Restriction map for
pFN21AE2214
(Circular) (Six-base) MAPSORT of: pFN21AE2214.seq Check: 1167 from: 1 to: 6295
pFN21AE2214 6295 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2982 4105 278
Size: 53 83 186 936 1446 1123 2468
Fragments arranged by size:
2468 1446 1123 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4358 5742 6010 1632
Size: 2726 1384 268 1917
Fragments arranged by size:
2726 1917 1384 268
AfeI AGC'GCT
Cuts at: 2364 2876 3350 2364
Size: 512 474 5309
Fragments arranged by size:
5309 512 474
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 2343 2489 3142 3156 3291 5194 6171 1203
Size: 1140 146 653 14 135 1903 977 1327
Fragments arranged by size:
1903 1327 1140 977 653 146 135 14
AgeI A'CCGG_T
Cuts at: 2566 5126 2566
Size: 2560 3735
AhdI GACnn_n'nnGTC
Cuts at: 2683 5024 2683
Size: 2341 3954
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3311 1184
Size: 2127 4168
AlwNI CAG_nnn'CTG
Cuts at: 2661 3070 3259 4038 5610 2661
Size: 409 189 779 1572 3346
Fragments arranged by size:
3346 1572 779 409 189
ApaI G_GGCC'C
Cuts at: 2114 2742 3329 2114
Size: 628 587 5080
Fragments arranged by size:
5080 628 587
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 3604 3830 3982 6091 1493
Size: 2111 226 152 2109 1697
Fragments arranged by size:
2111 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2087 2114 2262 2617 2866 2914
Size: 73 95 49 27 148 355 249 48
Cuts at: 2914 3111 3620 1870
Size: 197 509 4545
Fragments arranged by size:
4545 509 355 249 197 148 95 73
49 48 27
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2143 2319 2386 2433
Size: 325 217 627 284 72 176 67 47
Cuts at: 2433 3187 3310 3365 3468 3516 3616 5071 618
Size: 754 123 55 103 48 100 1455 1842
Fragments arranged by size:
1842 1455 754 627 325 284 217 176
123 103 100 72 67 55 48 47
BanII G_rGCy'C
Cuts at: 1391 2114 2399 2595 2742 3204 3329 3614 1391
Size: 723 285 196 147 462 125 285 4072
Fragments arranged by size:
4072 723 462 285 285 196 147 125
BbeI G_GCGC'C
Cuts at: 2075 2437 3369 3472 2075
Size: 362 932 103 4898
Fragments arranged by size:
4898 932 362 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2183 2384
Size: 107 122 71 631 605 437 74 201
Cuts at: 2384 3021 4906 136
Size: 637 1885 1525
Fragments arranged by size:
1885 1525 637 631 605 437 201 122
107 74 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2148 2389 2742 3192 3329 3360 4356
Size: 324 34 241 353 450 137 31 996
Cuts at: 4356 5512 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 996 450 353 324 241 137
34 31
BmgBI CAC'GTC
Cuts at: 1318 2490 3157 1318
Size: 1172 667 4456
Fragments arranged by size:
4456 1172 667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3103 4026 4984
Size: 416 53 83 399 24 1686 923 958
Cuts at: 4984 442
Size: 1753
Fragments arranged by size:
1753 1686 958 923 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2836 4955 1239
Size: 1073 524 2119 2579
Fragments arranged by size:
2579 2119 1073 524
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3379 4086 493
Size: 711 2175 707 2702
Fragments arranged by size:
2702 2175 711 707
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2156 2434 2546
Size: 53 83 186 936 539 84 278 112
Cuts at: 2546 2766 2979 3366 3469 4102 4484 5119 275
Size: 220 213 387 103 633 382 635 1451
Fragments arranged by size:
1451 936 635 633 539 387 382 278
220 213 186 112 103 84 83 53
BsaWI w'CCGG_w
Cuts at: 2566 4728 5126 5400 5547 5958 2566
Size: 2162 398 274 147 411 2903
Fragments arranged by size:
2903 2162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 3479 3509 1178
Size: 30 165 30 642 30 1404 30 3964
Fragments arranged by size:
3964 1404 642 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2223 2317 2801 2831 3391 1186
Size: 1037 94 484 30 560 4090
Fragments arranged by size:
4090 1037 560 484 94 30
BseYI C'CCAG_C
Cuts at: 2049 2107 2517 2877 3302 3322 3545 5498 5994
Size: 58 410 360 425 20 223 1953 496
Cuts at: 5994 2049
Size: 2350
Fragments arranged by size:
2350 1953 496 425 410 360 223 58
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3191 1163
Size: 573 1455 4267
Fragments arranged by size:
4267 1455 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2478 2567 2680 3166 3433 3745
Size: 275 186 477 89 113 486 267 312
Cuts at: 3745 4506 5534 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 486 477 312 275 267
186 113 89
BsiHKAI G_wGCw'C
Cuts at: 4356 4441 5512 4356
Size: 85 1071 5139
Fragments arranged by size:
5139 1071 85
BsmI GAATG_Cn'
Cuts at: 2236 3806 3899 2236
Size: 1570 93 4632
Fragments arranged by size:
4632 1570 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2148 2389 2399 2595 2742 3192
Size: 399 324 34 241 10 196 147 450
Cuts at: 3192 3204 3329 3360 3614 4356 4441 5512 1391
Size: 12 125 31 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 324 254 241
196 147 125 85 34 31 12 10
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3426 3650 844
Size: 2582 224 3489
Fragments arranged by size:
3489 2582 224
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 2325 2354 3592 4182 1394
Size: 429 455 47 29 1238 590 3507
Fragments arranged by size:
3507 1238 590 455 429 47 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 4790 4964 65
Size: 1183 3542 174 1396
Fragments arranged by size:
3542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2316 2480 2566 2637 3184 3313
Size: 110 293 213 164 86 71 547 129
Cuts at: 3313 3552 4939 5126 1700
Size: 239 1387 187 2869
Fragments arranged by size:
2869 1387 547 293 239 213 187 164
129 110 86 71
BsrGI T'GTAC_A
Cuts at: 96 3535 96
Size: 3439 2856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 2729 4355 5367 2729
Size: 1626 1012 3657
Fragments arranged by size:
3657 1626 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3187 794
Size: 2393 3902
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 2794 3625 3992 4377 4394 5846 5932 5944
Size: 822 831 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 831 822 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3034 3034
Size: 6295
BtgI C'CryG_G
Cuts at: 513 1065 2225 2379 2390 2453 3150 3361 3441
Size: 552 1160 154 11 63 697 211 80
Cuts at: 3441 513
Size: 3367
Fragments arranged by size:
3367 1160 697 552 211 154 80 63
11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 2778 3811 4605 4625 2128
Size: 650 1033 794 20 3798
Fragments arranged by size:
3798 1033 794 650 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 3214 3214
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2978 5302 809
Size: 507 1662 2324 1802
Fragments arranged by size:
2324 1802 1662 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2162 2475 3163
Size: 54 1425 50 275 18 332 313 688
Cuts at: 3163 3576 3742 4631 6260 8
Size: 413 166 889 1629 43
Fragments arranged by size:
1629 1425 889 688 413 332 313 275
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2475 3163 3742 1537
Size: 275 663 688 579 4090
Fragments arranged by size:
4090 688 663 579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2948 4225 1353
Size: 1595 1277 3423
Fragments arranged by size:
3423 1595 1277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 2984 4882 5256 5402 387
Size: 1064 138 492 903 1898 374 146 1280
Fragments arranged by size:
1898 1280 1064 903 492 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2836 4358 4955 5742 6010 1239
Size: 393 680 524 1522 597 787 268 1524
Fragments arranged by size:
1524 1522 787 680 597 524 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2732 2732
Size: 6295
EcoO109I rG'GnC_Cy
Cuts at: 2091 2111 2738 2739 2862 2091
Size: 20 627 1 123 5524
Fragments arranged by size:
5524 627 123 20 1
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 2014 2075 2366 2437 2501 2616 2878 3106
Size: 336 61 291 71 64 115 262 228
Cuts at: 3106 3352 3369 3472 5442 1678
Size: 246 17 103 1970 2531
Fragments arranged by size:
2531 1970 336 291 262 246 228 115
103 71 64 61 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 3017 3049 4936
Size: 32 135 32 1097 32 316 32 1887
Cuts at: 4936 4968 5010 5042 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1887 1097 316 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KasI G'GCGC_C
Cuts at: 2071 2433 3365 3468 2071
Size: 362 932 103 4898
Fragments arranged by size:
4898 932 362 103
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 2343 3291 6171 2343
Size: 948 2880 2467
Fragments arranged by size:
2880 2467 948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 5409 5593 1305
Size: 1482 2622 184 2007
Fragments arranged by size:
2622 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2164 3578 10
Size: 54 1425 343 332 1414 2727
Fragments arranged by size:
2727 1425 1414 343 332 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3377 4253 4612 4771 518
Size: 648 2211 876 359 159 2042
Fragments arranged by size:
2211 2042 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2271 2527 2536 2658 2825 3363 3443
Size: 621 44 256 9 122 167 538 80
Cuts at: 3443 3582 3711 4388 5536 5781 1606
Size: 139 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 538 256 245 167
139 129 122 80 44 9
NaeI GCC'GGC
Cuts at: 2105 2482 2639 2105
Size: 377 157 5761
Fragments arranged by size:
5761 377 157
NarI GG'CG_CC
Cuts at: 2072 2434 3366 3469 2072
Size: 362 932 103 4898
Fragments arranged by size:
4898 932 362 103
NcoI C'CATG_G
Cuts at: 513 1065 2379 2453 513
Size: 552 1314 74 4355
Fragments arranged by size:
4355 1314 552 74
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NgoMIV G'CCGG_C
Cuts at: 2103 2480 2637 2103
Size: 377 157 5761
Fragments arranged by size:
5761 377 157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 2475 3742 2475
Size: 1267 5028
NruI TCG'CGA
Cuts at: 2563 2563
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 3146 3653 5198 1132
Size: 97 1917 507 1545 2229
Fragments arranged by size:
2229 1917 1545 507 97
PasI CC'CwG_GG
Cuts at: 1348 2541 2760 1348
Size: 1193 219 4883
Fragments arranged by size:
4883 1193 219
PciI A'CATG_T
Cuts at: 3142 5194 3142
Size: 2052 4243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PfoI T'CCnGG_A
Cuts at: 3056 3056
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PmlI CAC'GTG
Cuts at: 3379 3379
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspOMI G'GGCC_C
Cuts at: 2110 2738 3325 2110
Size: 628 587 5080
Fragments arranged by size:
5080 628 587
PstI C_TGCA'G
Cuts at: 830 3647 830
Size: 2817 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2527 3582 2527
Size: 1055 5240
SacII CC_GC'GG
Cuts at: 2228 3364 3444 2228
Size: 1136 80 5079
Fragments arranged by size:
5079 1136 80
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2303 3643 4778 5459 5650 826
Size: 219 1258 1340 1135 681 191 1471
Fragments arranged by size:
1471 1340 1258 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2109 2109
Size: 6295
SfoI GGC'GCC
Cuts at: 2073 2435 3367 3470 2073
Size: 362 932 103 4898
Fragments arranged by size:
4898 932 362 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 2089 2116 2264 2868 2916 3113 3622 1872
Size: 217 27 148 604 48 197 509 4545
Fragments arranged by size:
4545 604 509 217 197 148 48 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 3039 1517
Size: 1522 4773
StyI C'CwwG_G
Cuts at: 513 1065 2379 2453 3512 3734 513
Size: 552 1314 74 1059 222 3074
Fragments arranged by size:
3074 1314 1059 552 222 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3070 4322 4339 4492 4677 5122 325
Size: 1255 1490 1252 17 153 185 445 1498
Fragments arranged by size:
1498 1490 1255 1252 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3535 4541 6253
Size: 274 80 33 51 1443 1558 1006 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
1712 1558 1443 1006 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 2127 2159 2449 2481 3479 3511 6255
Size: 32 50 32 290 32 998 32 2744
Cuts at: 6255 6287 2045
Size: 32 2053
Fragments arranged by size:
2744 2053 998 290 50 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 2087 2114 2262 2866 2914 3111 3620 1870
Size: 217 27 148 604 48 197 509 4545
Fragments arranged by size:
4545 604 509 217 197 148 48 27
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2980 4103 276
Size: 53 83 186 936 1446 1123 2468
Fragments arranged by size:
2468 1446 1123 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SrfI SwaI XcmI