Restriction map for
pFN21AE2181
(Linear) (Six-base) MAPSORT of: KIEE2181.seq Check: 1290 from: 1 to: 720
KIEE2181.orf
With 182 enzymes: SgfI *
July 3, 2012 17:11 ..
AclI AA'CG_TT
Cuts at: 0 222 720
Size: 222 498
AflII C'TTAA_G
Cuts at: 0 127 720
Size: 127 593
ApoI r'AATT_y
Cuts at: 0 619 720
Size: 619 101
AvaI C'yCGr_G
Cuts at: 0 91 720
Size: 91 629
BanI G'GyrC_C
Cuts at: 0 65 460 720
Size: 65 395 260
Fragments arranged by size:
395 260 65
BanII G_rGCy'C
Cuts at: 0 42 720
Size: 42 678
BbeI G_GCGC'C
Cuts at: 0 69 720
Size: 69 651
BbsI GAAGACnn'nnnn_
Cuts at: 0 664 720
Size: 664 56
BbvCI CC'TCA_GC
Cuts at: 0 4 97 720
Size: 4 93 623
Fragments arranged by size:
623 93 4
Bpu10I CC'TnA_GC
Cuts at: 0 4 97 720
Size: 4 93 623
Fragments arranged by size:
623 93 4
BsaAI yAC'GTr
Cuts at: 0 369 720
Size: 369 351
BsaHI Gr'CG_yC
Cuts at: 0 66 720
Size: 66 654
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 720
Size: 21 699
BseYI C'CCAG_C
Cuts at: 0 33 151 323 511 720
Size: 33 118 172 188 209
Fragments arranged by size:
209 188 172 118 33
BsiEI CG_ry'CG
Cuts at: 0 52 720
Size: 52 668
Bsp1286I G_dGCh'C
Cuts at: 0 42 720
Size: 42 678
BspHI T'CATG_A
Cuts at: 0 524 720
Size: 524 196
BsrDI GCAATG_nn'
Cuts at: 0 464 509 720
Size: 464 45 211
Fragments arranged by size:
464 211 45
BsrFI r'CCGG_y
Cuts at: 0 47 62 720
Size: 47 15 658
Fragments arranged by size:
658 47 15
BssHII G'CGCG_C
Cuts at: 0 19 720
Size: 19 701
DrdI GACnn_nn'nnGTC
Cuts at: 0 139 720
Size: 139 581
EaeI y'GGCC_r
Cuts at: 0 49 720
Size: 49 671
EagI C'GGCC_G
Cuts at: 0 49 720
Size: 49 671
EarI CTCTTCn'nnn_
Cuts at: 0 334 720
Size: 334 386
EcoO109I rG'GnC_Cy
Cuts at: 0 165 421 720
Size: 165 256 299
Fragments arranged by size:
299 256 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 142 720
Size: 142 578
HaeII r_GCGC'y
Cuts at: 0 69 720
Size: 69 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 519 551 720
Size: 85 32 402 32 169
Fragments arranged by size:
402 169 85 32 32
HindIII A'AGCT_T
Cuts at: 0 292 339 681 720
Size: 292 47 342 39
Fragments arranged by size:
342 292 47 39
KasI G'GCGC_C
Cuts at: 0 65 720
Size: 65 655
MslI CAynn'nnrTG
Cuts at: 0 458 720
Size: 458 262
MspA1I CmG'CkG
Cuts at: 0 155 720
Size: 155 565
NaeI GCC'GGC
Cuts at: 0 49 64 720
Size: 49 15 656
Fragments arranged by size:
656 49 15
NarI GG'CG_CC
Cuts at: 0 66 720
Size: 66 654
NgoMIV G'CCGG_C
Cuts at: 0 47 62 720
Size: 47 15 658
Fragments arranged by size:
658 47 15
PfoI T'CCnGG_A
Cuts at: 0 214 720
Size: 214 506
PpuMI rG'GwC_Cy
Cuts at: 0 165 720
Size: 165 555
PsiI TTA'TAA
Cuts at: 0 551 691 720
Size: 551 140 29
Fragments arranged by size:
551 140 29
PvuII CAG'CTG
Cuts at: 0 155 720
Size: 155 565
SapI GCTCTTCn'nnn_
Cuts at: 0 334 720
Size: 334 386
SfoI GGC'GCC
Cuts at: 0 67 720
Size: 67 653
SmlI C'TyrA_G
Cuts at: 0 127 720
Size: 127 593
XmnI GAAnn'nnTTC
Cuts at: 0 122 257 720
Size: 122 135 463
Fragments arranged by size:
463 135 122
Enzymes that do cut:
AclI AflII ApoI AvaI BanI BanII BbeI BbsI
BbvCI Bpu10I BsaAI BsaHI BseRI BseYI BsiEI Bsp1286I
BspHI BsrDI BsrFI BssHII DrdI EaeI EagI EarI
EcoO109I FalI HaeII Hin4I HindIII KasI MslI MspA1I
NaeI NarI NgoMIV PfoI PpuMI PsiI PvuII SapI
SfoI SmlI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI