################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 762 CGCG CG^CG AcsI 382 AAATTC [GA]^AATT[CT] AfaI 540 GTAC GT^AC ApoI 382 AAATTC [GA]^AATT[CT] BamHI 45 GGATCC G^GATCC BanII 137 GGGCTC G[GA]GC[CT]^C BcgI 468 CGAGGGCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 858 CCGGG CC^[GC]GG BglII 423 AGATCT A^GATCT BmyI 137 GGGCTC G[AGT]GC[ACT]^C BsgI 841 GTGCAG GTGCAG(16/14) BsmAI 610 GAGAC GTCTC(1/5) BsmBI 610 GAGACG CGTCTC(1/5) Bsp1286I 137 GGGCTC G[AGT]GC[ACT]^C BspMI 615 GCAGGT ACCTGC(4/8) BssHII 761 GCGCGC G^CGCGC BstUI 762 CGCG CG^CG BstXI 548 CCAAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 786 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 282 CCATGG C^C[GA][CT]GG EcoO109I 786 AGGCCCC [GA]G^G[ACGT]CC[CT] HaeII 520 AGCGCC [GA]GCGC^[CT] HapII 858 CCGG C^CGG HpaII 858 CCGG C^CGG MaeIII 677 GTTAC ^GT[ACGT]AC MslI 238 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 91 CAGCGG C[AC]G^C[GT]G MspI 858 CCGG C^CGG MvnI 762 CGCG CG^CG NciI 858 CCGGG CC^[GC]GG NcoI 282 CCATGG C^CATGG PflMI 255 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 540 GTAC GT^AC ScaI 539 AGTACT AGT^ACT SmlI 337 CTTGAG C^T[CT][GA]AG StuI 812 AGGCCT AGG^CCT Tsp509I 383 AATT ^AATT Van91I 255 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 256 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 593 CAGGGTCTG AocI 688 CCTAAGG CC^T[ACGT]AGG 850 CCTGAGG AvaI 466 CCCGAG C^[CT]CG[GA]G 718 CCCGAG AvaII 798 GGACC G^G[AT]CC 834 GGACC BalI 87 TGGCCA TGG^CCA 746 TGGCCA BbsI 220 GAAGAC GAAGAC(2/6) 459 GAAGAC BpuAI 220 GAAGAC GAAGAC(2/6) 459 GAAGAC BseRI 496 GAGGAG GAGGAG(10/8) 885 GAGGAG BsoBI 466 CCCGAG C^[CT]CG[GA]G 718 CCCGAG Bsu36I 688 CCTAAGG CC^T[ACGT]AGG 850 CCTGAGG EarI 756 GAAGAG CTCTTC(1/4) 772 GAAGAG Eco81I 688 CCTAAGG CC^T[ACGT]AGG 850 CCTGAGG EcoT14I 282 CCATGG C^C[AT][AT]GG 809 CCAAGG HgaI 612 GACGC GACGC(5/10) 931 GACGC HphI 527 TCACC GGTGA(8/7) 866 TCACC Ksp632I 756 GAAGAG CTCTTC(1/4) 772 GAAGAG MaeII 18 ACGT A^CGT 624 ACGT MluNI 87 TGGCCA TGG^CCA 746 TGGCCA MscI 87 TGGCCA TGG^CCA 746 TGGCCA MseI 167 TTAA T^TAA 251 TTAA MwoI 820 GCTCAGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 925 GCAGAGGACGC NlaIII 283 CATG CATG^ 838 CATG PleI 197 GACTC GAGTC(4/5) 462 GACTC SapI 756 GAAGAGC GCTCTTC(1/4) 772 GAAGAGC StyI 282 CCATGG C^C[AT][AT]GG 809 CCAAGG Tru9I 167 TTAA T^TAA 251 TTAA TspRI 57 CACTG CA[GC]TG[ACGT][ACGT]^ 563 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AlwI BbvI BfaI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeI MboI MboII MflI MnlI MvaI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI XhoII