Restriction map for
pFN21AE2224
(Circular) (Six-base) MAPSORT of: pFN21AE2224.seq Check: 5472 from: 1 to: 6094
pFN21AE2224 6094 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 3437 1937
Size: 1197 303 4594
Fragments arranged by size:
4594 1197 303
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 3171 4157 5541 5809 1632
Size: 561 978 986 1384 268 1917
Fragments arranged by size:
1917 1384 986 978 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2443 4993 5970 1203
Size: 1240 2550 977 1327
Fragments arranged by size:
2550 1327 1240 977
AgeI A'CCGG_T
Cuts at: 3127 4925 3127
Size: 1798 4296
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 3837 5409 3837
Size: 1572 4522
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 2188 2399 2425 3257 3403 3629 3781 5890
Size: 695 211 26 832 146 226 152 2109
Cuts at: 5890 1493
Size: 1697
Fragments arranged by size:
2109 1697 832 695 226 211 152 146
26
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 3419 1870
Size: 73 1476 4545
Fragments arranged by size:
4545 1476 73
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 3168 3415 4870 618
Size: 325 217 627 1373 8 247 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 247 217 8
BanII G_rGCy'C
Cuts at: 1391 3413 1391
Size: 2022 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3115 928
Size: 971 1216 3907
Fragments arranged by size:
3907 1216 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 3234 3295 1548
Size: 1686 61 4347
Fragments arranged by size:
4347 1686 61
BfrBI ATG'CAT
Cuts at: 2876 2876
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4705 136
Size: 107 122 71 631 605 3033 1525
Fragments arranged by size:
3033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2903 4155 5311 1790
Size: 1113 1252 1156 2573
Fragments arranged by size:
2573 1252 1156 1113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3825 4783 442
Size: 416 53 83 399 24 2408 958 1753
Fragments arranged by size:
2408 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2911 4754 1239
Size: 842 830 1843 2579
Fragments arranged by size:
2579 1843 842 830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3058 4219 5084 5382 3058
Size: 1161 865 298 3770
Fragments arranged by size:
3770 1161 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3083 4757 882
Size: 617 1584 1674 2219
Fragments arranged by size:
2219 1674 1584 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3901 4283 4918 275
Size: 53 83 186 936 2368 382 635 1451
Fragments arranged by size:
2368 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3127 4527 4925 5199 5346 5757 3127
Size: 1400 398 274 147 411 3464
Fragments arranged by size:
3464 1400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2914 2944 1178
Size: 30 165 30 1511 30 4328
Fragments arranged by size:
4328 1511 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2270 1186
Size: 844 240 5010
Fragments arranged by size:
5010 844 240
BseYI C'CCAG_C
Cuts at: 5297 5793 5297
Size: 496 5598
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2707 4155 4240 5311 2707
Size: 1448 85 1071 3490
Fragments arranged by size:
3490 1448 1071 85
BsmI GAATG_Cn'
Cuts at: 2163 3605 3698 2163
Size: 1442 93 4559
Fragments arranged by size:
4559 1442 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2707 2903 3413 4155 4240 5311 1391
Size: 399 917 196 510 742 85 1071 2174
Fragments arranged by size:
2174 1071 917 742 510 399 196 85
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3449 844
Size: 2605 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3127 4738 4925 1700
Size: 110 1317 1611 187 2869
Fragments arranged by size:
2869 1611 1317 187 110
BsrGI T'GTAC_A
Cuts at: 96 2262 96
Size: 2166 3928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6094
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 3123 1184
Size: 1939 4155
BstYI r'GATC_y
Cuts at: 1972 3424 3791 4176 4193 5645 5731 5743 6089
Size: 1452 367 385 17 1452 86 12 346
Cuts at: 6089 1972
Size: 1977
Fragments arranged by size:
1977 1452 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3135 3135
Size: 6094
Bsu36I CC'TnA_GG
Cuts at: 2253 2253
Size: 6094
BtgI C'CryG_G
Cuts at: 513 1065 2004 2632 513
Size: 552 939 628 3975
Fragments arranged by size:
3975 939 628 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 3610 4404 4424 3610
Size: 794 20 5280
Fragments arranged by size:
5280 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 2430 3398 2430
Size: 968 5126
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3541 4430 6059
Size: 54 1425 50 275 18 1711 889 1629
Cuts at: 6059 8
Size: 43
Fragments arranged by size:
1711 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 3147 4024 1353
Size: 1011 783 877 3423
Fragments arranged by size:
3423 1011 877 783
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 4681 5055 5201 387
Size: 1064 138 468 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 468 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2193 2911 3171 4157 4754 5541
Size: 393 449 112 718 260 986 597 787
Cuts at: 5541 5809 1239
Size: 268 1524
Fragments arranged by size:
1524 986 787 718 597 449 393 268
260 112
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2012 3232 5241 1678
Size: 334 1220 2009 2531
Fragments arranged by size:
2531 2009 1220 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3031 3063 4735 4767 4809
Size: 32 135 32 1459 32 1672 32 42
Cuts at: 4809 4841 1373
Size: 32 2626
Fragments arranged by size:
2626 1672 1459 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3438 3684 669
Size: 988 281 1500 246 3079
Fragments arranged by size:
3079 1500 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3206 748
Size: 2458 3636
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2656 5208 5392 1305
Size: 1351 2552 184 2007
Fragments arranged by size:
2552 2007 1351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4272
Fragments arranged by size:
4272 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2712 3051 4052 4411 4570 518
Size: 648 1546 339 1001 359 159 2042
Fragments arranged by size:
2042 1546 1001 648 359 339 159
MspA1I CmG'CkG
Cuts at: 1606 3086 3510 4187 5335 5580 1606
Size: 1480 424 677 1148 245 2120
Fragments arranged by size:
2120 1480 1148 677 424 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2632 513
Size: 552 939 628 3975
Fragments arranged by size:
3975 939 628 552
NdeI CA'TA_TG
Cuts at: 387 2719 2935 387
Size: 2332 216 3546
Fragments arranged by size:
3546 2332 216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NsiI A_TGCA'T
Cuts at: 2878 2878
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2447 2703 3452 4997 1132
Size: 97 1218 256 749 1545 2229
Fragments arranged by size:
2229 1545 1218 749 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6094
PciI A'CATG_T
Cuts at: 2443 4993 2443
Size: 2550 3544
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2675 1108
Size: 1567 4527
PfoI T'CCnGG_A
Cuts at: 2888 2888
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PstI C_TGCA'G
Cuts at: 830 3446 830
Size: 2616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 3086 3086
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 3442 4577 5258 5449 826
Size: 219 2397 1135 681 191 1471
Fragments arranged by size:
2397 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3037 4198 5099 5361 820
Size: 197 926 1094 1161 901 262 1553
Fragments arranged by size:
1553 1161 1094 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 3112 152
Size: 2960 3134
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 2509 3341 4018 5
Size: 47 2457 832 677 2081
Fragments arranged by size:
2457 2081 832 677 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6094
StyI C'CwwG_G
Cuts at: 513 1065 2004 2632 3164 3313 3369 3533 513
Size: 552 939 628 532 149 56 164 3074
Fragments arranged by size:
3074 939 628 552 532 164 149 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 4121 4138 4291 4476 4921 325
Size: 1255 1821 720 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 4340 6052
Size: 274 80 33 51 1443 285 2078 1712
Cuts at: 6052 96
Size: 138
Fragments arranged by size:
2078 1712 1443 285 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2194 2226 6054 6086 2194
Size: 32 3828 32 2202
Fragments arranged by size:
3828 2202 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 2965 4223 2965
Size: 1258 4836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI