(Linear) (Six-base) MAPSORT of: KIEE2214.seq Check: 9432 from: 1 to: 1590 KIEE2214.orf With 182 enzymes: SgfI * July 3, 2012 17:14 .. AatII G_ACGT'C Cuts at: 0 977 1590 Size: 977 613 AfeI AGC'GCT Cuts at: 0 359 871 1345 1590 Size: 359 512 474 245 Fragments arranged by size: 512 474 359 245 AflIII A'CryG_T Cuts at: 0 338 484 1137 1151 1286 1590 Size: 338 146 653 14 135 304 Fragments arranged by size: 653 338 304 146 135 14 AgeI A'CCGG_T Cuts at: 0 561 1590 Size: 561 1029 AhdI GACnn_n'nnGTC Cuts at: 0 678 1590 Size: 678 912 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1306 1590 Size: 1306 284 AlwNI CAG_nnn'CTG Cuts at: 0 656 1065 1254 1590 Size: 656 409 189 336 Fragments arranged by size: 656 409 336 189 ApaI G_GGCC'C Cuts at: 0 109 737 1324 1590 Size: 109 628 587 266 Fragments arranged by size: 628 587 266 109 AvaI C'yCGr_G Cuts at: 0 33 82 109 257 612 861 909 1106 Size: 33 49 27 148 355 249 48 197 Cuts at: 1106 1590 Size: 484 Fragments arranged by size: 484 355 249 197 148 49 48 33 27 BanI G'GyrC_C Cuts at: 0 66 138 314 381 428 1182 1305 1360 Size: 66 72 176 67 47 754 123 55 Cuts at: 1360 1463 1511 1590 Size: 103 48 79 Fragments arranged by size: 754 176 123 103 79 72 67 66 55 48 47 BanII G_rGCy'C Cuts at: 0 109 394 590 737 1199 1324 1590 Size: 109 285 196 147 462 125 266 Fragments arranged by size: 462 285 266 196 147 125 109 BbeI G_GCGC'C Cuts at: 0 70 432 1364 1467 1590 Size: 70 362 932 103 123 Fragments arranged by size: 932 362 123 103 70 BclI T'GATC_A Cuts at: 0 497 1590 Size: 497 1093 BglI GCCn_nnn'nGGC Cuts at: 0 104 178 379 1016 1590 Size: 104 74 201 637 574 Fragments arranged by size: 637 574 201 104 74 Bme1580I G_kGCm'C Cuts at: 0 109 143 384 737 1187 1324 1355 1590 Size: 109 34 241 353 450 137 31 235 Fragments arranged by size: 450 353 241 235 137 109 34 31 BmgBI CAC'GTC Cuts at: 0 485 1152 1590 Size: 485 667 438 Fragments arranged by size: 667 485 438 BmrI ACTGGGnnnn_n' Cuts at: 0 1098 1590 Size: 1098 492 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 915 1590 Size: 915 675 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 831 1590 Size: 307 524 759 Fragments arranged by size: 759 524 307 Bpu10I CC'TnA_GC Cuts at: 0 695 1590 Size: 695 895 BsaAI yAC'GTr Cuts at: 0 1374 1590 Size: 1374 216 BsaHI Gr'CG_yC Cuts at: 0 67 151 429 541 761 974 1361 1464 Size: 67 84 278 112 220 213 387 103 Cuts at: 1464 1590 Size: 126 Fragments arranged by size: 387 278 220 213 126 112 103 84 67 BsaWI w'CCGG_w Cuts at: 0 561 1590 Size: 561 1029 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 40 70 1474 1504 1590 Size: 40 30 1404 30 86 Fragments arranged by size: 1404 86 40 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 218 312 796 826 1386 1590 Size: 218 94 484 30 560 204 Fragments arranged by size: 560 484 218 204 94 30 BseYI C'CCAG_C Cuts at: 0 44 102 512 872 1297 1317 1540 1590 Size: 44 58 410 360 425 20 223 50 Fragments arranged by size: 425 410 360 223 58 50 44 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1186 1590 Size: 1186 404 BsiEI CG_ry'CG Cuts at: 0 473 562 675 1161 1428 1590 Size: 473 89 113 486 267 162 Fragments arranged by size: 486 473 267 162 113 89 BsmI GAATG_Cn' Cuts at: 0 231 1590 Size: 231 1359 Bsp1286I G_dGCh'C Cuts at: 0 109 143 384 394 590 737 1187 1199 Size: 109 34 241 10 196 147 450 12 Cuts at: 1199 1324 1355 1590 Size: 125 31 235 Fragments arranged by size: 450 241 235 196 147 125 109 34 31 12 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1421 1590 Size: 1421 169 BsrBI CCG'CTC Cuts at: 0 273 320 349 1587 1590 Size: 273 47 29 1238 3 Fragments arranged by size: 1238 273 47 29 3 BsrFI r'CCGG_y Cuts at: 0 98 311 475 561 632 1179 1308 1547 Size: 98 213 164 86 71 547 129 239 Cuts at: 1547 1590 Size: 43 Fragments arranged by size: 547 239 213 164 129 98 86 71 43 BsrGI T'GTAC_A Cuts at: 0 1530 1590 Size: 1530 60 BssSI C'ACGA_G Cuts at: 0 724 1590 Size: 724 866 BstAPI GCAn_nnn'nTGC Cuts at: 0 1182 1590 Size: 1182 408 BstYI r'GATC_y Cuts at: 0 789 1590 Size: 789 801 Bsu36I CC'TnA_GG Cuts at: 0 1029 1590 Size: 1029 561 BtgI C'CryG_G Cuts at: 0 220 374 385 448 1145 1356 1436 1590 Size: 220 154 11 63 697 211 80 154 Fragments arranged by size: 697 220 211 154 154 80 63 11 BtsI GCAGTG_nn' Cuts at: 0 123 773 1590 Size: 123 650 817 Fragments arranged by size: 817 650 123 DraIII CAC_nnn'GTG Cuts at: 0 1209 1590 Size: 1209 381 DrdI GACnn_nn'nnGTC Cuts at: 0 973 1590 Size: 973 617 EaeI y'GGCC_r Cuts at: 0 157 470 1158 1571 1590 Size: 157 313 688 413 19 Fragments arranged by size: 688 413 313 157 19 EagI C'GGCC_G Cuts at: 0 470 1158 1590 Size: 470 688 432 Fragments arranged by size: 688 470 432 EarI CTCTTCn'nnn_ Cuts at: 0 943 1590 Size: 943 647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 76 979 1590 Size: 76 903 611 Fragments arranged by size: 903 611 76 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 831 1590 Size: 307 524 759 Fragments arranged by size: 759 524 307 EcoNI CCTnn'n_nnAGG Cuts at: 0 727 1590 Size: 727 863 EcoO109I rG'GnC_Cy Cuts at: 0 86 106 733 734 857 1590 Size: 86 20 627 1 123 733 Fragments arranged by size: 733 627 123 86 20 1 HaeII r_GCGC'y Cuts at: 0 9 70 361 432 496 611 873 1101 Size: 9 61 291 71 64 115 262 228 Cuts at: 1101 1347 1364 1467 1590 Size: 246 17 103 123 Fragments arranged by size: 291 262 246 228 123 115 103 71 64 61 17 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 664 696 1012 1044 1590 Size: 664 32 316 32 546 Fragments arranged by size: 664 546 316 32 32 KasI G'GCGC_C Cuts at: 0 66 428 1360 1463 1590 Size: 66 362 932 103 127 Fragments arranged by size: 932 362 127 103 66 MluI A'CGCG_T Cuts at: 0 338 1286 1590 Size: 338 948 304 Fragments arranged by size: 948 338 304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 782 1590 Size: 782 808 MscI TGG'CCA Cuts at: 0 159 1573 1590 Size: 159 1414 17 Fragments arranged by size: 1414 159 17 MslI CAynn'nnrTG Cuts at: 0 1372 1590 Size: 1372 218 MspA1I CmG'CkG Cuts at: 0 222 266 522 531 653 820 1358 1438 Size: 222 44 256 9 122 167 538 80 Cuts at: 1438 1577 1590 Size: 139 13 Fragments arranged by size: 538 256 222 167 139 122 80 44 13 9 NaeI GCC'GGC Cuts at: 0 100 477 634 1590 Size: 100 377 157 956 Fragments arranged by size: 956 377 157 100 NarI GG'CG_CC Cuts at: 0 67 429 1361 1464 1590 Size: 67 362 932 103 126 Fragments arranged by size: 932 362 126 103 67 NcoI C'CATG_G Cuts at: 0 374 448 1590 Size: 374 74 1142 Fragments arranged by size: 1142 374 74 NgoMIV G'CCGG_C Cuts at: 0 98 475 632 1590 Size: 98 377 157 958 Fragments arranged by size: 958 377 157 98 NotI GC'GGCC_GC Cuts at: 0 470 1590 Size: 470 1120 NruI TCG'CGA Cuts at: 0 558 1590 Size: 558 1032 NspI r_CATG'y Cuts at: 0 1141 1590 Size: 1141 449 PasI CC'CwG_GG Cuts at: 0 536 755 1590 Size: 536 219 835 Fragments arranged by size: 835 536 219 PciI A'CATG_T Cuts at: 0 1137 1590 Size: 1137 453 PfoI T'CCnGG_A Cuts at: 0 1051 1590 Size: 1051 539 PmlI CAC'GTG Cuts at: 0 1374 1590 Size: 1374 216 PspOMI G'GGCC_C Cuts at: 0 105 733 1320 1590 Size: 105 628 587 270 Fragments arranged by size: 628 587 270 105 PvuII CAG'CTG Cuts at: 0 522 1577 1590 Size: 522 1055 13 Fragments arranged by size: 1055 522 13 SacII CC_GC'GG Cuts at: 0 223 1359 1439 1590 Size: 223 1136 80 151 Fragments arranged by size: 1136 223 151 80 SfcI C'TryA_G Cuts at: 0 298 1590 Size: 298 1292 SfiI GGCCn_nnn'nGGCC Cuts at: 0 104 1590 Size: 104 1486 SfoI GGC'GCC Cuts at: 0 68 430 1362 1465 1590 Size: 68 362 932 103 125 Fragments arranged by size: 932 362 125 103 68 SmaI CCC'GGG Cuts at: 0 84 111 259 863 911 1108 1590 Size: 84 27 148 604 48 197 482 Fragments arranged by size: 604 482 197 148 84 48 27 StuI AGG'CCT Cuts at: 0 1034 1590 Size: 1034 556 StyI C'CwwG_G Cuts at: 0 374 448 1507 1590 Size: 374 74 1059 83 Fragments arranged by size: 1059 374 83 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1065 1590 Size: 1065 525 TatI w'GTAC_w Cuts at: 0 1530 1590 Size: 1530 60 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 40 72 122 154 444 476 1474 1506 Size: 40 32 50 32 290 32 998 32 Cuts at: 1506 1590 Size: 84 Fragments arranged by size: 998 290 84 50 40 32 32 32 32 XmaI C'CCGG_G Cuts at: 0 82 109 257 861 909 1106 1590 Size: 82 27 148 604 48 197 484 Fragments arranged by size: 604 484 197 148 82 48 27 ZraI GAC'GTC Cuts at: 0 975 1590 Size: 975 615 Enzymes that do cut: AatII AfeI AflIII AgeI AhdI AlfI AlwNI ApaI AvaI BanI BanII BbeI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI PasI PciI PfoI PmlI PspOMI PvuII SacII SfcI SfiI SfoI SmaI StuI StyI TaqII TatI TstI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AflII AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmtI BpuEI BsaI BsaBI BsiHKAI BsiWI BsmBI BspEI BspHI BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI NdeI NheI NsiI PacI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI