Restriction map for
pFN21AE2224
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstUI BstXI BstYI CelII
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
FauI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI MwoI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1128 GTATAC GT^[AC][GT]AC
AflIII 438 ACATGT A^C[GA][CT]GT
AgeI 1122 ACCGGT A^CCGGT
AocI 247 CCTGAGG CC^T[ACGT]AGG
Asp700I 956 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 698 GTGCTC G[AT]GC[AT]^C
BbsI 1103 GAAGAC GAAGAC(2/6)
BbvI 1078 GCAGC GCAGC(8/12)
BpuAI 1103 GAAGAC GAAGAC(2/6)
BsaI 1084 GAGACC GGTCTC(1/5)
BsaWI 1122 ACCGGT [AT]^CCGG[AT]
BsiHKAI 698 GTGCTC G[AT]GC[AT]^C
BsmI 158 GCATTC GAATGC(1/-1)
Bsp1407I 257 TGTACA T^GTACA
BspLU11I 438 ACATGT A^CATGT
BsrFI 1122 ACCGGT [GA]^CCGG[CT]
BsrGI 257 TGTACA T^GTACA
Bst1107I 1128 GTATAC GTA^TAC
BstEII 1118 GGTTACC G^GT[ACGT]ACC
BstPI 1118 GGTTACC G^GT[ACGT]ACC
BstZ17I 1128 GTATAC GTA^TAC
Bsu36I 247 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1122 ACCGGT [GA]^CCGG[CT]
DraI 423 TTTAAA TTT^AAA
DsaI 627 CCATGG C^C[GA][CT]GG
Eco81I 247 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1118 GGTTACC G^GT[ACGT]ACC
EcoT22I 869 ATGCAT ATGCA^T
Fnu4HI 1078 GCAGC GC^[ACGT]GC
HindIII 1201 AAGCTT A^AGCTT
ItaI 1078 GCAGC GC^[ACGT]GC
MspA1I 1079 CAGCTG C[AC]G^C[GT]G
NcoI 627 CCATGG C^CATGG
NsiI 869 ATGCAT ATGCA^T
PflMI 664 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1122 ACCGGT A^CCGGT
PvuII 1079 CAGCTG CAG^CTG
SmlI 1032 CTTGAG C^T[CT][GA]AG
SpeI 1107 ACTAGT A^CTAGT
SspBI 257 TGTACA T^GTACA
TseI 1078 GCAGC G^C[AT]GC
Van91I 664 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 956 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 63 CCGC CCGC(-3/-1)
433 CCGC
AfaI 14 GTAC GT^AC
258 GTAC
AlwI 661 GATCC GGATC(4/5)
922 GATCC
AvaII 793 GGACC G^G[AT]CC
1266 GGACC
BanI 1155 GGTGCC G^G[CT][GA]CC
1163 GGCACC
BclI 1229 TGATCA T^GATCA
1290 TGATCA
BfaI 770 CTAG C^TAG
1108 CTAG
BmyI 698 GTGCTC G[AGT]GC[ACT]^C
894 GGGCAC
BpmI 55 CTGGAG CTGGAG(16/14)
885 CTGGAG
BseRI 34 CTCCTC GAGGAG(10/8)
250 GAGGAG
BsmAI 570 GAGAC GTCTC(1/5)
1084 GAGAC
BsmFI 724 GGGAC GGGAC(10/14)
1265 GGGAC
Bsp1286I 698 GTGCTC G[AGT]GC[ACT]^C
894 GGGCAC
BsrI 1208 CCAGT ACTGG(1/-1)
1269 CCAGT
BstF5I 483 CATCC GGATG(2/0)
597 GGATG
EarI 364 GAAGAG CTCTTC(1/4)
1147 GAAGAG
FbaI 1229 TGATCA T^GATCA
1290 TGATCA
FokI 483 CATCC GGATG(9/13)
597 GGATG
HaeII 3 GGCGCT [GA]GCGC^[CT]
1223 GGCGCT
HaeIII 682 GGCC GG^CC
813 GGCC
HapII 70 CCGG C^CGG
1123 CCGG
HpaII 70 CCGG C^CGG
1123 CCGG
HphI 1245 GGTGA GGTGA(8/7)
139 TCACC
Ksp632I 364 GAAGAG CTCTTC(1/4)
1147 GAAGAG
MaeI 770 CTAG C^TAG
1108 CTAG
MslI 703 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1042 CATCTGAGTG
MspI 70 CCGG C^CGG
1123 CCGG
NdeI 713 CATATG CA^TATG
929 CATATG
NspI 438 ACATGT [GA]CATG^[CT]
694 GCATGT
PleI 313 GACTC GAGTC(4/5)
550 GAGTC
RsaI 14 GTAC GT^AC
258 GTAC
SspI 502 AATATT AAT^ATT
1334 AATATT
Tsp45I 138 GTCAC ^GT[GC]AC
1211 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsiYI
BslI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
HhaI HinP1I HinfI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I Tsp509I
TspRI