Restriction map for
pFN21AE2181
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfaI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI HincII
HindII HpaI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI RsrII SacI SacII SalI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AclI 221 AACGTT AA^CGTT
AcsI 619 AAATTT [GA]^AATT[CT]
AcyI 65 GGCGCC G[GA]^CG[CT]C
AfaI 242 GTAC GT^AC
AflII 127 CTTAAG C^TTAAG
ApoI 619 AAATTT [GA]^AATT[CT]
AvaI 91 CCCGAG C^[CT]CG[GA]G
BanII 38 GGGCTC G[GA]GC[CT]^C
BbeI 65 GGCGCC GGCGC^C
BbsI 657 GAAGAC GAAGAC(2/6)
BbvI 11 GCTGC GCAGC(8/12)
BcnI 73 CCGGG CC^[GC]GG
BfrI 127 CTTAAG C^TTAAG
BmyI 38 GGGCTC G[AGT]GC[ACT]^C
BpuAI 657 GAAGAC GAAGAC(2/6)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaHI 65 GGCGCC G[GA]^CG[CT]C
BsaJI 354 CCTGGG C^C[ACGT][ACGT]GG
BseRI 6 GAGGAG GAGGAG(10/8)
BsiEI 49 CGGCCG CG[GA][CT]^CG
BsmFI 142 GTCCC GGGAC(10/14)
BsoBI 91 CCCGAG C^[CT]CG[GA]G
Bsp1286I 38 GGGCTC G[AGT]GC[ACT]^C
BspHI 524 TCATGA T^CATGA
BssHII 19 GCGCGC G^CGCGC
BstF5I 398 GGATG GGATG(2/0)
DrdI 133 GACTGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 49 CGGCCG [CT]^GGCC[GA]
EagI 49 CGGCCG C^GGCCG
EarI 328 CTCTTC CTCTTC(1/4)
EclXI 49 CGGCCG C^GGCCG
Eco52I 49 CGGCCG C^GGCCG
FokI 398 GGATG GGATG(9/13)
HaeII 65 GGCGCC [GA]GCGC^[CT]
Hin1I 65 GGCGCC G[GA]^CG[CT]C
HphI 332 TCACC GGTGA(8/7)
KasI 65 GGCGCC G^GCGCC
Ksp632I 328 CTCTTC CTCTTC(1/4)
MaeIII 315 GTCAC ^GT[ACGT]AC
MseI 128 TTAA T^TAA
MslI 454 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 153 CAGCTG C[AC]G^C[GT]G
NarI 65 GGCGCC GG^CGCC
NciI 73 CCGGG CC^[GC]GG
PpuMI 164 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 221 AACGTT AA^CGTT
PvuII 153 CAGCTG CAG^CTG
RcaI 524 TCATGA T^CATGA
RsaI 242 GTAC GT^AC
SapI 327 GCTCTTC GCTCTTC(1/4)
SfaNI 481 GATGC GCATC(5/9)
SfoI 65 GGCGCC GGC^GCC
SmlI 127 CTTAAG C^T[CT][GA]AG
Tru9I 128 TTAA T^TAA
TseI 11 GCTGC G^C[AT]GC
Tsp45I 315 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Asp700I 118 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
253 GAATGGATTC
AvaII 165 GGACC G^G[AT]CC
436 GGACC
BanI 65 GGCGCC G^G[CT][GA]CC
460 GGTGCC
BbvCI 3 GCTGAGG CCTCAGC(-5/-2)
96 GCTGAGG
BsrDI 465 CATTGC GCAATG(2/0)
502 GCAATG
BsrFI 47 GCCGGC [GA]^CCGG[CT]
62 GCCGGC
BsrI 55 CCAGT ACTGG(1/-1)
186 CCAGT
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
62 GCCGGC
DraII 164 AGGACCT [GA]G^G[ACGT]CC[CT]
420 AGGCCCT
EcoO109I 164 AGGACCT [GA]G^G[ACGT]CC[CT]
420 AGGCCCT
NaeI 47 GCCGGC GCC^GGC
62 GCCGGC
NgoMIV 47 GCCGGC G^CCGGC
62 GCCGGC
Tsp509I 547 AATT ^AATT
620 AATT
XmnI 118 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
253 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AluI BsiYI BslI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII ItaI MaeII MboII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV Sau96I ScrFI TfiI