ17I ClaI DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI