(Linear) (Six-base) MAPSORT of: KIEE2125.seq Check: 160 from: 1 to: 984 KIEE2125.orf With 182 enzymes: SgfI * July 3, 2012 17:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 606 984 Size: 606 378 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 892 984 Size: 586 306 92 Fragments arranged by size: 586 306 92 AlwNI CAG_nnn'CTG Cuts at: 0 261 598 984 Size: 261 337 386 Fragments arranged by size: 386 337 261 ApoI r'AATT_y Cuts at: 0 382 984 Size: 382 602 AvaI C'yCGr_G Cuts at: 0 466 718 984 Size: 466 252 266 Fragments arranged by size: 466 266 252 BamHI G'GATC_C Cuts at: 0 45 984 Size: 45 939 BanII G_rGCy'C Cuts at: 0 141 984 Size: 141 843 BbsI GAAGACnn'nnnn_ Cuts at: 0 227 466 984 Size: 227 239 518 Fragments arranged by size: 518 239 227 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 457 491 984 Size: 457 34 493 Fragments arranged by size: 493 457 34 BglII A'GATC_T Cuts at: 0 423 984 Size: 423 561 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 476 667 984 Size: 84 392 191 317 Fragments arranged by size: 392 317 191 84 Bpu10I CC'TnA_GC Cuts at: 0 140 821 984 Size: 140 681 163 Fragments arranged by size: 681 163 140 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 984 Size: 358 626 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 511 900 984 Size: 511 389 84 Fragments arranged by size: 511 389 84 BseYI C'CCAG_C Cuts at: 0 794 984 Size: 794 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 984 Size: 862 122 BsmBI CGTCTCn'nnnn_ Cuts at: 0 604 984 Size: 604 380 Bsp1286I G_dGCh'C Cuts at: 0 141 984 Size: 141 843 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 606 984 Size: 606 378 BssHII G'CGCG_C Cuts at: 0 761 984 Size: 761 223 BstXI CCAn_nnnn'nTGG Cuts at: 0 555 984 Size: 555 429 BstYI r'GATC_y Cuts at: 0 45 399 423 984 Size: 45 354 24 561 Fragments arranged by size: 561 354 45 24 Bsu36I CC'TnA_GG Cuts at: 0 689 851 984 Size: 689 162 133 Fragments arranged by size: 689 162 133 BtgI C'CryG_G Cuts at: 0 282 984 Size: 282 702 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 488 984 Size: 488 496 BtsI GCAGTG_nn' Cuts at: 0 56 984 Size: 56 928 EaeI y'GGCC_r Cuts at: 0 87 263 746 984 Size: 87 176 483 238 Fragments arranged by size: 483 238 176 87 EarI CTCTTCn'nnn_ Cuts at: 0 751 767 984 Size: 751 16 217 Fragments arranged by size: 751 217 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 39 984 Size: 39 945 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 476 586 667 892 984 Size: 84 392 110 81 225 92 Fragments arranged by size: 392 225 110 92 84 81 EcoO109I rG'GnC_Cy Cuts at: 0 787 984 Size: 787 197 HaeII r_GCGC'y Cuts at: 0 524 984 Size: 524 460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 194 222 226 254 331 363 391 423 Size: 194 28 4 28 77 32 28 32 Cuts at: 423 984 Size: 561 Fragments arranged by size: 561 194 77 32 32 28 28 28 4 MscI TGG'CCA Cuts at: 0 89 748 984 Size: 89 659 236 Fragments arranged by size: 659 236 89 MslI CAynn'nnrTG Cuts at: 0 242 984 Size: 242 742 MspA1I CmG'CkG Cuts at: 0 93 984 Size: 93 891 NcoI C'CATG_G Cuts at: 0 282 984 Size: 282 702 PflMI CCAn_nnn'nTGG Cuts at: 0 261 984 Size: 261 723 PfoI T'CCnGG_A Cuts at: 0 533 984 Size: 533 451 SapI GCTCTTCn'nnn_ Cuts at: 0 751 767 984 Size: 751 16 217 Fragments arranged by size: 751 217 16 ScaI AGT'ACT Cuts at: 0 541 984 Size: 541 443 SmlI C'TyrA_G Cuts at: 0 337 984 Size: 337 647 StuI AGG'CCT Cuts at: 0 814 984 Size: 814 170 StyI C'CwwG_G Cuts at: 0 282 809 984 Size: 282 527 175 Fragments arranged by size: 527 282 175 TatI w'GTAC_w Cuts at: 0 539 984 Size: 539 445 Enzymes that do cut: AarI AcuI AlwNI ApoI AvaI BamHI BanII BbsI BcgI BglII BpmI Bpu10I BpuEI BseRI BseYI BsgI BsmBI Bsp1286I BspMI BssHII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I MscI MslI MspA1I NcoI PflMI PfoI SapI ScaI SmlI StuI StyI TatI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI