(Circular) (Six-base) MAPSORT of: pFN21AE2125.seq Check: 9901 from: 1 to: 5689 pFN21AE2125 5689 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2611 2611 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 2897 3752 5136 5404 1632 Size: 959 306 855 1384 268 1917 Fragments arranged by size: 1917 1384 959 855 306 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2266 2603 3432 5004 2266 Size: 337 829 1572 2951 Fragments arranged by size: 2951 1572 829 337 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2387 2998 3224 3376 5485 1493 Size: 894 611 226 152 2109 1697 Fragments arranged by size: 2109 1697 894 611 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2471 2723 3014 1870 Size: 73 528 252 291 4545 Fragments arranged by size: 4545 528 291 252 73 BamHI G'GATC_C Cuts at: 2050 3019 3386 2050 Size: 969 367 4353 Fragments arranged by size: 4353 969 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2146 3008 1391 Size: 755 862 4072 Fragments arranged by size: 4072 862 755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 2471 928 Size: 971 333 239 4146 Fragments arranged by size: 4146 971 333 239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2462 2496 3880 3914 1903 Size: 34 525 34 1384 34 3678 Fragments arranged by size: 3678 1384 525 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2428 5684 2428 Size: 3256 2433 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 3750 4906 1790 Size: 1960 1156 2573 Fragments arranged by size: 2573 1960 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2481 2672 4349 1239 Size: 850 392 191 1677 2579 Fragments arranged by size: 2579 1677 850 392 191 Bpu10I CC'TnA_GC Cuts at: 2145 2826 2145 Size: 681 5008 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 3814 4679 4977 2363 Size: 1451 865 298 3075 Fragments arranged by size: 3075 1451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 2905 1186 Size: 1330 389 3970 Fragments arranged by size: 3970 1330 389 BseYI C'CCAG_C Cuts at: 2799 4892 5388 2799 Size: 2093 496 3100 Fragments arranged by size: 3100 2093 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2867 1163 Size: 573 1131 3985 Fragments arranged by size: 3985 1131 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3750 3835 4906 3750 Size: 85 1071 4533 Fragments arranged by size: 4533 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 BsmBI CGTCTCn'nnnn_ Cuts at: 2609 2609 Size: 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 3008 3750 3835 4906 1391 Size: 399 356 862 742 85 1071 2174 Fragments arranged by size: 2174 1071 862 742 399 356 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2611 3044 844 Size: 1767 433 3489 Fragments arranged by size: 3489 1767 433 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 2766 1925 Size: 841 4848 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2560 2560 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2050 2404 2428 3019 3386 3771 3788 5240 Size: 78 354 24 591 367 385 17 1452 Cuts at: 5240 5326 5338 5684 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 591 385 367 354 346 86 78 24 17 12 Bsu36I CC'TnA_GG Cuts at: 2694 2856 2694 Size: 162 5527 BtgI C'CryG_G Cuts at: 513 1065 2287 513 Size: 552 1222 3915 Fragments arranged by size: 3915 1222 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2493 487 Size: 683 56 1267 3683 Fragments arranged by size: 3683 1267 683 56 BtsI GCAGTG_nn' Cuts at: 2061 3205 3999 4019 2061 Size: 1144 794 20 3731 Fragments arranged by size: 3731 1144 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2268 2751 Size: 54 1425 50 275 18 262 176 483 Cuts at: 2751 3136 4025 5654 8 Size: 385 889 1629 43 Fragments arranged by size: 1629 1425 889 483 385 275 262 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2756 2772 3619 1353 Size: 1403 16 847 3423 Fragments arranged by size: 3423 1403 847 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 4276 4650 4796 387 Size: 1064 138 455 2232 374 146 1280 Fragments arranged by size: 2232 1280 1064 455 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2481 2591 2672 2897 3752 4349 Size: 393 457 392 110 81 225 855 597 Cuts at: 4349 5136 5404 1239 Size: 787 268 1524 Fragments arranged by size: 1524 855 787 597 457 393 392 268 225 110 81 EcoO109I rG'GnC_Cy Cuts at: 2792 2792 Size: 5689 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2529 4836 1678 Size: 851 2307 2531 Fragments arranged by size: 2531 2307 851 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2199 2227 2231 2259 2336 Size: 32 135 32 627 28 4 28 77 Cuts at: 2336 2368 2396 2428 4330 4362 4404 4436 1373 Size: 32 28 32 1902 32 42 32 2626 Fragments arranged by size: 2626 1902 627 135 77 42 32 32 32 32 32 32 28 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2094 2753 10 Size: 54 1425 343 262 659 2946 Fragments arranged by size: 2946 1425 659 343 262 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2247 3647 4006 4165 518 Size: 648 843 238 1400 359 159 2042 Fragments arranged by size: 2042 1400 843 648 359 238 159 MspA1I CmG'CkG Cuts at: 1606 2098 3105 3782 4930 5175 1606 Size: 492 1007 677 1148 245 2120 Fragments arranged by size: 2120 1148 1007 677 492 245 NcoI C'CATG_G Cuts at: 513 1065 2287 513 Size: 552 1222 3915 Fragments arranged by size: 3915 1222 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2266 1108 Size: 1158 4531 PfoI T'CCnGG_A Cuts at: 2538 2538 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2756 2772 2756 Size: 16 5673 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2546 3937 5649 2546 Size: 1391 1712 2586 Fragments arranged by size: 2586 1712 1391 SfcI C'TryA_G Cuts at: 826 1045 3037 4172 4853 5044 826 Size: 219 1992 1135 681 191 1471 Fragments arranged by size: 1992 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 3793 4694 4956 820 Size: 197 926 399 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 399 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2819 1517 Size: 1302 4387 StyI C'CwwG_G Cuts at: 513 1065 2287 2814 3128 513 Size: 552 1222 527 314 3074 Fragments arranged by size: 3074 1222 552 527 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 3935 5647 Size: 274 80 33 51 1443 567 1391 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1391 567 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI