Restriction map for
pFN21AE2214
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII AvrII BamHI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiHKAI BsiWI
BsmAI BsmBI BspDI BspEI BspHI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MroI MseI MunI NdeI
NheI NsiI NspV PacI PaeR7I
PflFI PflMI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 973 GACGTC GACGT^C
AgeI 561 ACCGGT A^CCGGT
AhdI 673 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 789 GGATC GGATC(4/5)
AocI 1028 CCTCAGG CC^T[ACGT]AGG
AspEI 673 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1372 CACGTG CAC^GTG
BclI 497 TGATCA T^GATCA
BsaAI 1372 CACGTG [CT]AC^GT[GA]
BsaWI 561 ACCGGT [AT]^CCGG[AT]
BsgI 1201 CTGCAC GTGCAG(16/14)
BsmI 231 GCATTC GAATGC(1/-1)
Bsp1407I 1530 TGTACA T^GTACA
BspLU11I 1137 ACATGT A^CATGT
BspMI 1412 ACCTGC ACCTGC(4/8)
BsrGI 1530 TGTACA T^GTACA
BssSI 724 CTCGTG CACGAG(-5/-1)
BstAPI 1176 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 789 GGATCT [GA]^GATC[CT]
Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG
DraIII 1204 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 967 GACTCGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 673 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 937 CTCTTC CTCTTC(1/4)
Eco81I 1028 CCTCAGG CC^T[ACGT]AGG
EcoNI 723 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 497 TGATCA T^GATCA
Ksp632I 937 CTCTTC CTCTTC(1/4)
MflI 789 GGATCT [GA]^GATC[CT]
MslI 1368 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 469 GCGGCCGC GC^GGCCGC
NruI 556 TCGCGA TCG^CGA
NspI 1137 ACATGT [GA]CATG^[CT]
PinAI 561 ACCGGT A^CCGGT
PmaCI 1372 CACGTG CAC^GTG
PmlI 1372 CACGTG CAC^GTG
SfcI 298 CTACAG C^T[GA][CT]AG
SfiI 97 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1530 TGTACA T^GTACA
StuI 1032 AGGCCT AGG^CCT
Tsp45I 1141 GTGAC ^GT[GC]AC
Tsp509I 1191 AATT ^AATT
XhoII 789 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 157 TGGCCA TGG^CCA
1571 TGGCCA
BpmI 846 CTCCAG CTGGAG(16/14)
286 CTGGAG
BstF5I 1368 CATCC GGATG(2/0)
881 GGATG
DpnII 498 GATC ^GATC
790 GATC
EagI 470 CGGCCG C^GGCCG
1158 CGGCCG
EclXI 470 CGGCCG C^GGCCG
1158 CGGCCG
Eco52I 470 CGGCCG C^GGCCG
1158 CGGCCG
FokI 1368 CATCC GGATG(9/13)
881 GGATG
HinfI 892 GAGTC G^A[ACGT]TC
967 GACTC
MaeIII 932 GTAAC ^GT[ACGT]AC
1141 GTGAC
MboI 498 GATC ^GATC
790 GATC
MboII 122 GAAGA GAAGA(8/7)
938 TCTTC
MluI 338 ACGCGT A^CGCGT
1286 ACGCGT
MluNI 157 TGGCCA TGG^CCA
1571 TGGCCA
MscI 157 TGGCCA TGG^CCA
1571 TGGCCA
NcoI 374 CCATGG C^CATGG
448 CCATGG
NdeII 498 GATC ^GATC
790 GATC
PleI 967 GACTC GAGTC(4/5)
892 GAGTC
PvuII 520 CAGCTG CAG^CTG
1575 CAGCTG
Sau3AI 498 GATC ^GATC
790 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI Aor51HI ApaI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI KspI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV RsaI SacII Sau96I
ScrFI SfoI SmaI StyI TaqI
TseI TspRI TthHB8I XmaCI XmaI