(Circular) (Six-base) MAPSORT of: pFN21AE2181.seq Check: 1609 from: 1 to: 5425 pFN21AE2181 5425 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2746 2746 Size: 5425 AclI AA'CG_TT Cuts at: 1563 2227 3552 3925 1563 Size: 664 1325 373 3063 Fragments arranged by size: 3063 1325 664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3488 4872 5140 1632 Size: 1856 1384 268 1917 Fragments arranged by size: 1917 1856 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2132 820 Size: 197 1115 4113 Fragments arranged by size: 4113 1115 197 AflIII A'CryG_T Cuts at: 1203 4324 5301 1203 Size: 3121 977 1327 Fragments arranged by size: 3121 1327 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5425 AlwNI CAG_nnn'CTG Cuts at: 3168 4740 3168 Size: 1572 3853 ApaLI G'TGCA_C Cuts at: 3482 4638 3482 Size: 1156 4269 ApoI r'AATT_y Cuts at: 1493 2624 2734 2960 3112 5221 1493 Size: 1131 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 1131 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2750 1870 Size: 73 153 654 4545 Fragments arranged by size: 4545 654 153 73 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2465 2746 4201 618 Size: 325 217 627 283 395 281 1455 1842 Fragments arranged by size: 1842 1455 627 395 325 283 281 217 BanII G_rGCy'C Cuts at: 1391 2047 2744 1391 Size: 656 697 4072 Fragments arranged by size: 4072 697 656 BbeI G_GCGC'C Cuts at: 2074 2074 Size: 5425 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2669 928 Size: 971 770 3684 Fragments arranged by size: 3684 971 770 BbvCI CC'TCA_GC Cuts at: 2009 2102 2009 Size: 93 5332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3616 3650 1903 Size: 34 50 34 1595 34 3678 Fragments arranged by size: 3678 1595 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3317 4527 3317 Size: 1210 4215 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4036 136 Size: 107 122 71 631 605 2364 1525 Fragments arranged by size: 2364 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5420 5420 Size: 5425 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 3486 4642 1790 Size: 1696 1156 2573 Fragments arranged by size: 2573 1696 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4114 442 Size: 416 53 83 399 24 1739 958 1753 Fragments arranged by size: 1753 1739 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4085 1239 Size: 2846 2579 Bpu10I CC'TnA_GC Cuts at: 2009 2102 2009 Size: 93 5332 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3550 4415 4713 3550 Size: 865 298 4262 Fragments arranged by size: 4262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4088 882 Size: 617 2589 2219 Fragments arranged by size: 2589 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3216 493 Size: 711 1170 842 2702 Fragments arranged by size: 2702 1170 842 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2071 3232 3614 4249 Size: 53 83 186 936 538 1161 382 635 Cuts at: 4249 275 Size: 1451 Fragments arranged by size: 1451 1161 936 635 538 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3858 4256 4530 4677 5088 3858 Size: 398 274 147 411 4195 Fragments arranged by size: 4195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5200 Fragments arranged by size: 5200 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 1186 Size: 840 4585 BseYI C'CCAG_C Cuts at: 2038 2156 2328 2516 4628 5124 2038 Size: 118 172 188 2112 496 2339 Fragments arranged by size: 2339 2112 496 188 172 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4852 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2057 2875 3636 4664 1540 Size: 275 186 56 818 761 1028 2301 Fragments arranged by size: 2301 1028 818 761 275 186 56 BsiHKAI G_wGCw'C Cuts at: 3486 3571 4642 3486 Size: 85 1071 4269 Fragments arranged by size: 4269 1071 85 BsmI GAATG_Cn' Cuts at: 2936 3029 2936 Size: 93 5332 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2744 3486 3571 4642 1391 Size: 399 257 697 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 697 399 257 85 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 2529 3314 5044 2529 Size: 785 1730 2910 Fragments arranged by size: 2910 1730 785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2780 844 Size: 1936 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2469 2514 3920 4094 65 Size: 1183 1221 45 1406 174 1396 Fragments arranged by size: 1406 1396 1221 1183 174 45 BsrFI r'CCGG_y Cuts at: 1700 1810 2052 2067 4069 4256 1700 Size: 110 242 15 2002 187 2869 Fragments arranged by size: 2869 2002 242 187 110 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5425 BssHII G'CGCG_C Cuts at: 1925 2024 1925 Size: 99 5326 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5425 BstBI TT'CG_AA Cuts at: 4229 4229 Size: 5425 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5425 BstYI r'GATC_y Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420 Size: 783 367 385 17 1452 86 12 346 Cuts at: 5420 1972 Size: 1977 Fragments arranged by size: 1977 1452 783 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4873 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2941 3735 3755 2941 Size: 794 20 4611 Fragments arranged by size: 4611 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2144 4432 809 Size: 507 828 2288 1802 Fragments arranged by size: 2288 1802 828 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2872 3761 Size: 54 1425 50 275 18 224 818 889 Cuts at: 3761 5390 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 818 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2054 2872 1537 Size: 275 242 818 4090 Fragments arranged by size: 4090 818 275 242 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 3355 1353 Size: 986 1016 3423 Fragments arranged by size: 3423 1016 986 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4012 4386 4532 387 Size: 1064 138 2423 374 146 1280 Fragments arranged by size: 2423 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3488 4085 4872 5140 1239 Size: 393 1856 597 787 268 1524 Fragments arranged by size: 1856 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2170 2426 2170 Size: 256 5169 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2147 2147 Size: 5425 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 2074 4572 1678 Size: 396 2498 2531 Fragments arranged by size: 2531 2498 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2524 2556 4066 Size: 32 135 32 518 32 402 32 1510 Cuts at: 4066 4098 4140 4172 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1510 518 402 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 2297 2344 2686 748 Size: 1549 47 342 3487 Fragments arranged by size: 3487 1549 342 47 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KasI G'GCGC_C Cuts at: 2070 2070 Size: 5425 KpnI G_GTAC'C Cuts at: 2750 2750 Size: 5425 MfeI C'AATT_G Cuts at: 3024 3024 Size: 5425 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4539 4723 1305 Size: 3234 184 2007 Fragments arranged by size: 3234 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3603 Fragments arranged by size: 3603 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 3383 3742 3901 518 Size: 648 1297 920 359 159 2042 Fragments arranged by size: 2042 1297 920 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2841 3518 4666 4911 1606 Size: 554 681 677 1148 245 2120 Fragments arranged by size: 2120 1148 681 677 554 245 NaeI GCC'GGC Cuts at: 2054 2069 2054 Size: 15 5410 NarI GG'CG_CC Cuts at: 2071 2071 Size: 5425 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4873 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NgoMIV G'CCGG_C Cuts at: 2052 2067 2052 Size: 15 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2783 4328 1132 Size: 97 1554 1545 2229 Fragments arranged by size: 2229 1554 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5425 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5425 PfoI T'CCnGG_A Cuts at: 2219 2219 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2170 Size: 5425 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5425 PsiI TTA'TAA Cuts at: 2556 2696 2995 2556 Size: 140 299 4986 Fragments arranged by size: 4986 299 140 PstI C_TGCA'G Cuts at: 830 2777 830 Size: 1947 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 PvuII CAG'CTG Cuts at: 2160 2160 Size: 5425 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SapI GCTCTTCn'nnn_ Cuts at: 2339 2339 Size: 5425 SbfI CC_TGCA'GG Cuts at: 2777 2777 Size: 5425 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SfcI C'TryA_G Cuts at: 826 1045 2773 3908 4589 4780 826 Size: 219 1728 1135 681 191 1471 Fragments arranged by size: 1728 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2072 2072 Size: 5425 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2752 1872 Size: 880 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 3529 4430 4692 820 Size: 197 926 189 1397 901 262 1553 Fragments arranged by size: 1553 1397 926 901 262 197 189 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2783 1132 Size: 1651 3774 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5425 StyI C'CwwG_G Cuts at: 513 1065 2864 513 Size: 552 1799 3074 Fragments arranged by size: 3074 1799 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3452 3469 3622 3807 4252 325 Size: 1255 1872 17 153 185 445 1498 Fragments arranged by size: 1872 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3671 5383 96 Size: 274 80 33 51 1443 1694 1712 138 Fragments arranged by size: 1712 1694 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5385 5417 5385 Size: 32 5393 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5425 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5425 XmaI C'CCGG_G Cuts at: 1870 2750 1870 Size: 880 4545 XmnI GAAnn'nnTTC Cuts at: 2127 2262 3554 2127 Size: 135 1292 3998 Fragments arranged by size: 3998 1292 135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI